Clinical Pathology and Laboratory Techniques for Veterinary Technicians

281 Pages·2015·10.812 MB·English

by  Anne M. Barger, Amy L. MacNeill

Clinical Pathology and
Laboratory Techniques for
Veterinary Technicians

Clinical Pathology and
Laboratory Techniques
for Veterinary
Technicians
Edited by
Anne M. Barger, DVM, MS, Diplomate ACVP
Clinical Professor, Clinical Pathology, University of Illinois
Amy L. MacNeill, DVM, PhD, Diplomate ACVP
Associate Professor, Clinical Pathology, Colorado State University
Thiseditionfirstpublished2015©2015byJohnWiley&Sons,Inc
Editorialoffices: 1606GoldenAspenDrive,Suites103and104,Ames,Iowa50010,USA
TheAtrium,SouthernGate,Chichester,WestSussex,PO198SQ,UK
9600GarsingtonRoad,Oxford,OX42DQ,UK
Fordetailsofourglobaleditorialoffices,forcustomerservicesandforinformationabouthowtoapplyfor
permissiontoreusethecopyrightmaterialinthisbookpleaseseeourwebsiteat
www.wiley.com/wiley-blackwell.
Authorizationtophotocopyitemsforinternalorpersonaluse,ortheinternalorpersonaluseofspecific
clients,isgrantedbyBlackwellPublishing,providedthatthebasefeeispaiddirectlytotheCopyright
ClearanceCenter,222RosewoodDrive,Danvers,MA01923.Forthoseorganizationsthathavebeen
grantedaphotocopylicensebyCCC,aseparatesystemofpaymentshasbeenarranged.Thefeecodesfor
usersoftheTransactionalReportingServiceareISBN-13:978-1-1183-4509-2/2015.
Designationsusedbycompaniestodistinguishtheirproductsareoftenclaimedastrademarks.Allbrand
namesandproductnamesusedinthisbookaretradenames,servicemarks,trademarksorregistered
trademarksoftheirrespectiveowners.Thepublisherisnotassociatedwithanyproductorvendor
mentionedinthisbook.
Thecontentsofthisworkareintendedtofurthergeneralscientificresearch,understanding,anddiscussion
onlyandarenotintendedandshouldnotberelieduponasrecommendingorpromotingaspecificmethod,
diagnosis,ortreatmentbyhealthsciencepractitionersforanyparticularpatient.Thepublisherandthe
authormakenorepresentationsorwarrantieswithrespecttotheaccuracyorcompletenessofthecontents
ofthisworkandspecificallydisclaimallwarranties,includingwithoutlimitationanyimpliedwarrantiesof
fitnessforaparticularpurpose.Inviewofongoingresearch,equipmentmodifications,changesin
governmentalregulations,andtheconstantflowofinformationrelatingtotheuseofmedicines,equipment,
anddevices,thereaderisurgedtoreviewandevaluatetheinformationprovidedinthepackageinsertor
instructionsforeachmedicine,equipment,ordevicefor,amongotherthings,anychangesintheinstructions
orindicationofusageandforaddedwarningsandprecautions.Readersshouldconsultwithaspecialist
whereappropriate.ThefactthatanorganizationorWebsiteisreferredtointhisworkasacitationand/ora
potentialsourceoffurtherinformationdoesnotmeanthattheauthororthepublisherendorsesthe
informationtheorganizationorWebsitemayprovideorrecommendationsitmaymake.Further,readers
shouldbeawarethatInternetWebsiteslistedinthisworkmayhavechangedordisappearedbetweenwhen
thisworkwaswrittenandwhenitisread.Nowarrantymaybecreatedorextendedbyanypromotional
statementsforthiswork.Neitherthepublishernortheauthorshallbeliableforanydamagesarising
herefrom.
LibraryofCongressCataloging-in-PublicationData
Clinicalpathologyandlaboratorytechniquesforveterinarytechnicians/editedbyAnneM.Bargerand
AmyL.MacNeill.
p.;cm.
Includesbibliographicalreferencesandindex.
ISBN978-1-118-34509-2(paperback)
1.Veterinaryclinicalpathology.I.Barger,AnneM.,editor.II.MacNeill,AmyL.,editor.
[DNLM:1.ClinicalLaboratoryTechniques–veterinary.2.AnimalTechnicians.3.Pathology,
Clinical–methods.4.Pathology,Veterinary–methods.SF772.6]
SF772.6.C552015
636.089′607–dc23
2015006610
AcataloguerecordforthisbookisavailablefromtheBritishLibrary.
Wileyalsopublishesitsbooksinavarietyofelectronicformats.Somecontentthatappearsinprintmaynot
beavailableinelectronicbooks.
Setin10/12ptSabonLTStdbyLaserwordsPrivateLimited,Chennai,India
1 2015
Amy MacNeill Dedication:
This book is dedicated to Dr. Bruce Ferguson, who taught me
many of the techniques in this text and nourished my love of
veterinary medicine.
Anne Barger Dedication:
I would to thank my partner in life Dr. Patty McElroy and my
father Maurice Barger for their continued love and support.

Contents
ListofContributors ix FurtherReading 71
Preface xi MultipleChoiceQuestions 74
AbouttheCompanionWebsite xiii
Chapter 3
Chapter 1
Hemostasis 75
GettingStartedwithClinical Introduction 77
Pathology 1 Physiology 77
Introduction 2 DiagnosticTesting 84
FurtherReading 91
StandardEquipment 2
MultipleChoiceQuestions 94
StandardSupplies 11
SampleTypes 14
SampleStorageandPreparation 15 Chapter 4
BasicLaboratorySafety 15
ClinicalChemistry 95
MultipleChoiceQuestions 17
Introduction 97
AvailableTesting 97
Chapter 2 SamplePreparation 97
SampleQuality 101
Hematology 19 ClinicalChemistryHealth
Introduction 21 Profiles 105
FurtherReading 138
ComponentsofPeripheralBlood 22
MultipleChoiceQuestions 140
BloodCellParameters 22
Erythrocytes 24
Chapter 5
Leukocytes 38
Platelets 50
Urinalysis 141
AtypicalCellsinthePeripheral
Introduction 142
Blood 53
UrineProduction 142
BloodParasites 54 FurtherReading 173
HematologyMethods 58 MultipleChoiceQuestions 175
vii
viii Contents
Chapter 6 LaboratoryConsiderations 228
LaboratoryArea 230
Parasitology 177 TypesofLaboratoryError 231
Introduction 179 Summary 242
ClassificationsofParasites 179 FurtherReading 242
ParasiteIdentification 213 MultipleChoiceQuestions 244
FurtherReading 224
MultipleChoiceQuestions 225 GlossaryofTerms 245
Index 255
Chapter 7
MinimizingLaboratoryErrorsin
VeterinaryPractice 227
Introduction 228