Table Of ContentCell Volume and Signaling
ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY
Editorial Board:
NATHAN BACK, State University of New York at Buffalo
IRUN R. COHEN, The Weizmann Institute of Science
DAVID KRITCHEVSKY, Wistar Institute
ABEL LAJTHA, N. S. Kline Institute for Psychiatric Research
RODOLFO PAOLETTI, University of Milan
Recent Volumes in this Series
Volume 551
POST GENOMIC PERSPECTIVES IN MODELING AND CONTROL OF BREATHING
Edited by Jean Champagnat, Monique Denavit-Saubie, Gilles Fortin, Arthur S. Foutz,
and Muriel Thoby-Brisson
Volume 552
TYPE I DIABETES: Biomedical Science and Technology
Edited by George Eisenbarth
Volume 553
BIOMATERIALS: From Molecules to Engineered Tissues
Edited by Nesrin Hasırcı and Vasıf Hasırcı
Volume 554
PROTECTING INFANTS THROUGH HUMAN MILK: Advancing the Scientific
Evidence
Edited by Larry K. Pickering, Ardythe L. Morrow, Guillermo M. Ruiz-Palacios,
and Richard J. Schanler
Volume 555
BREAST FEEEDING: Early Influences on Later Health
Edited by Gail Goldberg, Andrew Prentice, Ann Prentice, Suzanne Filteau,
and Elsie Widdowson
Volume 556
IMMUNOINFORMATICS: Opportunities and Challenges of Bridging Immunology with
Computer and Information Sciences
Edited by Christian Schoenbach, V. Brusic, and Akihiko Konagaya
Volume 557
BRAIN REPAIR
Edited by M. Ba¨hr
Volume 558
DEFECTS OF SECRETION IN CYSTIC FIBROSIS
Edited by Carsten Schultz
Volume 559
CELL VOLUME AND SIGNALING
Edited by Peter K. Lauf and Norma C. Adragna
A Continuation Order Plan is available for this series. A continuation order will bring delivery of each new volume
immediately upon publication. Volumes are billed only upon actual shipment. For further information please contact
the publisher.
Cell Volume and Signaling
Edited by
Peter K. Lauf
Wright State University
Dayton, Ohio
and
Norma C. Adragna
Wright State University
Dayton, Ohio
A C.I.P. Catalogue record for this book is available from the Library of Congress.
ISSN: 0065 2598
ISBN: 0-387-23299-0
Proceedings of the 2003 Dayton International Symposium on Cell Volume Regulation and Signaling
(cid:1)2004 Springer Science(cid:1)Business Media, Inc.
All rights reserved. This work may not be translated or copied in whole or in part without the written
permission of the publisher (Springer Science(cid:1)Business Media, Inc., 233 Spring Street, New York, NY
10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection
with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or
dissimilar methodology now known or hereafter developed is forbidden.
The use in this publication of trade names, trademarks, service marks and similar terms, even if they are not
identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to
proprietary rights.
Printed in the United States of America.
9 8 7 6 5 4 3 2
springeronline.com
FOREWORD
In front of you is the finished product of your work, the text of your contributions to
the 2003 Dayton International Symposium on Cell Volume and Signal Transduction. As
we all recall, this symposium brought together the Doyens of Cellular and Molecular
Physiology as well as aspiring young investigators and students in this field. It became a
memorable event in an illustrious series of International Symposia on Cell Volume and
Signaling. This series, started by Professors Vladimir Strbák, Florian Lang and Monte
Greer in Smolenice, Slovakia in 1997 and continued by Professors Rolf Kinne, Florian
Lang and Frank Wehner in Berlin in 2000, is projected for 2005 in Copenhagen to be
hosted by our colleague, Professor Else Hoffmann and her team.
We dearly miss Monte Greer to whom this symposium was dedicated and addressed
so eloquently by Vladimir Strbák in his Dedication to Monte. Monte and I became
friends in Smolenice and had begun to discuss the 2003 meeting only a few days before
his tragic accident in 2002. There are others who were not with us, and we missed them,
too.
We would not have been able to succeed in this event without the unflagging support
of our higher administration at Wright State University, the NIDDKD of the National
Institute of Health, and the Fuji Medical System (see Acknowledgments).
The special touch of our Symposium Coordinator / Editorial Assistant Donna Maas
was reflected in the management and organization of the symposium details, generation
of program highlights, and finally, as you all know from the many e-mails, in the hard
work thereafter to edit all our chapters readying them for final publication. We all owe
her a big thanks and round of applause.
In the final layout of this volume and the listing of its chapters, we adhered to the
original program which divided the symposium into four major topics at the cutting edge
of research in cell volume and signal transduction. There is an Abstract appendix
containing those abstracts that were expanded by some of the poster presenters.
At the concluding dinner of the Dayton International Symposium at the Dayton Art
Institute, our dear colleague and friend Hector Rasgados-Flores, physiologist and
composer, dedicated a Sarabanda for Cello and Piano which he together with our cellist
son Adrian Lauf performed. This fine piece of music is published herein for the first time
and interwoven between the chapters and abstracts.
Our special thanks to every member on the Editorial/Advisory Committee for
reviewing and editing each page of this book. On behalf of the members of this
committee and Wright State University, we would like to thank all who helped and our
colleagues and friends who came from near and far to make the 2003 Dayton
International Symposium on Cell Volume and Signal Transduction such a resounding
success.
For the Editorial/Advisory Committee,
Peter K. Lauf, Norma C. Adragna
University Professor Professor of Pharmacology & Toxicology
v
DEDICATION
Monte Arnold Greer
(1922-2002)
This Symposium is dedicated to the
memory of Monte Arnold Greer, pioneer in
the field of cell swelling-induced secretion,
exceptional scientist and rare friend, who
died on March 24, 2002 in an auto accident
in Oregon.
It was in the early eighties when Monte
noticed that an improperly prepared
perfusion solution was surprisingly effective
in the stimulation of pituitary hormone
secretion. Interestingly enough, secretion
returned to basal values and responded again
to different stimuli. It looked like a joke and that was enough to attract Monte’s attention.
He was unaware of any similar observations. Now, we know that some other people
noticed a similar behavior for one particular hormone, but it was Monte who was
thunderstruck by finding that it was a broad, universal phenomenon. And so this highly
reputed neuroendocrinologist spent the rest of his carrier studying and admiring cell
swelling-induced hormone secretion. Illustrative is the title of his paper: Greer MA, Greer
SE, Opsahl Z, McCafferty L, Maruta S: Hyposmolar stimulation of in vitro pituitary
secretion of luteinizing hormone: a potent clue to the secretory process, Endocrinology
113: 1531-1533,1983. Over the following years, Monte published many elegant papers in
prestigious scientific journals; however, he did not evoke an expected interest among
orthodox endocrinologists. In the years 1994-1998, we worked on a joint project
Isosmotic Ethanol-induced Neural Cell Swelling and Thyroliberin Release supported by
the US-Slovak Science and Technology Cooperation Program. During one of my visits in
Portland, Monte suggested contacting relevant people in various fields to organize a
symposium on cell swelling. We were impressed by the number of top scientists who
worked on cell volume regulation and were pleased that many of them attended our first
International Symposium on Primary Role of Cell Volume Changes in Controlling
Cell Function, in Smolenice Castle in Slovakia, June 23-27, 1997. As the main
organizers, Monte, Florian Lang and I served as chairpersons of the meeting. That was
the beginning of this series. It was followed by the International Conference on Cell
Volume: Signalling and Regulation, Max Plank Institute, Berlin, October 25-28, 2000,
and now, by the present International Symposium on Cell Volume & Signaling,
September 20-24, 2003, at Wright State University in Dayton, Ohio.
Monte Arnold Greer was born in Portland, Oregon; he received his A.B. in Biology
(1944) and M.D. (1947) at Stanford University. After working in Bethesda, Boston and
San Francisco, he accepted an offer as head of the Division of Endocrinology,
Department of Medicine, Oregon Health Sciences University, in Portland, Oregon, in
vii
viii DEDICATION
1956. Under his leadership (1956-1990), first as Associate Professor of Medicine (1956)
and then Professor of Medicine and Physiology (1962), the Endocrinology Division in
Portland developed into an eminent clinical and research institution.
Monte Greer was an excellent clinician and gifted researcher who pioneered work in
several fields. Most acknowledged has been the field of hypothalamic regulation of
pituitary hormone secretion. He was the first to publish proof of the role of
the hypothalamus in thyroid regulation – a lesion of the hypothalamus prevented goiter
development in experimental rats. Twenty-five years later, A. Shally and R. Guilemin
shared the Nobel Prize for the isolation of the thyrotropin-releasing hormone from the
hypothalamus. Naturally, Monte was included in the prestigious publication: Pioneers in
Neuroendocrinology (M. A. Greer: Why I am still waiting for the free trip to Stockholm,
chapter 13, Plenum Publishing Corporation, 1978).
Among the awards Monte Greer received were the Oppenheimer Award of the Endo-
crine Society and Honorary memberships in the Japanese and Czechoslovak Endocrine
Societies. Monte’s achievements were also recognized by his appropriate roles in
professional societies:
American Thyroid Association President
Endocrine Society Vice President
Western Society for Clinical Research President
American Society for Clinical research Chair, Subsection on Endocrinology
In addition, he served as a member of the editorial boards of Endocrinology, Neuroendo-
crinologyandEndocrinologia Experimentalis.
Monte’s charming personality, esprit, rare sense of humor and friendliness made him
popular with friends all over the world. His personality is best reflected in his statement:
“Nobody is as bad as his publications!” The sad message of his death deeply touched his
former research fellows and coworkers and their families in USA, Japan, Switzerland,
France, Germany, Slovakia, China and possibly other countries. We are all very pleased
to devote this exciting meeting to the memory of our friend Monte Arnold Greer.
Vladimir Strbak
ACKNOWLEDGMENTS
We want to express our gratitude for the unflagging support—moral and financial—
we received from Wright State University, in particular:
Dr. Perry Moore, Executive Vice President
Dr. David Hopkins, Provost
Dr. Joseph Thomas, Associate Provost
Dr. Howard Part, Dean, School of Medicine
Mr. John Bale, Associate Dean, School of Medicine
Dr. Robert Fyffe, Associate Dean for Research Affairs, School of Medicine
Dr. Michele Wheatly, Dean, College of Science and Mathematics
Dr. Gerald Alter, Biomedical Sciences Ph.D. Program
Dr. Arthur Pickoff, Department of Pediatrics, Children’s Medical Center
This symposium was supported by a grant from the National Institute of Diabetes,
Digestive and Kidney Diseases of the National Institutes of Health (1R13DK064886-
01).We would like to thank Program Director Dr. David Badman,for helping us achieve
the goals of this meeting.
In addition, our thanks go to Mr. Bob Coyne of Fuji Medical Systems for his
generous support of the 2003 Dayton International Symposium on Cell Volume
Regulation and Signaling.
ix
CONTENTS
FOREWORD.............................................................................................................. v
DEDICATION ............................................................................................................ vii
ACKNOWLEDGMENTS ......................................................................................... ix
1. THE BALANCING ACT OF THE NAKED CELL:
A BRIEF HISTORY OF MEMBRANE REGULATION
OF ANIMAL CELL VOLUME BEFORE 1978 ...................................... 1
John S. Willis (Pre-Symposium Lecturer)
ELECTRONEUTRAL COTRANSPORTERS
2. TWENTY-FIVE YEARS OF K-Cl COTRANSPORT:
FROM STIMULATION BY A THIOL REACTION TO
CLONING OF THE FULL-LENGTH KCCs .......................................... 11
Peter K. Lauf and Norma C. Adragna
3. MOLECULAR PHYSIOLOGY OF MAMMALIAN K+-CL-
COTRANSPORTERS ................................................................................. 29
Adriana Mercado, Gerardo Gamba, and David B. Mount
4. STE20 KINASES AND CATION-CHLORIDE
COTRANSPORTERS:AMINIREVIEW ............................................... 43
Eric Delpire
5. MOLECULAR PHYSIOLOGY OF THE RENAL
Na+:Cl- AND Na+:K+:2Cl- COTRANSPORTERS .................................... 55
Gerardo Gamba and Norma A. Bobadilla
xi
xii CONTENTS
6. THE ROLE OF THE BLOOD-BRAIN BARRIER Na-K-Cl
COTRANSPORTER IN STROKE: A Minireview .................................. 67
Martha E. O’Donnell, Tina I. Lam, Lien Tran and Steven E. Anderson
7. REGULATION OF Na-K-2Cl COTRANSPORT
IN RED CELLS ........................................................................................... 77
Peter Flatman
8. A NOVEL NHE1 FROM RED BLOOD CELLS OF THE
WINTER FLOUNDER: REGULATION BY MULTIPLE
SIGNALING PATHWAYS ........................................................................ 89
Stine F. Pedersen
ION CHANNELS AND EXCHANGERS
9. PROBING OF THE ICln CHANNEL PORE BY CYSTEINE
MUTAGENESIS AND CADMIUM-BLOCK .......................................... 99
M. Jakab, M. L. Garavaglia, J. Fürst, S. Rodighiero, F. Guizzardi,
G. Meyer, M. Ritter, and M. Paulmichl
10. VOLUME–DEPENDENT AND -INDEPENDENT ACTIVATED
ANION CONDUCTANCES AND THEIR INTERACTION
IN THE RENAL INNER MEDULLARY COLLECTING
DUCT (IMCD) ............................................................................................. 109
Stefan H. Boese, Mike A. Gray, and Nick L. Simmons
11. SECRETORY CONTROL OF BASOLATERAL MEMBRANE
POTASSIUM AND CHLORIDE CHANNELS IN COLONIC
CRYPT CELLS ........................................................................................... 119
Dan R. Halm
12. EFFECTS OF AMMONIUM ON ION CHANNELS AND
TRANSPORTERS IN COLONIC SECRETORY CELLS ..................... 131
Roger T. Worrell and Jeffrey B. Matthews
13. THE VOLUME-ACTIVATED CHLORIDE CURRENT
DEPENDS ON PHOSPHOLIPASE C ACTIVATION AND
INTRACELLULAR CALCIUM MOBILIZATION ............................... 141
D. Varela, F. Simon, A. Riveros, F. Jørgensen and A. Stutzin
14. NITRIC OXIDE (NO) MODULATION OF CL-DEPENDENT
TRANSPORTERS IN THE KIDNEY ....................................................... 147
Pablo A. Ortiz and Jeffrey L. Garvin
