Cell Adhesion in Bioprocessing and Biotechnology

290 Pages·1995·74.15 MB·English

by  Hjortso

CELL
ADHESION
BIOPROCESTSE CHNOLOGY
Series Ediror
W. Courtney McGregor
Xoma Corporation
Berkeley, California
1. Membrane Separations in Biotechnology, edited by W. Courtney
McGregor
2. Commercial Production of MonoclonalA ntibodies: A Guide for Scale-
Up , edited by Sally S. Seaver
3. Handbook on Anaerobic Fermentations, edited by Larry E. Erickson
and Daniel Yee-Chak Fung
4. Fermentation Process Development of Industrial Organisms, edited by
Justin 0. Neway
5. Yeast: Biotechnology and Biocatalysis, edited by Hubert Verachtert
and Red De Mot
6. Sensors in Bioprocess Control, edited by John V. Twork andAlexan-
der M. Yacynych
7. Fundamentals of Protein Biotechnology, edited by Stanley Stein
8. Yeast Strain Selection, edited by Chandra J. Panchal
9. Separation Processes in Biotechnology, edited by Juan A. Asenjo
IO. Large-scale Mammalian Cell Culture Technology, edited by Anthony
S. Lubiniecki
11. Extractive Bioconversions, edited by Bo Mattiasson and OIle Holst
12. Purification and Analysis of Recombinant Proteins, edited by Ramnath
Seetharam and Satish K. Sharma
13. Drug Biotechnology Regulation: Scientific Basis and Practices, edited
by Yuan-yuan H. Chiu and John L. Gueriguian
14. ProteinI m mobilizationF: undamentalsa ndA pplications, edited by
Richard F. Taylor
15. Biosensor Principles and Applications, edited by Lo2 J. Blum and
Pierre R. Coulet
16. IndustrialA pplicationo f Immobilized Biocatalysts, edited by Arsuo
Tanaka, Tetsuya Tosa, and Takeshi Kobayashi
17. Insect Cell Culture Engineering, edited by Mattheus F. A. Goosen,
Andrew J. Daugulis, and Peter Faulkner
18. Protein Purification Process Engineering, edited by Roger G. Harrison
19. RecombinantM icrobes for Industriala nd Agricultural Applications,
edited by Yoshikatsu Murooka and Tadayuki lmanaka
20. Cell Adhesion: Fundamentalsand Biotechnological Applications, edited
by Martin A. Hjortso and Joseph W. Roos
ADDITIONAL VOLUMES IN PREPARATION
Bioreactor System Design, edited by Juan A. Asenjo and Jose! C.
Merchuk
GeneE xpressioni nR ecombinantM icroorganisms, edited by Alan
Smith
This Page Intentionally Left Blank
CELL
Fundamentals and
Biotechnological Applications
edited by
Martin A. Hjortso
Louisiana State University
Baton Rouge, Louisiana
Joseph W. Roos
Ethyl Corporation
Richmond, Virginia
MarceD l ekker, Inc. NewY orkB . asel Hong Kong
Library of Congress Cataloging-in-Publication Data
Cell adhesion : fundamentals and biotechnological applications /
edited by Martin A. Hjortso, Joseph W. Roos.
p.c m. - (Bioprocesst echnology ; v.2 0)
Includes bibliographical references and index.
ISBN 0-8247-8945-8
1.C ella dhesion.2 .B ioreactors.3 .B iotechnology.
I. Hjortso, Martin A. 11. Roos, Joseph W.
111. Series.
TP248.25.C42C149 59 4
660’.63-d~20 94-22882
CIP
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Copyright 0 1995 by Marcel Dekker, Inc.A ll Rights Reserved.
Neither this book nor any part may be reproduced or transmitted in any
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system, without permission in writinfgr om thep ublisher.
Marcel Dekker, Inc.
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Current printing (last digit):
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PRINTED IN THEU NITED STATES OF AMERICA
Series Introduction
Bioprocess technology encompasses all of the basic and applied sciences as
well as the engineering required to fully exploit living systems and bring
their products to the marketplace. The technology that develops is eventu-
ally expressed in various methodologies and types of equipment and instru-
ments built up along a bioprocess stream. Typically in commercial produc-
tion, thes tream begins at theb ioreactor, which can bea classical fermentor,
a cell culture perfusion system, or ane nzyme bioreactor. Then comes sepa-
ration of the product from the living systems and/or their components
followed by an appropriate number of purification steps. The stream ends
with bioproduct finishing, formulation, andp ackaging. A given bioprocess
stream may have some tributaries or outlets and may be overlaid with a
variety of monitoring devices and control systems. As with any stream, it
will both shape and be shaped with time. Documenting the evolutionary
shaping of bioprocess technology ist he purpose of this series.
Now that several products from recombinant DNA and cell fusion tech-
niques are on the market, the new era of bioprocess technology is well
established and validated. Books of this series represent developments in
various segments of bioprocessing that have paralleled progress in the life
sciences. For obvious proprietary reasons, some developments in industry,
although validated, may be published only later, if at all. Therefore, our
continuing series will follow the growth of this field as it is available from
both academia and industry.
W. Courtney McGregor
ill
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Preface
Over the past several years, cell adhesion has progressedf rom a phenome-
non of peripheral interest to biochemical engineerst o become a useful tool
in bioreactor design and bioseparations. Along with thesei nnovations have
arisen increased demands for better understanding of the phenomena that
underlie cell adhesion. The aim of this book is to present the student or
researcher in biochemical engineering with an introduction to the basic
phenomena that govern cell adhesion and top rovide descriptions of bioen-
gineering processes in which cell adhesion plays an important part.
Cell adhesion hasa lready been studied extensively because ofi ts medical
significance. The ability of different cells to adhere or aggregate plays a
crucial role in suchp rocesses as microbial infections, spread ofc ancer
through the body, and plaque formation on teeth. However, the abundant
literature thath as come out of the biomedical research isn ot always partic-
ularly useful to the biochemical engineer, since the medical perspective is
much different from the engineering point of view. Engineering applica-
tions of cell adhesiona re presented int he research literature, butt o date no
comprehensive reference work has been available on this topic. In view of
this, it appeared timely to assemble a book dedicated to the engineering
aspects of cella dhesion.
To theb iochemical engineer, cell adhesion ias potential problem as well
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