Carotenoids Part A: Chemistry, Separation, Quantitation, and Antioxidation

574 Pages·1992·8.19 MB·English

by  Lester Packer (Eds.)

Contributors to Volume 213
elcitrA numbers are in sesehtnerap gniwollof the names of .srotubirtnoc
snoitailiffA listed era current.
TORUNN AAKERMANN (20), The Norwe- University of the Negev, Sede-Boker
gian Institute of Technology, Organic 84990, Israel
Chemistry Laboratories, The University ANDR~ M. BRAUN (39), D~partement de
of Trondheim, N-7034 Trondheim-Nth, Chimie, Institut de Chimie Physique,
Norway Ecole Polytechnique FOd~rale de Lau-
ALICE J. ADLER (19), Eye Research Insti- sanne, CH- I 51O Lausanne, Switzerland
tute, Boston, Massachusetts 02114 ALAN H. BRUSH (29), Department of Physi-
MORDHAY AVRON* (41), Department of ology and Neurobiology, University of
Biochemistry, The Weizmann Institute of Connecticut, Storrs, Connecticut 06268
Sciences, Rehovot 76100, Israel WALTER BURGER (5), Roche Research
ARUN B. BARUA (24), Department of Bio- Center, Hoffman-LaRoche Inc., Nutley,
chemistry and Biophysics, Iowa State Uni- New Jersey 07110
versity, Ames, Iowa 50011 L. M. CANFIELD (37), Department of Bio-
GARY R. BELCHER (12, 18, 32), Nutrient chemistry, University of Arizona, Tucson,
Composition Laboratory, Beltsville Hu- Arizona 85721
man Nutrition Research Center, U.S. SATISH C. CHOUDHRY (5), Roche Research
Department of Agriculture, Agricultural Center, Hoffman-LaRoche Inc., Nutley,
Research Service, Beltsville, Maryland New Jersey 07110
20705
ANDREW J. CLIFFORD (25), Department of
AMI BEN-AMOTZ (41), Israel Oceanographic Nutrition, Univeristy of California, Davis,
and Limnological Research, Tel-Shik- Davis, California 95616
mona, Haifa 31080, Israel
THERESE M. COTTON (4), Department of
H. ROBERT BERGEN, III (6), Department of Chemistry, Ames Laboratory, Iowa State
Chemistry, University of Wisconsin-- La University, Ames, Iowa 50011
Crosse, La Crosse, Wisconsin 54601
NEAL E. CRAFT (17), Chemical Science and
CLAUDETTE BERSET (13), Laboratoire Pig- Technology Laboratory, Organic Analyti-
ments Naturels et Couleur des Aliments, cal Research Division, National Institute
D~partement Science de l'Aliment, Ecole of Standards and Technology, Gaithers-
Nationale Sup~rieure des Industries, Ag- burg, Maryland 20899
ricoles et Alimentaires, 91305 Massy,
J. CROUZET (7), Centre de G~nie et de Tech-
France
nologie Alimentaires lnstitut des Sciences
PETER BEYER (8), Institut ff~r Biologie II, de l'Ingenieur, Universit~ de Montpellier
Universittit Freiburg, D-7800 Freiburg, II, F-34095 Montpellier Cedex ,50
Germany France
RICHARD A. BONE (33), Department of JOSEPH CUPANO (5), Roche Research
Physics, Florida International University, Center, Hoffman-LaRoche Inc., Nutley,
Miami, Florida 33199 New Jersey 07110
SAMMY BOUSSlBA (36), Micro-algal Biotech- CHARLES E. DAITCH (18), Nutrient Compo-
nology Laboratory, The Jacob Blaustein sition Laboratory, Beltsville Human Nu-
Institute for Desert Research, Ben Gurion trition, Research Center, U.S. Department
of Agriculture, Agricultural Research Ser-
*Deceased. vice, Beltsville, Maryland 20705
ix
X CONTRIBUTORS TO VOLUME 213
A. P. DE REEHNEEL (22), Laboratoria your pool L69 3BX, England
Medische Biochemie en your Klinische An-
SNEVED GUST (10), Department of Chemis-
alyse, Universiteit Gent, 0009-11 Gent, Bel-
try and Biochemistry, Center for Early
gium
Events in Photosynthesis, Arizona State
THOMAS P. A. MAYAGASAVED (40), Bio- .ytisrevinU Tempe, Arizona 85287
yrtsimehc Division, Bhabha Atomic Re- GARRY J. HANDELMAN (19, 31), Depart-
search Center, Bombay, India
ment of Nutrition, University of ,ainrofilaC
OLOAP 1D OICSAM (40), Instituto de Qui- Davis, Davis, California 95616
mica, University of DaS Paulo, San Paulo, HANS-JORGEN HANSEN (39), -hcsinagrO
Brazil Chemisches Institut, Universitdt Ziirich,
DRAWDE A. DRATZ (19), Department of CH-8057 Zarich, Switzerland
Chemistry and Biochemistry, Montana ELRAJ ANDR~ HAUGAN (20), The Norwe-
State University, Bozeman, Montana gian Institute of Technology, Organic
59717 Chemistry Laboratories, The University
of Trondheim, N-7034 Trondheim-Nth,
Lu NAF (36), Micro-algal Biotechnology
Norway
Laboratory, The Jacob Blaustein Institute
NYLECOJ HUDON (29), Department of -reteV
for Desert Research, Ben Gurion Univer-
inary Anatomy, The University of Sas-
sity of the Negev, Sede-Boker 84990, Israel
katchewan, Saskatoon, Saskatchewan,
J. P. UOZ~lR~lF (9), Ecole Normale Supdri- Canada S7N OWO
eure, Laboratoire de Chimie, 75231 Paris
IHCI-NUJ USDIHSI (34), Biological Labora-
Cedex ,50 France
tory, Nippon Medical School Kawasald
GEORGE W. FRANCIS (21), Department of 211, Japan
Chemistry, University of Bergen, N-5007 IHSOYASAM OTI (2), Kobe Women's Col-
Bergen, Norway lege of Pharmacy, Kobe &56 Japan
HARRY A. FRANK (28), Department of A. DANIEL JONES (25), decnavdArofytilicaF
Chemistry, University of Connecticut, Instrumentation, University of ,ainrofilaC
Storrs, Connecticut 06269 Davis, Davis, California 95616
MIHCAOJ-SNAH NEBELSIERF (44), Klinikum VALERIAN E. KAGAN (44), Department of
der Johann Goethe-Universitdt, Gustav- Molecular and Cell Biology, University of
Embden-Zentrum der Biologischen Che- ,ainrofilaC Berkeley, Berkeley, California
mie, Abt. Mikrobiologische Chemie, 6000 94720
Frankfurt/Main, Germany
P. DUWASANAK (7), Department of Chemis-
DLORAH C. FURR (24, 25), Department of try, Chiang Mai University, Chiang Mai,
Nutritional Sciences, University of Con- Thailand 50002
necticut, Storrs, Connecticut 06269 B. E. YLLEK (37), Department of Biochemis-
A. R. GIULIANO (37), Department of Bio- try, University of Arizona, Tucson, Ari-
chemistry, University of Arizona, Tucson, zona 85 127
Arizona 85721 TODD A. KENNEDY (45), Department of
IRIGALDUM B. GOLI (18, 32), Center for Pharmacology and Toxicology, College of
Drug Design and Development, College of Pharmacy, University of Arizona, Tucson,
Pharmacy, University of Toledo, Toledo, Arizona 85721
Ohio 43606 KCIREDERF KHACHIK (12, 18, 32), Nutrient
R. G6MEZ (11), Department of Biochemistr) Composition Laboratory, Beltsville Hu-
and Molecular Biology, University of the man Nutrition Research Center, U.S. De-
Basque Country, 48080 Bilbao, Spain partment of Agriculture, Agricultural Re-
T. W. NIWDOOG (15), Department of Bio- search Service, Beltsville, Maryland 20705
chemistry, University of Liverpool, Liver- JAE-Ho KIM (4), Department of Chemistry,
CONTRIBUTORS TO VOLUME 213 xi
Ames Laboratory, Iowa State ,ytisrevinU Department of Medicine, Channing Labo-
Ames, Iowa 50011 ,yrotar Harvard Medical School Boston,
HANS KLEINm (8), lnstitut F~r Biologie II, Massachusetts 02115
Universitdt Freiburg, D-7800 Freiburg, TAKAO MATSUNO (3), Department of Natu-
Germany ral Products Research, Kyoto Pharmaceu-
YASUSm KOYAMA (27), Faculty of ,ecneicS tical University, Kyoto 607, Japan
Kwansei Gakuin University, Uegahara, J. C. G. MILICUA (ll), Department of Bio-
Nishinomiya 662, Japan chemistry and Molecular Biology, Univer-
NAMRON I. KRINSKY (23, 31, 38), Depart- sity of the Basque Country, 48080 Bilbao,
ment of Biochemistry, Tufts University Spain
School of Medicine, Boston, Massachu- ANAL. MOORE (10), Department of Chem-
setts 02111 istry and Biochemistry, Center for Early
Events in Photosynthesis, Arizona State
JOHN T. LANDRUM (33), Department of
,ytisrevinU Tempe, Arizona 85287
Chemistry, Florida International Univer-
sity, Miami, Florida 33199 SAMOHT A. MOORE (10), Department of
SEMAJ LAU (12), Analytical Instruments Di- Chemistry and Biochemistry, Center for
vision, Hewlett-Packard, Paramus, New Early Events in Photosynthesis, Arizona
Jersey 07652 State University, Tempe, Arizona 85287
EVONNYS NESNEJ-NEAAIL (20), The Norwe- AIC1RTAP 1DRAUS-OCCESARUM (39), De-
gian Institute of Technology, Organic partement de Chimie, Institut de Chimie
Chemistry Laboratories, The University Physique, Ecole Polytechnique elar~d~F de
of Trondheim, N-7034 Trondheim-Nth, Lausanne, CH-IO15 Lausanne, Switzer-
land
Norway
LUAP A. LIDDELL (10), Department of ArdHIKO NAGAO (43), National Food Re-
Chemistry and Biochemistry, Center for search Institute, Ministry of Agriculture,
Early Events in Photosynthesis, Arizona Forestry and Fishery, Tsukuba, Ibaraki
State University, Tempe, Arizona 85287 305, Japan
DANIEL C. LIEBLER (45), Department of E. M. NEILSON (37), Department of Bio-
Pharmacology and Toxicology, College of chemistry, University of Arizona, Tucson,
Pharmacy, University of Arizona, Tucson, Arizona 85721
Arizona 85721 H. J. NELIS (22), Laboratoria voor Farma-
DLONRA A. NAMBEIL (5), Roche Research ceutische Microbiologie, Universiteit Gent,
Center, Hoffman-LaRoche Inc., Nutley, B-9000 Gent, Belgium
New Jersey 07110 ESTHER OLIVEROS (39), Ddpartement de
BOEY PENG LIM (43), School of Chemical Chimie, lnstitut de Chimie Physique,
Sciences, Universiti SHins Malaysia, USM Ecole Polytechnique Fbdbrale de Lau-
Penang 11800, Malaysia sanne, CH- I 51O Lausanne, Switzerland
MAILLIW R. YBSUL (12, ,81 32), Insect Neu- ENITSUGUA S. H. ONG (14), Malaysian
robiology and Hormone Laboratory, U.S. Palm Oil Promotion Council 50450 Kuala
Department of ,erutlucirgA Agriculture Re- Lumpur, Malaysia
search Service, Beltsville, Maryland 20705 RETSEL REKCAP (44), Department of Molec-
CLAIRE MARTY (13), Laboratoire Pigments ular and Cell Biology, University of
Naturels et Couleur des Aliments, Dbpar- ,ainrofilaC Berkeley, Berkeley, California
tement Science de l'Aliment, Ecole Nation- 94720
ale Sup~rieure des Industries, Agricoles et
ALOAP PALOZZA (38), Istituto di Patologia
Alimentaires, 91305 Massy, France
Generale, Universita Cattolica del Sacro
ENILEHCIM i. MATHEWS-ROTH (23, 46), Cuore, Roma 01168, Italy
xii CONTRIBUTORS TO VOLUME 213
DONG-Fd PARK (43), Department of Bio- HELMUT SIES (40), Institut Jar Physiolo-
chemistry, Kon-Kok University, Chung-Ju, gische Chemie ,1 Universitdt D~sseldo~
Korea D-4000 Df~sseldorf l, Germany
RETEPSNAH REDNAFP (1), Institute of -rO LEAHCIM G. SIMIC (42), Department of
ganic Chemistry, University of Bern, CH- Pharmacology and Toxicology, School of
3012 Bern, Switzerland Pharmacy, University of Baltimore, Balti-
LEAFAR LEROCIP (4), Department of Plant more, Maryland 21201
Nutrition, Estaci6n Experimental Aula D. XAM YLREDDONS (19), Eye Research In-
DeL 50080 Zaragoza, Spain stitute, Boston, Massachusetts 02114
MARK D. TTESSUR (19), Department of Bio- DERFLA R. TSIUQDNUS (40), Institut far
chemistry, Tufts University School of Med- Physiologische Chemie ,1 t~itisrevinU -s~fD
,enici Boston, Massachusetts 02111 ,modles D-4000 D~seldorf l, Germany
DLORAH H. ZTIMHCS (30), Department of SmNICHI IHCXAKAT (34, 35), Biological
Food Science, College of Agricultural and Laboratory, Nippon Medical School, Ka-
Life Sciences, North Carolina State Uni- wasaki 211, Japan
,ytisrev Raleigh, North Carolina 27695 E. .S TEE (14), Division of Human Nutrition,
NEVETS J. ZTRAWHCS (30), Department of Institute for Medical Research, 50588
Food Science, College of Agricultural and Kuala Lumpur, Malaysia
Life Sciences, North Carolina State Uni- JuNJl TERAO (43), National Food Research
,ytisrev Raleigh, North Carolina 27695 Institute, Ministry of Agricuhure, Forestry
OIGROIG SCITA (16, 44), Department of Nu- and Fishery, Tsukuba, Ibaraki 305, Japan
trition Sciences, University of ,ainrofilaC OKIHASAM AYIHCUST (44), Department of
Berkeley, Berkeley, California 94720 Molecular and Cell Biology, University of
MICHAEL SEIBERT (4), Photoconversion ,ainrofilaC Berkeley, Berkeley, California
Branch, National Renewable Energy Lab- 94720
,yrotaro Golden, Colorado 80401 IHSOYIK ADIKUST (26), Kobe Women's Col-
Avlv HSIAHS (41), Department of Biochem- lege of Pharmacy, Kobe 658, Japan
istry, The Weizmann Institute of Science, DRAHCIR .B NAV NEMEERB (30), Depart-
Rehovot 76100, Israel ment of Chemistry, North Carolina State
AUHGNIB SHEN (31), Department of Bio- ,ytisrevinU Raleigh, North Carolina 27695
chemistry, Tufts University School of Med-
DAGIVA KAHSNOV (36), Micro-algal Bio-
,enici Boston, Massachusetts 02111
technology Laboratory, The Jacob Blaus-
YUKO ATABIHS (2), Kobe Women's College tein Institute for Desert Research, Ben
of Pharmacy, Kobe 658, Japan Gurion University of the Negev, Sede-
KEIZO ADAMIHS (35), Department of Biol- Boker 84990, lsrael
ogy, Tokyo Metropolitan University, Ha- OKIMUY ONAMAY (2), Kobe Women's Col-
chiohjL Tokyo 192-03, Japan lege of Pharmacy, Kobe 658, Japan
Preface
Carotenoids are unique pigments synthesized in photosynthetic higher
plants and photosynthetic microorganisms which serve essential functions
in the protection against singlet oxygen-generated damage by photosensi-
tized reactions. In the plant they participate in light-harvesting reactions
and may act as a protective covering for certain higher plant species. In
animals, carotenoids have attracted considerable interest, beginning over
fifty years ago when it was discovered that they are precursors for vitamin
A and its derivatives. Mammalian species do not synthesize carotenoids or
vitamin A. These essential substances are therefore derived solely from
plant carotenoids, which are absorbed and stored in tissues and subse-
quently metabolized.
From a biomedical and biotechnological viewpoint, the carotenoids
have also received considerable attention recently. In numerous epidemio-
logical studies, the dietary intake of foods rich in carotenoids, but not of
preformed vitamin A, has been associated with a reduced risk of some
types of cancer. Chemical studies have shown that the carotenoids are very
powerful quenchers of singlet oxygen and other reactive oxygen species.
Carotenoids, which are considered to be safe and stable, are currently
used primarily as colorants in food products such as margarine. However,
there is increasing interest in their possible role in maintaining health
particularly in regard to lowering the risk of cancer. Many multivitamin
supplements now contain beta carotene.
In addition to being of widespread biomedical interest, the carotenoids
and their biosynthetic pathways are also being considered as targets for
precise inhibitors that would act as herbicides.
Studies of the genetics and molecular biology of carotenoids in micro-
bial photosynthetic systems, or nonphotosynthetic organisms such as
Escherichia coli, are leading to the identification of the gene sequence
homologies and differences between microbial and higher plant systems.
This area of genetics and molecular and cell biology is undergoing rapid
development. It is suspected that carotenoids, or perhaps their metabolic
products, may act as regulators of gene expression.
In addition to research on their natural occurrence, structure, and
biological activity, the distribution of carotenoids in animal and plant
tissues has also been receiving considerable attention.
xiii
xiv ECAFERP
These developments have led to an explosion of activity in this research
area. This volume and its companion Volume 214 of Methods ni Enzy-
mology present a comprehensive and state-of-the-art compilation of the
molecular and cellular methodologies needed for pursuing research with
carotenoids.
RETSEL REKCAP
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
Edited by YENDIS P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV II. Preparation and Assay of Enzymes
Edited by YENDIS P. KClWOLOC DNA NATHAN O. NALPAK
EMULOV III. Preparation and Assay of Substrates
Edited by YENDIS P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV IV. Special Techniques fort he Enzymologist
Edited by YENDIS P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV V. Preparation and Assay of Enzymes
Edited by YENDIS P. KClWOLOC DNA NATHAN O. NALPAK
EMULOV VI. Preparation and Assay of Enzymes (Continued)
Preparation and Assay of Substrates
Special Techniques
Edited by YENDIS P. KClWOLOC DNA NATHAN O. KAPLAN
EMULOV VII. Cumulative Subject Index
Edited by YENDIS P. KClWOLOC DNA NATHAN O. KAPLAN
EMULOV VIII. Complex Carbohydrates
Edited by HTEBAZILE F. NEUFELD DNA VICTOR GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited by WILLIS A. WOOD
EMULOV X. Oxidation and Phosphorylation
Edited by RONALD W. KOORBATSE DNA MAYNARD E. NAMLLUP
EMULOV XI. Enzyme Structure
Edited by C. H. W. HIRS
EMULOV XII. Nucleic Acids (Parts A and B)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XIII. Citric Acid Cycle
Edited by J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited by J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited by DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
Edited by KUSTIN KENNETH
VX
xvi
METHODS IN ENZYMOLOGY
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited by TREBREH TABOR DNA CELIA WHITE ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited by DONALD B. McCoRMICK DNA LEUMEL D. WRIGHT
EMULOV XlX. Proteolytic Enzymes
Edited by GERTRUDE E. NNAMLREP DNA OLZSAL DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KtVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA K/VIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited by WILLIAM B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited by YNOHTNA NAS PIETRO
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by YNOHTNA NAS PIETRO
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by VICTOR GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXII. Biomembranes (Part B)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by MARTHA G. DENNIS DNA DRAWDE A. SINNED
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited by WILLIAM B. YBOKAJ DNA MEIR WILCHEK
EMULOV XXXV. Lipids (Part B)
Edited by JOHN M. NIETSNEWOL
SDOHTEM IN YGOLOMYZNE xvii
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. HARDMAN
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. HARDMAN
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited yb JOEL G. HARDMAN DNA BERT W. O'MALLEY
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and Organ
Systems)
Edited by JOEL G. HARDMAN DNA BEST W. O'MALLEY
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by BEST W. O'MALLEY AND JOEL G. HARDMAN
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited by W. A. WOOD
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited by W. A. WOOD
EMULOV XLIII. Antibiotics
Edited by JOHN H. HASH
EMULOV XLIV. Immobilized Enzymes
Edited by KLAUS HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited by OLZSAL DNAROL
EMULOV XLVI. Affinity Labeling
Edited by WILLIAM B. YBOKAJ DNA MEIR WILCHEK
EMULOV XLVII. Enzyme Structure (Part E)
Edited by C. H. W. Hiss DNA EGRES N. FFEHSAMIT
EMULOV XLVIII. Enzyme Structure (Part F)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLIX. Enzyme Structure (Part G)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV L. Complex Carbohydrates (Part C)
Edited by VICTOR GRUBSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited by AICIRTAP A. HOFFEE DNA MARY ELLEN SENOJ
EMULOV LII. Biomembranes (Part C: Biological Oxidations)
Edited yb SIDNEY FLEISCHER DNA LESTER PACKER
EMULOV LIII. Biomembranes (Part D: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LESTER PACKER
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LESTER PACKER
XVlll °°° SDOHTEM IN YGOLOMYZNE
EMULOV LV. Biomembranes (Part F: Bioenergetics)
Edited by SIDNEY FLEISCHER AND LESTER PACKER
EMULOV LVI. Biomembranes (Part G: Bioenergetics)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV LVII. Bioluminescence and Chemiluminescence
Edited by ENELRAM A. ACULED
EMULOV LVIII. Cell Culture
Edited by WILLIAM B. JAKOBY DNA IRA PASTAN
EMULOV ElK. Nucleic Acids and Protein Synthesis (Part G)
Edited by EIVaK MOLDAVE AND LAWRENCE GROSSMAN
EMULOV LX, Nucleic Acids and Protein Synthesis (Part H)
Edited by ElVaK EVADLOM DNA ECNERWAL NAMSSORG
EMULOV 61. Enzyme Structure (Part H)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV 62. Vitamins and Coenzymes (Part D)
detidE yb DONALD B. McCoRMICK AND LEMUEL D. WRIGHT
EMULOV 63. Enzyme Kinetics and Mechanism (Part A: Initial Rate and Inhibitor
Methods)
Edited by LEINAD L. HCIRUP
EMULOV 64. Enzyme Kinetics and Mechanism (Part B: Isotopic Probes and
Complex Enzyme Systems)
Edited by LEINAD L, HCIROP
EMULOV 65. Nucleic Acids (Part I)
Edited by LAWRENCE GROSSMAN DNA KIVIE MOLDAVE
EMULOV 66. Vitamins and Coenzymes (Part E)
Edited by DONALD B. McCORMICK AND LEMUEL D. WRIGHT
EMULOV 67. Vitamins and Coenzymes (Part F)
Edited by DLANOD B. McCORMICK DNA LEUMEL D. WRIGHT
EMULOV 68. Recombinant DNA
Edited by YAR Wu
EMULOV 69. Photosynthesis and Nitrogen Fixation (Part C)
Edited by YNOHTNA NAS ORTEIP
EMULOV 70. Immunochemical Techniques (Part A)
Edited by HELEN VAN VUNAKIS AND JOHN J. LANGONE
EMULOV 71. Lipids (Part C)
Edited by JOHN M. NIETSNEWOL
EMULOV 72. Lipids (Part D)
Edited by JOHN M. NIETSNEWOL