Table Of ContentCALCIUM IN
BIOLOGICAL
SYSTEMS
CALCIUM IN
BIOLOGICAL
SYSTEMS
Edited by
Ronald P. Rubin
Medical College of Virginia
Virginia Commonwealth University
Richmond, Virginia
George 8. Weiss
CIBA-GEIGY Corporation
Summit, New Jersey
and
James W. Putney, Jr.
Medical College of Virginia
Virginia Commonwealth University
Richmond, Virginia
PLENUM PRESS • NEW YORK AND LONDON
Library of Congress Cataloging in Publication Data
Main entry under title:
Calcium in biological systems.
Based in part upon the proceedings of the 67th Annual Meeting of the Federation of
American Societies for Environmental Biology, held in Chicago, Ill., Apr. 10-15, 1983,
and sponsored by the American Society for Pharmacology and Experimental
Therapeutics and others.
Includes bibliographies and index.
1. Calcium - Physiological effect - Congresses. 2. Calcium - Metabolism - Congress
es. 3. Calcium-Metabolism-Disorders-Congresses. 4. Calcification-Congresses. 1.
Rubin, Ronald P. II. Weiss, George B. III. Putney, James W. IV. Federation of
American Societies for Experimental Biology. Meeting (67th: 1983: Chicago, Ill.) V.
American Society for Pharmacology and Experimental Therapeutics. [DNLM: 1. Cal
cification, Physiological-congresses. 2. Calcinosis-congresses. 3. Calcium-con
gresses. 4. Calcium Channel Blockers-therapeutic use-congresses. 5. Muscle Con
traction - drug effects - congresses. 6. Myocardial Contraction - drug effects - con
gresses. QV 276 C14297 1983]
QP535.C2C26325 1985 599/.019214 84-18246
ISBN-l3: 978-1-4612-9453-5 e-ISBN-13: 978-1-4613-2377-8
DOl: 10.1 007/978-1-4613-2377-8
© 1985 Plenum Press, New York
Softcover reprint of the hardcover 1st edition 1985
A Division of Plenum Publishing Corporation
233 Spring Street, New York, N.Y. 10013
All rights reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted
in any form or by any means, electronic, mechanical, photocopying, microfilming,
recording, or otherwise, without written permission from the Publisher
Contributors
Paul R. Adams • Department of Neurobiology and Behavior, State University of New York,
Stony Brook, New York 11794
Bernard W. Agranoff • Departments of Biologicaf Chemistry and Psychiatry, University of
Michigan, Ann Arbor, Michigan 48109
Myles H. Akabas • Departments of Physiology and Biophysics and Neuroscience, Albert Einstein
College of Medicine, Bronx, New York 10461
W. Almers • Department of Physiology and Biophysics, University of Washington, Seattle, Wash
ington 98195
H. Clarke Anderson • Department of Pathology and Oncology, University of Kansas Medical
Center, Kansas City, Kansas 66103
Robert J. Bache • Cardiovascular Division, Department of Medicine, University of Minnesota
Medical School, Minneapolis, Minnesota 55455
Edward M. Barr • Division of Pharmacology, College of Pharmacy, The University of Texas at
Austin, Austin, Texas 78712
S. M. Baylor • Department of Physiology, University of Pennsylvania, Philadelphia, Pennsylvania
19104
Robert E. Beamish • Experimental Cardiology Section, Department of Physiology, University of
Manitoba, Winnipeg, Manitoba R3E OW3, Canada
Gustavo Benaim • Laboratory of Biochemistry, Swiss Federal Institute of Technology (ETH),
8092 Zurich, Switzerland; permanent address: Universidad Central de Venezuela, Facultad de
Ciencias, Escuela de Biologia, Apartado 21201, Departamento de Biologia Celular, Caracas,
Venezuela
Michael J. Berridge • A.F.R.C. Unit of Insect Neurophysiology and Pharmacology, Department
of Zoology, University of Cambridge, Cambridge CB2 3EJ, England
John A. Bevan • Department of Pharmacology, School of Medicine, University of Vermont,
Burlington, Vermont 05405
Mordecai P. Blaustein • Department of Physiology, University of Maryland, School of Medi
cine, Baltimore, Maryland 21201
Adele L. Boskey • Department of Biochemistry, Cornell University Medical College, and Depart
ment of Ultrastructural Biochemistry, The Hospital for Special Surgery, New York, New York
10021
Charles Eric Brown • Department of Biochemistry, The Medical College of Wisconsin, Mil
waukee, Wisconsin 53226
David A. Brown • Department of Pharmacology, School of Pharmacology, London WCIN lAX,
England
v
vi Contributors
Hellmut Brunner • Biological Research Laboratories, Pharmaceuticals Division, Ciba-Geigy
Corporation, CH 4000 Basel, Switzerland
Daniel B. Burnham • Department of Physiology, University of California, San Francisco, Cal
ifornia 94143
Lewis Cantley • Department of Biochemistry and Molecular Biology, Harvard University,
Cambridge, Massaclrusetts 02138
Ernesto Carafoli • Laboratory of Biochemistry, Swiss Federal Institute of Technology (ETH),
8092 Zurich, Switzerland
P. L. Carlen • Addiction Research Foundation Clinical Institute, Playfair Neuroscience Unit,
Toronto Western Hospital, Departments of Medicine (Neurology), Physiology, and Institute of
Medical SciencF, University of Toronto, Toronto, Ontario M5S 2S 1, Canada
R. Casteels • Laboratory of Physiology, Catholic University of Louvain, Gasthuisberg Campus,
B3000 Louvain, Belgium
L. Judson Chandler • Division of Pharmacology, College of Pharmacy, The University of Texas
at Austin, Austin, Texas 78712
Herman S. Cheung • Division of Rheumatology, Department of Medicine, Medical College of
Wisconsin, Milwaukee, Wisconsin 53226
Haing U. Choi • Orthopedic Research Laboratories, Montefiore Medical Center, Bronx, New
York 10467
Robert B. Clark • Department of Physiology and Biophysics, University of Texas Medical
Branch, Galveston, Texas 77550
Fredric S. Cohen • Department of Physiology, Rush Medical College, Chicago, Illinois 60612
Jay N. Cohn • Cardiovascular Division, Department of Medicine, University of Minnesota Medi
cal School, Minneapolis, Minnesota 55455
Dennis L. Coleman • Department of Pharmaceutics, University of Utah, Salt Lake City, Utah
84112
Andrew Constanti • Department of Pharmacology, School of Pharmacology, London WCIN
lAX, England
J. J. Corcoran • Department of Pharmacology, Duke University Medical Center, Durham, North
Carolina 27710
Leoluca Criscione • Biological Research Laboratories, Pharmaceuticals Division, Ciba-Geigy
Corporation, CH 4000 Basel, Switzerland
James L. Daniel • Temple University, Thrombosis Research Center, Philadelphia, Pennsylvania
19104
Laura C. Daniell • Division of Pharmacology, College of Pharmacy, The University of Texas at
Austin, Austin, Texas 78712
Hector F. DeLuca • Department of Biochemistry, University of Wisconsin, Madison, Wisconsin
53706
Mrinal K. Dewanjee • Section of Nuclear Medicine, Mayo Clinic and Foundation, Rochester,
Minnesota 55905
Naranjan S. Dhalla • Experimental Cardiology Section, Department of Physiology, University of
Manitoba, Winnipeg, Manitoba R3E OW3, Canada
Sue K. Bolitho Donaldson • Department of Physiology and Department of Medical Nursing,
Rush University, Chicago, Illinois 60612
David E. Dong • Department of Pharmaceutics, University of Utah, Salt Lake City, Utah 84112
H. H. Draper • Department of Nutrition, College of Biological Science, University of Guelph,
Guelph, Ontario NIG 2Wl, Canada
G. Droogmans • Laboratory of Physiology, Catholic University of Louvain, Gathuisberg Cam
pus, B-3000 Louvain, Belgium
Robert Dunn, Jr. • Department of Physiology and Department of Medical Nursing, Rush Univer
sity, Chicago, Illinois 60612
Rosemary Dziak • Department of Oral Biology, State University of New York at Buffalo, Buf
falo, New York 14226
Contributors vii
Joseph Eichberg • Department of Biochemical and Biophysical Sciences, University of Houston,
Houston, Texas 77004
Leigh English • Department of Biochemistry and Molecular Biology, Harvard University,
Cambridge, Massachusetts 02138
Alexandre Fabiato • Department of Physiology and Biophysics, Medical College of Virginia,
Richmond, Virginia 23298
John N. Fain • Section of Biochemistry, Division of Biology and Medicine, Brown University,
Providence, Rhode Island 02912
Marc Fallert • Biological Research Laboratories, Pharmaceuticals Division, Ciba-Geigy Corpora
tion, CH 4000 Basel, Switzerland
Joseph J. Feher • Department of Physiology and Biophysics, Medical College of Virginia,
Richmond, Virginia 23298
Clare Fewtrell • Department of Pharmacology, New York State College of Veterinary Medicine,
Cornell University, Ithaca, New York 14853, and Section on Chemical Immunology, Arthritis
and Rheumatism Branch, National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases,
National Institutes of Health, Bethesda, Maryland 20205
Alan Finkelstein • Departments of Physiology and Biophysics and Neuroscience, Albert Einstein
College of Medicine, Bronx, New York 10461
A. Fleckenstein • Physiological Institute, University of Freiburg, 7800 Freiburg, West Germany
Gisa Fleckenstein-Grun • Physiological Institute, University of Freiburg, 7800 Freiburg, West
Germany
Hoger 8. Galburt • Department of Restorative Dentistry, Tufts University, School of Dental
Medicine, Boston, Massachusetts 02111
Stanley M. Gam • Center for Human Growth and Development and the Epidemiology Depart
ment of the School of Public Health, University of Michigan, Ann Arbor, Michigan 48109
Melvin J. Glimcher • Laboratory for the Study of Skeletal Disorders and Rehabilitation, Depart
ment of Orthopedic Surgery, Harvard Medical School, Children's Hospital Medical Center,
Boston, Massachusetts 02115
M. E. Gnegy • Department of Pharmacology, University of Michigan, Ann Arbor, Michigan
48109
T. Godfraind • Laboratory of Pharmacodynamics and Pharmacology, Catholic University uf Lou
vain, B-1200 Brussels, Belgium
P. P. Godfrey • Division of Cellular Pharmacology, Medical College of Virginia, Richmond,
Virginia 23298
H. Gonzalez-Serratos • Department of Biophysics, University of Maryland School of Medicine,
Baltimore, Maryland 21201
Paul Greengard • Tl>e Rockefeller University, New York, New York 10021
N. Gurevich • Addiction Research Foundation Clinical Institute, Playfair Neuroscience Unit,
Toronto Western Hospital, Departments of Medicine (Neurology), Physiology, and Institute of
Medical Science, University of Toronto, Toronto, Ontario M5S 2S I, Canada
Erik Gylfe • Department of Medical Cell Biology, Biomedicum, University of Uppsala, S-751 23
Uppsala, Sweden
T. J. Hallam • Physiological Laboratory, University of Cambridge, Cambridge CB2 3EG,
England
Hiroaki Harasaki • Department of Artificial Organs, Cleveland Clinic Foundation, Cleveland,
Ohio 44106
Charles A. Harrington • Analytical Neurochemistry Laboratory, Texas Research Institute of
Mental Sciences, Houston, Texas 77030
R. Adron Harris • Denver Veterans Administration Hospital and Department of Pharmacology,
University of Colorado, School of Medicine, Denver, Colorado 80262
Marguerite Hawley • Department of Cardiology and Laboratory of Human Biochemistry, Chil
dren's Hospital Medical Center, Boston, Massachusetts 02115
viii Contributors
Victor M. Hawthorne • Center for Human Growth and Development and the Epidemiology
Department of the School of Public Health, University of Michigan, Ann Arbor, Michigan 48109
80 Hellman • Department of Medical Cell Biology, Biomedicum, University of Uppsala, S-751
23 Uppsala, Sweden
Kent Hermsmeyer • Department of Pharmacology, The Cardiovascular Center, University of
Iowa, Iowa City, Iowa 52242
Holm Holmsen • Department of Biochemistry, University of Bergen, N-5000 Bergen, Norway
Susan L. Howard • Department of Cardiology and Laboratory of Human Biochemistry, Chil
dren's Hospital Medical Center, Boston, Massachusetts 02115
David S. Howell • Department of Medicine, University of Miami, School of Medicine, and U.S.
Veterans Administration, Miami, Florida 33101
Daniel A. Huetteman • Department of Physiology and Department of Medical Nursing, Rush
University, Chicago, Illinois 60612
Chiu Shuen Hui • Department of Biological Sciences, Purdue University, West Lafayette, Indi
ana 47907
Hwei-Chuen Hsu • Department of Pharmaceutics, University of Utah, Salt Lake City, Utah
84112
Joyce J. Hwa • Department of Pharmacology, School of Medicine, University of Vermont, Bur
lington, Vermont 05405
Shiro Kakiuchi • Department of Neurochemistry and Neuropharmacology, Institute of Higher
Nervous Activity, Osaka University Medical School, Nakanoshima, Kita-ku, Osaka 530, Japan
N. Kirshner • Department of Pharmacology, Duke University Medical Center, Durham, North
Carolina 27710
T. Kitazawa • The Pennsylvania Muscle Institute and Departments of Physiology and Pathology,
University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104; present ad
dress: Department of Pharmacology, Juntendo University School of Medicine, Tokyo 113, Japan
Claude B. Klee • Laboratory of Biochemistry, National Cancer Institute, National Institutes of
Health, Bethesda, Maryland 20205
Szloma Kowarski • Department of Physiology, Columbia University College of Physicians and
Surgeons, New York, New York 10032
Lois B. Kramer • Metabolic Section, Veterans Administration Hospital, Hines, Illinois 60141
Dieter M. Kramsch • Cardiovascular Institute, Boston University School of Medicine, Boston,
Massachusetts 02118
Hans Kilhnis • Biological Research Laboratories, Pharmaceuticals Division, Ciba-Geigy Corpo
ration, CH 4000 Basel, Switzerland
Yvonne Lai • The Rockefeller University, New York, New York 10021
Eduardo G. Lapetina • Department of Molecular Biology, The Wellcome Research Laborato
ries, Burroughs Wellcome Company, Research Triangle Park, North Carolina 27709
Steven W. Leslie • Division of Pharmacology, College of Pharmacy, The University of Texas at
Austin, Austin, Texas 78712
Robert Levenson • Department of Biology and Center for Cancer Research, Massachusetts In
stitute of Technology, Cambridge, Massachusetts 02139
Judith T. Levy • Department of Chemistry, Wellesley College, Wellesley, Massachusetts 02025
Robert J. Levy • Department of Cardiology and Laboratory of Human Biochemistry, Children's
Hospital Medical Center and Department of Pediatrics, Harvard Medical School, Boston, Mas
sachusetts 02115
Jane B. Lian • Department of Biological Chemistry (Orthopedic Surgery), Laboratory for the
Study of Skeletal Disorders and Rehabilitation, Harvard Medical School, Children's Hospital
Medical Center, Boston, Massachusetts 02115
Sue-Hwa Lin • Section of Biochemistry, Division of Biology and Medicine, Brown University,
Providence, Rhode Island 02912
Leona Ling • Department of Biochemistry and Molecular Biology, Harvard University,
Cambridge, Massachusetts 02138
Contributors ix
Irene Litosch • Section of Biochemistry, Division of Biology and Medicine, Brown University,
Providence, Rhode Island 02912
Ian G. Macara • Department of Biochemistry and Molecular Biology, Harvard University,
Cambridge, Massachusetts 02138; present address: Department of Radiation Biology and Bio
physics, University of Rochester School of Medicine, Rochester, New York 14642
David A. McCarron • Division of Nephrology and Hypertension, Oregon Health Sciences Uni
versity, Portland, Oregon 97201
Daniel J. McCarty • Division of Rheumatology, Department of Medicine, Medical College of
Wisconsin, Milwaukee, Wisconsin 53226
G. McClellan • The Pennsylvania Muscle Institue and Departments of Physiology and Pathology,
University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104
E. W. McCleskey • Department of Physiology and Biophysics, University of Washington, Seattle,
Washington 98195
Robert L. Macdonald • Departments of Neurology and Physiology, University of Michigan, Ann
Arbor, Michigan 48109
Teresa L. McGuinness • The Rockefeller University, New York, New York 10021
James T. McMahon • Department of Pathology, Cleveland Clinic Foundation, Cleveland, Ohio
44106
Allan S. Manalan • Laboratory of Biochemistry, National Cancer Institute, National Institutes of
Health, Bethesda, Maryland 20205
Gretchen S. Mandel • Department of Medicine and Research Service, Veterans Administration
Medical Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53193
Neil S. Mandel • Department of Medicine and Research Service, Veterans Administration Medi
cal Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53193
Johanne Martel-Pelletier • Rheumatic Disease Unit, Notre Dame Hospital, University of
Montreal, Montreal, Quebec H2L 4K8, Canada
Max Meier • Biological Research Laboratories, Pharmaceuticals Division, Ciba-Geigy Corpora
tion, CH 4000 Basel, Switzerland
Elias K. Michaelis • Neurobiology, Department of Human Development, and Center for Bio
medical Research, University of Kansas, Lawrence, Kansas 66045
Mary L. Michaelis • Neurobiology, Department of Human Development, and Center for Bio
medical Research, University of Kansas, Lawrence, Kansas 66045
Sara Morales • Department of Medicine, University of Miami, School of Medicine, Miami,
Florida 33101
Ofelia Muniz • Department of Medicine, University of Miami, School of Medicine, Miami,
Florida 33101
Paul H. Naccache • Department of Pathology, University of Connecticut Health Center, Farm
ington, Connecticut 06032
Abraham E. Nizel • Department of Oral Health Services, Tufts University, School of Dental
Medicine, Boston, Massachusetts 02111
Yukihiko Nose • Department of Artificial Organs, Cleveland Clinic Foundation, Cleveland, Ohio
44106
M. O'Beirne • Addiction Research Foundation Clinical Institute, Playfair Neuroscience Unit,
Toronto Western Hospital, Departments of Medicine (Neurology), Physiology, and Institute of
Medical Science, University of Toronto, Toronto, Ontario M5S 2S1, Canada
Donald B. Olsen • Department of Surgery, Division of Artificial Organs, University of Utah, Salt
Lake City, Utah 84112
Lauren Oshry • Department of Chemistry, Wellesley College, Wellesley, Massachusetts 02025
Charles C. Ouimet • The Rockefeller University, New York, New York 10021
Mary P. Owen • Department of Pharmacology, School of Medicine, University of Vermont,
Burlington, Vermont 05405
P. T. Palade • Department of Physiology and Biophysics, University of Washington, Seattle,
Washington 98195; present address: Department of Physiology and Biophysics, The University of
Texas Medical Branch, Galveston, Texas 77550
x Contributors
Genaro M. A. Palmieri • Department of Internal Medicine, University of Tennessee Center for
the Health Sciences, Memphis, Tennessee 38163
Vincenzo Panagia • Department of Oral Biology, University of Manitoba, Winnipeg, Manitoba
R3E OW3, Canada
C. Owen Parkes • Department of Physiology, University of British Columbia, Vancouver, British
Columbia V6T lW5, Canada
Jean Pierre Pelletier • Rheumatic Disease Unit, Notre Dame Hospital, University of Montreal,
Montreal, Quebec H2L 4K8, Canada
A. Robin Poole • Joint Diseases Research Laboratory, Shriners Hospital for Crippled Children,
Montreal, Quebec H3G lA6, Canada
Paul A. Price • Department of Biology, University of California at San Diego, La Jolla, California
92093
Walter C. Prozialeck • Department of Physiology and Pharmacology, Philadelphia College of
Osteopathic Medicine, Philadelphia, Pennsylvania 19131
J. W. Putney, Jr. • Division of Cellular Pharmacology, Medical College of Virginia, Richmond,
Virginia 23298
M. E. Quinta-Ferreira • Department of Physiology, University of Pennsylvania, Philadelphia,
Pennsylvania 19104; present address: Departmento de Fisica, Universidade de Coimbra, 3000
Coimbra, Portugal
Howard Rasmussen • Department.s of Cell Biology and Internal Medicine, Yale University,
New Haven, Connecticut 06510
T. J. Rink • Physiological Laboratory, University of Cambridge, Cambridge CB2 3EG, England
Harald Rogg • Biological Research Laboratories, Pharmaceuticals Division, Ciba-Geigy Corpora
tion, CH 4000 Basel, Switzerland
Gary S. Rogoff • Department of Complete Denture Prosthetics, Tufts University, School of
Dental Medicine, Boston, Massachusetts 02111
Lawrence C. Rosenberg • Orthopedic Research Laboratories, Montefiore Medical Center,
Bronx, New York 10467
Philip M. Rosoff • Department of Biochemistry and Molecular Biology, Harvard University,
Cambridge, Massachusetts 02138
Ronald P. Rubin • Division of Cellular Pharmacology, Medical College of Virginia, Richmond,
Virginia 23298
A. Sanchez • Physiological Laboratory, University of Cambridge, Cambridge CB2 3EG, England
Kiriti Sarkar • Department of Pathology, School of Medicine, Faculty of Health Sciences, Univer
sity of Ottawa, Ottawa, Ontario KIH 8M5, Canada
Leslie Satin • Department of Neurobiology and Behavior, State University of New York, Stony
Brook, New York 11794
David Schachter • Department of Physiology, Columbia University College of Physicians and
Surgeons, New York, New York 10032
Frederick J. Schoen • Department of Pathology, Brigham and Women's Hospital, Boston, Mas
sachusetts 02115
Jeffrey S. Schwartz • Cardiovascular Division, Department of Medicine, University of Min
nesota Medical School, Minneapolis, Minnesota 55455
Edward B. Seguin • Departments of Biological Chemistry and Psychiatry, University of Michi
gan, Ann Arbor, Michigan 48109
Ramadan I. Sha 'ati • Department of Physiology, University of Connecticut Health Center, Farm
ington, Connecticut 06032
Wolfgang Siess • Department of Molecular Biology, The Wellcome Research Laboratories,
Burroughs Well come Company, Research Triangle Park, North Carolina 27709; present address:
Medizinische Klinik Innenstadt der Universitat Miinchen, 8 Munich 2, West Germany
E. M. Silinsky • Department of Pharmacology, Northwestern University Medical School, Chi
cago, Illinois 60611
Pawan K. Singal • Experimental Cardiology Section, Department of Physiology, University of
Manitoba, Winnipeg, Manitoba R3E OW3, Canada
