Table Of ContentAdvances in Experimental Medicine and Biology 898
Juan A. Rosado Editor
Calcium Entry
Pathways in
Non-excitable
Cells
Advances in Experimental Medicine
and Biology
Volume 898
Editorial Board
IRUN R. COHEN, The Weizmann Institute of Science, Rehovot, Israel
N.S. ABEL LAJTHA, Kline Institute for Psychiatric Research, Orangeburg,
NY, USA
JOHN D. LAMBRIS, University of Pennsylvania, Philadelphia, PA, USA
RODOLFO PAOLETTI, University of Milan, Milan, Italy
More information about this series at h ttp://www.springer.com/series/5584
Juan A. Rosado
Editor
Calcium Entry Pathways
in Non-excitable Cells
Editor
Juan A. Rosado
Departamento de Fisiología
University of Extremadura
Cáceres , Spain
ISSN 0065-2598 ISSN 2214-8019 (electronic)
Advances in Experimental Medicine and Biology
ISBN 978-3-319-26972-6 ISBN 978-3-319-26974-0 (eBook)
DOI 10.1007/978-3-319-26974-0
Library of Congress Control Number: 2016939014
© Springer International Publishing Switzerland 2016
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Dedicated to Marcos and Maria
Pref ace
Cytosolic calcium homeostasis is a crucial event in cell biology as this ion acts as a
second messenger for a large number of cellular functions. The observations of
Ringer, published in the J ournal of Physiology in the early 1880s, revealed the
importance of calcium in cell physiology but specially focused the attention of the
researchers on the essential role of extracellular calcium entry. This issue has
attracted the attention of an increasing number of basic scientists interested in cel-
lular biology as well as biomedical researchers and clinicians interested in the
pathophysiological implications of calcium entry. Since this is a highly dynamic
topic, recapitulation of the historic and current knowledge is usually helpful to
understand the concepts and stimulate new approaches and directions that allow
signifi cant advances in various aspects of calcium infl ux.
After the assumption that Ca2 + infl ux was a major event in cell biology, voltage-
dependent calcium currents were identifi ed, and, later on, a mechanism for calcium
infl ux operated by the occupation of membrane receptors was reported both in elec-
trically excitable and non-excitable cells. Soon after the identifi cation of the so-
called receptor-operated calcium entry, the experimental observations indicated that
this process was not a unique mechanism, at least concerning the signaling pathway
underlying calcium channel gating. Despite that the occupation of membrane recep-
tors by physiological agonists is a common feature for all the mechanisms of
receptor- operated calcium entry, the subsequent activation of calcium-permeable
channels involves three different pathways. The simplest mechanism involves direct
opening of a receptor-operated channel where agonist-receptor binding leads to a
conformational change that results in calcium infl ux. The other two signaling routes
involve indirect channel gating either via generation of a diffusible second messen-
ger that is able to activate channel opening by itself (second messenger-operated
calcium entry) or by the discharge of the intracellular calcium stores (store-operated
or capacitative calcium entry). The term capacitative calcium entry was proposed by
James Putney Jr. to refer to a process whereby the discharge of the intracellular
calcium stores within a cell secondarily activates calcium entry into the cell across
the plasma membrane. During the past decades, a number of research groups have
explored the mechanisms underlying the activation, maintenance, and termination
vii
viii Preface
of the different routes for calcium entry in electrically excitable and non-excitable
cells, as well as the identity of the molecular components. Concerning the latter, the
different members of the Orai and TRPC families of channels have been found to
play a relevant role both for capacitative calcium entry and for other calcium infl ux
pathways. Given the importance of calcium infl ux in cell biology, one would expect
that dysfunction of this process might underlie serious pathological consequences.
In fact, a number of human disorders have been reported to involve Orai- as well as
TRPC-associated channelopathies.
Finally I would like to express my sincere thanks and appreciation to all con-
tributors for their kindly dedicated collaboration in the elaboration of this book and
also to Ginés M. Salido for his support editing this book. I would also like to thank
Thijs van Vlijmen, from Springer, for his encouraging support in the production of
this book.
I hope that the content of this book will be helpful for both PhD students and
advanced researchers interested in this fascinating fi eld as well as inspiring for basic
and biomedical scientists working in the pathophysiological implications of the
calcium entry pathways.
Cáceres, Spain Juan A. Rosado
October 2015
Contents
Part I Store-Operated Calcium Entry
1 Historical Overview of Store-Operated Ca2+ Entry ............................. 3
Letizia Albarran , Jose J. Lopez , Ginés M. Salido ,
and Juan A. Rosado
2 The STIM1: Orai Interaction ................................................................ 25
Irene Frischauf , Marc Fahrner , Isaac Jardín ,
and Christoph Romanin
3 The TRPCs, Orais and STIMs in ER/PM Junctions ........................... 47
Dong Min Shin , Aran Son , Seonghee Park , Min Seuk Kim ,
Malini Ahuja , and Shmuel Muallem
4 TRP-Na+/Ca2+ Exchanger Coupling ...................................................... 67
Alan G. S. Harper and Stewart O. Sage
5 Role of TRPC Channels in Store-Operated Calcium Entry ............... 87
Hwei Ling Ong , Lorena Brito de Souza , and Indu S. Ambudkar
6 Phospholipase A as a Molecular Determinant
2
of Store-Operated Calcium Entry ......................................................... 111
Tarik Smani , Alejandro Domínguez-Rodriguez , Paula Callejo-García ,
Juan A. Rosado , and Javier Avila-Medina
7 Extracellular Calcium Has Multiple Targets
to Control Cell Proliferation .................................................................. 133
Thierry Capiod
8 Regulation of Platelet Function by Orai, STIM and TRP ................... 157
Alejandro Berna-Erro , Isaac Jardín , Tarik Smani ,
and Juan A. Rosado
ix
