Table Of ContentProceedings in Life Sciences
A Specialized Symposium Organized for the
European Society for
Comparative Physiology and Biochemistry
in Crans-sur-Sierre, Switzerland, September 16-18, 1986
Conference Organization
Scientific Organizer: Ch. Gerday, Liege (Belgium)
General Organizers: L. Bolis, Milano (Italy), R. Gilles, Liege (Belgium)
Under the Patronage of
Fidia Research Laboratories
European Society for Comparative Physiology and Biochemistry
Ch. Gerday L. Balis R. Gilles (Eds.)
Calcium and
Calcium Binding Proteins
Molecular and Functional Aspects
With 157 Figures
Springer-Verlag
Berlin Heidelberg New York
London Paris Tokyo
Dr. CHARLES GERDAY
Laboratory of Muscle Biochemistry, Institute of Chemistry B6,
University of Liege, Sart Tilman, 4000 Liege, Belgium
Professor Dr. L. BOLls
Institute of General Physiology, University of Messina, Via dei Verdi 85,
98100 Messina, Italy
Professor Dr. R. GILLES
Laboratory of Animal Physiology, University of Liege, 22,
quai Van Beneden, 4020 Liege, Belgium
ISBN-13: 978-3-642-73044-3 e-ISBN-13: 978-3-642-73042-9
DOl: 10.1007/978-3-642-73042-9
Library of Congress Cataloging in Publication Data. Calcium and calcium binding proteins:
molecular and functional aspects/Ch. Gerday, L. Bolis, and R. Gilles (eds.). p. cm. - (Proceedings
of life sciences) "A specialized symposium organized for the European Society for Comparative
Physiology and Biochemistry in Crans-sur-Sierre, Switzerland, September 16-18, 1986" -P. facing
t. p. Includes index. 1. Calcium-binding proteins - Congresses. 2. Calcium-Physiological effect -
Congresses. I. Gerday, Charles. II. Bolis, Liana. III. Gilles, R. IV. European Society for
Comparative Physiology and Biochemistry. V. Series. [DNLM: 1. Calcium-Binding Proteins -
physiology - congresses. 2. Calcium - metabolism - congresses. 3. Calmodulin - Binding
Proteins - physiology - congresses. 4. Cells - physiology - congresses. QU 55 C1438 1986)
QP552.C24C34 1988 599:019214 - dc 19 DNLM/DLC for Library of Congress 87-32207 CIP
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Softcover reprint of the hardcover 1st edition 1988
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2131/3130-543210
Preface
The idea of Professors Bolis and Gilles to gather together for a 3 days'
meeting in the splendid environment of Crans-Montana in Switzerland a
limited number of people around the subject of calcium and calcium bind
ing proteins seemed at first particularly attractive, and when they asked me
to take charge of the scientific organization of the symposium, I accepted
with enthusiasm.
It rapidly became clear that the major problem would be the selection
of the topics, since it was impossible to cover completely and in depth such
a broad and dynamic area of research. In our view, one imperative was to
associate as intimately as possible the structural and the functional aspects
of the areas covered. Apart from one whole day focused on the fascinating
roles played by calmodulin in cellular activities, the other sessions were
devoted to calmodulin-related calcium binding proteins in muscle and non
muscle tissues and to some selected biological systems such as mitochondria,
secretory cells or sarcoplasmic reticulum in which calcium also plays a
crucial role.
The presentations were made by leading investigators in their field. Some
of them do not, however, appear in the present volume, for which there are
two reasons: first, some of the contributions were somewhat outside the
scope of the book; second, three speakers, for valid reasons, simply found
no opportunity to write a manuscript in the allotted time.
When analyzing the topics developed herein, one can obviously marvel
at the progress which has been made recently at the level of the molecular
characterization of calcium binding proteins, but also deplore that their
role in cell functioning often remains incompletely understood, evasive,
or simply unknown, as in the case of several EF hand calcium binding
proteins.
Although the meeting has only covered a limited number of topics and
has probably raised many more questions than it gives answers, it has
offered us the opportunity of evaluating the path we have still to follow
to reach an understanding of the role of calcium and its targets in cellular
activities.
I should like to thank all the participants, invited speakers, and those
who presented posters, for the high level of their contribution.
VI Preface
I also express my gratitude to Uana Bolis, Professor at the University of
Milano, for providing the essential support and raising the necessary funds
that rendered the organization of this symposium possible.
U~ge, February 1988 Charles Gerday
Contents
Introductory Lecture
Inositol Lipids, G-Proteins and Signal Transduction
S. Cockcroft (With 2 Figures) . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Calcium Binding Proteins in Muscle Tissues
Soluble Calcium Binding Proteins in Vertebrate and Invertebrate
Muscles
Ch. Gerday (With 4 Figures) . . . . . . . . . . . . . . . . . . . . . . . . . . .. 23
The Rat Parvalbumin Gene
M.W. Berchtold (With 3 Figures) 40
Calcium Binding to Troponin C and the Regulation of
Muscle Contraction: a Comparative Approach
W. Wnuk (With 2 Figures). . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 44
Caldesmon and Ca2+ Regulation in Smooth Muscles
S. Marston, W. Lehman, C. Moody, K. Pritchard, and C. Smith
(With 10 Figures) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 69
Thin Filament-Linked Regulation in Vertebrate Smooth Muscle
M.P. Walsh, G.C. Scott-Woo, M.S. Lim, C. Sutherland, and Ph.K. Ngai
(With 7 Figures) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 82
Calcium Binding Proteins in Non-Muscle Tissues
Parvalbumin in Non-Muscle Cells
C.W. Heizmann. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 93
S100 Proteins: Structure and Calcium Binding Properties
J. Baudier (With 4 Figures) . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 102
VIII Contents
Mechanisms of Action of the S100 Family of Calcium Modulated
Proteins
LJ. Van Eldik and D.B. Zimmer (With 1 Figure). . . . . . . . . . . . . .. 114
Comparative Studies on Oncomodulin
J.P. MacManus, L.M. Brewer, and M.F. Gillen (With 4 Figures). . . . .. 128
Calmodulin in the Regulation of Cellular Activity
Cation Binding to Calmodulin and Relation to Function
J.A. Cox, M. Comte, A. Mamar-Bachi, M. Milos, and J.-J. Schaer
(With 12 Figures) ................................... 141
Regulatory Properties of Bovine Brain Calmodulin-Dependent
Phosphatase
W.Y. Cheung . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 163
Calmodulin and Calmodulin Binding Proteins During Differentiation
of Human Intestinal Brush Borders
C. Rochette-Egly, B. Lacroix, M. Kedinger, and K. Haffen
(With 9 Figures) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 179
Site-Specific Mutagenesis and Protein Engineering Approach to
the Molecular Mechanism of Calcium Signal Transduction by
Calmodulin
J. Haiech and D.M. Watterson. . . . . . . . . . . . . . . . . . . . . . . . . .. 191
The Role of Calcium in the Regulation of Plant Cellular Metabolism
D. Marme. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 201
Calcium in Different Biological Systems
Calcium Homeostasis in Exocrine Secretory Cells
R.M. Case, T.-A. Ansah, S. Dho, A. Miziniak, and L. Wilson
(With 6 Figures) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 211
The Regulation of the Ca-Pumping Activity of Cardiac Sarcoplasmic
Reticulum by Calmodulin
M. Chiesi and J. Gasser (With 5 Figures). . . . . . . . . . . . . . . . . . . .. 220
Calcium Binding and Structural Characteristics of Muscle
Calsequestrins
S. Salvatori, M. Bravin, and A. Margreth (With 4 Figures) . . . . . . . .. 228
Contents IX
Studies on Voltage-Gated Calcium Channels by Means of
Fluorescent [Ca2+] Indicators
J. Meldolesi, A. Malgaroli, A. Pandiella, A. Ambrosini, L.M. Vicentini,
D. Milani, F. DiVirgilio, and T. Pozzan (With 2 Figures) . . . . . . . . .. 236
Calcium Entry Blockers
M. Wibo and T. Godfraind (With 6 Figures) . . . . . . . . . . . . . . . . .. 243
Subject Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 255
Contributors
You will find the addresses at the beginning of the respective contribution
Ambrosini, A. 236 Mamar-Bachi, A. 141
Ansah, T.-A. 211 Margreth, A. 228
Baudier, J. 102 Marme, D. 201
Berchtold, M.W. 40 Marston, S. 69
Bravin, M. 228 Meldolesi, J. 236
Brewer, L.M. 128 Milani, D. 236
Case, R.M. 211 Milos, M. 141
Cheung, W.Y. 163 Miziniak, A. 211
Chiesi, M. 220 Moody,C. 69
Cockcroft, S. 3 Ngai, Ph.K. 82
Comte, M. 141 Pandiella, A. 236
Cox, J.A. 141 Pozzan, T. 236
Dho, S. 211 Pritchard, K. 69
DiVirgilio, F. 236 Rochette-Egly, C. 179
Gasser, J. 220 Salvatori, S. 228
Gerday, Ch. 23 Schaer, J.-J. 141
Gillen, M.F. 128 Scott-Woo, G.C. 82
Godfraind, T. 243 Smith, C. 69
Haffen, K. 179 Sutherland, C. 82
Haiech,J. 191 Van Eldik, L.J. 114
Heizmann, C.W. 93 Vicentini, L.M. 236
Kedinger, M. 179 Walsh, M.P. 82
Lacroix, B. 179 Watterson, D.M. 191
Lehman, W. 69 Wibo,M. 243
Lim,M.S. 82 Wilson, L. 211
MacManus J.P. 128 Wnuk, W. 44
Malgaroli, A. 236 Zimmer, D.B. 114
