Table Of ContentContributors to Volume 291
elcitrA srebmun era ni sesehtnerap gniwollof the seman of .srotubirtnoc
snoitailiffA listed era .tnerruc
ANDREW C. ALLAN (26), Department of Plant CHUNG-Yu CHANG (7), Worcester Founda-
Genetics, Weizmann Institute of Science, tion for Biomedical Research, Shrewsbury,
Rehovot, Israel 00167 Massachusetts 54510
HPOTSIRHC ALLIN (12), Lehrstuhl far Bio- R. S. CHrrrocK (13, 27), School of Biochemis-
physik, Fakultiit Biologie, 44780 Bochum, try, University of Birmingham, Birmingham
Germany B15 2TT, United Kingdom
E. BAMBERG (17), Max Planck Institute for JODY A. DANTZIG (18), Pennsylvania Muscle
Biophysics, D-60596 Frankfurt, Germany,
Institute, University of Pennsylvania School
and Johann Wolfgang Goethe-Universiti~t
of Medicine, Philadelphia, Pennsylvania
Frankfurt am Main Biozentrum, D-60439
40191
Frankfurt am Main, Germany
CLAUDIA DZEJA (23), Institut far Biologische
HAGAN BAYLEY (7), Department of Medical
Informationsverarbeitung, Forschungszen-
Biochemistry and Genetics, Texas A&M
trum Jiilich, D-52425 Jiilich, Germany
Health Science Center, College Station,
Texas 34877 DAVID EPEL (16), Department of Biological
MICHAEL H. BEALE (26), IA CR-Long Ashton Sciences, Hopkins Marine Station, Stanford
Research Station, Department of Agricul- University, Pacific Grove, California 05939
tural Sciences, University of Bristol, Bristol FREDRIC S. FAY* (5), Department of Physiol-
BS18 9AF, United Kingdom ogy, University of Massachusetts, Worces-
JORGEN BENDIG (23), Institut far Organische ter, Massachusetts 50610
und Bioorganische Chemie, Humboldt- K. FENDLER (17), Max Planck Institute for
Universitiit Berlin, D-10115 Berlin, Biophysics, D-60596 Frankfurt, Germany
Germany
NAHPETS FRINGS (23), Institutfar Biologische
EDWARD B. BROWN (20), Department of
Informationsverarbeitung, Forschungszen-
Physics, Cornell University, Ithaca, New
trum Jiilich, D-52425 Jiilich, Germany
York 35841
CHmSTOPH RETSEMRUB (14), Abteilung Phy- IHSEKAT IHSOKANUF (19), Department of Cell
Biology and Anatomy, Graduate School of
sikalische Biochemie, Max Planck Institute
Medicine, University of Tokyo, Hongo,
for Molecular Physiology, 44139 Dort-
Tokyo 113, Japan
mund, Germany
NICK SARAMALLAC (21), Laboratory of Cellu- IKAIHSOT FURUTA (3), Department of Biomo-
lar and Molecular Neurobiology, Depart- lecular Science, Faculty of Science, Toho
ment of Psychobiology, University of Cali- University, Chiba 274, Japan
fornia, Irvine, California 92697 DANIELA GABRIEL (6), Max Planck Insti-
BARRY K. CARPENTER (2), Department of tute for Biochemistry, 82152 Martinsried,
Chemistry, Section of Biochemistry, Molec- Germany
ular and Cell Biology, Cornell University,
KYLE R. GEE (2, 4, 22), Molecular Probes,
Ithaca, New York 35841 Inc., Eugene, Oregon 20479
VALENTIN SUPEC (12), Lehrstuhl far Bio-
physik, Fakulti~t Biologie, 44780 Bochum,
Germany *Deceased
ix
X CONTRIBUTORS TO VOLUME 291
KLAUS GERWERT (12), Lehrstuhl far Bio- MITSUO IKEBE (5), Department of Physiology,
physik, Fakultgit Biologie, 44780 Bochum, University of Massachusetts, Worcester,
Germany Massachusetts 50610
RICHARD S. GIVENS (1), Department of Chem- MICHIKO IWAMURA (3), Department of Bio-
istry, University of Kansas, Lawrence, Kan- molecular Science, Faculty of Science, Toho
sas 54066 University, Chiba 274, Japan
MAURICE GOELDNER (15), Laboratoire de ANDREAS H. JUNG (1), Department of Chem-
chimie Bioorganique, Facultd de Pharma-
istry, University of Kansas, Lawrence, Kan-
cie, Universitd Louis Pasteur Strasbourg,
sas 54066
BP 10476F-42 lllkirch Cedex, France
JOSEPH P. Y. KAO (24), Department of Physi-
YALE E. GOLDMAN (18), Pennsylvania Muscle
ology, University of Maryland School of
Institute, University of Pennsylvania School
Medicine, and Medical Biotechnology Cen-
of Medicine, Philadelphia, Pennsylvania
ter, University of Maryland Biotechnology
40191
Institute, Baltimore, Maryland 10212
H. MAURICE GOODMAN, Department of Phys-
U. BENJAMIN KAUPP (23), Institut fur Bio-
iology, University of Massachusetts Medical
logische Informationsverarbeitung, For-
School, Worcester, Massachusetts 50610
schungszentrum Jiilich, D-52425 Jiilich,
ROGER S. GOODY (14), Abteilung Physikal-
Germany
ische Biochemie, Max Planck Institute for
Molecular Physiology, 44139 Dortmund, EDYTA KRZYMAlqSKA-OLEJNIK (8), Am-
Germany bergen, Inc., Boston, Massachusetts 51220
CHRISTOF GREWER (25), Section of Biochem- HON CHEUNG LEE (22), Department of Physi-
istry, Molecular and Cell Biology, Cornell ology, University of Minnesota, Minneapo-
University, Ithaca, New York 35841 lis, Minnesota 55455
VOLKER HAGEN (23), Forschungsinstitut fiir A. MALLAVARAPU (4), Biochemistry Depart-
Molekulare Pharmakologie, D-10315 Ber- ment, University of California, San Fran-
lin, Germany cisco, California 34149
K. HARTUNG (17), Max Planck Institute for MICHAEL MARGULIS (24), Department of
Biophysics, D-60596 Frankfurt, Germany Neurology, University of Maryland School
MANFRED HEIDECKER (6), Biomolecular and of Medicine, Baltimore, Maryland 10212
Cellular Dynamics Group, Max Planck In-
GERARD MARRIOTT (6, 9), Max Planck Insti-
stitute for Biochemistry, 82152 Martins-
tute for Biochemistry, 82152 Martinsried,
ried, Germany
Germany
GEORGE P. HESS (2, 25), Section of Biochem-
JAMES A. MCCRAY (10), Department of Phys-
istry, Molecular and Cell Biology, Cornell
ics, Drexel University, Philadelphia, Penn-
University, Ithaca, New York 35841
sylvania ,40191 and Division of Neurophys-
HIDEO HIGUCH1 (18), Department of Metal-
iology, National Institute for Medical
lurgy, Faculty of Engineering, Tohoku Uni-
Research, London NW7 1AA, England
versity, Sendai ,77-089 Japan
R. A. MELDRUM (27), School of Biochemistry,
NOBUTAKA HIROKAWA (19), Department of
University of Birmingham, Birmingham
Cell Biology and Anatomy, Graduate
B15 2TT, United Kingdom
School of Medicine, University of Tokyo,
Hongo, Tokyo 113, Japan W. TODD MILLER (7), Department of Physiol-
ROBERT E. HOESCH (24), Medical Biotechnol- ogy and Biophysics, SUNY Health Sciences
ogy Center, University of Maryland Bio- Center, Stony Brook, New York 11794
technology Institute, and University of T. J. MITCHlSON (4), Department of Cell Biol-
Maryland School of Medicine, Baltimore, ogy, Harvard Medical School, Boston, Mas-
Maryland 10212 sachusetts 51120
CONTRIBUTORS TO VOLUME 192 xi
G. NAGEL (17), Johann Wolfgang Goethe- lecular Physiology, 44139 Dortmund,
tatisrevinU Frankfurt am Main Biozentrum, ynamreG
93406-D Frankfurt am Main, Germany R. SREEKUMAR (5), Department of Physiol-
BRETT NIBLACK (7), Worcester Foundation ogy, University of Wisconsin, Madison,
for Biomedical ,hcraeseR ,yrubswerhS Mas- nisnocsiW 60735
sttesuhcas 54510 ROBERT R. SWEZEY (16), Department of
JERZY OLEJNIK (8), Department of Physics scitenikocamrahP and Metabolism, Sha-
and Molecular Biophysics Laboratory, man ,slacituecamrahP Inc., South naS -narF
Boston ,ytisrevinU Boston, sttesuhcassaM ,ocsic California 08049
,51220 and AmberGen, Inc., Boston, Mas- CHA-MIN TANG (24), Department of Neurol-
sttesuhcas 51220 ogy, ytisrevinU of dnalyraM School of -deM
,enici Baltimore, Maryland 10212
SENNAHOJ OTFL (6, 9), Max Planck Institute
for Biochemistry, 82152 ,deirsnitraM J. A. THERIOT (4), Whitehead ,etutitsnI Cam-
ynamreG ,egdirb sttesuhcassaM 24120
PENG PAN (7), Boehringer Ingelheim Phar- ANTHONY J. TREWAVAS (26), Institute of lleC
,slacituecam Inc., Ridgefield, Connecticut and Molecular Biology, ytisrevinU of Edin-
77860 burgh, Edinburgh EH3 9JH, United
Kingdom
CHAN-Ho PARK (1), Research and Develop-
AGNES TROULLIER (12), Lehrstuhl far Bio-
ment ,retneC Hansol Institute of ecneicS and
physik, Fakultiit Biologic, 44780 Bochum,
Technology, Nomyangju-Si, Kyung -iK
ynamreG
,oD Korea
CAROLA ULBRICH (12), Lehrstuhl far Bio-
IAN PARKER (21), Laboratory of ralulleC and
physik, Fakultiit Biologie, 44780 Bochum,
Molecular Neurobiology, Department of
ynamreG
Psychobiology, University of ,ainrofilaC
Irvine, California 79629 JEEEERY W. WALKER (5), Department of
,ygoloisyhP ytisrevinU of Wisconsin School
LING PENG (15), Laboratoire ed chimie Bioor-
of ,enicideM Madison, Wisconsin 60735
,euqinag ~tlucaF ed Pharmacie, ~tisrevinU
JANE L. WARD (26), IACR-Long Ashton Re-
Louis Pasteur Strasbourg, BP 10476F-42
hcraes Station, Department of Agricultural
lllkirch ,xedeC ecnarF
,secneicS ytisrevinU of ,lotsirB BristoI BS18
GERT RAPP (11), European Molecular Biol-
9AF, United Kingdom
ogy Laboratory, Outstation at DESY,
DAVID ,WAHSRAW Department of raluceloM
30622-D Hamburg, Germany
Physiology and Biophysics, University of
FRANCIS M. RossI (24), Medical Biotechnol- Vermont Medical School, Burlington, Ver-
ogy Center, University of Maryland Bio- mont 50450
technology Institute, Baltimore, Maryland
WATT W. WEBB (20), Department of Applied
10212
and Engineering Physics, Cornell -revinU
KENNETH J. ROTHSCHILD (8), Department of ,ytis Ithaca, New York 35841
,scisyhP Boston ,ytisrevinU Boston, -assaM
JORG F. W. WEBER (1), Department of -mehC
sttesuhc 51220
,yrtsi ytisrevinU of ,sasnaK Lawrence, Kan-
K. E. SAWIN (4), ICRF, Lincolns Inn, London sas 54066
2CW 3PX, England C. W. WHARTON (13, 27), School of Biochem-
AXLE SCHEIDIG (14), Abteilung ehcsilakisyhP istry, University of Birmingham, Bir-
Biochemie, Max Planck Institute for Mo- mingham B15 2TT, United Kingdom
Preface
Caged compounds are biomolecules whose activity or function is
inactivated by chemical modification with a photolabile group. Excitation
of a caged compound with (near-ultraviolet) light results in specific
cleavage of a bond within the molecule that rapidly liberates the functional
biomolecule of interest. One main advantage of this light-directed pertur-
bation technique centers around its ability to overcome the mixing and
diffusion time limits of fast-flow methods. This is achieved by precharging
the biological sample with a caged substrate, ligand, or protein conjugate,
followed by light-directed activation of the caged compound which triggers
the specific reaction under investigation within the excitation volume.
In addition, concentration jumps mediated by light-directed activation
of caged compounds can be conducted without physically disturbing the
preparation. Caged groups described in this volume generate active
biomolecules within nanoseconds of absorbing light and, furthermore,
by using two-photon excitation of the caged compound, the concentration
jump can be confined to a volume having a diameter of approximately
250 nm with little or no irradiation-based damage to the preparation.
Light-directed activation of caged compounds and rapid monitoring of
the ensuing reaction using photomultiplier or imaging-based techniques
have been used to understand the molecular mechanism of several
biochemical reactions and processes in vitro and in vivo. This volume
also describes the application of caged compounds to studies that aim
to understand the molecular basis of neurotransmission, muscle contrac-
tion, and ion channel function.
Topics covered in this book include (a) an up-to-date account of the
synthetic chemistry and photochemistry of traditional and newer genera-
tions of caged groups, (b) a description of new reagents and methods to
prepare monofunctional and bifunctional complexes of a diverse array of
caged substrates, ligands, and polypeptides, (c) a state-of-the-art practical
and theoretical description of lamp- and laser-based excitation sources for
light-directed activation of caged compounds in macro- and microscopic
samples, (d) the measurement and interpretation of spectroscopic signals
derived from light-directed activation of caged compounds to monitor the
kinetics of the reaction being studied, and (e) the application of light-
directed activation of caged compounds and caged polypeptides to chemical
relaxation studies that aim to understand the molecular mechanism of
protein-catalyzed reactions in a diverse array of complex molecular environ-
ments.
xiii
xiv ECAFERP
The primary objective of this volume is to provide newcomers to the
field with a central source of valuable practical and theoretical information
on caged compounds that will allow them to use light-directed activation
of caged compounds to address molecular mechanisms of biochemical reac-
tions or processes within biological samples. It provides the investigator
with the most up-to-date and comprehensive account of caged compounds
that should aid in the design of experiments to compare and interpret data
obtained from kinetic measurements of specific reactions triggered by light-
directed activation of caged biomolecules.
This volume is dedicated to the memory of Professor Fredric S. Fay.
GERARD MARRIOTT
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV II. Preparation and Assay of Enzymes
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV III. Preparation and Assay of Substrates
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O, KAPLAN
EMULOV IV. Special Techniques for the Enzymologist
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV V. Preparation and Assay of Enzymes
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV VI. Preparation and Assay of Enzymes (Continued)
Preparation and Assay of Substrates
Special Techniques
Edited yb SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV VII. Cumulative Subject Index
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPEAN
EMULOV VIII. Complex Carbohydrates
Edited by ELIZABETH F. NEUFELD DNA VICTOR GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited by WILLIS A. WOOD
EMULOV X. Oxidation and Phosphorylation
Edited by RONALD W. KOORBATSE DNA MAYNARD E. NAMLLUP
EMULOV XI. Enzyme Structure
Edited by C. H. W. HIRS
EMULOV XII. Nucleic Acids (Parts A and B)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XIII. Citric Acid Cycle
Edited by J. M. NIETSNEWOL
VOt,UME XIV. Lipids
Edited by J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited yb DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
Edited by NITSUK HTENNEK
XV
XVi METHODS IN ENZYMOLOGY
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited by TREBREH ROBAT DNA AILEC ETIHW ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited by DLANOD B. KCIMRoCcM DNA LEUMEL D. THGIRW
EMULOV XIX. Proteolytic Enzymes
by Edited EDURTREG E. NNAMLREP DNA OLZSAL DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited by MAILLIW B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited yb YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by .C H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
by Edited ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXII. Biomembranes (Part B)
by Edited YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by AHTRAM G. SINNED DNA DRAWDE A. SINNED
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited by MAILLIW B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XXXV. Lipids (Part B)
Edited by NHOJ .M NIETSNEWOL
SDOHTEM IN YGOLOMYZNE xvii
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited by JOEL G. HARDMAN DNA BERT W. O'MALLEY
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and Organ
Systems)
Edited by JOEL G. HARDMAN DNA BERT W. O'MALLEY
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited by W. A. WOOD
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited by W. A. WOOD
EMULOV XLIII. Antibiotics
Edited by JOHN H. HASH
EMULOV XLIV. Immobilized Enzymes
Edited by KLAUS HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited by LASZLO DNAROL
EMULOV XLVI. Affinity Labeling
Edited by WILLIAM B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XLVII. Enzyme Structure (Part E)
Edited by C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV XLVIII. Enzyme Structure (Part F)
Edited by C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV XLIX. Enzyme Structure (Part G)
Edited by C. H. W. HIRS DNA SERGE N. FFEHSAMIT
EMULOV L. Complex Carbohydrates (Part C)
Edited by VICTOR GRUBSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited by PATRICIA A. HOFFEE DNA MARY ELLEN SENOJ
EMULOV LII. Biomembranes (Part C: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA LESTER REKCAP
EMULOV LIII. Biomembranes (Part D: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA LESTER REKCAP
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA LESTER REKCAP
xviii METHODS IN ENZYMOLOGY
EMULOV LV. Biomembranes (Part F: Bioenergetics)
Edited yb SIDNEY FLEISCHER AND LESTER PACKER
EMULOV LVI. Biomembranes (Part "G Bioenergetics)
Edited by SIDNEY FLEISCHER AND LESTER PACKER
EMULOV LVII. Bioluminescence and Chemiluminescence
Edited by ENELRAM A. DELuCA
EMULOV LVIII. Cell Culture
Edited by MAILLIW B. YBOKAJ DNA ARI NATSAP
EMULOV LIX. Nucleic Acids and Protein Synthesis (Part G)
Edited yb KIVIE MOLDAVE AND LAWRENCE GROSSMAN
EMULOV LX. Nucleic Acids and Protein Synthesis (Part H)
Edited by EIV~K MOLDAVE AND LAWRENCE GROSSMAN
EMULOV .16 Enzyme Structure (Part H)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV .26 Vitamins and Coenzymes (Part D)
Edited by DONALD B. McCORMICK AND LEMUEL D. WRIGHT
EMULOV .36 Enzyme Kinetics and Mechanism (Part A: Initial Rate and Inhibitor
Methods)
Edited by DANIEL L. PURICH
EMULOV .46 Enzyme Kinetics and Mechanism (Part "B Isotopic Probes and Com-
plex Enzyme Systems)
Edited by DANIEL L. PURICH
EMULOV .56 Nucleic Acids (Part I)
Edited by LAWRENCE GROSSMAN AND KIVIE MOLDAVE
EMULOV .66 Vitamins and Coenzymes (Part E)
Edited by DONALD B. McCORMICK AND LEMUEL D. WRIGHT
EMULOV .76 Vitamins and Coenzymes (Part F)
Edited by DONALD B. McCoRMICK AND LEMUEL D. WRIGHT
VOLUME 68. Recombinant DNA
Edited yb YAR Wu
EMULOV .96 Photosynthesis and Nitrogen Fixation (Part C)
Edited by YNOHTNA NAS ORTEIP
EMULOV .07 Immunochemical Techniques (Part A)
Edited by HELEN VAN VUNAKIS AND JOHN J. LANGONE
EMULOV .17 Lipids (Part C)
Edited by JOHN M. NIETSNEWOL
EMULOV .27 Lipids (Part D)
Edited by JOHN M. LOWENSTEIN
METHODS IN ENZYMOLOGY xix
EMULOV .37 Immunochemical Techniques (Part B)
Edited by JOHN J. ENOGNAL DNA HELEN NAV SIKANUV
EMULOV .47 Immunochemical Techniques (Part C)
Edited by JOHN J. ENOGNAL DNA HELEN NAV SIKANUV
EMULOV .57 Cumulative Subject Index Volumes XXXI, XXXII, XXXIV-LX
Edited by DRAWDE A. SINNED DNA AHTRAM G. SINNED
EMULOV .67 Hemoglobins
Edited by ODLARE ,ININOTNA LUIGI ,IDRANREB-ISSOR DNA EMILIA ENOCNAIHC
EMULOV .77 Detoxication and Drug Metabolism
Edited by MAILLIW B. YBOKAJ
EMULOV .87 Interferons (Part A)
Edited by YENDIS AKTSEP
EMULOV .97 Interferons (Part B)
Edited by YENDIS AKTSEP
EMULOV .08 Proteolytic Enzymes (Part C)
Edited by OLZSAL DNAROL
EMULOV .18 Biomembranes (Part H: Visual Pigments and Purple Mem-
branes, I)
Edited by RETSEL REKCAP
EMULOV .28 Structural and Contractile Proteins (Part A: Extracellular Matrix)
Edited by LEON W. CUNNINGHAM DNA DIXIE W. FREDERIKSEN
EMULOV .38 Complex Carbohydrates (Part D)
Edited by ROTCIV GRUBSNIG
EMULOV .48 Immunochemical Techniques (Part D: Selected Immunoassays)
Edited by JOHN J. LANGONE DNA HELEN VAN VUNAKIS
EMULOV .58 Structural and Contractile Proteins (Part B: The Contractile Appara-
tus and the Cytoskeleton)
Edited by DIXIE W. NESKIREDERF DNA LEON W. MAHGNINNUC
EMULOV .68 Prostaglandins and Arachidonate Metabolites
Edited by WILLIAM E. M. LANDS DNA WILLIAM L. SMITH
EMULOV .78 Enzyme Kinetics and Mechanism (Part "C Intermediates, Stereo-
chemistry, and Rate Studies)
Edited by DANIEL L. PURICH
EMULOV .88 Biomembranes (Part I: Visual Pigments and Purple Mem-
branes, II)
Edited by RETSEL REKCAP
EMULOV .98 Carbohydrate Metabolism (Part D)
Edited by WILLIS A. WOOD
EMULOV .09 Carbohydrate Metabolism (Part E)
Edited yb WILLIS A. WOOD
