By the name of Allah Sudan University of Science and Technology College of Science Scientific

35 Pages·2003·1.42 MB·Arabic

By the name of Allah
Sudan University of Science and Technology
College of Science
Scientific Laboratories Department-Chemistry
A complementary research for BA (HONS):
Development and Validation of Spectrophotometric Method for
Determination of Penicillamine (PA) in Pharmaceutical Formulation Using
4-Chloro-7-Nitrobenzo-2-Oxa-1, 3-Diazol (NBD-Cl)

By:
 Maaza Alnazeer Babikir.
 Mariam Abd-almonem Abd-allah.
 Mohammed Hamid Ahmed.

Supervisor:
Amira Anwar Babikir
2015-1437
ةيلآا

﴾ املْ عِ يِندْ زِ يِبرَ لْ ُقو﴿

هط ةروس
)111 ةيلآا(
أ
Dedication
To my parents.
Who inspired me with faith in Allah, love and respect
towards all people
and sincerity in work.
To my Friends
for their sacrifice and encouragement.

ب
Acknowledgment
Thanks to Allah
First I would like to express my thanks to my supervisor Amira Anwar
Babikir, for
her interest in the research project and guidance and help during the
research.
My thanks extend to the technicians of Univ ersity of Khartoum laboratories
(shambat).
ج
:ثحبلا صلختسم
ةقيرطلا هذه موقت .يئاود بكرمك )D-Penicillamine( ديدحتل ةساسحو ةطيسب ةيفيط ةقيرط حارتقإ مت
لوزايد-1,1-اسكوا-2-وزنبورتين-7-ورولك-1 عم )PA( لعافتل يناوجرلأا-ينبلا ةفاضلإا جتان ساسأ ىلع
,)pH=10.5( يدعاق طسو يف (NBD-Cl))4-chloro-7-nitrobenzo-2-oxa-1, 3-diazole(
ةقلاع يف ةقيرطلا تناك ,ىلثملا لعافتلا فورظ تحت .465nm ( ) ىوصق ةيصاصتمإ ىدم يف
max
لكش يف PA زيكرت داجيلإ حاجنب ةقيرطلا قيبطت مت .1-15µg زيكرت ىدم يف زيكرتلا عم ةيطخ
ةيكيرملأا ةدحتملا تايلاولا روتسدل ةيمسرلا ةقيرطلا جئاتن عم ةبراقتم جئاتنلا تناكو .ةيئاودلا تاعرج
.ةدوجلا طبض تاربتخم يف )PA( نيملاسينبلل ينيتورلا ليلحتلل ةديفم ةقيرطلا هذه .ةيودلأل
د
Abstract:
A sensitive and simple spectrophotometric method has been proposed for the
determination of D-Penicillamine (PA) in pharmaceutical formulation. The proposed
method is based on the reaction between the PA and 4-chloro-7-nitrobenzo-2-oxa-1, 3-
diazole (NBD-Cl) at alkaline medium (pH=10.5) to form deep brown-purple adduct,
exhibiting maximum absorption ( ) at 465nm. Under optimized reaction condition, the
max
method was linear in the concentration rage 1-15µgmL1. The method was applied
successfully to the determination if PA in pharmaceutical dosage form. The results were
in a good agreement with the official USP method. The method is useful for routine
analysis of PA in quality control laboratories.

ه
Contents:

Subject Subject Page
number number
i. ةيلآا أ
ii. Dedication ب
iii. Acknowledgment ج
iv. Abstract د
Chapter One
1-1 Penicillamine 1
1-1-1 Systematic name 1
1-1-2 Penicillamine definition 1
1-1-3 Clinical data 2
1-1-4 Chemical data 2
1-1-5 prescription drug 2
1-1-6 Uses of Penicillamine 3
1-1-7 Medical Uses of Penicillamine 4
1-1-8 Adverse effects of Penicillamine 4
1-1-9 Penicillamine side effects 5
1-1-10 Penicillamine Pregnancy 6
Warnings
1-2 NBD-Cl 8
1-2-1 Properties of NBD-Cl 8
1-2-2 Applications of NBD-Cl 9
1-2-3 General description of NBD-Cl 9
1-3 UV-1800 10
Chapter Two
2-1 Apparatus 14
2-2 Reagent and Solution 14
2-3 Procedure 14
2-3-1 Calibration Curve 14
2-3-2 Determination of D-Pain dosage 15
form
Chapter Three
3-1 results 16
3-1-1 Buffer solution absorption 16
3-1-2 Absorption of reagent 17
3-1-3 Absorption of Penicillamine 18
3-1-4 Parameters for the performance 19
of the proposed method
3-2 Conclusion 20
3-3 Discussion 21
References 22
Chapter one
Theoretical section