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About Blue White Screening of Bacterial Colonies Using X-Gal and IPTG Plates
This document provides a protocol for blue-white screening of bacterial colonies using X-Gal and IPTG plates. It describes how β-galactosidase enzyme activity allows detection of recombinant bacteria by complementing disrupted lacZ genes. The protocol involves preparing X-Gal and IPTG solutions, then screening transformed bacterial colonies on agar plates containing these to distinguish blue non-recombinant colonies from white recombinant colonies.
Detailed Information
| Author: | ['Raja Tripathi'] |
|---|---|
| Publication Year: | 2020 |
| Pages: | 4 |
| Language: | English |
| Format: | |
| Price: | FREE |
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