Table Of ContentProceedings in Life Sciences
Biosynthesis, Metabolism
and Mode of Action
of Invertebrate Hormones
Edited by
1. Hoffmann and M. Porchet
With 154 Figures
Springer-Verlag
Berlin Heidelberg New York Tokyo 1984
Professor Dr. JULES HOFFMANN
Laboratoire de Biologie Generale
de I'Universite L. Pasteur et
Unite de Recherche Associee au CNRS
«Endocrinologie et Immunologie des Insectes»
12, Rue de l'Universite
67000 Strasbourg, France
Professor MAURICE PORCHET
Universite des Sciences et Techniques
de Lille
59655 Villeneuve D' Asq, France
ISBN-13: 978-3-642-69924-5 e-ISBN-13: 978-3-642-69922-1
DOl: 10.1 007/978-3-642-69922-1
Library of Congress Cataloging in Publication Data. Main entry under title: Biosynthesis,
metabolism, and mode of action of invertebrate hormones. (Proceedings in life sciences).
1. Invertebrates - Physiology. 2. Hormones. I. Hoffmann, J. (Jules), 1941-. II. Porchet, M.
(Maurice), 1943-. III. Series. QL364.B56. 1984.592'.01927.84-13959.
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2131/3130-543210
Preface
The neuroendocrine control of reproduction and development of inver
tebrates has a long tradition as an important area of research in France.
The reader of this volume is certainly familiar with the significant con
tributions to this field made by such outstanding scientific personalities
as J ean-J acques Bounhiol, Jean Panouse, Bernard Possompes, Pierre and
Line Joly, Helene Charniaux-Cotton,Maurice Durchon, Manfred Gabe,
Guy Echalier, Marie Raabe, and others.
It is therefore not surprising that the Centre National de la Recherche
Scientifique (CNRS) sponsored, in 1975 and 1983, two major inter
national meetings devoted to this subject. The organizers of the 1975
meeting, which was held in Lille, decided to concentrate on the bio
synthesis, metabolism, and mode of action of the invertebrate hor
mones. To some extent, Professors Durchon and J oly wanted to convey
the message that they felt that the period of classical invertebrate endo
crinology had come to an end and that traditional approaches were be
ing replaced by biochemical analyses. How right they were is illustrated
by the present volume. Today biochemistry, molecular biology, and
analytical chemistry are tools of the invertebrate endocrinologist, who
now starts his morning work by homogenizing his worm, mollusc,
insect ... and by extracting DNA, steroids, or peptides.
The aim of the second meeting, which brought some 200 scientists
together in Strasbourg during one sunny August week in 1983, was
both to summarize the state of the art and to outline the trends of
future research in the field of invertebrate hormones.
The progress made between the two meetings is indeed considerable.
The structures of half a dozen peptide hormones have been elucidated
and many others are close to identification, whereas in 1975 the amino
acid sequence of only one invertebrate peptide hormone, the Red Pig
ment Concentrating Hormone of prawns, was known. The list of juve
nile hormones has grown from 3 to 5, and that of Zooecdysteroids has
increased at least five-fold and now exceeds 30. Radioimmunoassays,
in their infancy at Lille, have since allowed the recording of minute
fluctuations of ecdysteroid titers and, more recently, of juvenile hor
mone titers; high pressure liquid chromatography has allowed the ac
curate separation of closely related hormone precursors or metabolites,
which have then been identified by impressive batteries of spectroscopic
VI Preface
techniques. Although the Lille meeting listed amongst its objectives the
study of the mode of action of invertebrate hormones, few precise
results were available, whereas 8 years later a long series of lectures
gave significant information on ecdysteroid and juvenile hormone re
ceptors and on the mode of action of these hormones at the genome
level.
The Editors have asked the various authors to survey progress in
their respective fields and to highlight the perspectives and prospects.
It is hoped that the reader will find this book useful and that it will
stimulate young scientists to enter the rapidly expanding field of re
search on invertebrate hormones.
JULES HOFFMANN
Contents
Part I Peptide Hormones
Introduction
J. Hoffmann 2
I.l Hormonal Control of Regeneration in Hydra
H.C. Schaller, S. Hoffmeister, and H. Bodenmi.iller 5
1.2 Peptidic Hormones in Annelids
M. Durchon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 10
1.3 Recent Progress in Endocrinology of Molluscs
J. J oosse (With 1 Figure) .. . . . . . . . . . . . . . . . . . . . .. 19
1.4 The Structure of the Commands for a Neuropeptide
Mediated Behavior, Egg-Laying, in an Opisthobranch
Mollusc
F. Strumwasser ............................. 36
1.5 Studies on Release Activities of the Neurosecretory
Caudo-Dorsal Cells of Lymnaea stagnalis
W.P.M. Geraerts, A. ter Maat, and Th. M. Hogenes
(With 4 Figures) . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 44
1.6 Roles of Calcium and cAMP in Biosynthesis and Release
of the Ovulation Hormone of the Freshwater Snail
Lymnaea stagnalis
E.w. Roubos, P. Burna, and A.M.H. van de Ven
(With 2 Figures) . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 51
1.7 The Egg-Laying Behavior of the Pond Snail:
Electrophysiological Aspects
A. ter Maat and R.F. Jansen (With 4 Figures) . . . . . . . . .. 57
1.8 Prothoracicotropic Hormone of Bombyx mori
H. Ishizaki and A. Suzuki (With 11 Figures) . . . . . . . . . .. 63
VIII Contents
1.9 Neurohonnonal Regulation of Molting and Metamorphosis
in the Tobacco Hornworm, Manduca sexta
W.E. Bollenbacher, N.A. Granger, W.A. Smith, and
L.1. Gilbert .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 78
1.10 Isolation of an Aliatotropic Factor in Locusta migratoria
and Its Effect on Corpus Allatum Activity in Vitro
H.J. Ferenz (With 2 Figures) ..................... 92
1.11 Neurosecretion in Insects: Multisynthesis, Mode of Action,
and Multiregulation
A. Girardie, J. Girardie, J. Proux, C. Remy, and
J. Vieillemaringe (With 1 Figure) . . . . . . . . . . . . . . . . .. 97
1.12 Neuropeptides of Vertebrate Type in the Blowfly Calliphora
A. Thorpe and H. Duve (With 3 Figures) ............. 106
1.13 Peptides from the Corpora cardiac a and CNS of Locusts
W. Mordue and P.J. Morgan (With 2 Figures) .......... 118
1.14 Adipokinetic Hormones in Locusts
GJ. Goldsworthy and C.H. Wheeler (With 3 Figures) ..... 126
1.15 Ecdysteroids Regulate the Release and Action of
Ec1osion Honnone in the Moth Manduca sexta
J.W .. Truman (With 3 Figures) .................... 136
1.16 Studies on Crustacean Eyestalk Neuropeptides by Use
of High Perfonnance Liquid Chromatography
R. Keller and G. Kegel (With 7 Figures) ............. 145
1.17 Present State of Knowledge on the Chemical Nature of the
Androgenic Honnone in Higher Crustaceans
P. Juchault, JJ. Legrand, and J. Maissiat ............. 155
Part II Ecdysteroids
Introduction
P. Karlson and J. Hoffmann ..................... 162
II.1 Ecdysteroids in Ovaries and Embryos of Locusta migratoria
M. Lagueux, J.A. Hoffmann, F. Goltzene, C. Kappler,
G. Tsoupras, C. Hetru, and B. Luu (With 5 Figures) ...... 168
11.2 Biosynthesis of Ovarian Ecdysteroid Phosphates and
Their Metabolic Fate During Embryogenesis in
Schistocerca gregaria
H.H. Rees and R.E. Isaac (With 2 Figures) ............ 181
Contents IX
11.3 Ecdysone Metabolism
R. Lafont and J. KooIman (With 7 Figures) ........... 196
I1A Ecdysone 20-Monooxygenases
G.F. Weirich, J.A. Svoboda, and M.1. Thompson
(With 2 Figures) ............................. 227
II.s Ecdysteroids and Integuments in Locusts
P. Porcheron, J.-P. Caruelle, J.-c. Baehr, and P. Cassier
(With 8 Figures) ............................. 234
11.6 Relationships Between Hormones and Epidermal
Cell Cycles During the Metamorphosis of Tenebrio moUtor
J. Delachambre, M.T. Besson, A. Quennedey, and
J.P. Delbecque (With 9 Figures) ................... 245
II. 7 On the Mode of Action of Ecdysteroids in Crustaceans
K.-D. Spindler, L. Dinan, and M. Londershausen
(With 4 Figures) ............................. 255
II.8 Hormonal Control of Sequential Gene Expression in
Insect Epidermis
L.M. Riddiford (With 2 Figures) .................. 265
I1.9 Ecd ysteroid Regulation of the Maj or Transcripts of
Drosophila melanogaster Larval Salivary Glands
and Fat Bodies
G. Richards and J.-A. Lepesant (With;3 Figures) ........ 273
I1.1O Ecdysteroid-Regulated Puffs and Genes in Drosophila
O. Pongs (With 1 Figure) ....................... 285
11.11 Pleiotropic Specific Responses Induced by Ecdysterone
in the Cultured Kc Cells of Drosophila melanogaster
J.-L. Couderc, M.-L. Sobrier, G. Giraud, D. Micard,
B. Dastugue, M. Ropp, J.-L. Becker, C. Maisonhllute,
F. Peronnet, A.-M. Courgeon, and M. Best-Belpomme
(With 2 Figures) ............................. 293
11.12 The Action of Ecdysone in Imaginal Discs and Kc Cells of
Drosophila melanogaster
L. Cherbas, J.W. Fristrom, and J.D. O'Connor
(With 2 Figures) ............................. 305
I1.l3 Ecdysteroids in Helminths
J. KooIman, J. Walter, and H. Zahner ............... 323
x Contents
11.14 Ecdysteroids in Schistosomes and Host-Parasite
Relationship
P. Nirde, G. Torpier, A. Capron, M. Delaage, and
M.L. de Reggi (With 4 Figures) ................... 331
11.15 The Occurrence and Possible Physiological Significance of
Ecdysteroids During Nematode and Cestode Development
H.H. Rees and A.H.W. Mendis .................... 338
11.16 Ecdysteroids in Annelids
M. Porchet, N. Gaillet, F. Sauber, M. Charlet, and
J .A. Hoffmann (With 1 Figure) ................... 346
Part III Juvenile Honnones
Introduction
L.1. Gilbert ., .............................. 350
III. 1 Radioimmunoassays of Juvenile Hormones. State of the
Methods and Recent Data on Validation
C. Strambi, A. Strambi, M. de Reggi, and M. Delaage
(With 5 Figures) ............................. 355
I1I.2 The Qualitative and Quantitative Determination of Juvenile
Hormones by Mass Spectrometry
B. Mauchamp, M. Zander, and R. Wolff (With 4 Figures) .. 363
111.3 Juvenile Hormones 0, I, and II Exist Only in Lepidoptera
D.A. Schooley, F.C. Baker, L.W. Tsai, C.A. Miller, and
G.C. Jamieson (With 2 Figures) ................... 373
IlIA Biochemical Aspects of Juvenile Hormone Biosynthesis
in Hemimetabola
G.E. Pratt (With 3 Figures) ...................... 384
I1I.5 Regulation of Juvenile Hormone Titer in the Adult Female
Cockroach Diploptera punctata
S.S. Tobe, B.A. Stay, F.C. Baker, and D.A. Schooley
(With 4 Figures) ............................. 397
III.6 Environmentally Controlled Changes in Juvenile Hormone
Titers: The Colorado Potato Beetle as a Model System
C.A.D. de Kort and M.A. Khan (With 8 Figures) ........ 407
Contents XI
111.7 The Role of Juvenile Hormone Metabolism in the
Metamorphosis of Selected Lepidoptera
B.D. Hammock, Y.A.1. Abdel-Aal, T. Hanzlik, D. Jones,
G. Jones, R.M. Roe, M. Rudnicka, T.C. Sparks, and
K.D. Wing ................................ 416
III.8 Hemolymph Transport of the Juvenile Hormones
W.G. Goodman, J.T. Trost, C.T. Reiter, D.w. Bean, and
R.O. Carlson (With 3 Figures) ................... 426
111.9 Photoaffinity Labelling of Juvenile Hormone-Binding
Proteins in Leucophaea maderae
J.K. Koeppe, G.E. Kovalick, and G.D. Prestwich
(With 3 Figures) ............................ 438
III.l 0 Regulation of Vitellogenesis in Insects: The Pleiotropic
Role of Juvenile Hormones
F. Engelmann (With 6 Figures) .................. 444
IIl.ll On Titers, Origin, and Functions of Juvenile Hormone III,
Methylfarnesoate, and Ecdysteroids in Embryonic
Development of the Ovoviviparous Cockroach
Nauphoeta cinerea
B. Lanzrein, H. Imboden, C. BUrgin, E. BrUning, and
H. Gfeller (With 5 Figures) ..................... 454
III.12 Defining the Anti-Metamorphic Action of Juvenile
Hormone
J.H. Willis and D.L. Cox (With 4 Figures) ........... 466
1I1.l3 Vitellogenin Synthesis in Locust Fat Body:
Juvenile Hormone-Stimulated Gene Expression
G.R. Wyatt, T.S. Dhadialla, and P.E. Roberts
(With 4 Figures) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 475
Part N Hormonal Mechanisms in Echinoderms
Introduction
J.A. Hoffmann ............................. 486
IV.l Early Steps in Transduction of the Hormonal Signal
Controlling Starfish Oocyte Maturation
P. Guerrier, L. Meijer, M. Moreau, and J.A. Render
(With 3 Figures) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 487
