Table Of ContentBIOSENSORS
– EMERGING MATERIALS
AND APPLICATIONS
Edited by Pier Andrea Serra
Biosensors – Emerging Materials and Applications
Edited by Pier Andrea Serra
Published by InTech
Janeza Trdine 9, 51000 Rijeka, Croatia
Copyright © 2011 InTech
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assumes no responsibility for any damage or injury to persons or property arising out
of the use of any materials, instructions, methods or ideas contained in the book.
Publishing Process Manager Mirna Cvijic
Technical Editor Teodora Smiljanic
Cover Designer Jan Hyrat
Image Copyright Goran Kuzmanovski, 2010. Used under license from Shutterstock.com
First published June, 2011
Printed in Croatia
A free online edition of this book is available at www.intechopen.com
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Biosensors – Emerging Materials and Applications, Edited by Pier Andrea Serra
p. cm.
ISBN 978-953-307-328-6
free online editions of InTech
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Contents
Preface IX
Part 1 Biosensor Technology and Materials 1
Chapter 1 Signal Analysis and Calibration of Biosensors for
Biogenic Amines in the Mixtures of Several Substrates 3
Toonika Rinken, Priit Rinken and Kairi Kivirand
Chapter 2 Molecular Design of Multivalent Glycosides
Bearing GlcNAc, (GlcNAc) and LacNAc – Analysis
2
of Cross-linking Activities with WGA and ECA Lectins 17
Makoto Ogata, Yoshinori Misawa and Taichi Usui
Chapter 3 Determination of Binding Kinetics between
Proteins with Multiple Nonidentical Binding
Sites by SPR Flow Cell Biosensor Technology 35
Kristmundur Sigmundsson, Nicole Beauchemin,
Johan Lengqvist and Björn Öbrink
Chapter 4 Sum-frequency Generation
Spectroscopy in Biosensors Technology 59
Volcke Cédric, Caudano Yves and Peremans André
Chapter 5 How to Make FRET Biosensors
for Rab Family GTPases 81
Nanako Ishido, Hotaka Kobayashi, Yasushi Sako,
Takao Arai, Mitsunori Fukuda and Takeshi Nakamura
Chapter 6 Chiral Biosensors and Immunosensors 99
Marzena Kaniewska and Marek Trojanowicz
Chapter 7 Recent Progress in the Construction Methodology
of Fluorescent Biosensors Based on Biomolecules 123
Eiji Nakata, FongFong Liew, Shun Nakano and Takashi Morii
VI Contents
Chapter 8 “No Calibration” Type Sensor in Routine
Amperometric Bio-sensing - An Example
of a Disposable Hydrogen Peroxide Biosensor 141
C. Creanga, S. Serban, R.W. Pittson and N. El Murr
Chapter 9 QCM Technology in Biosensors 153
Yeison Montagut, José Vicente García, Yolanda Jiménez,
Carmen March, Ángel Montoya and Antonio Arnau
Chapter 10 Electrodeposition of Insulating Thin Film
Polymers from Aliphatic Monomers as
Transducers for Biosensor Applications 179
Guillaume Herlem and Tijani Gharbi
Chapter 11 Surface Modification Approaches
for Electrochemical Biosensors 209
Jin Shi and D. Marshall Porterfield
Chapter 12 Aptamer Sensors Combined with
Enzymes for Highly Sensitive Detection 227
Koichi Abe and Kazunori Ikebukuro
Chapter 13 Enhancing the Performance of Surface-based
Biosensors by AC Electrokinetic Effects - a Review 243
Protiva Rani Roy, Matthew R. Tomkins and Aristides Docoslis
Chapter 14 New Concepts of Integrated Photonic
Biosensors Based on Porous Silicon 265
Cécile Jamois, Cheng Li, Emmanuel Gerelli, Régis Orobtchouk,
Taha Benyattou, Ali Belarouci, Yann Chevolot,
Virginie Monnier and Eliane Souteyrand
Chapter 15 Porous Silicon Sensors
- from Single Layers to Multilayer Structures 291
J.E. Lugo, M. Ocampo, R. Doti and J. Faubert
Chapter 16 Organic-inorganic Interfaces
for a New Generation of Hybrid Biosensors 311
Luca De Stefano, Ilaria Rea, Ivo Rendina, Michele Giocondo,
Said Houmadi, Sara Longobardi and Paola Giardina
Chapter 17 Porous Silicon-based Electrochemical Biosensors 333
Andrea Salis, Susanna Setzu, Maura Monduzzi and Guido Mula
Part 2 Biosensors for Health 353
Chapter 18 Minimally Invasive Sensing 355
Patricia Connolly, David Heath and Christopher McCormick
Contents VII
Chapter 19 Biosensors for Monitoring Autophagy 383
Dalibor Mijaljica, Carlos J Rosado,
Rodney J Devenish and Mark Prescott
Chapter 20 Amperometric Biosensors for Lactate,
Alcohols and Glycerol Assays in Clinical Diagnostics 401
Oleh Smutok, Galina Gayda, Kostyantyn Dmytruk, Halyna Klepach,
Marina Nisnevitch, Andriy Sibirny, Czesław Puchalski, Daniel Broda,
Wolfgang Schuhmann, Mykhailo Gonchar and Vladimir Sibirny
Chapter 21 P450-Based Nano-Bio-Sensors
for Personalized Medicine 447
Camilla Baj-Rossi, Giovanni De Micheli and Sandro Carrara
Chapter 22 Development of Potentiometric Urea Biosensor Based on
Canavalia ensiformis Urease 483
Lívia Maria da Costa Silva, Ana Claudia Sant’Ana Pinto,
Andrea Medeiros Salgado and Maria Alice Zarur Coelho
Chapter 23 Biosensors for Cancer Biomarkers 499
Zihni Onur Uygun and Mustafa Kemal Sezgintürk
Part 3 Biosensors for Environment and Biosecurity 545
Chapter 24 A New Biosensor to Enumerate
Bacteria in Planktonic and Biofilm Lifestyle 547
Maria De Giusti, Francesca Berlutti, Fabrizio Pantanella,
Lucia Marinelli, Alessandra Frioni, Tiziana Natalizi,
Daniela Tufi and Piera Valenti
Chapter 25 Indirect Amperometric Determination
of Selected Heavy Metals Based on
Horseradish Peroxidase Modified Electrodes 569
Philiswa N. Nomngongo, J. Catherine Ngila
and Titus A. M. Msagati
Chapter 26 Chemical Biosensors Based on Proteins
Involved in Biomineralization Processes 589
Rayana R. Ruiz-Arellano, Hugo Javier Serrano-Posada,
María Liliana Marín-García, Bernardo A. Frontana-Uribe
and Abel Moreno
Chapter 27 Applicability of GFP Microbial Whole Cell
Biosensors to Bioreactor Operations - Mathematical
Modeling and Related Experimental Tools 601
Delvigne Frank, Brognaux Alison, Gorret Nathalie,
Sørensen J. Søren, Crine Michel and Thonart Philippe
Preface
A biosensor is defined as a detecting device that combines a transducer with a biologi‐
cally sensitive and selective component. When a specific target molecule interacts with
the biological component, a signal is produced, at transducer level, proportional to the
concentration of the substance. Therefore biosensors can measure compounds present
in the environment, chemical processes, food and human body at low cost if compared
with traditional analytical techniques.
This book covers a wide range of aspects and issues related to biosensor technology,
bringing together researchers from 19 different countries. The book consists of 27
chapters written by 106 authors and divided in three sections. The first section, enti‐
tled Biosensors Technology and Materials, is composed by 17 chapters and describes
emerging aspects of technology applied to biosensors. The subsequent section, entitled
Biosensors for Health and including six chapters, is devoted to biosensor applications
in the medical field. The last section, composed by four chapters, treats of the envi‐
ronmental and biosecurity applications of biosensors.
I want to express my appreciation and gratitude to all authors who contributed to this
book with their research results and to InTech team, in particular to the Publishing
Process Manager Ms. Mirna Cvijic that accomplished its mission with professionalism
and dedication.
Pier Andrea Serra
University of Sassari
Italy
