BioPlexTechnology: an overview of hardware, software

59 Pages·2010·5.96 MB·English

BioPlexTechnology: an overview of hardware, software,
maintenance and data analysis.
Alasdair I. Reid
Field Application Specialist
Bio-Rad Laboratories
Content
•BioPlex Technology: an overview of hardware, software, maintenance
and data analysis.
Alasdair I. Reid; Field Application Specialist, Bio-Rad Laboratories
•Introduction to Bio-Rad
• Understanding Protein Networks
• Why and How of Multiplexing
• xMAP technology
• Bio-Plex hardware and the MCV Plate
• BioPlex software – from Protocols to Data Analysis
• Sample Types and the BioPlex Plate Washer
• Bio-Rad’s products for Cytokine research
• Contacting Bio-Rad Tech Support
Bio-Rad Laboratories
life science research & diagnostics
Driving innovations:
▫ Chromatography Solutions
▫ Electrophoresis Technology
▫ Proteomics and Imaging Systems
▫ Multiplex Protein Assays
▫ Protein Interaction Technology
Functional Protein Analysis –
Understanding Protein Networks
• Cell Physiology
• Cell-to-Cell Interaction
• Influence of External Factors
•Need to understand gene function & protein interaction
•Need to understand cell signaling
•Need to increase efficiency in research and drug discovery
•Need to increase throughput in proteomics research
Protein Analysis single vs multiplex
Protein microarrays and proteomics
Gavin MacBeath, Nature Genetics32,526–532 (2002)
Assay Technologies
from Tubes to Bead Assays
•Technology platform basics
–Industry advances in multiplex and parallel analysis
• Test tubes • Microwell plate • Microarray • Bead Technology:
Microsphere-based
liquid array
Why Multiplex?
•
Decrease precious sample volume requirements
•
Reduce assay reagent volume, expense and labor
•
Include assay controls in each well, rather than on each plate
•
Dramatically increase the amount of information from a single
sample
•
Better correlation of data between different targets (target
analysis is simultaneous for each sample)
xMAP Technology Applications
Life Science Research
• Extracellular signaling proteins
• Intracellular signaling proteins
• Cancer markers
• Cardiac markers
• Endocrine
• Isotyping
Diagnostics
• Metabolic Markers
• Genetic Disease
• Neurobiology
• Allergy Testing
• Transcription Factors
• Autoimmune Disease
• Gene Expression
• HLA Testing
• Genotyping
• Infectious Disease
• MicroRNA profiling
• Biodefense
Bio-Plex: A Flexible
Multiplexing Platform
•
Integrated, easy-to-use 96-well plate-based suspension analysis system.
•
Simultaneous quantitative analysis of up to 100 different proteins, peptides,
DNA fragments and RNA fragments from a single drop of sample.
•
Novel bead-based technology licensed from Luminex Corporation.
•
Often referred to as xMAP technology.
12 years of Luminex Leadership
2010 – BPM 6.0 launched