Bioluminescent Microbial Biosensors: Design, Construction, and Implementation

229 Pages·2015·7.582 MB·English

by  Gerald Thouand, Robert S. Marks

BIOLUMINESCENT
MICROBIAL BIOSENSORS
© 2016 by Taylor & Francis Group, LLC
Pan Stanford Series on the High-Tech of Biotechnology
Robert S. Marks
Series Founding Editor
Avram and Stella Goldstein-Goren Department of Biotechnology Engineering
National Institute for Biotechnology Engineering
Ilse Katz Institute for Nanoscale Science & Technology
Ben Gurion University of the Negev
Israel
Titles in the Series
Published

Vol. 1 Vol. 4
Nanoantenna: Plasmon-Enhanced Nanomaterials for Water
Spectroscopies for Biotechnological Management: Signal Amplification
Applications for Biosensing from Nanostructures
Marc Lamy de la Chapelle and Robert S. Marks and
Annemarie Pucci, eds. Ibrahim Abdulhalim, eds.
2013 2015
978-981-4303-61-3 (Hardcover) 978-981-4463-47-8 (Hardcover)
978-981-4303-62-0 (eBook) 978-981-4463-48-5 (eBook)
Vol. 5
Vol. 2
Bioluminescent Microbial
Viral Diagnostics: Advances and
Biosensors: Design, Construction,
Applications
and Implementation
Robert S. Marks, Leslie Lobel,
Gérald Thouand and Robert S. Marks,
and Amadou Alpha Sall, eds.
eds.
2015
2015
978-981-4364-43-0 (Hardcover) 978-981-4613-65-1 (Hardcover)
978-981-4364-44-7 (eBook)
978-981-4613-66-8 (eBook)
Vol. 3
Forthcoming
Electrochemical Biosensors
Serge Cosnier, ed. Vol. 6
2015 Fibre-Optic Immunosensors and
978-981-4411-46-2 (Hardcover) Biosensors
978-981-4411-47-9 (eBook) Robert S. Marks, ed.
© 2016 by Taylor & Francis Group, LLC
Pan Stanford Series on the High-Tech of Biotechnology
Volume 5
BIOLUMINESCENT
MICROBIAL BIOSENSORS
Design, Construction, and Implementation
edited by
Gérald Thouand
Robert S. Marks

© 2016 by Taylor & Francis Group, LLC
CRC Press
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© 2016 by Taylor & Francis Group, LLC
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Version Date: 20151207
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© 2016 by Taylor & Francis Group, LLC
September30,2015 17:51 PSPBook-9inx6in 00-Gerald-Thouand-prelims
Contents
Preface xi
1 FullyAutomatedFluidicAnalyzerforFoodQuality
AssessmentUsingBioluminescentBiosensors 1
EfstratiosKomaitis,EfstathiosVasiliou,
DimitriosGeorgakopoulos,andConstantinosGeorgiou
1.1 Introduction 1
1.2 AnalyzerDevelopment 3
1.3 Optimization 6
1.4 FunctionalTestoftheFluidicAnalyzer 7
1.5 DeterminationofPhenolicsandApplicationtoOils 8
1.6 DeterminationofAldehydes 11
1.7 Conclusions 12
2 TechnologicalDesignofOpticalBacterialBiosensorsforthe
OnlineEnvironmentalMonitoringofWaterPollutants 15
S.Jouanneau,M.J.Durand,andG.Thouand
2.1 Introduction 15
2.1.1 Liquid-PhaseOpticalMicrobialBiosensors 17
2.1.2 Immobilized-PhaseOpticalMicrobial
Biosensors 18
2.2 Laboratory-DesignedBiosensorsfromtheUMR
GEPEA 19
2.2.1 LumisensI:First-GenerationBiosensor 19
2.2.2 LumisensII:Second-GenerationBiosensor 20
2.2.3 LumisensIII:Third-GenerationBiosensor 21
2.2.4 AdvantagesandDisadvantagesofThree
BacterialBiosensors 23
2.3 LumisensIV:Fourth-GenerationBiosensor 23
© 2016 by Taylor & Francis Group, LLC
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vi Contents
2.4 DescriptionoftheBiosensorLumisensIVforHeavy
MetalDetection 24
2.4.1 BacterialStrains 24
2.4.2 BacteriaPreservation 25
2.5 TechnologicalDescriptionofLumisensIV 27
2.5.1 LiquidCirculation 27
2.5.2 DesignoftheThermoregulationSystem 28
2.5.3 BioluminescenceSignalMeasurement 30
2.5.4 OrganizationofLumisensIV 31
2.6 ComputerInterfaceofBiosensorControlandData
Processing 32
2.6.1 ControlSoftware 32
2.6.2 DataProcessing 33
2.6.2.1 Pictureprocessing 33
2.6.2.2 Dataanalysis 34
2.7 EnvironmentalApplicationoftheBiosensor
LumisensIV 35
2.8 ConclusionandFutureDevelopments 36
3 FiberOpticBiosensorsforEnvironmentalMonitoring 41
EvgeniEltzovandRobertS.Marks
3.1 Introduction 42
3.2 FiberOpticWhole-Cell-BasedBiosensors 45
3.2.1 ConstructionofaWhole-CellFiberOptic
Biosensor 45
3.2.2 BacterialFiberOpticApplications 48
3.2.2.1 Monitoringtoxicchemicalsinsoil 48
3.2.2.2 Determiningtoxicchemicalsinair 49
3.2.2.3 Determiningchemicalsinwater 50
3.3 CreationofaPortableBiosensorforToxicity
Monitoring 52
3.3.1 DeterminingChemicalsinWater 52
3.3.2 DeterminingChemicalsinAir 55
3.3.3 DeterminingChemicalsinSoil 56
3.3.4 ImprovingtheImmobilizationMethodology 57
3.4 ConclusionsandFuturePerspectives 59
© 2016 by Taylor & Francis Group, LLC
September30,2015 17:51 PSPBook-9inx6in 00-Gerald-Thouand-prelims
Contents vii
4 Non-Fiber-OpticBioluminescentBiosensors 67
PhillipMyer,MariadelBusto-Ramos,LisaHartono,
andBruceApplegate
4.1 Introduction 67
4.2 Biosensors 68
4.3 BiosensorLightThroughput 69
4.4 Photomultipliers 71
4.5 EMCCDDevices 75
4.6 Luminometers 76
4.7 Microluminometers 78
4.8 ATPBioluminescence 81
4.9 SiliconPhotomultipliers 83
4.10EnvironmentalBiosensorApplications 84
4.11ConcludingRemarks 86
5 BiosensorwithImmobilizedCellsandLenslessImaging
Detection 91
LucaCevenini,AldoRoda,andElisaMichelini
5.1 Introduction 91
5.2 MainWishedFeaturesoftheDeviceBasedon
ImmobilizedBiosensorsandLenslessImaging
Detection 93
5.3 Descriptions 94
5.3.1 RecombinantStrains 94
5.3.2 ImmobilizationoftheBioreporters 96
5.3.3 Device 98
5.3.4 ElectronicandDataProcess 100
5.4 ProofofConcept 101
6 ABiosensorwithGeneticallyModifiedBacteriaImmobilized
onaFiberandaGlassSlide 107
MarjolijnWoutersen,BramvanderGaag,JanMink,
RobertS.Marks,BramBrouwer,andMinneB.Heringa
6.1 Introduction 107
6.2 DescriptionoftheStrainandGrowthConditions 109
6.3 ImmobilizationoftheStraininaSol-Gel 110
6.4 ConstructionoftheDevice 111
© 2016 by Taylor & Francis Group, LLC
September30,2015 17:51 PSPBook-9inx6in 00-Gerald-Thouand-prelims
viii Contents
6.5 ElectronicsandDataProcessing 114
6.6 InfluenceofNutrientAvailabilityontheResponse 114
6.7 TheEffectofBiofouling 115
6.8 DeterminationofSensitivity 117
6.9 DelayedExposure 120
6.10ComparisonofImmobilizationMethods 120
6.11EvaluationoftheUVDisinfectionUnit 122
6.12Conclusions 123
7 DevelopmentandCharacterizationofaLiving-Cell
BioluminescentBioreporterIntegratedCircuit 127
JamesT.Fleming,SyedIslam,NoraDianneBull,
MichaelSimpson,andGarySayler
7.1 Introduction 127
7.2 DevelopmentoftheIntegratedCircuitComponent 128
7.2.1 TheFirst-GenerationBBIC 130
7.2.2 TheSecond-GenerationBBIC 132
7.2.3 TheThird-GenerationBBIC 134
7.3 CharacterizationoftheThird-GenerationBBIC 135
7.4 MicroluminometerPerformance 136
7.5 BBICInstrumentation 139
7.5.1 Six-ChipTestBed 139
7.5.2 BBICWand 141
7.5.3 BBICCD 142
7.6 BBICPrototypeTesting 144
7.7 Conclusions 147
8 BODSensorwithImmobilizedLuminousCellsonChip 151
ToshifumiSakaguchiandEiichiTamiya
8.1 Introduction 151
8.2 IsolationandPreparationofLuminous
Microorganisms 152
8.3 ConstructionofanOnsiteDetectionSystem 154
8.4 BiochipPreparationandCalibration 155
8.5 PracticalApplicationandToxicantDetection 157
8.6 Conclusions 159
© 2016 by Taylor & Francis Group, LLC
September30,2015 17:51 PSPBook-9inx6in 00-Gerald-Thouand-prelims
Contents ix
9 BioluminescentWhole-CellSensors:IntegrationofBacterial
ReporterCellsontoHardwarePlatforms 163
SaharMelamedandShimshonBelkin
9.1 Introduction 163
9.2 LiveCellPatterningandDepositionTechniques 164
9.2.1 InanimateSurfacesforLiveCellDeposition 164
9.2.2 SoftLithography 165
9.2.3 CellPrinting 166
9.3 CellImmobilization 168
9.3.1 NakedAttachment:AnimateandInanimate
SurfaceModifications 168
9.3.2 ImmobilizationbyDepositionofEncapsulated
Cells 172
9.4 Long-TermMaintenanceofViabilityandActivity 174
9.5 Summary 176
10 LuminescentBiosensingSystemsBasedonGenetically
EngineeredSpore-FormingBacteria 187
LeslieD.Knecht,PatriziaPasini,andSylviaDaunert
10.1 Introduction 187
10.2 Whole-CellBiosensors 189
10.2.1 ArsenicSpore-BasedWhole-CellBiosensor 191
10.2.2 ZincSpore-BasedWhole-CellBiosensor 195
10.3 ApplicationofSpore-BasedWhole-CellBiosensors
toPhysiologicalandEnvironmentalSamples 198
10.4 ExtremeConditions 201
10.5 PackagingofSporesforFieldApplications 204
10.5.1 MicrofluidicPlatforms 205
10.5.2 Paper-BasedSensors 210
10.6 Conclusions 213

Index 219
© 2016 by Taylor & Francis Group, LLC