Table Of ContentI
Recent Results in Cancer Research
SPecial Supplement
Biology of A.mphibian
Tumors
Edited by
Merle Mizell
with 186 Figures
Springer-Verlag
New York-Heidelberg-Berlin
1969
Symposium: Biology of Amphibian Tumors
Sponsored by:
Tulanl' University; Cancer Association of
Greater New Orleans, Inc.; National Canccr Institute
October 28, 29, 30, 1968
ISBN-13: 978-3-642-85793-5 e-ISBN-13: 978-3-642-85791-1
DOl: 10.1007/978-3-642-85791-1
All rights reserved.
No part of this book may be translated or reproduced in any form
without written permission from Springer-Verlag.
© 1969 by Springer-Verlag New York Inc.
Softeover reprint of the hardcover 1st edition 1969
Library of Congress Card Number 72-101624
Title No. 1646
To the memory of
DR. GLADYS M. MATEYKO
(August 22, 1922-0ctober 11, 1968)
Dr. Mateyko, the first woman to receive a Fellowship from the Damon Runyon
Memorial Fund for Cancer Research, was an active investigator in the field of cancer
research, and especially amphibian tumor studies. Her friends and colleagues dedicate
this volume to her memory.
Contents
Preface xi
Introduction Xll1
List of Participants. xv
Introduction
State of the Art: Lucke Renal Adenocarcinoma. MERLE MIZELL 1
Biology of Amphibians
Interrelations of the Populations of the Rana pipiens Complex. JOHN A. MOORE 26
Spontaneous and Experimental Mutations in the Newt Pleurodeles Waltlii Mi-
chah. LOUIS GALLIEN 35
Nucleo-Cortical Interactions During Amphibian Development. PHILIP GRANT 43
Amphibian Cells in vitro
Mass Culture of Amphibian Cells: Methods and Observations Concerning Sta-
bility of Cell Type. KEEN A. RAFFERTY, JR. 52
Dependence of Tumor Formation in Frogs on Abnormal Nucleolar Function.
WILLIAM R. DURYEE 82
Characteristics of Cell Lines from Haploid and Diploid Anuran Embryos.
JEROME J. FREED, LISELOTTE MEZGER-FREED and SUSAN A. SCHATZ 101
Species Identification of Poikilothermic Tissue Culture Cells.
ARTHUR E. GREENE, LEWIS L. CORIELL and JESSE CHARNEY 112
Amino Acid and Nucleoside Incorporation in Frog Kidney Cells: An Autoradio-
graphic Study. DANIEL MALAMUD and JOHN TINKER 121
Immunity and Tolerance in Amphibia
Immunity and Tolerance in Amphibia. EDWIN COOPER 130
Immunologic Tolerance and Blood Cell Chimerism in Experimentally Produced
Parabiotic Frogs. E. PETER VOLPE, BRYAN M. GEBHARDT, SHERILL CURTIS,
and ELIZABETH M. EARLEY 13 7
vii
viii CONTENTS
Immunogenetic and Developmental Aspects of Tissue Transplantation Immunity
in Urodele Amphibians. NICHOLAS COHEN 153
Immunoglobulin and Complement Systems of Amphibian Serum.
DONALD W. LEGLER, E. EDWARD EVANS, PETER F. WEINHEIMER,
RONALD T. ACTON and MARIE H. ATTLEBERGER 169
The Amphibian as a Key Step in the Evolution of Lymphoid Tissue and Diverse
Immunoglobulin Classes.
B. POLLARA, W. A. CAIN, J. FINSTAD and R. A. GOOD 177
Tumors and Related Pathology in Amphibia
Some Morphological and Biological Characteristics of a Tumor of the Newt,
Triturus cristatus Laur. VINCENZO G. LEONE and TERESA ZAVANELLA 184
Skin Carcinogenesis, Mammals Versus Amphibia. BEPPINO C. GIOVANELLA 195
Plasmacytoma in a Rana pipiens.
SYDNEY S. SCHOCHET, JR. and PETER W. LAMPERT 204
Structures of Spontaneous and Transplanted Tumors in the Axolotl (Siredol1
mexicanum). VICTOR V. BRUNST 215
Tumors of the Testis in the Mexican Axolotl (Ambystoma or Siredon mexi-
canum). R. R. HUMPHREY 220
Distribution and Transmission of the Lucke Renal Adenocarcinoma
Simulated Transmission of Renal Tumors III Oocytes and Embryos of Rana
pipiens. KENYON S. TWEEDELL 229
Non-Specific Transmission of the Lucke Tumor. DONALD J. MULCARE 240
Lucke Renal Adenocarcinoma: Epidemiological Aspects.
ROBERT GILMORE McKINNELL 254
Chromosome Studies of Primary Renal Carcinoma from Vermont Rana pijJiens.
MARIE A. DIBERARDINO and NANCY HOFFNER 261
Viruses of Amphibia
Studies on the Viral Etiology of the Renal Adenocarcinoma of Rana pipiens
(Lucke Tumor).
ALLAN GRANOFF, MANETH GRAVELL and R. W. DARLINGTON 279
CONTENTS ix
Fine Structure Studies of Cytoplasmic Viruses Associated with Frog Tumors.
PHILIP D. LUNGER 296
Comparative Studies of Amphibian Cytoplasmic Virus Strains Isolated from the
Leopard Frog, Bullfrog, and Newt. H. FRED CLARK, CLAUDIA GRAY,
FRANCES FABIAN, ROBERT F. ZEIGEL and DAVID T. KARZON 310
Tadpole Edema Virus: Pathogenesis and Growth Studies and Additional Sites
of Virus Infected Bullfrog Tadpoles.
KEN WOLF, G. L. BULLOCK, C. E. DUNBAR and M. C. QUIMBY 327
The Herpestype Virus of the Lucke Renal Adenocarcinoma
H~rp~stype Virus Latency in the Lucke Tumor.
MERLE MIZELL, CHRISTOPHER W. STACKPOLE and J. JOYCE ISAACS 337
Density Gradient Centrifugation Studies on the Herpes-type Virus of the Lucke
Tumor. IRV TOPLIN, PHILLIP M. BRANDT and PETER SOTTONG 348
Reactivity of Serum from Frogs and Other Species with a Herpesvirus Antigen
Extracted from a Burkitt Lymphoma Cultured Cell Line.
MARY A. FINK, GLADYS KING and MERLE MIZELL 358
A Preliminary Report on the Serology of Lucke and Burkitt Herpes-type Viruses:
A Shared Antigen. JOHN M. KIRKWOOD, GAYLA GEERING, LLOYD J. OLD,
MERLE MIZELL and JOHN WALLACE 365
Lymphoid Diseases in Amphibia
Possible Immunological Factors III Amphibian Lymphosarcoma Development.
LAURENS N. RUBEN 368
Organ Cultures of Normal and Neoplastic Amphibian Tissues.
MICHAEL BALLS, JOHN D. SIMNETT and ELIZABETH ARTHUR 385
Acceptance and Regression of a Strain-specific Lymphosarcoma III Mexican
Axolotls. LOUIS E. DELANNEY and KATE BLACKLER 399
Biologically Defined Strains of Amphibia
Development of Biologically Defined Strains of Amphibians.
GEORGE W. NACE and CHRISTINA M. RICHARDS 409
Diseases in an Amphibian Colony. GERALD D. ABRAMS 419
x CONTENTS
Neoplasia in Higher Vertebrates: Relevance to Amphibian Tumors
A. INVESTIGATIONAL APPROACHES
Some Comparative Morphological Aspects of Renal Neoplasms in Ral1a pipiens
and of Lymphosarcomas in Amphibia. CLYDE J. DAWE, JR. 429
Immunological Approaches to the Study of Viral Antigens Associated with
Neoplasms. PAUL GERBER 441
Nucleic Acid Homology as Applied to Investigations on the Relationship of
Viruses to Neoplastic Diseases. MAURICE GREEN 445
B. TUMORS AND ASSOCIATED HERPES VIRUSES
Viruses Associated with Burkitt's Lymphoma. ROBERT A. MANAKER 455
A Herpes Virus as a Cause of Marek's Disease in Chickens.
B. R. BURMESTER, R. L. WITTER, K. NAZERIAN and H. G. PURCHASE 460
Studies on the Viral Etiology of Marek's Disease of Fowl. DALE J. RICHEY 469
Herpes Simplex Viruses and Human Cancer: Current Status of the Problem.
BERNARD ROIZMAN 478
Summation and Perspectives
Summation and Perspectives. KARL HABEL 482
Preface
New Orleans has a distinguished history as a center for medical and biological
learning, a history shared by Tulane University, its School of Medicine, and its Bio
logical and Medical Sciences departments. This background made it especially fitting
that the University, in conjunction with the Cancer Association of Greater New
Orleans, Inc. and the National Cancer Institute, should sponsor the "Symposium:
Biology of Amphibian Tumors" held October 28, 29, 30, 1968. The University wishes
to express its appreciation to the Cancer Association for its assistance in making the
Symposium possible and to acknowledge the support made available through the Bio
medical Sciences Support Grant program of the National Institutes of Health.
As the title of this volume indicates, the Symposium yielded valuable results in
the area of cancer research and it stands to stimulate further efforts in this most
important field. Some notion of the impact of this symposium is suggested by the
broad range of the 200 participants it attracted. They came not only from the breadth
and length of the U.S., but from abroad, from France, England, Austria, and Italy.
No international convocation can reach any measure of success without the aid
of a great many individuals. For the grand concept of the Symposium, credit must
go to the members of the ad hoc organizational committee; and for the many local
arrangements which provided the proper atmosphere for the exchange of information,
we are indebted to all of the members of the New Orleans Host Committee.
It was Tulane's privilege to playa part in the presentation of the Symposium.
DAVID R. DEENER, Ph.D.
Provost and Dean of the Graduate School
Tulane University
The Cancer Association of Greater New Orleans and its parent organization, The
United Fund of Greater New Orleans, are proud to have had the opportunity to co
sponsor the symposium in which these papers were presented. It was an exciting
experience for our group to be involved in a meeting which brought together tumor
researchers from such a variety of disciplines and geographical locations. The exchange
of ideas fostered by such a gathering is a valuable goal in itself; however, it is par
ticularly satisfying to realize that the effects of the symposium will extend far beyond
the meeting itself because of this monograph, which will make the ideas available to
cancer researchers throughout the world. We are most happy that we could extend
the goal of our organization by contributing to such an endeavor.
SAMUEL LOGAN, M.D.
President, Cancer Association of
Greater New Orleans, Inc.
Description:New Orleans has a distinguished history as a center for medical and biological learning, a history shared by Tulane University, its School of Medicine, and its Bio logical and Medical Sciences departments. This background made it especially fitting that the University, in conjunction with the Canc
