Biology and Chemistry of Eukaryotic Cell Surfaces

368 Pages·1974·6.17 MB·English

by  E.Y.C. Lee (Eds.)

minmi uimTER svmposm
1. W. J. Wheian and J. Schultz, editors. HOMOLOGIES IN ENZYMES AND META-
BOLIC PATHWAYS and METABOLIC ALTERATIONS IN CANCER,* 1970
2. D. W. Ribbons, J. F. Woessner, Jr., and J. Schultz, editors-. NUCLEIC ACID-PROTEIN
INTERACTIONS and NUCLEIC ACID SYNTHESIS IN VIRAL INFECTION,* 1971
3. J.F. Woessner, Jr. and F. Huijing, editors: THE MOLECULAR BASIS OF BIOLOGICAL
TRANSPORT, 1972
4. J. Schultz and B. F. Cameron, editors·. THE MOLECULAR BASIS OF ELECTRON
TRANSPORT, 1 972
5. F. Huijing and E. Y. C. Lee, editors·. PROTEIN PHOSPHORYLATION IN CON-
TROL MECHANISMS, 1973
6. J. Schultz and H. G. Gratzner, editors·. THE ROLE OF CYCLIC NUCLEOTIDES
IN CARCINOGENESIS, 1973
7. E. Y. C. Lee and E. E. Smith, editors·. BIOLOGY AND CHEMISTRY OF EUCARYOTIC
CELL SURFACES, 1974
8. J. Schultz and R. Block, editors·. MEMBRANE TRANSFORMATION IN NEOPLASIA,
1974
Published by North-Holland Publishing Company, Amsterdam, The Netherlands.
minmi uiinTER svmposin-voiumE 7
Biology and Chemistry
of Eucaryotic Cell
Surfaces
edited by
E. V. I. Lee
E. E. Smith
DEPARTMENT OF BIOCHEMISTRY
UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
MIAMI, FLORIDA
Proceedings of the Miami Winter Symposia, January 14- 15, 1974
organized by the Department of Biochemistry, University of Miami
School of Medicine, Miami, Florida
ACADEMIC PRESS New York and London 1974
A Subsidiary of Harcourt Brace Jovanovich, Publishers
COPYRIGHT © 1974, BY ACADEMIC PRESS, INC.
ALL RIGHTS RESERVED.
NO PART OF THIS PUBLICATION MAY BE REPRODUCED OR
TRANSMITTED IN ANY FORM OR BY ANY MEANS, ELECTRONIC
OR MECHANICAL, INCLUDING PHOTOCOPY, RECORDING, OR ANY
INFORMATION STORAGE AND RETRIEVAL SYSTEM, WITHOUT
PERMISSION IN WRITING FROM THE PUBLISHER.
ACADEMIC PRESS, INC.
Ill Fifth Avenue, New York, New York 10003
United Kingdom Edition published by
ACADEMIC PRESS, INC. (LONDON) LTD.
24/28 Oval Road, London NW1
Library of Congress Cataloging in Publication Data
Main entry under title:
Biology and chemistry of eucaryotic cell surfaces.
(Miami winter symposia, v. 7)
"Proceedings of the Miami winter symposia, January
14-15, 1974, organized by the Department of Biochemistry,
University of Miami School of Medicine, Miami, Florida."
1. Cell membranes—Congresses. 2. Cytochemistry-
Congresses. I. Lee, Ernest Yee Chung, ed. II. Smith,
Eric Ernest, Date ed. III. Miami, University of,
Coral Cables, Fla. Dept. of Biochemistry. IV. Series.
[DNLM: 1. Cell membrane-Congresses. 2. Cell
nucleus-Congresses. W3M1202v. 7 1974 / WH595 S989b
1974]
QH601.B525 574.8'75 74-10683
ISBN 0-12-441550-4
PRINTED IN THE UNITED STATES OF AMERICA
SPEAKERS, CHAIRMEN,
AND DISCUSSANTS
Ankel, H., The Medical College of Wisconsin, Milwaukee, Wisconsin
Assaf, S. A., University of Texas Medical School, Houston, Texas
Basu, S., University of Notre Dame, Notre Dame, Indiana
Behrens, N. H., Instituto de Investigaciones Bioquimicas, Buenos Aires,
Argentina
Bernacki, R., Roswell Park Memorial Institute, Buffalo, New York
Bhattacharyya, S., Duke University Medical Center, Durham, North Carolina
Boone, C, National Institutes of Health, Bethesda, Maryland
Bretthauer, R. K., University of Notre Dame, Notre Dame, Indiana
Christopher, C. W., Harvard Medical School, Boston, Massachusetts
Czech, M. P., Duke University Medical Center, Durham, North Carolina
Daniel, J., Papanicolaou Cancer Institute, University of Miami, Miami,
Florida
DeLuca, L., National Institutes of Health, Bethesda, Maryland
Elbein, A. D., University of Texas Health Science Center, San Antonio,
Texas
Fullerton, W. W., Merck Institute for Therapeutic Research, West Point,
Pennsylvania
Gardner, D. A., Miles Laboratories, Inc., Elkhart, Indiana
Gerisch, G., Friedrich-Miescher-Laboratorium des Max Planck Gesellschaft,
Tübingen, Germany
Ginsburg, V., National Institute of Arthritis and Metabolic Disorders,
Bethesda, Maryland
ix
SPEAKERS, CHAIRMEN, AND DISCUSSANTS
Glick, M. C, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania
Goldstein, J., New York Blood Center, New York, New York
Hakomori, S., University of Washington, Seattle, Washington
Heath, E. C, University of Pittsburgh School of Medicine, Pittsburgh,
Pennsylvania
Hempling, H. G., Medical University of S. Carolina, Charleston, South
Carolina
Hochstadt, J., Worcester Foundation for Experimental Biology, Shrewsbury,
Massachusetts
Horowitz, M., New York Medical College, Valhalla, New York
Huijing, F., (Session Chairman), University of Miami School of Medicine,
Miami, Florida
Jamieson, G. A., The American National Red Cross Blood Research
Laboratory, Bethesda, Maryland
Jeanloz, R. W., Harvard Medical School, Boston, Massachusetts
Kalos, J., Columbia University, New York, New York
Keston, A., Institute for Medical Research and Studies, New York,
New York
Koch, G., Roche Institute of Molecular Biology, Nutley, New Jersey
Kornfeld, S. A., Washington University, St. Louis, Missouri
Leloir, L. F., (Session Chairman), Instituto de Investigaciones Bioquimicas,
Buenos Aires, Argentina
Longton, R., National Naval Medical Center, Bethesda, Maryland
Lubin, M., Dartmouth Medical School, Hanover, New Hampshire
Lynen, F., (Session Chairman), Max-Planck Institut für Biochemie, Munich,
Germany
Marchesi, V. T., Yale University, New Haven, Connecticut
Montes de Gomez, V., National University of Colombia, Bogota, Colombia
x
SPEAKERS, CHAIRMEN, AND DISCUSSANTS
Morrison, M., St. Jude Children's Research Hospital, Memphis, Tennessee
Nathenson, S. G., Albert Einstein College of Medicine, New York, New York
Nicolson, G. L., The Salk Institute for Biological Studies, San Diego,
California
Ostrand-Rosenberg, S., California Institute of Technology, Pasadena,
California
Pickart, L. R., University of California, San Francisco, California
Poretz, R. D., Rutgers University, New Brunswick, New Jersey
Quash, G., University of the West Indies, Kingston, Jamaica
Rabinowitz, M., National Institutes of Health, Bethesda, Maryland
Reddi, A. H., University of Chicago, Chicago, Illinois
Reinhold, V. N., Arthur D. Little, Inc., Cambridge, Massachusetts
Rieber, M., Center of Microbiology and Cell Biology, Caracas, Venezuela
Robbins, P. W., Massachusetts Institute of Technology, Cambridge,
Massachusetts
Roseman, S., John Hopkins University, Baltimore, Maryland
Rosenberg, S., (see S. Ostrand-Rosenberg)
Roth, J., Biocenter, Basel, Switzerland
Schenkein, L, New York University Medical Center, New York, New York
Srere, P. A., Veterans Administration Hospital, Dallas, Texas
Stanford, H. K., (Session Chairman), President, University of Miami,
Coral Gables, Florida
Sutherland, E. W., (Session Chairman), University of Miami School of
Medicine, Miami, Florida
Vaheri, A., University of Helsinki, Helsinki, Finland
Warren, L., University of Pennsylvania, Philadelphia, Pennsylvania
xi
SPEAKERS, CHAIRMEN, AND DISCUSSANTS
Whelan, W. /., Chairman, Department of Biochemistry, University of Miami
School of Medicine, Miami, Florida
Wolff /., National Institutes of Health, Bethesda, Maryland
Wood, H. G., (Session Chairman), Case Western Reserve University,
Cleveland, Ohio
PREFACE
In January 1969, the Department of Biochemistry of the University of
Miami and the University-affiliated Papanicolaou Cancer Research Institute
joined in sponsoring and presenting two symposia on biochemical topics.
Those symposia have now developed into an annual event, now in its sixth
year. In 1970 the two symposia were published as the first volume of a
continuing series entitled the "Miami Winter Symposia." In 1972 we initiated
the publication of the proceedings of the two symposia as separate volumes
in the series.
The major emphasis in the selection of the topics for our symposia has
been to identify the frontier areas in which progress in biochemistry is
leading toward an understanding of the molecular bases of biological pheno-
mena. We follow a pattern in which a common theme is dealt with, in our
symposium, on a fundamental basis, and then in the Papanicolaou Cancer
Research Institute Symposium, as it relates to an understanding of malignant
processes. This volume, the seventh in the series, contains the proceedings of
the Biochemistry Department's Symposium on the "Biology and Chemistry
of Eucaryotic Cell Surfaces," and will be published simultaneously with the
proceedings of the Papanicolaou Cancer Research Institute's symposium on
"Membrane Transformations in Neoplasia."
In 1975 the topics for the symposium will be on a theme in basic
immunology followed by a symposium on cancer immunology, taking place
during 13-17 January.
Associated with the symposia is a featured lecture, the Feodor Lynen
Lecture, named in honor of the Department of Biochemistry's distinguished
Visiting Professor. Past speakers were Drs. George Wald, Arthur Kornberg,
Harland G. Wood, Earl W. Sutherland, Jr., and this year we heard from an
old friend of the Department, Dr. Luis F. Leloir. These lectures are of an
autobiographical nature, devoted to the life histories of these great scientists.
They are greatly enjoyed by their audience, and for ourselves, remain a
source of inspiration. The Lynen lecturer for 1975 will be Dr. Gerald M.
Edelman.
Our arrangement with the speakers and publishers is to ensure publica-
tion as rapidly as possible. We thank the secretarial staff for their help in
bringing this about. To bring forward as much of the recent work as possible,
xiii
PREFACE
we now include a session of short communications in the meeting, the
abstracts of which are included in this volume. We thank the speakers for
submitting their manuscripts promptly and the participants whose interest
and discussions provided the interactions that bring a symposium to life.
The financial assistance of the University of Miami Departments of
Anesthesiology and Medicine, the Howard Hughes Medical Institute, Derma-
tology Foundation of Miami, Eli Lilly and Company, Hoffmann-La Roche
Inc., MC/B Manufacturing Chemists and the International Union of Bio-
chemistry, is gratefully acknowledged. The Union provided funds for the
participation of Latin-American biochemists. This symposium volume also
constitutes volume 62 of the IUB series.
E. Y. C. Lee
E. E. Smith
XIV
THE FIFTH FEODOR LYNEN LECTURE
"I HATE TO BORE PEOPLE WITH MY RECOLLECTIONS"
L.F. LELOIR
Instituto de Investigaciones Bioquimicas
"Fundacion Campomar", y Facultad de Ciencias
Exactas y Naturales
When Bill Whelan invited me to give the Lynen
Lecture, which is of an autobiographical nature, I accept-
ed with reluctance because I don't think that anything in
my life is worth telling and much less, entertaining.
Furthermore, I have already written an historical review
called "Polysaccharide synthesis seen from Buenos Aires"
(1) and it is difficult to write two biographies without
having led a double life.
My first difficulty with this lecture was that I
could not find a title. I spent such a long time think-
ing, that Whelan phoned me and it was then that I said
"I hate boring people with my recollections". The long
distance call was rather noisy and Whelan took that phrase
as the title. Since I did not find a better one it was
left as it is.
My life covers the period of biochemistry in
which most of the vitamins, hormones, enzymes and coenzymes
were discovered and in which most of the reactions of
intermediary metabolism were unravelled. I was born in
1906, the same year in which Harden and Young (2) pub-
lished their paper on the coenzyme of yeast fermentation
and which initiated the studies on cofactors in which we
became involved many years later.
After fairly normal studies in the primary and
secondary school, I studied medicine, in Buenos Aires. I
was an average student, interested in scientific subjects
but had no occasion of thinking on research. This hap-
pened while I was working in the hospital and I heard
that Dr. Houssay was doing very interesting studies on
carbohydrate metabolism. They were described to me as
1