Bile Acid Biology and Therapeutic Actions Abstracts Poster Abstracts

193 Pages·2008·0.81 MB·English

Falk Symposium 165
XX International Bile Acid Meeting
Bile Acid Biology and
Therapeutic Actions
June 13–14, 2008
Hotel Okura
Amsterdam,
The Netherlands
Abstracts
Poster Abstracts
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AbstractsofInvitedLectures
PosterAbstracts
FalkSymposium165
XXInternationalBileAcidMeeting
BILEACIDBIOLOGYAND
THERAPEUTICACTIONS
Amsterdam(TheNetherlands)
June13–14,2008
President:
P.L.M.Jansen,Amsterdam(TheNetherlands)
ScientificOrganization:
D.Keppler,Heidelberg(Germany)
U.Beuers,Amsterdam(TheNetherlands)
A.Stiehl,Heidelberg(Germany)
M.Trauner,Graz(Austria)
CONTENTS
page
SessionI
Metabolismandtransportofbileacids
Chair:
C.A.Einarsson,Huddinge
J.Sjövall,Stockholm
Membraneasymmetryandtheregulationofbileformation
R.P.J.OudeElferink,Amsterdam 23–24
ABCB11(BSEP)activityisdependentonthecanalicular
membranecholesterolcontent
C.C.Paulusma,Amsterdam 25
Fic1deficientrathepatocytesrevealstructuralandfunctional
defectsinthebilecanalicularmembranewhenexposed
tohydrophobicbileacids
J.L.Boyer,NewHaven 26
Bileacidsandthebrain.Suggestedpathogeneticmechanism
inconnectionwithformationofbrainxanthomasinpatients
withCTX
I.Björkhem,Huddinge 27
SessionII
Transportofbileacids
Chair:
P.A.Dawson,Winston-Salem
D.Keppler,Heidelberg
Canalicularmicrodomainsandbileformation
B.Stieger,Zurich 31
Regulationofthecellsurfaceexpressionandtransport
capacityofBSEPbysmallchemicalmolecules
H.Hayashi,Tokyo 32
3
TheroleofABCB11(BSEP)variationinsusceptibilityto
intrahepaticcholestasisofpregnancy
P.H.Dixon,London 33
Intracellulartransportofbilesalts
K.N.Faber,Groningen 34
Conjugatedbileacidsregulatehepaticgluconeogenicgenes
viaGαiproteincoupledreceptor(s)intheliver
P.B.Hylemon,Richmond 35
SessionIII
Transportandactionsofbileacids
Chair:
W.Kramer,Frankfurt
F.J.Suchy,NewYork
RoleoforganicsolutetransporterOSTα-OSTβinintestinal
basolateralbileacidtransportandhomeostasis
P.A.Dawson,Winston-Salem 39
Mechanismofregulationofbileacidtransportinthesmall
intestine
H.Suzuki,Tokyo 40
Expressionandlocalizationofthemembrane-boundbileacid
receptorTGR5inhumangallbladdertissue
V.Keitel,Düsseldorf 41
Bilesaltscontroltheantibacterialpeptidecathelicidinthrough
nuclearreceptorsinhumanbiliaryepithelialcells
N.Chignard,Paris 42
Chair:
P.L.M.Jansen,Amsterdam
G.A.Kullak-Ublick,Zurich
FromGlarustoGallenfarbstoffen:Thescientificcontributions
ofRudiSchmid(Noabstract)
A.F.McDonagh,SanFrancisco
4
SessionIV
Nuclearreceptorregulation
Nuclearreceptorregulationofbileacidtransporters
G.A.Kullak-Ublick,Zurich 45–46
OrphannuclearreceptorLXRinbileaciddetoxificationand
cholestasis
W.Xie,Pittsburgh 47
ThenuclearhormonereceptorERalphaplaysacriticalrole
indeterminingestrogen-inducedcholesterolgallstones
D.Q.-H.Wang,Boston 48–49
RoleofintestinalnuclearbileacidreceptorFXRinthegut-liver
axis
A.Moschetta,Bari 50
SessionV
Bileacidsasmetabolicintegrators
Chair:
A.F.Hofmann,SanDiego
M.Trauner,Graz
LRH-1andcontrolofbileacidhomeostasis
K.Schoonjans,Illkirch 53
Differentialeffectsofursodeoxycholicacid(UDCA)and
side-chain-shortenednorUDCAinthetreatmentoffatty
liverandatherosclerosis
M.Trauner,Graz 54
Side-chain-modificationcriticallydeterminesthephysiologicand
therapeuticpropertiesof24-nor-ursodeoxycholicacidinthe
treatmentofsclerosingcholangitisinMdr2(Abcb4)knock-out
miceandisolatedbileductunits
E.Halilbasic,Graz 55
Roleofsidechainamidationfortheanticholestaticactionof
norUDCAinratliver
U.Beuers,Amsterdam 56
PresentationoftheAdolfWindausPrize
G.Paumgartner,Munich
5
AdolfWindausPrizeLecture
OSTα-OSTβasapotentialtherapeutictargetforaltering
bileacidandlipidlevels
N.Ballatori,Rochester,NY 57–58
SessionVI
Bileacids:Cellularinjuryandregeneration
Chair:
D.Häussinger,Düsseldorf
R.Poupon,Paris
Acrobaticliverstemcells:Roleincellularinjuryand
regeneration
T.Roskams,Leuven 61–62
Pro-andantiapoptoticactionsofbileacidsandCD95ligand
inhepaticstellatecells
D.Häussinger,Düsseldorf 63–64
SuccessfulreactivationofhepaticMrptransportersinendotoxin
inducedcholestasisinmicebytheCARactivator6,7-dimethyl-
esculetin
I.V.Martin,Aachen 65
Neuropeptides,neuroendocrinehormonesandbileacid
interactionsincholangiocyteresponsetoinjury
A.Benedetti,Ancona 66
SessionVII
Bileacid-relatedgeneticliverdisease
Chair:
G.P.vanBergeHenegouwen,Utrecht
H.-U.Marschall,Huddinge
ThemanyfacesofMDR3deficiency
P.L.M.Jansen,Amsterdam 69–71
Severebilesaltexportpumpdeficiency:Mutations,
immunohistochemistryandmalignancyrisk
S.Strautnieks,London 72–73
6
Roleofgeneticsindrug-inducedcholestasis
C.Pauli-Magnus,Basel 74
Variabilityofcholestaticliverdiseaseinafamilywith
11siblingsandanABCB4defect
D.Gotthardt,Heidelberg 75
ABCG5/G8asahumanriskgeneforcholesterolgallstone
disease
F.Lammert,Homburg/Saar 76–77
ATP8B1functionandpossibletherapeuticinterventions
R.H.J.Houwen,Utrecht 78
Bileacidabnormalitiesinperoxisomaldisorders
S.Ferdinandusse,Amsterdam 79
SessionVIII
Bileacidsastherapeuticagents
Chair:
R.W.Chapman,Oxford
G.Paumgartner,Munich
UrsodeoxycholicacidforPSC–Anupdate
K.D.Lindor,Rochester,MN 83
Excellentlong-termsurvivalinpatientswithprimarybiliary
cirrhosistreatedwithursodeoxycholicacid
A.Parés,Barcelona 84–85
High-doseUDCAforthetreatmentofNASH:Resultsofa
multicentertrial(Noabstract)
U.Leuschner,Frankfurt
IntravenoususeofSUDCA(ursodeoxycholicdisulfate)–
Aunidirectionalbileacid,forhepatoprotectioninliver
transplantationandTPN-associatedcholestasis
K.D.R.Setchell,Cincinnati 86–88
Features,prognosisandmanagementofPBCintheera
ofUDCAandbudesonide
R.Poupon,Paris 89–90
ListofSpeakers,ModeratorsandScientificOrganizers 91–95
7
PosterAbstracts
IMetabolismandtransportofbileacids
1. Sulphatedprogesteronemetabolitesinterferewithprimaryhumanhepatocyte
bileacidtransport
S.Abu-Hayyeh,C.Selden,M.R.Romero,H.-U.Marschall,M.Rees,R.White,
C.Williamson(London,GB;Huddinge,S;Basingstoke,GB)
2. BiliaryandfaecalbileacidprofilesinducedbyLarreatridentatainhamster
S.Arteaga,R.Cárdenas(Mexico,MEX)
3. ThehydrophobiciminosugarAMP-DNMincreasesbiliarylipidsecretionin
C57BL/6miceviaFGFmediatedregulationofCYP7A1
N.Bijl,R.Ottenhoff,C.P.A.A.Roomen,S.Scheij,J.M.F.G.Aerts,A.K.Groen
(Amsterdam,NL)
4. Formationofbilecanaliculiandextracellularmatrix(ECM)-cellcontacts
D.Cassio,S.Kassar,D.Jaillard,J.Degrouard,B.Grosse(Orsay,F)
5. Fastmethodstomeasureamidatedbileacidsinlivertransplantpatients:
MS/MScharacterizationofside-chainanalogfluorescentderivatives
H.E.Ferreira,J.Barbosa,H.Vilca-Melendez,N.D.Heaton,J.C.M.Bordado
(Lisbon,P;London,GB)
6. TransportofsulphatedbileacidsbycanalicularABCG2
A.G.Blazquez,O.Briz,M.R.Romero,R.Rosales,P.Martinez-Becerra,
C.Ghanem,M.A.Serrano,J.J.G.Marin(Salamanca,E;BuenosAires,RA)
7. Thebileacidsandbilealcoholsofbirds:Evolutiongivesrisetoadazzling
varietyofstructures
L.R.Hagey(LaJolla,USA)
8. Theevolutionofbilesaltsinvertebrates:Newfindings
L.R.Hagey(LaJolla,USA)
9. Alteredbileacidmetabolisminchildhoodfunctionconstipation:Inactivationof
secretorybileacidsbysulfationinasubsetofpatients
A.F.Hofmann,V.Loening-Baucke,J.Lavine,L.R.Hagey,J.Steinbach,
C.Packard,T.Griffin,D.Chatfield(LaJolla,IowaCity,SanDiego,USA)
10. Increasedserum27-hydroxycholesterolconcentrationindicatesanactivationof
LXRαandanexcessofcholesterolinthehumanbody
A.Honda,T.Ikegami,M.Shirai,T.Hara,Y.Homma,Y.Matsuzaki
(Ami,Mito,Isehara,J)
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11. ACAT2andhumanhepaticcholesterolmetabolism:Identificationofimportant
gender-relateddifferences
Z.Y.Jiang,P.Parini,C.A.Einarsson,G.Eggertsen,M.A.Davis,S.-D.Zhang,
L.L.Rudel,T.-Q.Han,L.Eriksson(Huddinge,S;Winston-Salem,USA;
Shanghai,RC)
12. Isolation,identification,andchemicalsynthesisofnovelbileacidsinvertebrates
G.Kakiyama,T.Iida,A.Muto,N.Mano,J.Goto,L.R.Hagey,A.F.Hofmann
(Tokyo,Sendai,J;LaJolla,USA)
13. Hepaticcytochromep450oxidoreductaseknockoutmicehavealteredbile
formation
C.Kunne,D.R.deWaart,C.C.Paulusma,R.P.J.OudeElferink
(Amsterdam,NL)
14. Degradationof24S-hydroxycholesterolinmanisnotregulatedbyCyp7A1
D.Lütjohann,C.Knabe,T.Sudhop,K.vonBergmann(Bonn,D)
15. Metabolismofside-chainmodifiedursodeoxycholicacidinMdr2-/-mice
H.-U.Marschall,E.Halibasic,P.Fickert,T.Moustafa,M.Wagner,G.Zollner,
M.Trauner(Huddinge,S;Graz,A)
16. Analysisoflithocholicacid-taggedproteinsintheratliverbyusing
immunoaffinitycaptureandMALDI-TOFMS
S.Ikegawa,T.Yamamoto,K.Mitamura(Higashi-Osaka,J)
17. Mechanisticstudiesontheformationofacylglutathioneconjugateofbileacidin
ratliveranditsbiliaryexcretion
K.Mitamura,S.Watanabe,Y.Mitsumoto,T.Sakai,M.Sogabe,T.Wakamiya,
S.Ikegawa(Higashi-Osaka,J)
18. Structure-activityrelationshipsofAKR-typeoxidoreductasesinvolvedinbile
acidsynthesis
U.Oppermann,P.Lukacik,K.Guo,W.H.Lee,K.L.Kavanagh(Oxford,GB)
19. Unconjugatedbilesaltsshuttlethroughhepatocyteperoxisomesforglycineor
taurineconjugation
K.P.Rembacz,J.Woudenberg,E.Jonkers,H.Moshage,F.Stellaard,
K.N.Faber(Groningen,NL)
20. IncreasingexpressionofthecholesterolbindingSTARTdomainprotein,
StarD4,stimulatesratesofbileacidsynthesisandcholesterolesterformationin
primarymousehepatocytes
D.Rodriguez-Agudo,S.Ren,E.Wong,D.Marques,K.Redford,P.B.Hylemon,
G.Gil,W.M.Pandak(Richmond,USA)
21. Bileacidsynthesisinmanrelatestoplasmatriglycerides,genderandpresence
ofgallbladderbutnottoaging
C.Gälman,B.Angelin,B.Thorsson,A.Bröijersen,M.Rudling(Huddinge,S;
Kopavogur,IS)
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