Basic Methods in Antibody Production and Characterization

286 Pages·2000·5.4 MB·English

by  Gary C. Howard (Editor)

BASIC METHODS IN
Antibody Production
and
Characterization
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BA S I C M E T H O D S I N
Antibody Production
and
Characterization
Edited by
Gary C. Howard
Castro Valley, CA
Delia R. Bethell
BioSeparations, Inc.
Tucson, AZ
CRC Press
Boca Raton London New York Washington, D.C.
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Library of Congress Cataloging-in-Publication Data
Basic methods in antibody production and characterization / edited by Gary C. Howard
and Delia R. Bethell
p. cm.
Includes bibliographical references and index.
ISBN 0-8493-9445-7 (alk. paper)
1. Immunoglobulins—Laboratory manuals. I. Howard, Gary C. II. Bethell, Delia R.
QR186.7 .B37 2000
616.07′98—dc21 00-033729
CIP
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Preface
Antibodies have become indispensable tools for biomedical research. Their extraor-
dinary specificity of binding is matched by their ease of use and adaptability to many
experimental and now even some therapeutic applications.
Basic Methods in Antibody Production and Characterization is a cookbook of
methods for the production and use of antibodies. We have attempted to cover all
aspects of poloyclonal and monoclonal antibodies. Many of the procedures are well
established. Others (e.g., phage display) are quite new. Although we have sought to
present this material in a simple step-by-step manner, no methods book can cover
every experimental protocol. Each contributor has provided introductory material to
give a basic understanding of the methods and references to other applications of
his or her chapter’s particular technology.
The methods presented are more or less in chronological order. The most impor-
tant element in making antibodies is the ultimate purpose of the experiments. Time,
experience, expense, and the scientific questions to be answered will direct the choice
of methods for antibody production.
We want to thank each of the contributors to this volume. Without their profes-
sional knowledge and enthusiastic efforts, the book would not have been possible.
We also thank Yale Altman for his encouragement, guidance, and unflagging support
throughout the project.
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The Editors
Gary C. Howard, Ph.D., received a B.A. in zoology in 1971 and an M.S. in biology
from West Virginia University, Morgantown, and a Ph.D. in biological sciences from
Carnegie Mellon University, Pittsburgh, PA. After fellowships at Harvard and Johns
Hopkins, Dr. Howard spent 10 years with biotech companies. He edited Methods in
Nonradioactive Detection (Appleton & Lange, 1993) and, with W. E. Brown, is
currently preparing a volume on the practical aspects of advanced methods in protein
chemistry for CRC Press. Dr. Howard is a freelance editor and writer and a senior
scientific editor for the J. David Gladstone Institutes, a private biomedical research
institution affiliated with the University of California, San Francisco.
Delia R. Bethell, Ph.D., received a B.S. in biology from Newcomb College, Tulane
University, New Orleans, LA, in 1968, an M.S. in biology from University of Denver,
Denver, CO, and a Ph.D. in physiology at Hershey Medical Center, Pennsylvania
State University. Dr. Bethell has worked with the production of monoclonal anti-
bodies and their use in the area of in vitro diagnostics for a number of years. She
has served on the Executive Board of the Society for In Vitro Biology for the past
10 years, holding the office of President from 1998 to 2000. Dr. Bethell is currently
director of research with BioSeparations, Inc., in Tuscon, AZ.
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Contributors
Peter Amersdorfer, Ph.D. Nicholas R. Griffin, M.D.
Phylos Consultant Histopathologist
300 Putnam Avenue Histopathology Department
Cambridge, MA 02139 Airedale General Hospital
[email protected] Skipton Road near Keighley
Yorkshire BD20 6TD
Jory R. Baldridge, Ph.D. England
Ribi ImmunoChem Research, Inc.
553 Old Corvallis Road Neil M. Hand
Hamilton, MT 59840 Chief Medical Laboratory
Scientific Officer
Eric S. Bean, Ph.D. Histopathology Department
HTI Bio-Products University Hospital
P.O. Box 1319 Queen's Medical Centre
Ramona, CA 92065 Nottingham NG7 2UH
[email protected] England
[email protected]
Lee Bendickson
Department of Biochemistry, Kristi R. Harkins, Ph.D.
Biophysics and Molecular Biology Iowa State University
Iowa State University 1104 Molecular Biology Building
Ames, IA 50011 Ames, IA 50011
[email protected]
Delia R. Bethell, Ph.D.
BioSeparations, Inc. Mark D. Hirschel, Ph.D.
245 South Plumer Avenue, Suite 28 Cellex Biosciences
Tuscon, AZ 85719 8500 Evergreen Boulevard
[email protected] Coon Rapids, MN
Kathryn Elwell Gary C. Howard, Ph.D.
Genzyme Diagnostics, Inc. 17015 High Pine Way
1531 Industrial Road Castro Valley, CA 94546
San Carlos, CA 94070 [email protected]
[email protected]
Michael J. Lacy
Michael J. Gramer Ribi ImmunoChem Research, Inc.
Cellex Biosciences 553 Old Corvallis Road
8500 Evergreen Boulevard Hamilton, MT 59840
Minneapolis, MN 55433