Table Of ContentBASIC AND CLINICAL APPLICATIONS OF
FLOW CYTOMETRY
Developments in Oncology
53. M.P. Hacker, J.S. Lazo and T.R. Tritton (eds.): Organ Directed Toxicities of
Anticancer Drugs. 1988 ISBN 0-89838-356-0
54. M. Nicolini (ed.): Platinum and Other Metal Coordination Compounds in Cancer
Chemotherapy. 1988 ISBN 0-89838-358-7
55. J.R. Ryan and L.O. Baker (eds.): Recent Concepts in Sarcoma Treatment. 1988
ISBN 0-89838-376-5
56. M.A. Rich, J.C. Hager and D.M. Lopez (eds.): Breast Cancer. Scientific and
Clinical Aspects. 1988 ISBN 0-89838-387-0
57. B.A. Stoll (ed.): Women at High Risk to Breast Cancer. 1989
ISBN 0-89838-416-8
58. M.A. Rich, J.C. Hager and I. Keydar (eds.): Breast Cancer. Progress in
Biology, Clinical Management and Prevention. 1989 ISBN 0-7923-0507-8
59. P.I. Reed, M. Carboni, B.J. Johnston and S. Guadagni (eds.): New Trends in
Gastric Cancer. Background and Videosurgery. 1990 ISBN 0-7923-8917-4
60. H.K. Awwad: Radiation Oncology: Radiobiological and Physiological
Perspectives. The Boundary-Zone between Clinical Radiotherapy and
Fundamental Radiobiology and Physiology. 1990 ISBN 0-7923-0783-6
61. J.L. Evelhoch, W. Negendank, F.A. Valeriote and L.H. Baker (eds.): Magnetic
Resonance in Experimental and Clinical Oncology. 1990 ISBN 0-7923-0935-9
62. B.A. Stoll (ed.): Approaches to Breast Cancer Prevention. 1991
ISBN 0-7923-0995-2
63. M.J. Hill and A. Giacosa (eds.): Causation and Prevention of Human Cancer.
1991 ISBN 0-7923-1084-5
64. J.R.W. Masters (ed.): Human Cancer in Primary Culture. A Handbook. 1991
ISBN-O-7923-1088-8
65. N. Kobayashi, T. Akera and S. Mizutani (eds.): Childhood Leukemia. Present
Problems and Future Prospects. 1991 ISBN 0-7923-1138-8
66. P. Paoletti, K. Takakura, M.D. Walker, G. Butti and S. Pezzotta (eds.): Neuro-
oncology. 1991 ISBN 0-7923-1215-5
67. K.V. Honn, L.J. Marnett, S. Nigam and T. Walden Jr. (eds.): Eicosanoids and
Other Bioactive Lipids in Cancer and Radiation Injury. 1991
ISBN 0-7923-1303-8
68. F.A. Valeriote, T.H. Corbett and L.H. Baker (eds.): Cytotoxic Anticancer
Drugs: Models and Concepts for Drug Discovery and Development. 1992
ISBN 0-7923-1629-0
69. L. Dogliotti, A. Sapino and G. Bussolati (eds.): Breast Cancer. Biological and
Clinical Progress. 1992 ISBN 0-7923-1655-X
70. E. Benito, A. Giacosa and M.J. Hill (eds.): Public Education on Diet and
Cancer. 1992 ISBN 0-7923-8997-2
71. S. Nigam, K.V. Honn, L.J. Morvett and Th.L. Walden, Jr. (eds.): Eicosanoids
and Other Bioactive Lipids in Cancer, Inflammation and Radiation Injury. 1992
ISBN 0-7923-1870-6
72. F.H. Menko: Genetics of Colorectal Cancer for Clinical Practice. 1993
ISBN 0-7923-2100-6
73. R.P. Gallagher and J.M. Elwood (eds.): Epidemiological Aspects of Cutaneous
Malignant Melanoma. 1994 ISBN 0-7923-2740-3
KLUWER ACADEMIC PUBLISHERS-DORDRECHTIBOSTON/LONDON
BASIC AND CLINICAL APPLICATIONS OF
FLOW CYTOMETRY
Proceedings of the
24th Annual Detroit Cancer Symposium
Detroit, Michigan, USA - April 30, May 1 and 2, 1992
Edited by
Frederick A. Valeriote, Ph.D.
Alexander Nakeff, Ph.D.
Manuel Valdivieso, M.D.
Wayne State University
School of Medicine
Detroit, Michigan
....
"
Kluwer Academic Publishers
Boston/Dordrecht/London
Distributors for North America:
Kluwer Academic Publishers
101 Philip Drive
Assinippi Park
Norwell, Massachusetts 02061 USA
Distributors for all other countries:
Kluwer Academic Publishers Group
Distribution Centre
Post Office Box 322
3300 AH Dordrecht, THE NETHERLANDS
Library of Congress Cataloging-in-Publication Data
A C.I.P. Catalogue record for this book is available from
the Library of Congress.
ISBN-13: 978-1-4612-8534-2 e-ISBN-13: 978-1-4613-1253-6
DOl: 10.1007/978-1-4613-1253-6
Copyright ~ 1996 by Kluwer Academic Publishers
Softcover reprint of the hardcover 1st 1996
All rights reserved. No part of this publication may be reproduced, stored in
a retrieval system or transmitted in any form or by any means, mechanical,
photo-copying, recording, or otherwise, without the prior written permission of
the publisher, Kluwer Academic Publishers, 101 Philip Drive, Assinippi Park,
Norwell, Massachusetts 02061
Printed on acid-free paper.
CONTENTS
1. CYTOMETRY 2000: LASER PROBING THE FUTURE 1
Alexander Nakeff
2. ACTIVATION AND PROLIFERATION OF PURIFIED HEMATO
POIETIC PRECURSOR CELLS FROM HUMAN BONE MARROW 5
P. Lansdorp, W. Dragowska, and T. Thomas
3. FLOW CYTOMETRIC PROLIFERATIVE FRACTION ANALYSIS
IN SOLID TUMORS 13
Daniel Visscher, Susan M. Wykes, and
John D. Crissman
4. MITOGENIC AND NON-MITOGENIC INDUCTION OF LYMPHO
CYTIC INVASION: DUAL PARAMETER FLOW CYTOMETRIC
ANALYSIS 33
S. Ratner and D. Lichlyter
5. FLOW CYTOMETRIC MONITORING OF DRUG RESISTANCE IN
HUMAN SOLID TUMORS 49
Awtar Krishan, Cheppail Ramachandran, and
Antonieta Sauerteig
6. FLOW CYTOMETRY IN ONCOLOGY: SOME LESSONS FROM
HISTORY 65
Howard M. Shapiro
7. DNA FLOW CYTOMETRY APPLICATION TO CLINICAL TRIALS
IN BREAST CANCER 81
Lynn G. Dressler
8. PROCESSING OF SOLID TUMORS FOR DNA ANALYSIS BY
FLOW CYTOMETRY 95
Charles L. Hitchcock, John F. Ensley, and
Mark Zalupski
9. SJL MODEL FOR LYMPHOMA TREATMENT 125
M. Cankovic, T. Wrone-Smith, E. VanBuren,
and S. Lerman
10. GENE MAPPING AND MOLECULAR CYTOGENETICS USING
FLOW-SORTED CHROMOSOMES 143
N.P. Carter
11. THE CLINICAL POTENTIAL OF DNA CONTENT PARAMETERS
IN HUMAN PEDIATRIC AND ADULT SOLID TUMORS 159
Mark Zalupski and John F. Ensley
12. DIFFERENTIATION OF HUMAN B-CELL TUMORS: A PRE
CLINICAL MODEL FOR DIFFERENTIATION THERAPY 179
Ayad M. AI-Katib and Ramzi Mohammad
VI
13. PROBING IMMUNE RESPONSES: THE ROLE OF INTRA-
CELLULAR GLUTATHIONE 197
Mario Roederer and Leonard A. Herzenberg
14. DNA TOPOISOMERASE II POISONS AND THE CELL CYCLE 211
P.J. Smith
15. IN SITU HYBRIDIZATION OF CENTROMERIC DNA AS
VISUALIZED ON THE NUCLEAR MATRIX BY LASER CON
FOCAL CYTOMETRY 227
Kenneth J. Pienta
16. MEGAKARYOCYTOPOIESIS 2000 239
Alexander Nakeff
17. THE MOLECULAR BASIS FOR THE CONTROL OF MAMMALIAN
CELL GROWTH 251
Gerard I. Evan
18. ISOLATION OF RARE CELLS BY HIGH-SPEED FLOW CYTO
METRY AND HIGH-RESOLUTION CELL SORTING FOR SUBSE
QUENT MOLECULAR CHARACTERIZATION - APPLICATION IN
PRENATAL DIAGNOSIS, BREAST CANCER AND AUTOLOGOUS
BONE MARROW TRANSPLANTATION 271
James F. Leary, Donald Schmidt, Janet G.
Gram, Scott R. McLaughlin, Camille DallaTorre,
Stefan Burde, and Steven P. Ellis
LIST OF PARTICIPANTS
Ayad Al-Katib, M.D. John Ensley, M.D.
Division of Hematology and Division of Hematology and
Oncology Oncology
Wayne State University Wayne State University
P.O. Box 02188 P.O. Box 02188
Detroit, MI 48201 Detroit, MI 48201
Kenneth D. Bauer, Ph.D. Gerard I. Evan, ph.D.
McGaw Medical Center Imperial Cancer Research Fund
Wesley Pavilion, Room 540 Dominion House
250 E. Superior Street Bartholomew Close
Chicago, IL 60611 London EC1A 7BE
United Kingdom
G.J. Brakenhoff, Ph.D.
Department of Molecular Cell David Hedley, M.D., Ph.D.
Biology Department of Medicine and
University of Amsterdam Pathology
Plantage Muidergracht 14 Princess Margaret Hospital
NL-10l8 TV Amsterdam 500 Sherbourne Street
The Netherlands Toronto, Ontario
M4X 1K9 Canada
Nigel Carter, D.Phil.
Department of Pathology Charles Hitchcock, M.D., Ph.D.
University of Cambridge Department of Pathology
Tennis Court Road The Ohio State University
Cambridge CB1 1QP Columbus, Ohio 43210
United Kingdom Formerly Director of Flow
Cytometry Laboratory
L. Scott Cram, Ph.D. Department of Cellular
Life Sciences Division Pathology
LS-DO, MS M881 Armed Forces Institute of
Los Alamos National Laboratory Pathology
Los Alamos, New Mexico 87545 Washington, D.C. 20306
John Crissman, M.D. Joseph Kaplan, M.D.
Department of Pathology Children's Hospital of
Harper Hospital Michigan
3990 John R 3901 Beaubien
Detroit, MI 48201 Detroit, MI 48201
Lynn G. Dressler, M.A. Awtar Krishan, Ph.D.
Division of Hematology and Michigan Cancer Foundation
Oncology 110 E. Warren
University of North Carolina Detroit, MI 48202
at Chapel Hill
3009 Old Clinic Bldg., CB 7305 Peter M. Lansdorp, Ph.D.
Chapel Hill, NC 27599 Terry Fox Laboratory
B.C. Cancer Research Center
601 W. 10th Avenue
Vancouver, B.C.
V5Z 1L3 Canada
viii
James F. Leary, Ph.D. Melvin Schindler, Ph.D.
The University of Texas Michigan State University
Medical Branch at Galveston Department of Biochemistry
Department of Internal East Lansing, MI 48824
Medicine
Sealy & Smith Professional Howard M. Shapiro, M.D.
Bldg., Suite 724 283 Highland Avenue
Galveston, TX 77555 West Newton, MA 02165
Stephen Lerman, Ph.D. Paul J. Smith, Ph.D.
Department of M.R.C. Clinical Oncology Unit
Immunology/Microbiology The Medical School
Wayne State University Hills Road
550 E. Canfield Cambridge, CB2 2QH
Detroit, MI 48201 United Kingdom
Michael R. Loken, Ph.D. Carleton C. Stewart, Ph.D.
Bio Logics Consulting Roswell Park Memorial
29 San Juan Court Institute
Los Altos, CA 94022 666 Elm Street
Buffalo, New York 14263
Michael Long, Ph.D.
Department of Pathology D. Lansing Taylor, ph.D.
Children's Hospital of Department of Biological
Michigan Science
3901 Beaubien Carnegie Mellon University
Detroit, MI 48201 4400 5th Avenue
Pittsburgh, PA 15213
Alexander Nakeff, Ph.D.
Division of Hematology and Manuel Valdivieso, M.D.
Oncology Division of Hematology and
Wayne State University Oncology
P.O. Box 02188 Wayne State University
Detroit, MI 48201 P.O. Box 02188
Detroit, MI 48201
Kenneth Pienta, M.D., Ph.D.
Division of Hematology and Frederick Valeriote, Ph.D.
Oncology Division of Hematology and
Wayne State University Oncology
P.O. Box 02188 Wayne State University
Detroit, MI 48201 P.O. Box 02188
Detroit, MI 48201
Stuart Ratner, Ph.D.
Michigan Cancer Foundation Daniel Visscher, M.D.
110 E. Warren Department of Pathology
Detroit, MI 48202 Harper Hospital
3990 John R
Mario Roederer, Ph.D. Detroit, MI 48201
B007 Beckman Center
Department of Genetics James Watson, M.D., PH.D.
Stanford University Medical M.R.C. Clinical Oncology Unit
Center The Medical School, Hills Road
Stanford, CA 94305 Cambridge CB2 2QH
United Kingdom
IX
Mark Zalupski, M.D.
Division of Hematology and
Oncology
Wayne State University
P.o. Box 02188
Detroit, MI 48201
ACKNOWLEDGEMENTS
Generous support from the following donors were critical to
the success of this Symposium:
- Division of Hematology and Oncology, Wayne State University
- Harper Hospital
- Children's Hospital of Michigan
- AMAC, Inc.
- Becton-Dickinson Immunocytometry Systems
- Boehringer Mannheim Corporation
- Bristol-Myers U.S. Pharmaceutical and Nutritional Group
- Coulter Corporation
- Dako Corporation
- Glaxo Pharmaceuticals
- Immunex Corporation
- Meridian Instruments, Inc.
- Ortho Diagnostic Systems, Inc.
- Research & Diagnostic Systems, Inc.
- Sandoz Pharmaceuticals Corporation
- The Upjohn Company
The contents of the book were prepared for camera-ready offset
printing with typing, layout and editorial assistance by Claudia A.
Valeriote.
