Table Of ContentContributors to Volume 184
parentheses are in numbers Article gniwollof the names of .srotubirtnoc
snoitailiffA are current. listed
MARGIE ADAMS (37), Department of Biol- CHRISTINE L. BRAKEL (51), ENZO Bio-
ogy, University of California, San Diego, chem, Inc., New York, New 3100York 1
La Jolla, California 39029
MARK S. BROWER (51), Division of Hema-
PAUL ANZIANO (35), Department of Bio- tology/Oncology, Department of Medi-
chemistry, University of Texas Health cine and the Specialized Center for Re-
Sciences Center, Dallas, Texas 53257 search ni Thrombosis, ehT New York
HIROAKI ASOU (45), Department of Physiol- Hospital-Cornell Medical Center, New
ogy, School of Medicine, Keio University, York, New York 12001
Tokyo 160, Japan
J. WILLIAM BUCKIE (32), Department of
STRATIS AVRAMEAS (55), Immunocytochi- Anatomy, University of Cambridge, Cam-
mie, lnstitut Pasteur, 42757 Paris, Cedex bridge 3D 2BC ,Y England
,51 France
GYAN CHANDRA (7), Department of Bio-
ETIENNE-EMILE BAULIEU (30), Facultd de
chemistry, Research Glaxo Laboratories,
Mddecine, Universitd Paris XI, and IN-
Research Triangle Park, North Carolina
SERM U 33 Communications Hor-
90772
monales, H6pital de Bic~tre, 94275
Bicdtre Cedex, France HSHI-CHI CHANG (57), Department of Vet-
erinary Public Health, Faculty of Veteri-
EDWARD A. BAYER (2, 3, 4, 6, 8, 13, 14, 15,
nary Medicine, Hokkaido University,
18, 22, 23, 26, 31, 36, 47, 48, 54, 65, 71,
Sapporo 060, Japan
73), Department of Biophysics, The Weiz-
mann Institute of Science, Rehovot JASBIR CHAUHAN (10), Department of Bio-
,00167 Israel chemistry and Molecular Biology, Fac-
ulty of Medicine, University of Manitoba,
L. DAWSON BEALL (28), Department oflm-
Winnipeg, Manitoba R 3,3EW O Canada
munology, Otsuka America Pharmaceuti-
cal Co., Rockville, Maryland 05802 LING-MEI CHEN (77), Department of Foren-
STEPHAN BECK (72), MilliGen/Biosearch, sic Medicine, Shanghai Medical Univer-
Burlington, Massachusetts 30810 sity, Shanghai 200032, China
HAYA BEN-HUR (8, 23, 47, 48), Department GWEN V. CHILDS (44), Department of Anat-
of Biophysics, ehT Weizmann Institute of omy and Neurosciences, ehT University
Science, Rehovot ,00167 Israel of Texas Medical Branch, Galveston,
Texas 05577
MELVIN BERGER (74), Department of Pedi-
atrics, Case Western Reserve University MARY K. CONRAD (76), National Institute
School of Medicine, Cleveland, Ohio on Drug Abuse, Baltimore, Maryland
60144 42212
MELVIN L. BILLINGSLEY (53), Department EVERLY CONWAY DE MACARIO (58),
of Pharmacology, Pennsylvania State Wadsworth Center for Laboratories and
University College of Medicine, Hershey, Research, New York State Department of
Pennsylvania 33071 Health and School of Public Health,
CLAUDE BONNARD (75), NESTEC SA, Cen- State University of New York, Albany,
tre de Recherche Nestld, Vevey, CH-1800 New York 10221
Switzerland GEOFFREY M. W. COOK (32), Department of
xi
xii CONTRIBUTORS TO VOLUME 184
Anatomy, University of Cambridge, Cam- YLREBMIK GARRY (51), Division of Hema-
bridge CB2 3DY, England tology/Oncology, Department of Medi-
cine and the Specialized Center for Re-
ITRUMANHSdPK !TRUMANIHSKAD (10, 12),
search in Thrombosis, The New York
Department of Biochemistry and Molecu-
Hospital-Cornell Medical Center, New
lar Biology, Faculty of Medicine, Univer-
York, New York 12001
sity of Manitoba, Winnipeg, Manitoba
R3E OW3, Canada GULILAT GEBEYEHU (66), Life Technolo-
gies Incorporated, Gaithersburg, Mary-
SILENROC ED GROOT (33), Laboratory of
land 20877
Cell Biology and Histology, Cellular Im-
munology Group, University of Amster- REDNAXELA N. GLAZER (19), Division of
dam, Academic Medical Center, 1105 AZ Biochemistry and Molecular Biology, De-
Amsterdam, The Netherlands partment of Molecular and Cell Biology,
University of California, Berkeley, Cali-
DNOMYAR C. DUHAMEL (21), Bard Car-
fornia 02749
diosurgery Division, C.R. Bard Incorpo-
rated, Billerica, Massachusetts 12810 PAULA J. IKSWOBARG (34), Section of Bio-
chemistry, Brown University, Providence,
ANNE H. DUTTON (37), Department of Biol-
Rhode Island 21920
ogy, University of California, San Diego,
La Jolla, California 39029 JOHN G. GRAY (7), Department of Biochem-
istry, Glaxo Research Laboratories, Re-
ARAaRAB J. FENN (68), Department of Bio-
search Triangle Park, North Carolina
chemistry, Medical College of Wisconsin,
90772
Milwaukee, Wisconsin 53226
N. GREEN MICHAEL (5), Laboratory of Pro-
EVE FINKELSTEIN (49), Syva Co., Palo
tein Structure, National Institute for
Alto, California 30349
Medical Research, London NW7 IAA,
SECNARF M. FINN (27), Protein Research England
Laboratories and Department of Medi-
ERNEST V. GROMAN (22), Advanced Mag-
cine, University of Pittsburgh School of
netics Incorporated, Cambridge, Massa-
Medicine, Pittsburgh, Pennsylvania
chusetts 02138
16251
DRAHCIR FISHEL (35), Laboratory of Chro- NOBUO HASHIMOTO (57), Department of
Veterinary Public Health, Faculty of Vet-
mosome Biology, BRI-Basic Research
erinary Medicine, Hokkaido University,
Program, National Cancer Institute,
Sapporo 060, Japan
Frederick Cancer Research Facility,
Frederick, Maryland 10712 ELI HAZUM (29), Department of Endocri-
YNOHTNA C. FORSTER (69), Department of nology, Glaxo Research Laboratories,
Biology, Yale University, New Haven, Research Triangle Park, North Carolina
Connecticut 06520 90772
GLYN R. FOX (52), Department of Biology, YROGERG S. HEARD (11), Department of
University of Victoria, Victoria, British Neurology, Medical College of Virginia,
Columbia V8W 2Y2, Canada Virginia Commonwealth University,
Richmond, Virginia 23298
YELNATS C. RENHEORF (50), Department of
Biochemistry, Dartmouth Medical YHTOMIT M. HERMAN (68), Department of
School, Hanover, New Hampshire 65730 Biochemistry, Medical College of Wis-
consin, Milwaukee, Wisconsin 62235
ARABRAB FUDEM-GOLDIN (17), Department
of Molecular Pharmacology, Albert Ein- YAFFA HILLER (6), Department of Biophys-
stein College of Medicine, Bronx, New ics, The Weizmann Institute of Science,
York 16401 Rehovot 76100, Israel
CONTRIBUTORS TO VOLUME 184 xiii
KLAUS HOFMANN (27), Protein Research ERREIP-NAEJ LHUBNEHEARK (75), Swiss In-
Laboratories, University of Pittsburgh stitute for Experimental Research Cancer
School of Medicine, Pittsburgh, Pennsyl- and Institute of Biochemistry, University
vania 16251 of Lausanne, Epalinges, CH-1066 Switz-
PAUL HOROWITZ (25), Department of Bio- erland
chemistry, University of Texas Health HCIRLU (60), KROGER Department of -inilC
Science Center at San Antonio, San Anto- cal Chemistry and Pathobiochemistry,
nio, Texas 48287 Medical Faculty, University of Technol-
Su-MING Hsu (39), Department of Pathol- ogy, D-5100 Aachen, Federal Republic of
ogy and Laboratory Medicine, University Germany
of Texas Health Science Center at Hous-
WILLIAM J. LAROCHELLE (50), Laboratory
ton, Houston, Texas 52277
of Cellular and Molecular Biology, Na-
WALTER L. HURLEY (49), Department of tional Cancer Institute, National Insti-
Animal Sciences, University of Illinois, tutes of Health, Bethesda, Maryland
Urbana, Illinois 10816 298O2
KENNETH R. HUSKINS (64), E. I. UD Pont M. DANIEL LANE (20), Department of Bio-
De Nemours & Company, Inc., Medical
logical Chemistry, ehT Johns Hopkins
Products Department, Diagnostics Sys-
University, School of Medicine, Balti-
tems Research and Development Divi-
more, Maryland 50212
sion, Newark, Delaware 41791
MERLIN M. L. LEON(G5 2), Department of
HYMES JEANNE (11), Department of Human
Biology, University of Victoria, Victoria,
Genetics, Medical College of Virginia,
British Columbia, WBV 2112 Canada
Virginia Commonwealth University,
Richmond, Virginia 89232 COREY LEVENSON (67), Department of
Chemistry, Cetus Corporation, Emery-
KENNETH A. NOSBOCAJ (79), Laboratory of
ville, California 80649
Chemistry, National Institute of Diabe-
tes, Digestive, and Kidney Diseases, Na- ODED LIVNAH (9), Department of Struc-
tional Institutes of Health, Bethesda, tural Chemistry, The Weizmann Institute
Maryland 29802 of Science, Rehovot ,00167 Israel
TREBOR J. JOVELL (58), Wadsworth Center MATHEW M. S. LO (76), ICIAmericas, Inc.,
for Laboratories and Research, New York Wilmington, Delaware 79891
State Department of Health and School
OTREBLA J. L. MACARIO (58), Wadsworth
of Public Health, State University of New
Center for Laboratories and Research,
York, Albany, New York 10221
New York State Department of Health
LLADNAR L. KINCAID (53), Section on Im- and School of Public Health, State Uni-
munology, National Institute of Alcohol versity of New York, Albany, New York
Abuse and Alcoholism, Rockville, Mary- 10221
land 25802
SEMAJ L. MCINNES (69), Department of Bio-
DRANOEL KLEVAN (66), Life Technologies chemistry, University of Adelaide, Ade-
Incorporated, Gaithersburg, Maryland laide, South Australia Australia 5000,
77802
NYRHTAK E. MEIER (78), Department of
DLANOR A. IKSNAHOK (20), Department of Pharmacology, Howard Hughes Medical
Biochemistry, .rM Sinai School of Medi- Institute, University of Washington, Seat-
cine, New York, New York 92001 tle, Washington 81189
ENUTROF KOHEN (56), Department of Hor- DLANOD M. MOCK (24, 25), Department of
mone Research, ehT Weizmann Institute Pediatrics, University of lowa Hospitals
of Science, Rehovot ,00167 Israel and Clinics, Iowa City, Iowa 24225
XiV CONTRIBUTORS TO VOLUME 184
RANDALE. MORRIS (42), Department of KEVlN J. (64), REAGAN E. I. Du Pont De
Anatomy and Cell Biology, University of Nemours & Company, Inc., Medical
Cincinnati, College of Medicine, Cincin- Products Department, Diagnostics Sys-
nati, 7625Ohio 4 tems Research and Development Divi-
sion, Newark, Delaware 41791
LATA S. RAKRUREN (63), Laboratory of -uT
mor Cell Biology, National Cancer Insti- EDWARD S. RECTOR (12), Department of
tute, National Institutes of Health, Be- Immunology, Faculty of Medicine, Uni-
thesda, Maryland 29802 versity of Manitoba, Winnipeg, Manitoba
R3 E,3W O Canada
JEANNE E. NEUMANN (64), E. I. Du Pont
DRAR~IG (30), REDEUILH Facultd de M~de-
De Nemours & Company, Inc., Medical
cine, Universitd Paris XI, and INSERM
Products Department, Diagnostics Sys-
U 33 Communications Hormonales, Hb-
tems Research and Development Divi-
pital de Bic$tre, 94275 Bic$tre Cedex,
sion, Newark, Delaware 41791
France
(28), NEWMAN WALTER Department of Im- AvI (71), REISFELD Department of Biophys-
munology, Otsuka America Pharmaceuti- ics, The Weizmann Institute of Science,
cal Co., Rockville, Maryland 05802 Rehovot ,00167 Israel
IZHAK NIR (41), Department of Pathology, REDNAXELA RICH (35), Department of Biol-
University of Texas Health Science Cen- ogy, Massachusetts Institute of Technol-
ter at San Antonio, San Antonio, Texas ogy, Cambridge, Massachusetts 83120
48287 CIREDERF M. SDRAHCIR (1), Department of
DANIEL J. O'SHANNESSY (16), SmithKline Molecular Biophysics and Biochemistry,
Beecham Pharmaceutical, Research and Yale University, New Haven, Connecti-
Development, King of Prussia, Pennsyl- cut 11560
vania 60491 YERFFEJ M. GREBNEHTOR (22, 71), Depart-
ment of Biophysics, The Weizmann Insti-
EGROEG A. RRO (17), Department of Molec-
tute of Science, Rehovot ,00167 Israel
ular Pharmacology, Albert Einstein Col-
lege of Medicine, Bronx, New York 16401 ARNOLD E. RUOHO (78), Department of
Pharmacology, University of Wisconsin,
DAVID S. RETSAMREPAP (41), Department
School of Medicine, Madison, Wisconsin
of Pathology, University of Texas Health
60735
Science Center at San Antonio, San An-
ENIRAHTAC B. SAELINGER (42), Depart-
tonio, Texas 48287
ment of Microbiology and Molecular -eG
KEITH R. REKCAPYNNEP (53), Department netics, University of College oCincinnati f
of Anatomy, Medical College of Pennsyl- Medicine, Cincinnati, Ohio 76254
vania, Philadelphia, Pennsylvania 92191 RACHEL NOSREENHCS (62), Laboratory of
RICHARD M. PINO (43), Department of Developmental and Molecular Immunity,
Anatomy, Louisiana State University National Institute of Child Health and
Medical Center, New Orleans, Louisiana Human Development, National Institutes
21107 of Health, Bethesda, Maryland 29802
ARABRAB G. (41), SCHNEIDER Department
HPESOJ W. (53), POLLI Department of Phar-
of Pathology, University of Texas Health
macology, Pennsylvania State University
Science Center at San Antonio, San Anto-
College of Medicine, Hershey, Pennsyl-
nio, Texas 48287
vania 33071
REDNAXELA SCHWARZ (15), Institute of
ZAFAR I. (28), RANDHAWA Protein Chemis- Biotechnology 2, Nuclear Research Cen-
try Section, Otsuka America Pharmaceu- ter, D-5170 Julich, Federal Republic of
tical Co., Rockville, Maryland 05802 Germany
CONTRIBUTORS TO VOLUME 184 XV
CLAUDE OCCES (30), FaculN de M~decine, TREBOR H. (69), SYMONS Department of
Universitd Paris XI, and INSERUM 33 U Biochemistry, University of Adelaide, Ad-
Communications Hormonales, H3pital de elaide, South Australia 5000, Australia
Bic~tre, 94275 Bic~tre Cedex, France
RUHTRA J. IKSWOKTYS (38), Laboratory for
MING-CHuAN Sr~AO (77), Department of Cell and Molecular Biology, Division of
Biochemistry, Shanghai Medical Univer- Hematology and Oncology, New England
sity, Shanghai 200032, China Deaconess Hospital, Department of Med-
EDWARD L. (67), SHELDON Molecular De- icine, Harvard Medical School, Boston,
vices Corporation, Menlo Park, Califor- Massachusetts 51120
nia 52049
OUKI AMIlrSAKAT (57), Department of Vet-
JOHN E. (59, SHIVELY 60), Division of Im- erinary Public Health, Faculty of Veteri-
munology, Beckman Research Institute of nary Medicine, Hokkaido University,
the City of Hope, Duarte, California Sapporo 060, Japan
01019
ES~IR~HT KCNYNRET (55), Immunocytochi-
S. J. (37), SINGER Department of Biology, mie, lnstitut Pasteur, 75724 Paris, Cedex
University of California, San Diego, La ,51 France
Jolla, California 39029
WILLIE F. VANN (62), Laboratory of Bacte-
KERED C. (69), SgINGLE Department of Bio-
rial Polysaccharides, Center for Biologics
chemistry, University of Adelaide, Ade-
Evaluation and Research, Food and Drug
laide, South Australia 5000, Australia
Administration, Bethesda, Maryland
EHUD YKSLETUKS (36), Department of Pa- 298O2
thology, Sackler School of Medicine, Tel
Aviv University, Ramat Aviv, Israel LEAHPAR P. VlSCIDI (70), Eudowood Divi-
sion of Infectious Diseases, Department
(33), STmI~EMA FRANK Laboratory of Cell of Pediatrics, The Johns Hopkins Hospi-
Biology and Histology, Cellular Immunol-
tal, Baltimore, Maryland 50212
ogy Group, University of Amsterdam, Ac-
ademic Medical Center, 1105 AZ Amster- HPOTSIRHC RENEGAW (59, 60), Department
dam, The Netherlands of Clinical Chemistry and Pathobio-
chemistry, Medical Faculty, University of
NAITSIRHC J. REGRUBSARTS (56), Depart-
Technology, D-5100 Aachen, Federal Re-
ment of lnternal Medicine, University of
public of Germany
Liibeck, Liibeck D-2400, Federal Repub-
lic of Germany NAITSIRHC YERDNAW (15), Institute of Bio-
technology 2, Nuclear Research Center,
LUBERT (19), STRYER Department of Cell
D-5170 Julich, Federal Republic of Ger-
Biology, Stanford University School of
many
Medicine, Stanford, California 50349
JOEL L. NAMSSUS (9), Department of Struc- REGOR A. (40), WAgNKE Department of Pa-
tural Chemistry, The Weizmann Institute thology, Stanford University Medical
of Science, Rehovot 76100, Israel Center, Stanford, California 50349
KAZUO SUTOH (46), Department of Pure TREBOR NOSTAW (67), PCR Division, Cetus
and Applied Sciences, College o fArts and Corporation, Emeryville, California
Sciences, University of Tokyo, Komaba, 80649
Tokyo 153, Japan
JOHN A. WEHRLY (64), E. .1 Du Pont De
ANN SUTTON (62), Center for Biologics Nemours & Company, Inc., Imaging Sys-
Evaluation andR esearch, Food and Drug tems Department, Research and Develop-
Administration, Bethesda, Maryland ment Division, Brevard, North Carolina
29802 21782
xvi TO VOLUME CONTRIBUTORS 184
ECNERWAL M. (40), WEISS Division of Pa- (77), WOLD FINN Department of Biochemis-
thology, City of Hope National Medical try and Molecular Biology, The Univer-
Center, Duarte, California 50349 sity of Texas Medical School, Houston,
SEMAJ S. DAEHETIHW (21), Vector Labora- Texas 52277
tories, Incorporated, Burlingame, Cali- YRRAB (11), WOLF Departments of Human
fornia 01049 Genetics and Pediatrics, Medical College
LUCIA TREKCIW (59), Department of Clini- of Virginia, Virginia Commonwealth Uni-
cal Chemistry and Pathobiochemistry, versity, Richmond, Virginia 23298
Medical Faculty, University of Technol- NAJ RETSEEMMROW (33), Laboratory of Cell
ogy, D-5100 Aachen, Federal Republic of Biology and Histology, Cellular Immunol-
Germany ogy Group, University of Amsterdam, Ac-
MEIR KEHCLIW (2, 3, 4, 6, 8, ,31 ,41 ,51 ,81 ademic Medical Center, 1105 AZ Amster-
22, 23, 26, 31, 36, 47, 48, 54, 65, 71, 73), dam, The Netherlands
Department of Biophysics, The Weiz- TREBOR H. YOLKEN (61), The Johns
mann Institute of Science, Rehovot Hopkins University, School of Medicine,
,00167 Israel Baltimore, Maryland 50212
Preface
The development of avidin-biotin technology has unified and simpli-
fied a variety of technologies in which antibodies, lectins, hormones, and
other binders have been used to localize, isolate, and identify the corre-
sponding antigen, sugar moiety, receptor, etc. In all of these studies, in
order to detect the latter, the binder counterpart had to be modified in
some manner with a specific marker. In many cases, the preparation of
protein-protein conjugates were required. The use of such conjugates
resulted in a number of problems, often due to the modified physical and
chemical state of the binder, which could lead to an alteration in the
specificity and activity of the resultant conjugate.
The contribution of the avidin-biotin system is manifold. Foremost, of
course, is the remarkable affinity between the two biomolecules. An
added dimension of the system is the presence on egg white avidin (or its
bacterial relative, streptavidin) of four biotin-binding sites which often
generate an amplified signal. Moreover, avidin and streptavidin are ex-
ceptionally stable proteins, amenable to a wide variety of modifications
and conjugations which only negligibly disturb the activity or specificity
of their biotin-binding properties. Thus, protein-protein conjugates be-
tween the binder and marker are unnecessary, and only the biotinylation
of the binder is required. Subsequent interactions are accomplished using
the appropriate avidin- or streptavidin-associated probe.
In organizing this volume, we would have been delighted to have been
able to present definitive studies which could be used as a basis for the
design of new systems and applications. Unfortunately, this was not pos-
sible. In this explosive phase of the development of avidin-biotin technol-
ogy for different applications, we chose to present the currently estab-
lished uses from which (combined perhaps with a touch of imagination)
the experienced investigator should be able to extrapolate information
for the design of new and exciting uses for this complex.
There is much repetition, particularly with respect to the biotinylation
procedures and the preparation of avidin-associated probes. Since the
former is critical to most applications, we could not check the validity of
each procedure, but have provided a general detailed chapter describing
protocols and guidelines for the biotinylation of proteins based on our
own research. A definitive opinion on whether a spacer group is required
between the biotin moiety and the binder or probe is not given. In some
cases the spacer group is definitely required and an enhanced or stabilized
signal is received, but in others there appears to be no advantage to its
iivx
nlVX ECAFERP
use. Again, the properties of each system must be determined empiri-
cally. A clear stand has not been taken on whether an avidin-conjugated
probe should be used or whether a two-step approach (using either se-
quential treatment or preformed complexes of native avidin and biotinyla-
ted probe) should be employed. Again, each researcher must determine
experimentally which approach is most pertinent for the project involved.
We would like to acknowledge Mrs. Dvorah Ochert for her indispen-
sable assistance with the manuscripts, the correspondence, the editing,
typing, and retyping, all this in addition to her heavy departmental re-
sponsibilities. We also would like to thank our wives and families for their
patience and understanding during the periods of heightened activity.
And, of course, we are gratetfou l all of the contributors for their coopera-
tion in preparing their manuscripts for this volume.
MEIR WILCHEK
EDWARD A. BAYER
METHODS IN ENZYMOLOGY
VOLUME I. Preparation and Assay of Enzymes
Edited yb SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
VOLUME II. Preparation and Assay of Enzymes
Edited yb SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
VOLUME III. Preparation and Assay of Substrates
Edited yb SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
VOLUME IV. Special Techniques for the Enzymologist
Edited by SIDNEY P. COLOWICK DNA NATOH.A N KAPLAN
VOLUME V. Preparation and Assay of Enzymes
Edited yb SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
VOLUME VI. Preparation and Assay of Enzymes (Continued)
Preparation and Assay of Substrates
Special Techniques
Edited by SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
VOLUME VII. Cumulative Subject Index
Edited by SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
VOLUME VIII. Complex Carbohydrates
Edited yb ELIZABETH F. NEUFELD DNA VICTOR GRUBSNIG
VOLUME IX. Carbohydrate Metabolism
Edited by WILLIS A. WOOD
VOLUME X. Oxidation and Phosphorylation
Edited yb RONALD W. KOORBATSE DNA MAYNARD E. NAMLLUP
VOLUME XI. Enzyme Structure
Edited by C. H. W. HIRS
VOLUME XII. Nucleic Acids (Parts A and B)
Edited by LAWRENCE NAMSSORG DNA KIVIE EVADLOM
xix
XX N ISDOHTEM YGOLOMYZNE
EMULOV XlII. Citric Acid Cycle
Edited by J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited by J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited by DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
Edited by HTENNEK NITSUK
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited yb TREBREH ROBAT DNA WHITE CELIA ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited by DLANOD B. KCIMROCCM DNA LEUMEL D. THGIRW
VOLUME XIX. Proteolytic Enzymes
Edited by GERTRUDE E. PERLMANN AND LASZLO LORAND
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited by MAILLIW I. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
