Antibody Drug Discovery

486 Pages·2011·26.51 MB·English

by  Clive R. Wood

Antibody Drug
m
o
c
c. Discovery
ntifiy.
cieonl
worldsal use
w.on
wwpers
ded from 3/14. For
y Downloa79 on 10/0
ver6.1
o9
c1
Dis51.
Drug 146.1
dy by
o
b
nti
A

P743.9781848166288-tp.indd 1 10/11/11 3:45 PM
Molecular Medicine and Medicinal Chemistry
Book Series Editors: Professor Colin Fishwick (School of Chemistry, University
of Leeds, UK)
Dr Paul Ko Ferrigno and Professor Terence Rabbitts FRS,
FMedSci (Leeds Institute of Molecular Medicine,
St. James’s Hospital, UK)
Published:
MicroRNAs in Development and Cancer
edited by Frank J. Slack (Yale University, USA)
m
o
c
c. Merkel Cell Carcinoma: A Multidisciplinary Approach
ntifiy. edited by Vernon K. Sondak, Jane L. Messina, Jonathan S. Zager, and
worldscieal use onl Ronald C. DeConti (H Lee Moffitt Cancer Center & Research Institute, USA)
w.on DNA Deamination and the Immune System: AID in Health and Disease
wwpers edited by Sebastian Fugmann (National Institutes of Health, USA),
ded from 3/14. For MNianrai lPyna pDaivaazs (ilNioauti o(Rnoacl kInefsetiltluerte Us noifv Heresaitlyth, ,U USSAA)) and
y Downloa79 on 10/0 Aednitteibdo bdyy C Dlirvueg R D. Wiscooovde (rByayer HealthCare, Germany)
ver6.1
o9
c1
Dis51.
Drug 146.1 Forthcoming:
dy by Molecular Exploitation of Apoptosis Pathways in Prostate Cancer
o
b
nti by Natasha Kyprianou (University of Kentucky, USA)
A

Fluorine in Pharmaceutical and Medicinal Chemistry: From Biophysical
Aspects to Clinical Applications
edited by Véronique Gouverneur (University of Oxford, UK) and
Klaus Müller (F Hoffmann-La Roche AG, Switzerland)
XiaoLing - Antibody Drug Discovery.pmd 1 9/7/2011, 12:00 PM
me4 Molecular Medicine and
u
ol Medicinal Chemistry
V
m
o
c
c.
ntifiy. Antibody Drug
cieonl
worldsal use Discovery
w.on
wwpers
ded from 3/14. For
y Downloa79 on 10/0
ver6.1
o9
c1
Dis51.
Drug 146.1
dy by
o
b
nti
A
Clive R Wood

Bayer HealthCare, Germany
Editor
Imperial College Press
ICP
P743.9781848166288-tp.indd 2 10/11/11 3:45 PM
Published by
Imperial College Press
57 Shelton Street
Covent Garden
London WC2H 9HE
Distributed by
World Scientific Publishing Co. Pte. Ltd.
5 Toh Tuck Link, Singapore 596224
USA office: 27 Warren Street, Suite 401-402, Hackensack, NJ 07601
UK office: 57 Shelton Street, Covent Garden, London WC2H 9HE
m
o
c
c.
worldscientifial use only. BAr citaitsahlo Lguiber raercyo rCd afotar ltohgius ibnogo-kin i-sP auvbaliilcaabtlieo nfr oDma ttahe British Library.
w.on
wwpers
ded from 3/14. For MANolTeIcBulOarD MY eDdRicUinGe aDndIS MCOedVicEinRaYl Chemistry — Vol. 4
overy Downloa96.179 on 10/0 AesCylloeslc ptretyirmgroih ngntihsoc tw r o©e rsk em2nr0oev1wce2hdn a. b onTyrih c Iitamsol ,bpb ioeenro ciikalnu,lv doCeirnon tplgela edprg,ht eswo Pttihotrheceoroseuspoty fiw,n mrgi,ta tryee ncn ooprte drbimen girs eospiror aonnd fyur ocinemfdo tirhnme a aPntiuyo bnflo isrstmhoer roa.rg eb ya nadn yr emtreieavnasl,
c1
Dis51.
Drug 146.1
dy by
o
b
nti
A
For photocopying of material in this volume, please pay a copying fee through the Copyright
Clearance Center, Inc., 222 Rosewood Drive, Danvers, MA 01923, USA. In this case permission to
photocopy is not required from the publisher.
ISBN-13 978-1-84816-628-8
ISBN-10 1-84816-628-1
Typeset by Stallion Press
Email: [email protected]
Printed in Singapore.
XiaoLing - Antibody Drug Discovery.pmd 2 9/7/2011, 12:00 PM
b1153_FM.qxd 10/12/2011 3:31 PM Page v
b1153 Antibody Drug Discovery
Contents
m
o
c
c.
ntifiy.
worldscieal use onl CFoornetwriobrudtors vxiii
www.person John McCafferty
ded from 3/14. For PCrlievfea cRe.Wood xiii
y Downloa79 on 10/0 Chapter 1 OHluivmiearn Lizéagteiro ann odf JAosnét iWbo.dSaielsdanha 1
over96.1 Chapter 2 Selection and Screening ofAntibody Phage Display Libraries 25
c1
Dis51. David R.Buckler,Darren Schofield,Daniel J.Sexton,
Drug 146.1 David Lowe and Tristan J.Vaughan
dy by Chapter 3 Affinity Maturation Approaches for Antibody Lead 85
o
ntib Optimization
A David Lowe,Trevor Wilkinson and Tristan J.Vaughan

Chapter 4 Transgenic Mice Rearranging Human Antibodies 121
Sean Stevens
Chapter 5 Isotype Selection and Fc Engineering:Design and 147
Construction ofFit-for-Purpose Therapeutic
Antibodies
William R.Strohl
Chapter 6 Antibody Expression from Bacteriato Transgenic 221
Animals
Paul Stephens and Berni M.Sweeney
v
b1153_FM.qxd 10/12/2011 3:31 PM Page vi
b1153 Antibody Drug Discovery
vi Contents
Chapter 7 Current Trends in Antibody Purification and Lead 271
Selection
Jie Chen and Andrew Nixon
Chapter 8 Design and Application ofImmunoconjugates for 315
Cancer Therapy
SherifEl Sheikh,Hans-Georg Lerchen,Beate Müller-Tiemann
and Jörg Willuda
Chapter 9 Dual-Targeting Bispecific Antibodies as New Therapeutic 373
Modalities for Cancer
Zhenping Zhu
m
o
c.c Chapter 10 Antibody Fragments and AlternateProtein Scaffolds 409
ntifiy. Lioudmila Tchistiakova,William J.J.Finlay,
cieonl Stephane Olland,HelenDooleyand Davinder Gill
w.worldsonal use Editor Biography 441
wwpers Index 443
ded from 3/14. For
y Downloa79 on 10/0
ver6.1
o9
c1
Dis51.
Drug 146.1
dy by
o
b
nti
A
b1153_FM.qxd 10/12/2011 3:31 PM Page vii
b1153 Antibody Drug Discovery
Contributors
m
o
c
c.
ntifiy.
cieonl Foreword
worldsal use John McCafferty,University of Cambridge,Department of Biochemistry,Tennis
www.person Court Road,Cambridge,CB2 1QW,UK
ded from 3/14. For Editor
y Downloa79 on 10/0 C42li0v9e6 R W.Wupopoedr,taBl,ayGeerr HmeaanlythCare AG,GDD-Global Biologics,Aprather Weg 18a,
ver6.1
o9
Disc51.1 Authors
Drug 146.1 David R.Buckler, Dyax Corp., 300 Technology Square, Cambridge, MA 02139,
dy by USA
o
b
nti Jie Chen,Dyax Corp.,300 Technology Square,Cambridge,MA 02139,USA
A

Helen Dooley,Pfizer,Inc.,Foresterhill,Aberdeen,AB25 2ZS,UK
William J.J.Finlay,Pfizer,Inc.,Grange Castle Business Park,Clondalkin,Dublin
22,Ireland
Davinder Gill,Pfizer,Inc.,Global Biotherapeutic Technologies,87 Cambridgepark
Drive,Cambridge,MA 02140,USA
Olivier Léger,Merck Serono International S.A.,Protein Engineering & Antibody
Technologies, Geneva Research Center, Merck Serono International S.A., 9,
chemin des Mines,1202 Geneva,Switzerland
vii
b1153_FM.qxd 10/12/2011 3:31 PM Page viii
b1153 Antibody Drug Discovery
viii Contributors
Hans-Georg Lerchen,Bayer HealthCare AG,Medicinal Chemistry,Aprather Weg
18a,42096 Wuppertal,Germany
David Lowe,MedImmune Ltd.,Milstein Building,Granta Park,Cambridge,CB21
6GH,UK
Beate Müller-Tiemann, Bayer HealthCare AG, Global Biologics, Aprather Weg
18a,42096 Wuppertal,Germany
Andrew Nixon,Dyax Corp.,300 Technology Square,Cambridge,MA 02139,USA
Stephane Olland, Pfizer, Inc., Global Biotherapeutic Technologies, 87
m Cambridgepark Drive,Cambridge,MA 02140,USA
o
c
c.
ntifiy. José W. Saldanha, National Institute for Medical Research, Division of
cieonl Mathematical Biology,The Ridgeway,Mill Hill,London NW7 1AA,UK
worldsal use Darren Schofield,MedImmune Ltd.,Milstein Building,Granta Park,Cambridge,
w.on CB21 6GH,UK
wwpers
ded from 3/14. For DUSanAiel J. Sexton, Dyax Corp., 300 Technology Square, Cambridge, MA 02139,
y Downloa79 on 10/0 S42h0e9ri6f WElu Sphpeeikrtha,l,BGayeermr HaneyalthCare AG,Medicinal Chemistry,Aprather Weg 18a,
ver6.1 Paul Stephens, UCB Celltech, Discovery Biology, 216 Bath Road, Slough,
co19 Berkshire SL1 4EN,UK
Dis51.
Drug 146.1 Sean Stevens,Ablexis LLC,409 Illinois Street,San Francisco,CA 94158,USA
dy by William R.Strohl,Centocor R&D,Inc.,Biologics Research,145 King of Prussia
o
ntib Road,Radnor,PA 19087,USA
A

Berni M. Sweeney, UCB Celltech, Discovery Biology, 216 Bath Road, Slough,
Berkshire,SL1 4EN,UK
Lioudmila Tchistiakova, Pfizer, Inc., Global Biotherapeutic Technologies, 87
Cambridgepark Drive,Cambridge,MA 02140,USA
Tristan J. Vaughan, MedImmune Ltd., Milstein Building, Granta Park,
Cambridge,CB21 6GH,UK
Trevor Wilkinson,MedImmune Ltd.,Milstein Building,Granta Park,Cambridge,
CB21 6GH,UK
b1153_FM.qxd 10/12/2011 3:31 PM Page ix
b1153 Antibody Drug Discovery
Contributors ix
Jörg Willuda, Bayer HealthCare AG, Therapeutic Research Group Oncology,
Muellerstrasse 178,13353 Berlin,Germany
Zhenping Zhu, Kadman Pharmaceuticals, 450 East 29th Street, New York, NY
10016,USA
m
o
c
c.
ntifiy.
cieonl
worldsal use
w.on
wwpers
ded from 3/14. For
y Downloa79 on 10/0
ver6.1
o9
c1
Dis51.
Drug 146.1
dy by
o
b
nti
A
b1153_FM.qxd 10/12/2011 3:31 PM Page x
b1153 Antibody Drug Discovery
TThhiiss ppaaggee iinntteennttiioonnaallllyy lleefftt bbllaannkk
m
o
c
c.
ntifiy.
cieonl
worldsal use
w.on
wwpers
ded from 3/14. For
y Downloa79 on 10/0
ver6.1
o9
c1
Dis51.
Drug 146.1
dy by
o
b
nti
A