Annual report : National Institute of Diabetes and Digestive and Kidney Diseases

532 Pages·1991·18.4 MB·English

by  National Institute of Diabetes and Digestive and Kidney Diseases (U.S.)

AUG
BLDG 10, 10CENTER DR.
8ETHESDA, MD 20892-1150

NATIONAL INSTITUTE OF
DIABETES AND DIGESTIVE
AND KIDNEY DISEASES
ANNUAL REPORTS
DIVISION OF INTRAMURAL
RESEARCH
October 1, 1990 to September 30, 1991
?/
TABLE OF CONTENTS
PREFACE
Dr. Phillip Gorden, Director I
DIVISION OF INTRAMURAL RESEARCH
Dr. Allen Spiegel, Director
Dr. Edward Steers, Deputy Director
PROJECT REPORTS
MATHEMATICAL RESEARCH BRANCH
Summary 1
Mathematical formulations and analysis relevant to
experimental neurophysiology 9
Mathematical description of substrate transport in
capillary-tissue structures 10
Mathematical description of cellular neuroelectric
signal transmission 11
Electrical and chemical oscillations in coupled
eel1 systems 12
LABORATORY OF CELLULAR AND DEVELOPMENT BIOLOGY
Summary 13
Protein nucleic acid interactions: chromatin
structure and function 22
Study of ribonuclease and its inhibitor from
bacillus amyloliquefaciens 23
Studies of folic acid (dihydrofolate reductase) 24
Synthesis and transport of lipoprotein and hepatic
lipases in tissues and cells 25
Ultrastructural immunocytochemistry of lipid
metabolism in cultured cells and tissues 26
Large-scale processing of biological material 27
Regulation of developmental gene expression 28
Regulation of adipocyte metabolism 29
Control of gene expression in early mammalian
development 30
Chromatin structure in regulation of mammalian gene
expression 31

§ II
LABORATORY OF BIOCHEMISTRY AND METABOLISM
Summary 32
The role of the carbohydrate moiety of glycoprotein
in cellular activity 39
Enzymatic basis of detoxication 40
Polysaccharides in morphogenesis 41
Thermodynamic and kinetic studies of protein
structure and enzymic mechanisms 42
The role of the nuclear envelope in intracellular
protein sorting 43
Tissue specific and hormone regulated gene
expression 44
Inherited Disorders of Lysosomal Function 45
Electrochemical ion gradients as a mechanism of
cellular message transmission 46
Cell regulation by Hormones, Growth Factors,
Autoantibodies, and Oncogenes 47
Endocytosis, secretion and compartmentalization in
mutant CHO eelIs 48
The role of intracellular traffic in HIV infection 49
Cell specific activity of elements within the HIV-
LTR 50
Direct measurement of forces between membranes or
macromolecules 51
Physics of ionic channels and other proteins with
aqueous cavities 52
Structure and physical properties of DNA and DNA -
protein complexes 53
Histamine release on a hydration of Granule matrices 54
Cell-cell fusion due to influenza hemagglutinin 55
LABORATORY OF CHEMISTRY
Summary 56
Reactions and immunochemistry of carbohydrates 57
Analogues of thyrotropin - releasing hormone 58
Stereopopulation control in drug delivery and enzyme
simulation 59
Chemistry of bioimidazoles 60
Halogenated biogenic amines in biochemistry and
pharmacology 61
Significance of ligand tautomerism in biorecognition 62
Functionalized cogeners of bioactive compounds 63
Prosthetic groups for radiolabeling of
functionalized drugs and peptides 64
Development of drugs acting at adenosine receptors 65
Aldose reductase inhibitors 66
Novel amino acids for conformational and stereochemical
constraints in peptides 67
Florinated analogue of bioactive peptides 68