Table Of ContentANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS
Volume 13
JAACT 2002 FUCHU Organizing Committee
Meeting Chairperson: K. Yagasaki (Japan)
Meeting Secretaries: Y. Miura (Japan), M. Hattori (Japan), Y. Nomura (Japan)
Organizing Boards:
M. Al-Rubeai (UK) H. Anazawa (Japan) S. Asahi (Japan)
D. W. Barnes (USA) M. Carrondo (Portugal) D. Ejima (Japan)
A. Enomoto (Japan) Y. Fuke (Japan) Y. Fukui (Japan)
K. Furukawa (Japan) S. Hachimura (Japan) S. Hashizume (Japan)
T. Hisatsune (Japan) H. Hoshi (Japan) S. Iijima (Japan)
K. Ikura (Japan) D. W. Jayme (USA) T. Kadowaki (Japan)
M. Kamei (Japan) Y. Katakura (Japan) Y. Kitagawa (Japan)
K. Konstantinov (USA) T. Matsuda (Japan) O.-W. Merten (France)
M. Nagao (Japan) N. Nakamichi (Japan) H. Nakano (Japan)
G. Schmid (Switzerland) M. Shimizu (Japan) H. Shinmoto (Japan)
K. Shinohara (Japan) S. Shirahata (Japan) T. Suzuki (Japan)
M. Tanokura (Japan) M. Totsuka (Japan) E. Tsao (USA)
H. Tsumura (Japan) J. Wérenne (Belgium) F. Wurm (Switzerland)
M. Maeda-Yamamoto (Japan) M. Yokota (Japan)
Advisory Boards:
M. Aizawa (Japan) S. Arai (Japan) T. Fujimori (Japan)
K. Funatsu (Japan) S. Kaminogawa (Japan) K. Nagai (Japan)
T. Nishiyama (Japan) H. Ohigashi (Japan) T. Ohno (Japan)
H. Ohmura (Japan) T. Osawa (Japan) R. Sasaki (Japan)
G. H. Sato (USA)
Animal Cell Technology:
Basic & Applied Aspects
Volume 13
Proceedings of the Fifteenth Annual Meeting of the
Japanese Association for Animal Cell Technology (JAACT),
Fuchu, Japan, November 11–15, 2002
Edited by
KAZUMI YAGASAKI
YUTAKA MIURA
MAKOTO HATORI
and
YOSHIHIRO NOMURA
Department of Applied Biological Science,
Tokyo Noko University,
Tokyo, Japan
SPRINGER-SCIENCE+BUSINESS MEDIA, B.V.
A C.I.P. Catalogue record for this book is available from the Library of Congress.
ISBN 978-90-481-6557-5 ISBN 978-94-017-0726-8 (eBook)
DOI 10.1007/978-94-017-0726-8
Printed on acid-free paper
All Rights Reserved
©2003 Springer Science+Business Media Dordrecht
Originally published by Kluwer Academic Publishers in 2003
No part of this work may be reproduced, stored in a retrieval system, or transmitted
in any form or by any means, electronic, mechanical, photocopying, microfilming, recording
or otherwise, without written permission from the Publisher, with the exception
of any material supplied specifically for the purpose of being entered
and executed on a computer system, for exclusive use by the purchaser of the work.
Contents
Preface ......................................................................................................................... xiii
Plenary Lecture
Recombinant expression of human collagens
J. Myllyharju ................................................................................................... 1
Symposium
Recent Advances in the Materials for Reconstructive
Therapy and Tissue Engineering
Expression profiling of parietal endoderm cells and development of systems for large
scale production of laminins
Y. Hayashi, S. Futaki, K. Yagi, H. Bono, Y. Hayashizaki, Y. Okazaki, K.
Sekiguchi ......................................................................................................... 7
Synthetic peptides from laminin for tissue engineering
M. Mochizuki, M. Nomizu ............................................................................. 13
A novel technology for conversion of in vivo tissue architecture into a three-dimensional
in vitro culture mode.
r.
Takezawa ................................................................................................... 19
Construction of liver model with genetically engineered human HepG2 cells
r.
Omasa, S. Enosawa ................................................................................... 25
Structural Biology and Signal Transduction of Animal Cells
Determinants of ligand specificity in groups I and IV WW domains
Y. Kato, M. Ito, K. Kawai, K. Nagata, M. Tanokura ...................................... 31
Role of the WW domain in signaling and disease
M. Sudol .................................................................................................... 37
Structural biology of signal transduction mediated by Grb2
F. Inagaki ...................................................................................................... 43
v
vi
Cell Regulatory Functions of Food Factors and Natural Resources with Special
Reference to Health Promotion and Disease Prevention
Modulation of leukocyte function by food factors
M. Yamazaki, H. Ueda ................................................................................... 49
Anticancer effects of lignans
N. Saarinen, S. Makela, R. Santti .................................................................. 55
Effect of sulfur-containing active principles in garlic on the physiological function of
hepatocytes
L. y. Sheen ..................................................................................................... 59
Suppression of free radical-induced mutation of animal cell genes by food factors
AMurakami, H.-w. Kim, M. V. Williams, H. Ohigashi ................................. 65
Glycoengineering in Animal Cell Technology
Chemoenzymatic approaches for the preparation of oligosaccharides and glycopeptides
K. Ajisaka ...................................................................................................... 71
ESACT Special Lecture
Production of Biologicals
Cell growth arrest by nuc1eotides as a tool for improved production of
biopharmaceuticals
A. V. Carvalhal, s. sA Santos, A Coroadinha, I. Marcelino,
M. J. T. Carrondo .......................................................................................... 77
Cell Culture Engineering
Transient gene expression: A novel mammalian cell-based technology for recombinant
protein production
F. M. Wurm, L. Baldi, J. P. Girard, J. Lindell, F. Grosjean, N. Muller,
R. Jacquet, J. Wright, A Mason, D. Hacker, M. Dejesus, S. Picasso,
P. Batard, M. Jordan ..................................................................................... 83
Gene Therapy
Optimization of the production of retroviral vectors: Influences of the sugar source
A Geny-Fiamma, L. Millot, C. Rocca, O. Danos, O.-w. Merten .................. 89
Session
Cell Culture Engineering
Effects of glucose and CO concentrations on CRO cell physiology.
2
S. Takuma, C. Hirashima, J. M. Piret ........................................................... 99
vii
Suppression of glutamate-induced neural cell death by electrolyzed-reduced water
T. Kashiwagi, T. Hamasaki, M. Takaki, S. Kabayama, K. Teruya, Y. Katakura,
K. Otuba, S. Marisawa, S. Shirahata .......................................................... 105
Development of apoptosis-resistant dihydrofolate reductase-defficient chinese hamster
ovary cell line
S. K. Lee, G M. Lee .................................................................................... 111
High expression of human monoclonal antibodies in CHO cells selected using a
cycloheximide resistance marker
M. Tsukahara, E. Misawa, K. Kuroda, S. Oguchi, H. Yajima,
K. Kanda, H. Tsumura ................................................................................... 117
Development of a serum-free medium supplemented with hydrolysate for the suspension
culture of recombinant CHO cells producing human thrombopoietin
Y. H. Sung, G M. Lee .................................................................................. 121
Effect of growth factors and ascorbic acid 2-phosphate on liver specific functions of
porcine hepatocytes
A. Okamura, C. Ita, T. Tashie, Y. Shiba, D. Yang, M. Kadama ................... 127
Culture of porcine hepatocytes using radial flow bioreactor system
Y. Shiba, T. Kayama, C. Wang, Q. Zhang, A. Okamura, S. Aaki,
T. Mukaiyama, D. Yang, M. Kadama .......................................................... 133
The role of glutamine in hybridoma cell culture
H. Matsuaka, K. Hara, K. Nemata, T. Takeda ............................................. 139
Protective action of reduced water against alloxan-induced cytotoxicity
in pancreatic B -cell
Y. Li, T. Nishimura, K. Teruya, Y. Katakura, S. Kabayama, K. Otsuba,
S. Marisawa, Y. Ishii, Z. Gadek, S. Shirahata ............................................. 145
Effect of doxycycline-regulated chaperone expression on recombinant protein
productivity in CHO cells
S. 0. Hwang, J. Y. Chung, G M. Lee .......................................................... 151
Biphasic culture strategy based on hyperosmotic pressure for improved humanized
antibody production in chinese hamster ovary cell culture
M. S. Kim, N. S. Kim, Y. H. Sung, G M. Lee ............................................... 157
Effect of low culture temperature on foreign protein production
in recombinant CHO cells
S. K. Yaan, S. H. Kim, G M. Lee ................................................................. 163
Control of temperature and pH enhances human monoclonal antibody production in
CHO cell culture
S. Oguchi, H. Sa ita, M. Tsukahara, H. Tsumura ......................................... 169
viii
Effects of pluronic F68 on t-PA producing chinese hamster ovary (CHO) cells under
agitating and static conditions
K. Sato, V. Hendrick, J. Werenne J., R. Egashira ........................................ 173
Structure and function of laminin a4 G domain
R. Narita, H. Yamashita, R. Tajima Y. Kitagawa ......................................... 179
Introduction of Apoptosis-resistant genes to hybridoma cells and their influence
N. Sakai, S. Terada, M. Miki E. Suzuki ....................................................... 185
Production of Biologicals
An engineering method to high yielding production of recombinant
anti-HIV monoclonal antibody using generic multi feed fermentation
K. Kawatsu, Y. Ushio, K. Tsukiguma, Y. Ishikawa, H. Yokote ...................... 191
Proliferation and monoclonal antibody production of hybridoma cells immobilized on
non-woven polyester fabric discs
E. Aslankaraoglu, M. Gumusderelioglu, S. Yilmaz, N. Bingol .................... 197
Mass-production in yeast of laminins as the material of reconstructive therapy
K. Mizuno, T. Kadowaki, Y. Kitagawa ........................................................ 203
Functional Cell Lines
Effects of carvacrol on a human non-small cell lung cancer (NSCLC) cell line, A549
A. T. Koparal, M. Zeytinoglu ....................................................................... 207
Establishment of dendritic cell line from caspase-l-defficient mice
B. Nowak, H. Kitani, K. Eshima, K. Kuida, H. Tsutsui, N. M. Tsuji ............ 213
Isolation and characterization of fetal bovine cells derived from liver and bone marrow
T. Takenouchi, T. Takezawa ......................................................................... 219
Artificial selection of high proliferating and apoptosis-resistant NSO cells for industrial
production systems
D. Chau, M. Al-Rubeai ............................................................................... 225
Immunologicals, Monoclonal Antibodies, and Vaccines
Endothelial cells culture and its use to produce a vaccine against rickettsiae
V. Hedrick, N. Kagye, Y. Kamba, S. Simon, A. -L. Thinsy,
T. Marique, J. Werenne ............................................................................... 233
Monocytes suppress immune responses of peripheral blood lymphocytes: Possible
implication of immature dendritic cells
M. Yamashita, Y. Katakura, S. Matsumoto, T. Tamura,
K. Teruya, S. Shirahata ............................................................................... 237
ix
Establishment and analysis of germfree T cell receptor transgenic mice
M. Fujioka, S. Hachimura, A. Hosono, R. Nakamura, K. Hirayama,
K. Itoh, S. Kaminogawa .............................................................................. 243
Development of a novel process to produce a veterinary rabies vaccine in BHK-21 cells
grown on microcarriers in a 20 liter bioreactor
S. Rourou, S. Majoul, H. Loukil and H.Kallel ............................................. 249
Epitope-specific suppression of immune response against /3 -lactoglobulin induced by
oral administration of cow's milk with heat treatments
A. Enomoto, S. Koyahara, H. Harada, S. Komine ...................................... 255
Production of anti-peptide antibody by in vitro immunization
T. Tamura, Y. Katakura, M. Yamashita, S. Matsumoto,
K. Teruya, S. Shirahata ............................................................................... 261
Generation of mouse-human hybridomas secreting antibodies against peanut allergens
H. Shinmoto, T. Takahashi, K. Yamagishi, T. Kimura,
M. Suzuki, S. J. Maleki ................................................................................ 267
Reduced immunogenicity of /3 -lactoglobulin by conjugation with alginic acid
oligosaccharide
M. Hattori, S. Miyakawa, Y. Ohama, H. Kawamura,
T. Yoshida, K. Takahashi ............................................................................. 273
Transplantation, Artificial Organs, and Organ Substitutes
Evaluation of the immuno-protective effects of the new-type of bags using ELISA-and
FACS-analysis
M.S. Rahman, Y. Banu, A. Matsuoka, A. Ichikawa, M. Sakai,
H. Ikeda, T. Tsuchiya ................................................................................... 277
Effect of silk protein sericin on hepatoblastoma HepG2 cells
T. Kumagai, K. Takemura, S. Terada, A. Ogawa, M. Miki,
M. Sasaki, H. yamada ................................................................................. 281
Isolation of human mesenchymal stem cell-like fibroblast from adipose tissue for
reconstructi ve therapy
T. Miyazaki, K. Toriyama, S. Torii, Y. Kitagawa ......................................... 287
Change of the cellular function by connexin gene transfection in a hepatoma cell line
J. Yang, A. Ichikawa, T. Tsuchiya ................................................................ 293
Transgenic Animals
Production of pharmaceutical proteins by transgenic avian
A. Hotta, K. Ono, S. Mizuarai, K. Yamaguchi, K. Nishijima,
M. Kamihira, S. Iijima ................................................................................. 299
