Agrobacterium Protocols: Volume 2

372 Pages·2015·12.546 MB·English

by  Kan Wang (eds.)

Methods in
Molecular Biology 1224
Kan Wang Editor
Agrobacterium
Protocols
Volume 2
Third Edition
M M B
ETHODS IN OLECULAR IOLOGY
Series Editor
John M. Walker
School of Life and Medical Sciences
University of Hertfordshire
Hat fi eld, Hertfordshire, AL10 9AB, UK
For further volumes:
h ttp://www.springer.com/series/7651

Agrobacterium Protocols
Volume 2
Third Edition
Edited by
Kan Wang
Center for Plant Transformation, Plant Sciences Institute, and Department of Agronomy,
Iowa State University, Ames, IA, USA
Editor
Kan W ang
Center for Plant Transformation
Plant Sciences Institute
Department of Agronomy
Iowa State University
Ames , I A , USA
ISSN 1064-3745 ISSN 1940-6029 (electronic)
ISBN 978-1-4939-1657-3 ISBN 978-1-4939-1658-0 (eBook)
DOI 10.1007/978-1-4939-1658-0
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Dedication
To Marc Van Montagu and Jeff Schell (1935–2003), my Ph.D. mentors, for their inspira-
tion and encouragement.

Prefa ce
Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as
a pathogen causing the plant crown gall disease. Unlike many other pathogens, A grobacterium
is able to deliver DNA to plant cells and permanently alter the plant genome. The discovery of
this unique feature has provided plant scientists with a powerful tool to genetically transform
plants for both basic research purposes and for agricultural advancement.
The fi rst transgenic plants were reported a little over 30 years ago in 1983 by three
independent research groups. Using disarmed Agrobacterium vectors, these groups pro-
duced antibiotic-resistant transgenic tobacco Nicotiana tobaccum (Herrera-Estralla et al.,
1983, Nature 303: 209), N icotiana plumbaginifolia (Bevan et al., 1983, Nature 304: 184),
and petunia ( Petunia hybrid , Fraley et al., 1983, Proceedings of the National Academy of
Sciences 80: 4803). The three scientists who led the landmark work, Marc Van Montagu,
Mary-Dell Chilton, and Robert Fraley, were the laureates for the 2013 World Food Prize
( h ttp://www.worldfoodprize.org/en/laureates/2013_laureates/#StatementAchievem
ent ) . As the statement of achievement of the World Food Prize says, “… each conducted
groundbreaking molecular research on how a plant bacterium could be adapted as a tool to
insert genes from another organism into plant cells, which could produce new genetic lines
with highly favorable traits.”
While other methods such as biolistic gun, electroporation or polyethylene glycol can
also be used for introducing DNA molecules into plant cells, the Agrobacterium -mediated
transformation method remains the method of choices for most plant species in many labo-
ratories due to its effi ciency and its propensity to generate single or a low copy number of
integrated transgenes with defi ned ends.
When the fi rst edition of A grobacterium Protocols was published in 1995, exactly 20
years ago, only a handful of plants could be routinely transformed using Agrobacterium .
The second edition, which was published in 2006, collected transformation protocols for
59 plant species. In this third edition, we have updated protocols for 32 plant species from
the second edition and added protocols for 17 new species. Together with the fi rst and
second editions, these two new volumes offer A grobacterium -mediated genetic transforma-
tion protocols for a total of 76 plant species.
The third edition of Agrobacterium Protocols contains 57 chapters (two volumes)
divided into 9 parts. This edition emphasizes on agricultural crops or plant species with
economic values. For a number of important plants such as rice, barley, wheat, and citrus,
multiple protocols using different starting plant materials for transformation are included.
Like the second edition, plants are grouped according to their practical uses rather than
their botanical classifi cations.
Agrobacterium Protocols provides a benchtop manual for tested protocols involving
Agrobacterium -mediated transformation. All chapters are written in the same format as that
used in the Methods in Molecular Biology series. Each chapter is contributed by authors
who are leaders or veterans in their respective areas. The “Abstract” and “Introduction”
sections provide outlines of protocols, the rationale for selection of particular target tissues,
and information regarding overall transformation effi ciency. The “Materials” section lists
the host materials, Agrobacterium strains and vectors, stock solutions, media, and other
vii
viii Preface
supplies necessary for carrying out these transformation experiments. The “Methods” section
is the core of each chapter. It provides a step-by-step description of the entire transforma-
tion procedure from the preparation of starting materials to the harvest of transgenic plants.
To ensure the reproducibility of each protocol, the “Notes” section lists possible pitfalls in
the protocol and suggests alternative materials or methods for generating transgenic plants.
Typically, most laboratories only work on one or a few plant species. Each laboratory or
individual researcher has his or her own favorite variation or modifi cation of any given plant
transformation protocol. The protocols presented in this edition represent the most effi -
cient methods used in the laboratories of the contributors. They are by no means the only
methods for successful transformation of your plant of interest.
The broad range of target tissue selection and in vitro culture procedures indicate the
complexity in plant transformation. It is the intention of this book to facilitate the transfer
of this rapidly developing technology to all researchers for use in both fundamental and
applied biology. I take this opportunity to thank all my colleagues whose time and effort
made this edition possible. Special thanks go to my family for their unconditional love and
support during the process of editing this book.
Ames, IA, USA Kan W ang
Contents
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
PART I INDUSTRIAL PLANTS
1 Brassica rapa. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Tom L awrenson, C assandra G oldsack, L ars Ostergaard,
and Penny A . C. H undleby née S parrow
2 Cotton (Gossypium hirsutum L.) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1
Keerti S . R athore, LeAnne M . C ampbell, S hanna Sherwood,
and Eugenia N unes
3 J atropha (Jatropha curcas L.). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Devendra Kumar Maravi, P urabi M azumdar, Shamsher Alam,
Vaibhav V. G oud, and Lingaraj S ahoo
4 S esame (Sesamum indicum L.) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 7
Sonia Kapoor, Sanjay S . P armar, M anju Y adav, D arshna C haudhary,
Manish S ainger, Ranjana J aiwal, and Pawan K . Jaiwal
5 S unflower (Helianthus annuus L.). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Laura M. R adonic, D alia M . L ewi, N ilda E. L ópez, H. E steban Hopp,
Alejandro S . Escandón, and M arisa L ópez Bilbao
PART II ROOT PLANTS
6 Carrot (Daucus carota L.). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Owen S. D . W ally and Zamir K. P unja
7 C assava (Manihot esculenta Crantz). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Simon E. Bull
8 P otato (Solanum tuberosum L.). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Venkateswari J . C hetty, J avier N arváez-Vásquez,
and M artha L . Orozco-Cárdenas
9 T aro (Colocasia esculenta (L.) Schott) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 7
Xiaoling He, S usan C. M iyasaka, M aureen M. M . Fitch, and Yun J . Zhu
PART III NUTS AND FRUITS
10 Apricot (Prunus armeniaca L.). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
César P etri, Nuria A lburquerque, and Lorenzo B urgos
11 B lueberry (Vaccinium corymbosum L.). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Guo-Qing Song
ix