Table Of ContentAdvances in
Veterinary Dermatology
Advances in
Veterinary Dermatology
Volume 7
ProceedingsoftheSeventhWorldCongressofVeterinaryDermatology
Vancouver,Canada
July24–28,2012
Editedby
Sheila M. F. Torres
CollegeofVeterinaryMedicine,UniversityofMinnesota,StPaul,Minnesota,USA
Co-editors
Linda A. Frank
CollegeofVeterinaryMedicine,UniversityofTennessee,Knoxville,Tennessee,USA
Ann M. Hargis
DermatoDiagnostics,Edmonds,Washington,andDepartmentofComparativeMedicine,SchoolofMedicine,UniversityofWashington,Seattle,
Washington,USA
Thiseditionfirstpublished2013
(cid:2)2013TheAuthors.Journalcompilation(cid:2)2013ESVDandACVD
BlackwellPublishingwasacquiredbyJohnWiley&SonsinFebruary2007.Blackwell’spublishingprogrammehasbeen
mergedwithWiley’sglobalScientific,Technical,andMedicalbusinesstoformWiley-Blackwell.
Registeredoffice
JohnWiley&SonsLtd,TheAtrium,SouthernGate,Chichester,WestSussex,PO198SQ,UnitedKingdom
Editorialoffices
9600GarsingtonRoad,Oxford,OX42DQ,UnitedKingdom
2121StateAvenue,Ames,Iowa50014-8300,USA
Fordetailsofourglobaleditorialoffices,forcustomerservicesandforinformationabouthowtoapplyforpermissionto
reusethecopyrightmaterialinthisbookpleaseseeourwebsiteathttp://www.wiley.com/wiley-blackwell.
TherightoftheauthorstobeidentifiedastheauthorsofthisworkhasbeenassertedinaccordancewiththeUKCopy-
right,DesignsandPatentsAct1988.
Allrightsreserved.Nopartofthispublicationmaybereproduced,storedinaretrievalsystem,ortransmitted,inanyform
orbyanymeans,electronic,mechanical,photocopying,recordingorotherwise,exceptaspermittedbytheUKCopyright,
DesignsandPatentsAct1988,withoutthepriorpermissionofthepublisher.
Designationsusedbycompaniestodistinguishtheirproductsareoftenclaimedastrademarks.Allbrandnamesandpro-
duct names used in this book are trade names, service marks, trademarks or registered trademarks of their respective
owners.Thepublisherisnotassociatedwithanyproductorvendormentionedinthisbook.Thispublicationisdesignedto
provideaccurateandauthoritativeinformationinregardtothesubjectmattercovered.Itissoldontheunderstandingthat
the publisher is not engaged in rendering professional services. If professional advice or other expert assistance is
required,theservicesofacompetentprofessionalshouldbesought.
LimitofLiability⁄DisclaimerofWarranty:Whilethepublisherandauthorhaveusedtheirbesteffortsinpreparingthisbook,
theymakenorepresentationsorwarrantieswiththerespecttotheaccuracyorcompletenessofthecontentsofthisbook
and specifically disclaim any implied warranties of merchantability or fitness for a particular purpose. It is sold on the
understandingthatthepublisherisnotengagedinrenderingprofessionalservicesandneitherthepublishernortheauthor
shallbeliablefordamagesarisingherefrom.Ifprofessionaladviceorotherexpertassistanceisrequired,theservicesofa
competentprofessionalshouldbesought.
ISBN:9781118644874
ISSN:0959-4493(Print)
ISSN:1365-3164(Online)
AcataloguerecordforthisbookisavailablefromtheBritishLibrary.
Wileyalsopublishesitsbooksinavarietyofelectronicformats.Somecontentthatappearsinprintmaynotbeavailablein
electronicbooks.
CoverImagecredits:CanadaPlaceSails(courtesyofCanadaPlaceandPortMetroVancouver);Networkpathwaysgener-
ated through the use of IPA (Ingenuity(cid:3) Systems, www.ingenuity.com); Mycobacterial granuloma (Mycobacterium sp.
strain Tarwin) on the nasal planum and adjacent skin of a cat from rural Victoria, Australia. (Photograph courtesy of
DrRobertHilton,Yarrambat,Australia.)
Coverdesignby AndyMeaden
SetinUnivers-Light9/11.5pointsbySPS
PrintedinSingapore
Contents
Sponsors viii
OfficersandOrganizingCommittees ix
MembersoftheAdministrativeCommitteeoftheWAVD x
Preface xi
PART1ALLERGY
1.1 Epidemiologyofhumanatopicdermatitis–sevenareasofnotableprogressandsevenareasofnotableignorance 3
HywelC.Williams
1.2 Thegenomicsrevolution:willcanineatopicdermatitisbepredictableandpreventable? 10
TimNuttall
1.3 Serumanti-StaphylococcuspseudintermediusIgEandIgGantibodiesindogswithatopicdermatitisandnonatopicdogs 19
JenniferBexley,TimothyJ.Nuttall,BruceHammerberg,J.RossFitzgeraldandRichardE.Halliwell
1.4 Characterizationofcaninefilaggrin:genestructureandproteinexpressionindogskin 25
SatokoKanda,TakashiSasaki,AikoShiohama,KojiNishifuji,MasayukiAmagai,ToshirohIwasakiandJunKudoh
PART2IMMUNOLOGY
2.1 Innateimmunedefensesystemoftheskin 35
MaryamAfsharandRichardL.Gallo
2.2 Evaluationofcanineantimicrobialpeptidesininfectedandnoninfectedchronicatopicskin 42
DomenicoSantoro,DavidBunick,ThomasK.GravesandMariangelaSegre
2.3 Interleukin-31:itsroleincaninepruritusandnaturallyoccurringcanineatopicdermatitis 51
AndreaJ.Gonzales,WilliamR.Humphrey,JamesE.Messamore,TimothyJ.Fleck,GregoryJ.Fici,JohnA.Shelly,JanetF.
Teel,GaryF.Bammert,StevenA.Dunham,TroyE.FullerandRobertB.McCall
2.4 Expressionofthymicstromallymphopoietinincanineatopicdermatitis 57
JolantaKlukowska-Ro¨tzler,LudovicChervet,ElianeJ.Mu¨ller,PetraRoosje,ElianeMartiandJozefJanda
PART3SKINBIOLOGY
3.1 Thestratumcorneum:therampartofthemammalianbody 65
KojiNishifujiandJiSeonYoon
3.2 Fixingtheskinbarrier:past,presentandfuture–mananddogcompared 78
RosannaMarsella
3.3 Autosomalrecessiveichthyosisingoldenretrieverdogs:distributionandfrequencyofthePNPLA1mutantalleleindifferent
populations* 82
EricGuaguere,AnneThomas,AnaisGrall,EmmanuelleBourrat,LaetitiaLagoutte,FrederiqueDegorce-Rubiales,Christophe
Hitte,EmmanuelBensignor,JacquesFontaine,DidierPin,GuillaumeQueneyandCatherineAndre
3.4 Epidermalstructurecreatedbycaninehairfolliclekeratinocytesenrichedwithbulgecellsinathree-dimensionalskinequiva-
lentmodelinvitro:implicationsforregenerativetherapyofcanineepidermis 85
TetsuroKobayashi,KaoruEnomoto,YuHsuanWang,JiSeonYoon,RyokoOkamura,KaoriIde,ManabuOhyama,Toshio
Nishiyama,ToshirohIwasakiandKojiNishifuji
*ThisstudywasnotpublishedinthespecialissueoftheVeterinaryDermatologyjournal(VDE2013;24:1).
vi Contents
3.5 Skinlipidprofilinginnormalandseborrhoeicshihtzudogs 92
Ji-SeonYoon,KojiNishifuji,SinpeiIshioroshi,KaoriIdeandToshirohIwasaki
PART4THERAPY
4.1 Stemcelltherapyinveterinarydermatology 101
RobertJ.Harman
4.2 Asystematicreviewofrandomizedcontrolledtrialsforpreventionortreatmentofatopicdermatitisindogs:2008–2011
update 108
ThierryOlivryandPetraBizikova
4.3 Theeffectofketoconazoleonwholebloodandskinciclosporinconcentrationsindogs 129
LauraL.Gray,AndrewHillier,LynetteK.ColeandPa¨iviJ.Rajala-Schultz
4.4 InvitroantisepticsusceptibilitiesforStaphylococcuspseudintermediusisolatedfromcaninesuperficialpyodermainJapan 137
NobuoMurayama,MasahikoNagata,YuriTerada,MioOkuaki,NoriyukiTakemura,HidemasaNakaminamiandNorihisa
Noguchi
4.5 Photodynamictherapyforpythiosis 141
LaylaPires,SandradeM.G.Bosco,NelsonF.daSilvaJuniorandCristinaKurachi
PART5INFECTIOUSDISEASES
5.1 Thecanineandfelineskinmicrobiomeinhealthanddisease 151
J.ScottWeese
5.2 Ulceratedandnonulceratednontuberculouscutaneousmycobacterialgranulomasincatsanddogs 160
RichardMalik,BronwynSmits,GeorgeReppas,CarolineLaprie,CarolynO’BrienandJanetFyfe
5.3 Prevalenceofandriskfactorsforisolationofmeticillin-resistantStaphylococcusspp.fromdogswithpyodermainnorthern
California,USA 168
NicoleG.Eckholm,CatherineA.Outerbridge,StephenD.WhiteandJaneE.Sykes
5.4 Usefulnessofcefovecindisk-diffusiontestforpredictingmecAgene-containingstrainsofStaphylococcuspseudinterme-
diusandclinicalefficacyofcefovecinindogswithsuperficialpyoderma 176
KeitaIyori,YoichiToyoda,KaoriIde,ToshirohIwasakiandKojiNishifuji
5.5 SmallDemodexpopulationscolonizemostpartsoftheskinofhealthydogs 182
Iva´nRavera,LauraAltet,OlgaFrancino,ArmandSa´nchez,WendyRolda´n,SergioVillanueva,MarBardag´ıandLlu´ısFerrer
PART6ONCOLOGY
6.1 Advancesinthemanagementofskincancer 189
PamelaD.MartinandDavidJ.Argyle
6.2 Kinasedysfunctionandkinaseinhibitors 197
CherylA.London
6.3 Thecontributionofstemcellstoepidermalandhairfollicletumoursinthedog 204
ChiaraBrachelente,IlariaPorcellato,MonicaSforna,ElvioLepri,LucaMechelliandLauraBongiovanni
6.4 Epithelial-to-mesenchymal transition: immunohistochemical investigation of related molecules in canine cutaneous
epithelialtumours 211
LauraBongiovanni,AlessandraD’Andrea,MariaritaRomanucci,DanielaMalatesta,MelissaCandolini,LeonardoD.Salda,
LucaMechelli,MonicaSfornaandChiaraBrachelente
6.5 CanineinflamednonepitheliotropiccutaneousT-celllymphoma:adiagnosticconundrum 220
PeterF.Moore,VerenaK.AffolterandStefanM.Keller
PART7EQUINEDERMATOLOGY
7.1 Comparisonofhairfolliclehistologybetweenhorseswithpituitaryparsintermediadysfunctionandexcessivehairgrowth
andnormalagedhorses 231
MarieInnera˚,AnnetteD.Petersen,DanielleR.Desjardins,BarbaraA.Steficek,EdmundJ.RosserJrandHaroldC.SchottII
7.2 Equinesarcoidosis:clinicalsigns,diagnosis,treatmentandoutcomeof22cases 237
MarianneM.SloetvanOldruitenborgh-OosterbaanandGuyC.M.Grinwis
PART8WORKSHOPREPORTS
Co-editors:
DrKarenL.Campbell,UniversityofIllinois,Illinois,USA
DrKingaGortel,LakeCountryVeterinarySpecialistHospital,LakeCountry,BC,Canada
Contents vii
8.1 Nonpruritichairloss 247
R.CerundoloandJ.R.Rest
8.2 Dietarymanagementofskindisease:eliminationdietsanddietaryapproachtocanineallergicdisease 251
D.N.CarlottiandR.G.Harvey
8.3 Funwithlasers 257
M.BoordandC.S.Nett-Mettler
8.4 Allergen-specificimmunotherapy 264
A.HillierandJ.S.Pendergraft
8.5 Pododermatitis:canineinterdigitalfollicularcystsandfelineplasmacellpododermatitis 273
R.MuseandB.E.Wildermuth
8.6 Hottopicsinzoonosis 277
J.S.WeeseandC.C.Pye
8.7 Responsibleuseofantimicrobials 285
D.H.LloydandJ.D.Littlewood
8.8 Refractoryatopicdermatitistherapy 291
W.S.RosenkrantzandC.L.Mendelsohn
8.9 Challengesinotitis 298
A.Burrows,S.HobiandR.Albert
8.10 Allergytestingrevisited 305
R.E.W.HalliwellandS.Gilbert
8.11 Epidermalbarrierfunction 313
K.NishifujiandP.Bizikova
8.12 Thechangingfacesofparasitecontrol 319
C.TaylorandK.Glos
8.13 Topicalantimicrobialtherapy 323
K.BergvallandK.Varjonen
Index 331
Sponsors of the Seventh World Congress
of Veterinary Dermatology
PrincipalSponsors
MajorSponsors
DechraVeterinaryProducts
Elanco
SogevalSharedSolutions
SupportingSponsors Sponsors
Heska LDCA⁄Dermoscent
MerckAnimalHealth NestlePurinaPetCare
P&GPetCare⁄Iams RayneClinicalNutrition
VeterinaryAllergyReferenceLaboratory(VARL) Vetoquinol
Officers and Organizing Committees of
the Seventh World Congress of Veterinary
Dermatology
Officers PublicationsCommittee
President:DrManonParadis DrSheilaTorres(Chairperson)
Secretary:DrStephenWhite DrLindaFrank
Treasurer:DrRosarioCerundolo DrAnnHargis
ExecutiveOrganizingCommittee PublicityCommittee
TheOfficersand: DrRustyMuse(Chairperson)
DrDouglasDeBoer,Fundraising DrMandyBurrows(Co-chairperson)
DrAlanHoey,LocalOrganizingCommittee DrKingaGortel
DrChiaraNoli,Programme DrIanSpiegel
DrRustyMuse,Publicity DrClaudiaNett
DrSheilaTorres,Publications DrMasahikoNagata
Prof.RichardHalliwell,WAVDRepresentative
DrMasahikoNagata,AsiaRepresentative WAVDRepresentatives
Prof.RichardHalliwell(UK)
Committees
DrMasahikoNagata(Asia)
FundraisingCommittee
DrDouglasDeBoer(Chairperson)
DrWayneRosenkrantz(Co-chairperson)
LocalOrganizingCommittee
DrAlanHoey(Chairperson)
DrVincentDefalque(Co-chairperson)
ProgrammeCommittee
DrChiaraNoli(Chairperson)
SonyaBettenay(Co-chairperson)
MonaBoord(Co-chairperson)
KerstinBergvall
ValerieFadok
AndyHillier
KojiNishifuji
DavidRobson
ManolisSaridomichelakis
AnthonyYu
Members of the Administrative
Committee of the WAVD
KennethKwochka(USA) PresidentWAVD,RepresentativeofACVD
DidierNoelCarlotti(France) PastPresidentWAVD,PresidentWCVD8
AmandaBurrows(Australia) SecretaryWAVD,RepresentativeDCACVS
DavidLloyd(UK) TreasurerWAVD,PresidentWCVD5
PhilRoudebush(USA) RepresentativeofAAVD
LucBeco(Belgium) RepresentativeofESVD
EmmanuelBensignor(France) RepresentativeofECVD
ManonParadis(Canada) RepresentativeofCAVD,PresidentWCVD7
CarmelTaylor(HongKong) RepresentativeofAiSVD
ClaudiaNett-Mettler(Switzerland) Member-at-Large
WayneRosenkrantz(USA) Member-at-Large
RichardHalliwell(UK) PresidentWCVD1,PastPresidentWAVD
HansKoch(Germany) PresidentWCVD3
PeterIhrke(US) PresidentWCVD4
Preface
TheSeventhWorldCongressofVeterinaryDermatology,heldinVan- globe reflect, unquestionably, the worldwide advancement of the
couver,Canada,fromJuly24to28,wasunquestionablyasuccess! VeterinaryDermatologyfieldand,forthis,weshouldallbeproud.
TheCongresswasorganizedwiththesupportoftheWorldAssocia- This hardbound volume, Advances in Veterinary Dermatology 7,
tionforVeterinaryDermatology(WAVD)anditsaffiliatedsocieties.A containsthemanuscriptspublishedinthespecialissueinadditionto
recordnumberofmorethan1600delegatesfromover50countries theworkshopreports.Thememoryoftheotherscientificpresenta-
participated,reflectingtheworldwideinterestintheVeterinaryDer- tions ispreservedintheabstract issue ofVeterinaryDermatology,
matologyspecialty. Volume23Supplement1,July2012,andintheProceedingsofthe
The scientific programme was exceptional and covered a broad ContinuingEducationProgramme.
rangeoftopics.Speakersofsixstateoftheart,sixsupportingreview ThesuccessofthisCongressislargelyaresultofthehardworkof
and20supporting originalstudiespresentedcutting edgeinforma- theofficersandmembersofthevariousorganizingcommitteeswho
tionintheareasofallergy,immunology,skinbiology,therapy,infec- unpretentiouslydonatecountlesshoursoftheirtimetomakethisa
tiousdiseasesandoncology.The69freecommunicationsand121 memorableevent.Moreover,withoutthekindsupportofthecorpo-
postersprovideddiversehigh-qualityresearchandreportsofclinical ratesponsorsthisCongresswouldnotbepossible.Onbehalfofall
casescateringtothebroadinterestoftheaudience.Moreover,there participants,Iwouldliketotakethisopportunitytothankallthespon-
was an extensive and well-attended advance and comprehensive sorsoftheSeventhWorldCongressofVeterinaryDermatology.
continuingeducationprogrammeforveterinarypractitioners,notto IhopeyouarealreadymakingplansfortheEighthWorldCongress
mentiontheprogrammes exclusivelyfocusedinequine, felineand ofVeterinaryDermatologywhichwilltakeplaceinBordeaux,France,
exoticdermatology.Lastbutnotleast,14workshops,whereexperts andpromisestobeasoutstandingasthisone.
presentedtopicsinvariousareas,providedawonderfulopportunity
SheilaTorres
for colleagues to ask questions and exchange ideas in an informal
College of Veterinary Medicine, University of Minnesota, St Paul,
atmosphere. This large number of scientific presentations and the
Minnesota,USA
highcalibreofthematerialdeliveredbycolleaguesfromacrossthe
