Table Of ContentAdvances in
Mucosal
Immunology
Advances in
Mucosal
Immunology
Proceedings of the Fifth
International Congress of
Mucosal Immunology
Edited by
Thomas T MacDonald PhD MRCPath
Wellcome Senior Lecturer
Department of Paediatric Gastroenterology
St Bartholomews Hospital, London
Stephen J Challacombe PhD BDS MRCPath
Professor, Department of Oral Medicine
and Pathology
Guy's Hospital, London
Paul W Bland PhD
Lecturer, Department of Veterinary Medicine
The University, Bristol
Christopher R Stokes PhD
Senior Research Fellow, Department
of Veterinary Medicine
The University, Bristol
Richard V Heatley MD MRCP
Senior Lecturer, Department of Medicine
St James's University Hospital, London
Allan Mcl Mowat PhD MB ChB
Lecturer, Department of Bacteriology
and Immunology
The University, Glasgow
" KLUWER ACADEMIC PUBLISHERS
DORDRECHT/BOSTON/LONDON
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for all other countries: Kluwer Academic Publishers Group, Distribution Center, PO
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British Library Cataloguing in Publication Data
International Congress on Mucosal Immunology, 5th.
Advances in mucosal immunology
1. Man. Mucous membranes. Diseases. Immunological aspects
I. Title II. MacDonald, T.
616.3079
ISBN-13: 978-94-010-7323-3 e-ISBN-13: 978-94-009-1848-1
001: 10.1007/978-94-009-1848-1
Copyright
© 1990 by Kluwer Academic Publishers
Softcover of the hardcover 15t edition 1990
All rights reserved. No part of this publication may be reproduced, stored in a
retrieval system, or transmitted in any form or by any means, electronic,
mechanical, photocopying, recording or otherwise, without prior permission from
the publishers, Kluwer Academic Publishers BV, PO Box 17, 3300 AA Dordrecht,
The Netherlands.
Published in the United Kingdom by Kluwer Academic Publishers,
PO Box 55, Lancaster, UK.
Kluwer Academic Publishers BV incorporates the publishing programmes of
D. Reidel, Martinus Nijhoff, Dr W. Junk and MTP Press.
Contents
Preface xxxv
1 Local immunity: the human mucosa in health and disease
P Brandtzaeg, K Bjerke, TS Halstensen, M Hvatum, K Kett, P Krajci,
D Kvale, F Muller, D Wilsson, TO Roynum, H Scott, LM Sollid, P
Thrane and K Valnes
PART 1 BASIC MECHANISMS IN MUCOSAL IMMUNOLOGY
SECTION A: ANTIGEN HANDLING AND PROCESSING AT MUCOSAL
SURFACES
2 Intestinal processing of soluble protein antigen
PW Bland and CV Whiting 17
3 Antigen handling in the intestine mediated by normal enterocytes
L Mayer, E Siden, S Becker and D Eisenhardt 23
4 Ultrastructural distribution of Class II MHC molecules in mucosal
antigen presenting cells and their relationship to antigen uptake
and processing
G Mayrhofer, LDJ Spargo, GP Davidson and Z-H Huang 29
5 Mature la + murine intestinal epithelial cells with APC activity share
common antigens with gut interdigitating dendritic cells
D Kaiserlian, K Vidal and JP Revillard 34
6 Murine gut epithelial cells present antigen to specific T cell
hybridoma
D Kaiserlian, K Vidal, M Blanc and JP Revillard 38
7 The expression of Class II major histocompatability antigens in the
murine small intestine is not influenced by the oral administration of
cholera toxin
AD Wilson and CR Stokes 40
v
ADVANCES IN MUCOSAL IMMUNOLOGY
8 Suppression of Iymphoproliferation and interleukin-1 production by
enterocyte-derived factors
GT Pang, RL Clancy and HA Saunders 42
9 Human colon epithelial celllysates evoke monocyte chemotaxis
W Beeken, J Fabian, D Meyer and D Gump 44
10 Antigen handling at respiratory epithelial surfaces in the rat: a highly
developed network of dendritic APC revealed by a novel tissue
sectioning procedure
PG Holt, MA Schon-Hegrad and PG McMenamin 46
11 Induction of Class II MHC antigens in the rat digestive system after
systemic application of recombinant gamma interferon
B Steiniger, P Falk, M Lohmill/er and PH van der Meide 48
12 Aat intestinal lamina propria macrophages: characterisation and
accesory role in ovalbumin-induced T cell proliferation
DM Kambarage and PW Bland 50
13 The effect of interferon gamma (IFN-y) on MHC Class II (la) antigen
by expression on the neonatal enterocyte
A Hughes, KJ Bloch, D Gillen, A Bhan, V Giovino and PR Harmatz 52
SECTION B: INTRAEPITHELIAL LYMPHOCYTES AND
LYMPHOEPITHELIAL INTERACTIONS
14 Subsets of gamma/delta T cells in human intestinal epithelium
J Spencer, PG Isaacson, TC Diss and IT MacDonald 57
15 Increased numbers of T cell receptor gamma/delta bearing
lymphocytes in the epithelium of coeliac patients
£ Savilahti, A Arato and M Verkasalo 61
16 The gamma/delta T cell receptor (TCA) is expressed on less than
50% of intraepitheliallymphocytes (lEL) in human intestine
R UI/rich, H Schieferdecker, C Brunn, £0 Riecken and M Zeitz 67
17 Expression of T cell receptors TcA1 (gamma/delta) and TcA2
(alpha/beta) in the human intestinal mucosa
LK Trejdosiewicz, CJ Smart, DJ Oakes, A Calabrese, PD Howdle
and AW Boylston 69
18 Analysis of intestinal intraepitheliallymphocytes in athymic (nude)
and SCID mice
B de Geus, LD Shulz, M van der £nden, J Coolen and J Rozing 71
vi
CONTENTS
19 Alpha/beta T cell receptor expression in the intestinal epithelium of
rodents
JL Viney, PJ Kilshaw and IT MacDonald 77
20 Do all murine intraepithelialleukocytes (IEL) have T cell receptor
rearrangements?
K Croitoru, J Bienenstock, R Stead, I Ishida, K Ito, S Tonegawa and
PB Ernst 81
21 T cell receptor expression by CD8 + intraepithelial lymphocytes
from mouse small intestine
A Mcl Mowat, AJ Edwards and IN Crispe 83
22 Tissue distribution and characteristics of an activated T cell
sub-population from rabbit gut associated lymphoid tissues
A Ruiz and J Tseng 86
23 HML-1, an antibody raised against intestinal lymphocytes,
recognises an antigen appearing on activated peripheral blood
lymphocytes
HL Schieferdecker, R Ullrich, AN Weiss-Breckwoldt, R Schwarting,
H Stein, EO Riecken and M Zeitz 89
24 Lymphoepithelial interactions associated with proximal colonic
lymphoid tissue in the mouse
SK Saxena, GA Perry, SS Joshi and JG Sharp 91
25 Salmonella increases M cell numbers in mouse Peyer's patch
follicle associated epithelium
TC Savidge, MW Smith, PS James and P Aldrid 95
26 Immunohistological studies of lymphocyte subsets in normal cervix,
HPV infection and cervical intraepithelial neoplasia
JG McKenzie, A King, MJ Hare, T Fulford and M Stanley 99
27 Phenotype of intraepitheliallymphocytes in oral lichen planus
PM Farthing and S Cannon 101
28 Thermolysin treatment; an improved dispersion technique for
isolating functional lymphoid cells from intestinal tissues
I Nordstrom, M Quiding, B Kjelsson, A Kilander, E Ahlfors, J
Holmgren and C Czerkinsky 103
29 A novel carbohydrate epitope on the surface of mouse
intraepitheliallymphocytes
PJ Kilshaw 105
vii
ADVANCES IN MUCOSAL IMMUNOLOGY
SECTION C: CYTOKINES IN RELATION TO MUCOSAL IMMUNITY
30 Cytokine/lymphokine regulation of IgA B cell differentiation
GR Harriman and W Strober 109
31 Role for interleukins 5 and 6 in IgA synthesis
KW Beagley, JH Eldridge, W Aicher, T Hirano, T Kishimoto, FLee, H
Kiyono and JR McGhee 114
32 Regulaton of intestinal immunity by soluble Band T cell products
WK Aicher, KW Beagley, MG Bruce, H Kiyono and JR McGhee 118
33 Th1 and Th2 cells in mucosal associated tissues
T Taguchi, JR McGhee, RL Coffman, KW Beagley, JH Eldridge, K
Takatsu and H Kiyono 120
34 Recombinant murine IL-5 enhances IgA production by rat splenic B
cells
DW McGhee, KW Beagley and JR McGhee 124
35 Human interleukin-5 "switches" human IgA + B cells to express and
secrete IgA
IN Bertolini and EM Benson 126
36 Regulation of human IgA and IgA subclass synthesis by
recombinant interleukin-6
K Fujihashi, CLue, KW Beagley, Z Moldoveanu, T Hirano, T
Kishimoto, J Mestecky, JR McGhee and H Kiyono 129
37 Recombinant interleukin-6 enhances specific antibody secretion by
in vivo activated human B cells
CLue, JR McGhee, H Kiyono, Z Moldoveanu, K Fujihashi, T
Kishimoto, T Hirano and J Mestecky 131
38 The role of T-cell Iymphokines in congenital immunoglobulin
deficiency
ME Brodtmann and EM Benson 134
39 A clonal microculture that supports IgA expression by murine B cells
A George, RL Kerlin, CE Schrader and JJ Cebra 136
40 Regulation of B cell differentiation by T cells obtained from mucosal
and systemic lymphoid tissues
SL Tonkonogy 140
41 Spontaneous gamma-interferon production by human tonsillar
lymphocytes: regulation of secretion by interleukin-1
M Quiding, I Nordstrom, G Granstrom, J Holmgren and C
Czerkinsky 142
viii
CONTENTS
42 Interleukin-6 is secreted by epithelial cells in response to
Gram-negative bacterial challenge
S Hedges, P de Man, H Linder, CV Kooten and C Svanborg Edfm 144
43 IL-6 production by rat mast cell lines?
JS Marshall, J Bienenstock and J Gauldie 148
SECTION 0: MUCOSAL T CELL RESPONSES and IMMUNO REGULATION
44 T cell activation and inhibition by cholera toxin and its B subunit
CO Elson, S Solomon and S Woogen 153
45 The role of cytophilic IgA and Fea receptors in circulating T cells in
autoimmune diseases
T Lehner and F Fortune 158
46 Analysis of IgA binding to FeaR + T cells and characterization of
anti-FeaR antiserum
WK Aicher, H Kiyono, ML McGhee, KW Beagley, T Taguchi, J Xu,
TF Meyer and JR McGhee 163
47 Functional potential of gut T and B cell subsets from mucosally
primed mice
JJ Cebra, CK Cebra, CF Cuff, A George, RL Kerlin, AC Logan, DH
Rubin, CE Schrader and PD Weinstein 167
48 Role of intestinal T cells in pulmonary immunity to non-typable
Haemophilus influenza
AW Cripps, FJ Wallace, RL Clancy and AJ Husband 172
49 Mucosal immunoregulation by CD4+ (L3T4+) T cells
MG Bruce, T Taguchi, KW Beagley, WA icher, H Kiyono and JR
McGhee 176
50 T-Cell subset distributions in spleen, thymus and mesenteric lymph
nodes from immunodeficient wasted mice
GE Woloschak and CR Libertin 180
51 Postnatal appearance of lymphocytes in the lamina propria of the
gut mucosa of pigs: number, proliferation and T lymphocyte
subsets
HJ Rothkotter, H Ulbrich and R Pabst 182
52 T cells associated with transfer of mucosal immunity to Trichinella
spiralis
CM Mink, ALW van Vliet, WE Bernadina and EJ RUitenberg 184
ix
ADVANCES IN MUCOSAL IMMUNOLOGY
53 Rat T cell subpopulations: Effect of environmental and microbial
antigens
J Katz, JH Eldridge and SM Michalek 186
54 Mucosal injury in athymic nude rats following infection with
Nippostronglylus brasiliensis
MH Perdue, PE Ernst and R D'lnca 188
55 Cytotoxic T cells in gut associated lymphoid tissue of the rat
B Young, P Chee, ML Dunkley and AJ Husband 190
56 Small intestinal glycoprotein synthesis and secretion: effects of T
lymphocyte activation
JE Crabtree, RV Heatley and MS Losowsky 192
57 Phenotypes and functions of lamina propria lymphocytes from
swine gut
H Salmon, C Paulus, M Olivier, P Berthon, I Lantier, S Bernard and
E Bottreau 195
SECTION E: CELL MIGRATION IN THE MUCOSAL IMMUNE SYSTEM
58 Effector cell migration in mucosal defence
AJ Husband, ML Dunkley and VL Clifton 199
59 Search for B cells ready to emigrate from sheep ileal Peyer's patch
JD Reynolds, B Motyka, S Brook and P Griebel 205
60 Role of antigen in migration patterns of T cell subsets arising from
gut associated lymphoid tissue
ML Dunkley and AJ Husband 209
61 Emigration of lymphocytes from the ileal Peyer's patch to the
intestinal mucosa in sheep
EJ Gyorffy and JD Reynolds 213
62 Computer assisted analysis of lymphocyte migration
CO Ottaway and LL Fisher 217
63 Characterisation of the lymphocyte-glandular mucosal tissue
interaction using an in vitro adherence assay
NL O'Sullivan and PC Montgomery 222
64 In vivo adherence of lymphocytes to high endothelial venules of
Peyer's patches and peripheral lymph nodes
Jeenan Tseng, Than Aw and A Rosado 227
x
CONTENTS
65 In vivo migration and DTH responses by murine antigen-specific T
cell lines
CJ Smart, PD Howdle and LK Trejdosiewicz 230
66 Direct transdiaphragmatic traffic of peritoneal macrophages to
lung-associated lymphatic tissue
MLM Pitt and AD Anderson 232
SECTION F: MODULATION OF MUCOSAL RESPONSES
67 Sympathetic neuromodulation of systemic anaphylaxis
L Carter, K Ramaswamy, R Mathison, D Kirk, JS Davison and D
Befus 239
68 The induction of specific oral tolerance can be abrogated by
exposure to certain unrelated antigens
SJ Challacombe, A Armitage, J Gall and M Biggerstaff 243
69 The translocation of immunoglobulin A and of albumin in the rat
intestine is mediated by prostaglandins
S Freier and M Eran 246
70 Androgen regulation of the ocular secretory immune system
DA Sullivan and LE Hann 250
71 Modulation of the in vivo anti-rotavirus humoral immune response
by neuroendocrines and neuropeptides
MK Ijaz, D Dent and LA Babiuk 255
72 Altered responsiveness to fed or injected protein in piglets given
antigen at birth
M Bailey, B Miller, E Telemo and CR Stokes 262
73 Effect of aging on induction of oral tolerance by intragastric
administration of sheep red blood cells in mice
T Hosokawa, A Aioke, K Nakamura, K Koyama, A Yoshida, N Marui,
Y Nishi and K Kawai 264
74 The induction of oral tolerance in germ free and conventional
Balb/c mice
A Armitage and SJ Challacombe 266
75 Prevention of collagen-induced arthritis by oral administration of
encapsulated Type II collagen
HSG Thompson, N Harper, T Assadullahi, RC Hider and NA Staines 268
xi
