Table Of ContentAdvances in High Pressure Bioscience
and Biotechnology
Horst Ludwig (Editor)
Advances
in High Pressure Bioscience
and Biotechnology
Proceedings of the International Conference
on High Pressure Bioscience and Biotechnology,
Heidelberg, August 30 - September 3, 1998
, Springer
Editor
Professor Dr. Horst Ludwig
University of Heidelberg
Institute for Pharmaceutical Technology
and Biopharmacy
- Section Physical Chemistry -
1m Neuenheimer Feld 346
D - 69120 Heidelberg, Germany
e-mail:
Preface
The International Conference on High Pressure Bioscience and Biotechnology at
Heidelberg continued the tradition of Japanese and European joint meetings,
following the conferences at La Grand Motte, near Montpellier (1992), Kyoto
(1995), and Leuven (1996). The first meeting at Montpellier, promoted by Claude
Balny (INSERM, Montpellier, France) and Rikimaru Hayashi (Kyoto University,
Japan) stimulated many scientists and practitioners, especially from Europe and
Japan. At Heidelberg (1999), it was a pleasure to welcome 153 participants from
20 countries, among them large delegations from Japan and the USA.
This book records the contributions presented at the Heidelberg meeting and
shows the great potential of high pressure technology. It covers the whole range of
current high pressure bioscience, from marine microbiology and investigations on
deep-sea diving to applications in food science. High pressure can be used to
process foods and sterilize pharmaceutical products; moreover it is a valuable tool
for fundamental research. For example, pressurized proteins exhibit intermediate
states of their folding and unfolding pathways.
We hope that this publication will stimulate further developments in basic and
applied research as well as foster fruitful contacts between industry and academia.
Heidelberg, June 1999 Horst Ludwig
Scientific Committee
K. Autio, Espoo; C. Barny, Montpellier; D. H. Bartlett, San Diego; 1.e. Cheftel,
Montpellier; P. Colman, Ternse; 1. Frank, Delft; R. Hayashi, Kyoto; M. Hendrickx,
Leuven; K. Heremans, Leuven; C. Kato, Yokosuka; D. Knorr, Berlin; S.Kunugi,
Kyoto; A. Macdonald, Aberdeen; P. Masson, La Tronche; E. Raetz, Lausanne; J.
Smelt, Vlaardingen; Y. Tanigushi, Kyoto; P. Taoukis, Athens; B. Tauscher,
Karlsruhe; 1. Westerlund, Vaesteraas; R. Winter, Dortmund.
Local Organizing Committee
H. Ludwig (Chairman), K.-H. Loebel (Conference Secretary), R. Eicher, C.
Holters, I. Koser, N. Merkulow, M. Schauer, E. Schinko, Ch. Schreck, E.
Sternberger.
The conference has been supported by:
Deutsche Forschungsgemeinschaft, Bonn, Germany
and the following companies:
ABB Pressure Systems, Vasteras, Sweden; Bayer AG, Wuppertal, Germany;
ANTITOXIN Dr. Helmbold GmbH, Meckesheim, Germany; Engineered Pressure
Systems International N.V., Temse, Belgium; GEC ALSTHOM ACB, Nantes,
France; Goedecke AG, Freiburg, Germany; Heidelberger Zement AG, Heidelberg,
Germany; Hoechst Marion Roussel GmbH, Frankfurt am Main, Germany;
Mannesmann Rexroth GmbH, Lohr am Main, Germany; Dr. Eduard Ritsert GmbH,
Eberbach, Germany; Sitec Sieber Engeneering AG, ZUrich, Schweiz; Stansted
Fluid Power Ltd, Stansted, UK; Uhde Hochdrucktechnik, Hagen, Germany;
Unilever Research Laboratorium, Vlaardingen, Netherlands
Acknowledgements:
We would like to express our gratitude and special appreciation to Mr. Karl-Heinz
Loebel, conference secretary, for his self-sacrificing and successful work before
and during the conference and to Ms Claudia Holters and Mr. Ingmar Koser for
their assistance in preparing this conference proceedings.
Table of Contents
Microorganisms from the Deep-Sea
The Properties of Deep-Sea Piezophilic Bacteria and Their Possible Uses
in Biotechnology
A.A. Yayanos ............................................................................................................ 3
Changes in the Microbial Community in Deep-Sea Sediment
During Cultivation Without Decompression
C. Kato. M. Yanagibayashi. Y Nogi. L. Li and K. Horikoshi ................................ 11
Microbial Communities in the World's Deepest Ocean Bottom-
The Mariana Trench
L. Li. C. Kato and K. Horikoshi ............................................................................. 17
An Expression of a Cold Shock Inducible Gene CspA
Under Hydrostatic Pressure in Deep-Sea Barophilic Bacterium,
Shewanella sp. Strain DSS12
S. Fujii. K. Nakasone and K. Horikoshi ................................................................. 21
Microorganisms
Interaction Between Osmotic and Hydrostatic Pressure in Yeast Inactivation
J.M. Perrier-Cornet. M. Hayert. E. Saurat. C. Milesse and P. Gervais ................. 27
Multiple Stress Resistance in Pressure Resistant Escherichia coli Mutants
K. Hauben. T Nystrom. A. Farewell and C. Michiels ............................................ 31
Strain Differences in Resistance to High Pressure and Mild Heat in Isolates
of Escherichia coli 0157
A. Benito and B. Mackey ............................................................................ ............ 35
Hsc70 Is Not Essential for Thermotolerance but for Barotolerance
H. Iwahashi. S. Nwaka. K. Obuchi and Y Komatsu ............................................... 39
Ribosome Analysis In Vivo by Differential Scanning Calorimetry:
The Effects of High Pressure on Escherichia coli
G. W. Niven and B.M. Mackey ................................................................................ 43
Measurement of Microbial Activities Under High Pressure by Calorimetry
K. Tamura. Y Kamiki and M. Miyashita ................................................................ 47
Use of a Fatty Acid Auxotroph To Study the Role
of Membrane Fatty Acid Composition on the Pressure Resistance
of Escherichia coli
M.A. Casadei and B.M. Mackey ............................................................................. 51
VIII Table of Contents
High Pressure Inactivation of Heat Activated Ascospores
of the Mould Eurotium repens
R. Eicher and H. Ludwig ........................................................................................ 55
Microorganism Inactivation Using High Pressure Generation
in Sealed Vessels Under Sub-Zero Temperature
K. Hayakawa, Y. Ueno, S. Kawamura, T. Kado, K. Kamiyama, Y. Tamaoka.
M. Morishita, M. Matsumoto, S. Kawai and R. Hayashi ........................................ 59
High Pressure Inactivation of Anaerobic Spores
from Clostridium pasteurianum
C. Holters, G. van Almsick and H. Ludwig .... ........................................................ 65
Neutral Trehalases Contribute to Barotolerance in Saccharomyces cerevisiae
H. Iwahashi, S. Nwaka, K. Obuchi and Y. Komatsu ............................................... 69
Ultra High Pressure Treatment of Streptomyces sp. R61
W Kurzatkowski, J Solecka, B. Rozbicka, J Filipek. A. Laudy,
M. Fonberg-Broczek and S. Porowski .................................................................... 73
The Effects of Sub-Lethal Pressure Treatment on Escherichia coli 0157:H7
M. Linton, G.J. /vfCann and M.F. Patterson ......................................................... 77
Inactivation Kinetics of Fungal Conidiospores
N. ." 4erkulow and H. Ludwig .................................................................................. 81
Acquired Barotolerance and Thennal Stability of Cellular Components
at High Pressure in Saccharomyces cerevisiae Cells
K. Obuchi, H. Iwahashi and Y. Komatsu ....................................................... ......... 85
Role of Oxidative Stress in the Inactivation of Escherichia coli
by High Hydrostatic Pressure
M. Robey, s.F. Park and B.M. Mackey ........ .......................................................... 89
Effects of Heat and Pressure Shock on Growth Thennograms of Yeast
M. Miyashita and K. Tamura ................................................................................. 93
Description of Listeria Behaviour Under Various Pressurization Strategies
R.F. Vogel, V. Herdegen and P. Klostermaier ....................................................... 97
Microorganisms in Food
Pathogen Inactivation by High Pressure Treatment of Foods
M.F. Patterson, M. Linton and JM.J McClements ............................................. 105
Building Fail-Safe Models To Describe the Effect of Temperature
and Pressure on the Kinetics of Inactivation of Infectious Pathogens in Foods
J C. Hellemons and JP.P.M. Smelt.. ....... ............................................................. 109
Table of Contents IX
Advances in Bacterial Spores Inactivation in Thermal Treatments
Under Pressure
P. Rovere, N G. Lonnerborg, S. Gola, L. Miglioli, N Scaramuzza
and N Squarcina .................................................................................................. 113
Effect of Supercritical Carbon Dioxide on Some Bacteria and Yeasts
of Interest in the Food Industry
F. Dellaglio, V. Dallacasa, A. Venturi, A. Bertucco and P. Pal/ado ................... 121
The Effect of High Pressure on the Microbial Quality of Apple Juices
A. Boyd, M.F. Patterson, A. McCracken and B. Mackey ..................................... 125
Thermal Effect Before High Pressure Treatment on the Reduction
of Enterococcus faecalis in Duck Fatty Liver
P. Ballestra, C. Cruz, M. Antoine, A. Largeteau, G. Demazeau, D. Le Ba,
F. Zuber and A. EI Moueffak ................................................................................ 129
High Pressure Inactivation and Survival
of Pressure-Resistant Escherichia coli Mutants in Milk
C. Garcia-Graells, B. Masschalck, N Moonjai and C. Michiels ......................... 133
Membranes and Lipids
Some Preliminary Measurements of the Effect of High Hydrostatic Pressure
on the Porin Omp C from E. coli
A.G. Macdonald and B. Martinac ........................................................................ 139
New Transition ofDioleoylphosphatidylcholine Bilayer Membrane
Under High Pressure
S. Kaneshina, H /chimori, T. Hata and H Matsuki ............................................ 145
Effect of Pressure on the Phase Transitions
of Dimyristoylphosphatidylethanolamine
and Dimyristoylphosphatidylcholine Bilayers
H Jchimori, S. Endo, T. Hata, H Matsuki and S. Kaneshina .............................. 149
Solidification and Melting of Some Edible Fats and Model Lipid Systems
Under Pressure
W. Buchheim, E. Frede, M. WolfandP. Baldenegger ......................................... 153
The Effects of High Pressure on Signal Transducing Systems
of Cultural Endothelial Cells
L.B. Buravkova ..................................................................................................... 157
Thermotropic and Barotropic Phase Behavior
of I-Hexadecyl-2-Pa1mitoylphosphatidylcholine Bilayer Membrane
H. /chimori, H Matsuki, T. Hata and S. Kaneshina ............................................ 161
X Table of Contents
Effect of Pressure and Local Anesthetics on the Phase Transitions
of Ether-Linked Dihexadecylphosphatidylcholine Bilayer Membrane
S. Kaneshina, H. Endo, T. Hata and H. Matsuki .................................................. 165
Proteins
Pressure-Induced Thermostabilization of Glutamate Dehydrogenase
from the Hyperthermophiles Pyrococcus furiosus and Thermococcus litoralis
M.M. Sun, N. Tolliday, C. Vetriani, F. T. Robb and D.S. Clark ............................ 171
High-Resolution NMR Studies of Proteins Under High Pressure
J. Jonas ................................................................................................................ 177
Pressure-Temperature Induced Unfolding and Aggregation of Proteins
K. Heremans ......................................................................................................... 183
Comparison of the Pressure-Temperature Behavior of Neutral Proteases
from Thermophilic and Psychrophilic Bacteria
S. Kunugi, H. Ikeuchi and K. Oda ........................................................................ 187
Cold Denaturation of Proteins Under Pressure Studied
by Time-Resolved Fluorescence. Application to Ribonuclease P2
from S. sofataricus
P. Tauc, P. Fusi, P. Tortora, R. Lange and J.c. Brochon .................................... 191
High Pressure Fluorescence Studies of Porphyrins and Hemoproteins
L. Smeller, K. Kis-Petik, A.D. Kaposi and J. Fidy ............................................... 195
Pressure-Induced Rearrangement of Aryl-Iron Complexes
in Cytochrome P-450cam: Application to Topological Mapping
of the Compressed Active Site
G. Hui Bon Hoa, R.A. Tschirret-Guth and P.R. Ortiz de Montellano .................. 199
The Dynamics of Protein-Bound Water in the Heme Domain ofP450BM3
as Compared with P450cam and P450 2B4
D.R. Davydov, G. Hui Bon Hoa and J.A. Peterson .............................................. 203
Use of High Pressure To Investigate the Role of Carbohydrate Moiety
in the Conformational Stability of Carboxypeptidase Y
M. Dumoulin, H. Ueno, R. Hayashi and C. Balny ............................................... 207
Complex Unfolding Events of Ss07d from S. solfataricus
at High Temperature and High Pressure
R. Lange, E. Mombelli, J. Connelly, M. Afshar, P. Fusi, C. Balny
and P. Tortora ...................................................................................................... 211
Influence of Pressure on the Reactivity of Antigens and Antibodies.
Application to the Follow-up of Conformational Changes
P. Lemay, L. Estevez, S. Espei/lac and P. Degraeve ............................................ 215
Table of Contents XI
Cold, Heat and Pressure Denaturation of Horse Heart Metmyoglobin:
A FTIR Study
F. Meersman, L. Smeller and K. Heremans ......................................................... 219
Hydrogen-Deuteriwn Exchange Versus Conformational Changes in Proteins.
A Two-Dimensional FTIR Approach
L. Smeller and K. Heremans ................................................................................ 223
Pressure and Temperature Stability of ~-Galactosidases: A Structural
and Functional Study
P. Rubens, P. Degraeve, P. Lemay and K. Heremans .......................................... 227
Conformational Changes of Lipoxygenase at Elevated Pressure
and Temperature
P. Rubens, N. Bee, R. Lange, C. Balny, J. Frank and K. Heremans .................... 231
Hydrogen-Deuteriwn Exchange of Lipoxygenase at High Pressure
and Temperature
P. Rubens, J. Frank and K. Heremans ................................................................. 235
High Hydrostatic Pressure Effect on Activity
of Circulating Immune Complex Formation in Blood Serum
N. V Shishkova, L1 Donehenko, B1 Barbashova and E. V Ulyanova ................ 239
Pressure Effect on the Temperature Unfolding and Gelation of Myoglobin
L. Smeller, P. Rubens and K. Heremans .............................................................. 243
The Influence of High Pressure Treatment on the Spectroscopic Properties
of Ovalbumin
D.D.S. Smith, VB. Galazka and /.G. Sumner ................................................ ....... 247
Contribution of Hydrophobic Residues to the Stability
of Ribonuclease A Chain Folding Initiation Site Mutants. A Comparison
of Pressure and Temperature Induced Unfolding
J. Torrent, J.P. Connelly, M. G. Coli, M RiM, R. Lange and M Vilanova .......... 251
Pressure Effect on Hydration of Biomolecules
T. Ooi ................................................................................................................... 255
Enzymes
High Pressure Enzyme Kinetics
C. Balny ................................................. ............................................................... 261
The Single Cysteinyl Residue (Cys341) of Carboxypeptidase Y
for Cavity Control in the S 1 Substrate Binding Pocket
G. lung, H. Ueno and R. Hayashi ........................................................................ 267
Effects of Hydrostatic and Osmotic Pressures on Dealkylation (Aging)
of Phosphylcholinesterase Adducts
C. Clery, P. Guerra, A. Reds/ob and P. Masson .................................................. 271
