Table Of ContentACTA MEDICIN.tE
LEGALIS
Vol. XLIV
1994
P. Mangin B. Ludes (Eds.)
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P. Mangin B. Ludes
(Eds.)
ACTA MEDICINlE
LEGALIS
Vol. XLIV
1994
XVIth Congress of the International Academy
of Legal Medicine and Social Medicine
Strasbourg, France, 31 May - 2 June, 1994
XVIeme Congres de l' Academie Internationale
de Medecine Legale et de Medecine Sociale
Strasbourg, France, 31 mai-2 juin 1994
Springer
Professor Dr. Patrice Mangin
Universite de Strasbourg
Institut de Medecine Legale
11, rue Humann
F-67085 Strasbourg
Dr. Bertrand Ludes
Universite de Strasbourg
Institut de Medecine Legale
11, rue Humann
F-67085 Strasbourg
The authors would like to thank Ms Anne Tricot for her help
in the production of the book.
ISBN-13:978-3-540-58847-4 e-ISBN-13:978-3-642-79523-7
DOl: 10.1007/978-3-642-79523-7
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Preface
Professor Patrice MANGIN
President of the XVlth Congress of the International Academy
of Legal Medicine and Social Medicine
The International Academy of Legal Medicine and Social Medicine was founded in
1938 in Bonn. The motive for founding the Academy was to promote associating and
confronting on an international background the scientific research work produced
in the various domains dealing with the Legal and Social Medicine. As first presi
dent of the International Academy of Legal Medicine and Social Medicine, Professor
Knud Sand from Copenhagen, assisted by colleagues of the Praesidium appointed
as national representatives, succeeded in gathering together nearly the whole aca
demic people involved in Legal and Social Medicine. Thus one year later, in 1939,
The Academy became a worldwide institution of 450 members from thirty nations.
After the war, what had been before of considerable interest for the progress of the
knowledge and techniques in Legal Medicine remained again a pressing necessity
leading to the second meeting of the Academy in 1947 in Brussels under the presi
dency of Professor De Laet. Since then the meetings of the Academy followed one
another every three years. At this point, I would like to thank all the past presidents
of the Academy and in particular Professor Roche and Professor Andre for their
contribution without which the Academy would not be what it is presently. In the
meantime, it must be also admitted that the worldwide audience of the Academy at
the beginning has declined progressively year after year to become restricted to
Europeans and even to a certain extent to people from southern European nations
while scientists belonging to English speaking, German or Scandinavian countries
would rather attend the meetings of the powerful and attractive International
Association of Forensic Sciences more recently founded on the basis of a multidis
ciplinary approach of the Forensic Sciences. Even so, due to its broad spectre of
interest, the International Association of Forensic Sciences itself has to compete
now with the apparently non reversing trend towards specialization that each of us
can observe with the emergence of unidisciplinary societies or associations such as
TIAFT or IFSH which are gaining each year more in popularity and importance. In
this respect, I express the wish that the Academy could regain possession in the
domains of the Legal Medicine and sciences directly connected with the leadership
for which it has been founded more than fifty years ago. This objective does not
seem unrealistic provided that each member accept to act with full knowledge of
the case. It is the merit of Professor Enrique Villanueva to have initiated this reflec-
VI Preface
tion under his presidency. It will be without any doubt the expected role of Professor
Bernd Brinkmann, his successor, to act up the fruit of this reflection.
Legal Medicine is taught in Strasbourg for exactly two hundred years. In 1794, the
French Revolution decided to create simultaneously three Schools of Medicine in
Paris, Montpellier and Strasbourg. Immediately after its creation, the School of
Medicine of Strasbourg endowed a chair of Legal Medicine which has been occu
pied by very famous professors such as Foden!, Tourdes or Simonin. As you can see,
the Legal Medicine in Strasbourg is an old and venerable institution which succee
ded to pass through the vicissitudes of the history without losing strength but on
the contrary in drawing benefits from the alternations of the French and German
cultures. In 1990, I enjoyed the privilege of succeeding Professor Andre Chaumont
who was occupying the chair since 1962 after Professor Simonin retired. I would like
to take the opportunity which is offered to me today to express publicly to Professor
Chaumont my deep gratitude for having chosen me to take in hand the destiny of
the Institute of Legal Medicine.
Organizing this meeting was hard work and successful. I would like to express
deeply my thanks to the Praesidium of the International Academy and in particular
to its president Professor Enrique Villanueva for having chosen Strasbourg as the
seat of the XVIth Congress.
It is also a pleasure for me to express sincere appreciation to the guest speakers
and to the participants in the different scientific sessions, worshops and seminars
that should form the basic structure of the meeting. I am also grateful to my colla
borators who did a great job, to the sponsoring companies whose names are listed
on the programme book, the European Council for its support in financing the
coming of colleagues belonging to eastern European countries, the Ville de
Strasbourg, the Conseil General du Bas-Rhin and the Conseil Regional d'A lsace for
providing support and all those who helped us by some means or other.
Finally, the publication of the proceedings of the XVIth Congress constitutes the
continuation of the "Acta Medicinae Legalis" previously"et Socialis" which offers to
each membe.r of the IALM a compilation of the main scientific contributions pre
sented during this event.
Speech of Professor Giacomo Canepa
at the Opening Ceremony
of the XVIth Congress of the International Academy of Legal Medicine and Social
Medicine, Strasbourg, May 30, 1994
Messieurs les Presidents,
Mesdames, Messieurs, et Chers Collegues,
Je suis tres heureux de prendre la parole pour remercier les organisateurs de ce
a
Congres de notre Academie Internationale et pour rendre hommage tous ceux qui,
avec Ie Professeur Chaumont et Ie Professeur Mangin, ont travaille pour realiser
ceUe manifestation internationale, dont Ie programme confirme !'importance et
l'e fficacite de l' activite scientifique et pratique de notre discipline, la medecine lega
Ie.
a
Je m'adresse vous avec mes compliments tres since res et avec mes meilleurs
voeux pour Ie deroulement des travaux du Congres, non seulement en rna qualite de
vice-president du Praesidium actuel de I'Academie, mais surtout en tant que dele
gue officiel de deux Societes Internationales :
a
1) la Societe Internationale de Defense Sociale, qui doit ses origines I'initiative
d'un professeur de Droit, Filippo Gramatica, en 1947 dans rna ville de Genes et qui
a
actuellement a son siege social Paris, ou, apres la presidence de Gramatica et
d'A ncel, la Societe est actuellement presidee par Madame Simone Rozes, ancien
premier president de la Cour de Cassation. Aussi, au nom de la presidence, ainsi que
du secretaire general M. Adolfo Beria Di Argentine, je formule mes meilleurs voeux
pour Ie succes du Congres ;
2) la Societe Internationale de Criminologie, qui a son siege aP aris et dont j'a i ete
Ie president pour cinq annees (actuellement Ie president est Ie Professeur Rees de
I'Universite de Yale, USA). Le secretaire general de ceUe Societe, Ie Professeur
Georges Picca, m'a ecrit ce qui suit: "nous serions tres heureux que vous acceptiez
a
ceUe occasion, et en votre qualite de president honoraire de la Societe
Internationale de Criminologie, de representer officiellement notre organisation.
a
ainsi que vous Ie savez, nous portons en effet un vif interet l'e volution scientifique
de la Medecine Legale, qui n'est pas sans relation avec nos activites".
Les deux Societes que je represente ici, avec deux autres Societes, l' Association
Internationale de Droit Penal (President Ie Professeur Bassiouni de Chicago) et la
Fondation Internationale Penitenciaire, presidee par Ie Professeur De Figueiredo
Dias de Coimbra (on les appelle les "Quat res Soeurs"), sont dotees du statut consul
tatif aupres des Nations Unies et du Conseil de l'Europe. Nous allons preparer un
VIII Speech
rapport des Quatre Societes pour Ie "Congres de l'ONU sur la: prevention du crime
et Ie traitement des criminels", qui aura lieu a Tunis en avril 1995.
Je souhaite enfin qu'a l'avenir, notre Academie Internationale de Medecine Legale
puisse entretenir les memes liaisons tres etroites avec l'ONU et Ie Conseil de
l'Europe.
Dans ceUe perspective, je vous souhaite un bon travail et je vous remercie.
Contents
Preface of Professor Patrice MANGIN . V
Speech of Professor Giacomo CANEPA VII
1. Plenary Session
Legal Medicine in Europe . . . . . . . . . . . . . . . . . . . . . . . . . . .. 3
(A. Busuttil)
Who is Who in Forensic Sciences -
Suggestion of a Biographical Archive for Common Benefit ............... 10
(R.K. Mueller)
2. Advances in DNA technology
PCR-Based Analysis from Formalin-Fixed and Paraffin-Embedded Tissues. . . . . .. 17
(A. Alonso, P. Martin, C. Albarran, B. Aguilera, H. Oliva, and M. Sancho)
Past, Present and Future Trends in Forensic DNA Technologies in Europe ...... 20
(w. Bilr)
Evaluation of an Automated DNA-Extractor for DNA Profiling 26
(J. Bartel, W. Koegel, M. Weizenegger, and I. Pampel)
Deficiency Cases in Disputed Paternity ... 28
(c. Brandt-Casadevall and N. Dimo-Simonin)
Application of PCR to a Forensic Casework:
Identification of Burned Human Remains . . . . . . . . . . . . . . . . . . . . .. 32
(N. Cerri and F. De Ferrari)
Cadaveric DNA Extraction Protocol Based
on Cetyl Trimethyl Amonium Bromide (CTAB) ..................... 35
(D. Corach, G. Penacino, and A. SalaY
Polymarker and HLA DQA1 Genetic Markers in Forensic Casework 37
(R. Espinheira, T. Ribeiro, H. Geada, and L. Reys)
Studies on HLA DQ Alpha Genotypes carried out from Dental Material discovered
Subsequently on the Scene of the Aircrash of Flight IT 5148 . . . . . . . . . . . . . .. 39
(F. Gaillard, B. Ludes, J. M. Hutt, H. Pfitzinger, and P. Mangin)
Allele Frequencies of HLA-DQA1, LDLR, GYPA, HBGG, D7S8 and GC
in the Resident Population of the Basque Country . . . . . . . . . . . . . . . . . . .. 41
(0. Garcia, P. Martin, C. Albarran, and A. Alonso)
X Contents
HUMTHOl (TCn) and HUMFESFPS (FES)
Alleles Distribution in a South West Swiss Population ................ 44
(F. Grange, N. Dimo-Simonin, and C. Brandt-Casadevall)
Genetic Typing of Biological Evidence using PCR ....... 48
(F. Heidorn, G. Wrobel, V. Schneider, and H. Poche)
Suitability of Short Tandem Repeats in Forensics: a Comparison of 7 Loci .... . .. 52
(P. Huber and I. Holtz)
Detection of Sex Specific DNA Fingerprint bands in Peregrine Falcon
using Non Radioactive 33.6 and 33.15 Human Probes ........ ......... 55
(C. Keyser, B. Ludes, H. Pfitzinger, and P. Mangin)
Genetic Analysis of Fluorescent labeled Amplified DNA using Series
of Oligonucleotide Probes Covalently Bound to Membranes . . . . .. 58
(LA. Kozlov, N.A. Naryshkin, K. 1. Ivanov, 1. V. Lebedeva, M. G. Ivanovskaya,
and Z.A. Shabarova)
P.C.R. Amplification of D.N.A. from Amniotic Fluid
in a Paternity Study following a Rape ....... . ..... '.' ...... 60
(I.A. Lorente, M. Lorente, I.C. Alvarez, and E. Villanueva)
P.C.R. Generated Allele Frequencies in the Spanish Population ........... 62
(M. Lorente, I.A. Lorente, B. Budowle, I.e. Alvarez, M.R. Wilson, and E. Villanueva)
Positive Identification of a Couple of Flood Victims by Autopsy Data
and PCR Investigation .................................. 64
(A. Mannucci, L. Casarino, G. Bruni, A. Lomi, and F. De Stefano)
Amplified Fragment Length Polymorphism Analysis
of the VNTR Locus D1S80 in Asturias (North-West Spain) .... . . . . .. 67
(B. Martfnez-Iarreta, 1. Almuzara, R. Hinojal, and M. Castellano)
Frequency Database for the D2S44 Locus using Dig-Labeled YNH24 Probe
in the Basque Country Population . . . . . . . . . . . . . . . . . . . . . . . . .. 70
(1. Ferndndez-Ferndndez, A. Castro, S. Alonso, M. Gomez de Cedron, C. Barbero,
A. Garcia-Orad, G. Tamayo, and M. M. de Pancorbo)
Investigation of D1S80 VNTR Locus in a Polish Population 72
(D. Mikicka-Sliwka, A. Syroczynska, K. Sliwka, and I.A. Berent)
Usefulness of D1S80 System in Paternity Investigation 74
(D. MiScicka-Sliwka, K. Sliwka, A. Syroczynska, and I.A. Berent)
Detection of a New Large Allele at the D17S5 (YNZ22) Locus .............. 76
(S. Pelotti, P. Degli Esposti, V. Mantovani, E. Collina, and G. Pappalardo)
PCR Applications of Genotyping in Borderline Casework ............... 78
(A. Peneau, I.-L. Rolland, e. Tesson, I.-P. Moisan, and O. Pascal)
Use of the Amplitype PM Co amplification System on Forensic Analysis ........ 81
(M.F. Pinheiro, M.L. Pontes, and I. Pinto da Costa)
French Caucasian Population Data for HUMTHo1
and HUMFESFPS Short Tandem Repeat (STR) Systems .............. 83
(H. Pfitzinger, F. Rousselet, B. Ludes, P. Kintz, A. Tracqui, and P. Mangin)
Application of the Amplitype PM Co amplification System to Forensic Casework 85
(P. Sanz and V. Prieto)
Application of DNA Polymorphisms to Postmortem Paternity Cases ......... 87
(A. Sawaguchi, S. Nakamura, and T. Sawaguchi)
Comparison between the Consanguinity Determination
by a Multi-Locus Probe and Three Single-Locus Probes ................ 90
(L. Estevez Schwarz, E. Kattner, V. Mauer, V. Schneider, and H. Poche)
