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About 21 Cloning Simulation
The document describes a laboratory exercise simulating cloning of a jellyfish GFP gene into a bacterial plasmid. Students will cut out representations of the pUC18 plasmid and GFP gene from white and green paper, respectively. The DNA segments will be cut with the HindIII restriction enzyme, leaving "sticky ends" that allow joining the segments together using DNA ligase. This generates a recombinant plasmid that can be used to transform bacteria, allowing them to express the GFP protein and glow under black light.
Detailed Information
| Author: | ['S. Spencer'] |
|---|---|
| Publication Year: | 2008 |
| Pages: | 6 |
| Language: | English |
| Format: | |
| Price: | FREE |
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