2018 Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto ho

37 Pages·2018·8.07 MB·English

Running title: MERS-CoV and bCoV-HKU9 both utilize GRP78 for attachment

1

Title: Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both
can utilize GRP78 for attachment onto host cells

Hin Chu1,2*, Che-Man Chan1,2*, Xi Zhang2*, Yixin Wang2, Shuofeng Yuan2, Jie
Zhou1,2, Rex Kwok-Him Au-Yeung3, Kong-Hung Sze1,2, Dong Yang2, Huiping Shuai2,
Yuxin Hou2, Cun Li2, Xiaoyu Zhao2, Vincent Kwok-Man Poon2, Sze Pui Leung2,
Man-Lung Yeung1,2,4,5, Jinghua Yan6, Guangwen Lu7, Dong-Yan Jin8, George Fu
Gao6,9, Jasper Fuk-Woo Chan1,2,4,5#, Kwok-Yung Yuen1,2,4,5,10#.

Affiliations: 1 State Key Laboratory of Emerging Infectious Diseases, 2 Department of
Microbiology,
3 Department of Pathology, 4 Research Centre of Infection and

Immunology, and 5 Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine,
The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region,
China; 6CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute
of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; 7 West China
Hospital Emergency Department (WCHED), State Key Laboratory of Biotherapy,
West China Hospital, Sichuan University, and Collaborative Innovation Center of
Biotherapy, Chengdu, Sichuan 610041, China; 8School of Biomedical Sciences, Li Ka
Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong
Special Administrative Region, China; 9 National Institute for Viral Disease Control
and Prevention, Chinese Center for Disease Control and Prevention (China CDC),
Beijing 102206, China; 10 The Collaborative Innovation Center for Diagnosis and
Treatment of Infectious Diseases, Li Ka Shing Faculty of Medicine, The University of
Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.

* These authors contributed equally to this study as co-first authors.
#These authors contributed equally to this study as co-corresponding authors.

Running title: MERS-CoV and bCoV-HKU9 both utilize GRP78 for attachment
Word count: Text: 6821. Abstract: 249.
#Correspondence:
Kwok-Yung Yuen
or Jasper Fuk-Woo Chan,
State Key

Laboratory of Emerging Infectious Diseases, Carol Yu Centre for Infection,
Department of Microbiology, The University of Hong Kong, Queen Mary Hospital,
102 Pokfulam Road, Pokfulam, Hong Kong Special Administrative Region, China.
Tel: [phone]. Fax: [phone]. Email: