Table Of ContentContributors to Volume 257
Article numbers are in parentheses following the names of contributors.
Affiliations listed are current.
LLIRIK VORDNAXELA (27), Cell Biology Pro- ANNAH EKMAD (24), Department of UeC Biol-
gram, European Molecular Biology Labo- ogy, The Scripps Research Institute, La
,yrotar 69012 ,grebledieH Germany ,alloJ California 73029
SCOTt A. GNORTSMRA (5), Department of ENAITSIRHC REHCSAD (20, 21), Department
Molecular Genetics, University of Texas of lleC Biology, The Scripps hcraeseR Insti-
Southwestern Medical Center, Dallas, ,etut La Jolla, California 73029
Texas 53257
ORTEIP DE ILLIMAC (12), Department of lleC
MAILLIW E. BALCH (1, 7, ,01 20, 21), -trapeD Biology, Howard Hughes Medical ,etutitsnI
ments of Cell and Molecular Biology, The Yale University School of ,enicideM New
sppircS hcraeseR ,etutitsnI La ,alloJ -rofilaC Haven, Connecticut 01560
ain 73029
A. ARABRAB PURTSJEVS-CARID (3), Depart-
SELRAHC EWOLRAB (13), Department of Bio- ment of Biochemistry, Stanford ytisrevinU
chemistry, Dartmouth Medical School, School of Medicine, Stanford, ainrofilaC
Hanover, New Hampshire 55730 5O349
F. RALF FFOHCSIB (17), Division for -uceloM SOLRAC G. DOTTI (32), Cell Biology Pro-
ral Biology of ,sisotiM German Cancer -eR ,marg European Molecular Biology Labo-
search Center, D-69009 Heidelberg, ,yrotar D-69117 ,grebledieH Germany
ynamreG
PAUL DUPREE (32), Department of Plant -icS
MAILLIW H. KYDNORB (14, 23), Promega -roC ,secne Cambridge University, Cambridge
poration, Madison, Wisconsin 17735 2BC 3HA, United Kingdom
LEAHCIM S. NWORB (5), Department of Mo- NYLIRAM GIST RAHUQRAF (29), Division of
ralucel ,sciteneG ytisrevinU of Texas South- ralulleC and Molecular Medicine, -revinU
western Medical Center, Dallas, Texas sity of ,ainrofilaC naS Diego, La ,alloJ -ilaC
53257 fornia 39029
H. ALEX NWORB (33), Department of Phar- NASUS FERRO-NovICK (4), Department of
macology, Southwestern Medical ,retneC Cell Biology, Howard Hughes Medical In-
ytisrevinU of ,saxeT Dallas, Texas 53257 ,etutits Yale ytisrevinU School of ,enicideM
New Haven, Connecticut 01560
AILICEC BUCCI (2, 19), Dipartimento di Bio-
aigol e Patologia eralulleC e eraloceloM ENIBAS BEAIDNUERF (24), Zentrum far Mo-
"L. ,onafilaC "' 13108 Napoli, Italy lekulare Biologie red tiitisrevinU -ledieH
,greb D-69120 ,grebledieH Germany
NAMREH BUJARD (24), Zentrum far Moleku-
late Biologic red t~itisrevinU ,grebledieH DIETER ZTlWLLAG (15), Department of Mo-
02196-D ,grebledieH Germany ralucel Genetics, Max-Planck Institute for
Biophysical Chemistry, D-37018 -tOG
TENAJ L. NOTRUB (12), Department of Cell
,negnit Germany
Biology, Howard Hughes Medical ,etutitsnI
Yale University School of ,enicideM New ELLEHCIM D. rr~ERRAG (11, 26), Onyx Phar-
Haven, Connecticut 01560 ,slacituecam Richmond, California 60849
ix
X CONTRIBUTORS TO VOLUME 257
LARRY GERACE (30), Department of Cell Bi- CAROL MURPHY (34), Cell Biology Program,
ology, The Scripps Research Institute, La European Molecular Biology Laboratory,
Jolla, California 92037 D-69012 Heidelberg, Germany
JOSEPH L. NIETS~ILOG (5), Department of Mo- HIROYUKI NAKANISHI (8), Department of Mo-
lecular Genetics, University of Texas South- lecular Biology and Biochemistry, Osaka
western Medical Center, Dallas, Texas University Medical School, Suita 565, Japan
53257 AKImRO NAga~YO (6), Department of Biologi-
MANFRED GOSSEN (24), MCB Barker/Kosh- cal Sciences, Graduate School of Science,
land ASU, University of California, Berke- University of Tokyo, Tokyo 113, Japan
ley, California 02749 PETER J. NOVICK (1l, 26), Department of Cell
RONALD W. HOLZ (25), Department of Phar- Biology, Yale University School of Medi-
macology, University of Michigan Medical cine, New Haven, Connecticut 01560
School, Ann Arbor, Michigan 90184 CLAUDE NUOFFER (1, 10), Department of Cell
HISANORI HORIUCHI (2, 27), CelIBiology Pro- Biology, The Scripps Research Institute, La
gram, European Molecular Biology Labo- Jolla, California 92037
ratory, 69012 Heidelberg, Germany TOSHmIKO OKA (6), Department of Organic
Lugs A. HUBER (32), Department of Bio- Chemistry and Biochemistry, Institute of
chemistry, University of Geneva, CH-1211 Scientific and Industrial Research, Osaka
Geneva ,4 Switzerland University, Osaka 567, Japan
Yu JIANG (4), Department of Cell Biology, FRANK PETER (1, 10), Department of Cell Bi-
Howard Hughes Medical Institute, Yale ology, The Scripps Research Institute, La
University School of Medicine, New Haven, Jolla, California 92037
Connecticut 01560 SUZANNE R. PFEFVER (3, 28), Department of
RICHARD A. KAHN (16), Laboratory of Bio- Biochemistry, Stanford University School
logical Chemistry, Division of Cancer of Medicine, Stanford, California 50349
Treatment, National Cancer Institute, Na- HERWlG LGN~aSNOP (17), Division for Molec-
tional Institutes of Health, Bethesda, Mary- ular Biology of Mitosis, German Cancer
land 29802 Research Center, D-69009 Heidelberg,
AKIRA KIKUCHI (8), Department of Biochem- Germany
istry, Hiroshima University School of Medi- PAUL A. RANDAZZO (16), Laboratory of Bio-
cine, Hiroshima 734, Japan logical Chemistry, Division of Cancer
KEITaROU KIMU~ (6), Genetics Engineering Treatment, National Cancer Institute, Na-
Laboratory, National Food Research Insti- tional Institutes of Health, Bethesda, Mary-
tute, Tsukuba 305, Japan land 29802
IAN G. MaCARA (14, 23), Department of Pa- MARKUS A. RmDERER (3), Department of
thology, University of Vermont, Burlington, Biochemistry, Stanford University School
Vermont 50450 of Medicine, Stanford, California 50349
Luis MARTIN-PARRAS (22), Cell Biology Pro- DENISE M. ROBERTS (11), Department of Cell
gram, European Molecular Biology Labo- Biology, Yale University School of Medi-
ratory, D-69117 Heidelberg, Germany cine, New Haven, Connecticut 01560
J. MICHAEL MCCAFFERY (29), Division of GUENDALINA ROSSI (4), Department of Cell
Cellular and Molecular Medicine, Univer- Biology, Howard Hughes Medical Institute,
sity of California, San Diego, LaJolla, Cali- Yale University School of Medicine, New
fornia 39029 Haven, Connecticut 01560
FRAUKE MELCHIOR (30), Department of Cell TONY ROWE (7), Department of Cell Biology,
Biology, The Scripps Research Institute, La The Scripps Research Institute, La Jolla,
Jolla, California 92037 California 92037
CONTRIBUTORS TO VOLUME 257 xi
AYUKAT IKASAS (9), Department of Molecu- University Medical School and Department
lar Biology and Biochemistry, Osaka Uni- of Cell Physiology, National Institute for
versity Medical School, Suita 565, Japan Physiological Sciences, Suita, Osaka ,565
ELLEBASI KLAHCS (10), Department of Cell Japan
Biology, The Scripps Research Institute, La LERUAL SAMOHT (21), Vollum Institute, Ore-
Jolla, California 92037 gon Health Sciences University, Portland,
YDNAR NAIv/KEHCS (13, 18), Departments of Oregon 10279
Molecular and Cell Biology, Howard
YRAG SAMOHT (21), VoUum Institute, Oregon
Hughes Medical Institute, University of Health Sciences University, Portland, Ore-
California, Berkeley, Berkeley, California gon 10279
02749
NELLE J. ELADSIT (20), Department of Cell
ARDNAS L. DIMHCS (24), Department of Cell
Biology, The Scripps Research Institute, La
Biology, The Scripps Research Institute, La
Jolla, California 92037
Jolla, California 92037
LEAHCIM D. RELHU (25), Department of Bio-
LEUGIM C. ARBAES (5), Department of Molec-
logical Chemistry and The Mental Health
ular Genetics, University of Texas South-
Research Institute, University of Michigan
western Medical Center, Dallas, Texas
Medical School, Ann Arbor, Michigan
53257
90184
HTUR A. RETNES (25), Department of Phar-
macology, University of Michigan Medical REVILO HCIRLLU (2, 27), Cell Biology Pro-
School, Ann Arbor, Michigan 48109 gram, European Molecular Biology Labo-
ratory, 69012 Heidelberg, Germany
NALLA D. ORIPAHS (28), Department of Bio-
chemistry, Stanford University School of YDUJ K. EKYLSNAV (21), Vollum Institute,
Medicine, Stanford, California 50349 Oregon Health Sciences University, Port-
land, Oregon 10279
IHCIMORIH IKATARIHS (31), Department of
Cell Biology, National Institute for Physio- ARTEP REMLLOV (15), Department of Molecu-
logical Sciences, Okazaki 444, Japan lar Genetics, Max Planck Institute for Bio-
YRREIHT ITADLOS (3, 28), Department of Bio- physical Chemistry, D-37018 Gottingen,
chemistry, Stanford University School of Germany
Medicine, Stanford, California 50349 ARFO SSIEW (16), Department of Endocrinol-
DLARAH KRAMNETS (19), Cell Biology Pro- ogy and Metabolism, Hadassah University
gram, European Molecular Biology Labo- Hospital, Jerusalem ,02119 Israel
ratory, D-69012 Heidelberg, Germany SAMOHT GNUEY (18), Division of Biochemis-
PAUL C. SIEWNRETS (33), Department of try and Molecular Biology, Howard Hughes
Pharmacology, University of Texas South- Medical Institute, University of California,
western Medical Center, Dallas, Texas Berkeley, Berkeley, California 02749
53257
URHOT ASIHIHSOY (18), Department of Cell
HAROBED J. TEEWS (30), Department of Cell Biology, Institute for Virus Research, Kyoto
Biology, The Scripps Research Institute, La University, Kyoto ,10-606 Japan
Jolla, California 92037 ONIRAM LAIREZ (2, 19, 22, 27, 34), Cell Biol-
IMIHSOY IAKAT (8, 9, 31), Department of Mo- ogy Program, European Molecular Biology
lecular Biology and Biochemistry, Osaka Laboratory, D-69012 Heidelberg, Germany
Preface
GTPases are now recognized as essential components for protein traffic
between all compartments of the cell. This includes vesicular traffic through
the exocytic and endocytic pathways, where GTPases play key roles in the
assembly of vesicle coats (budding), in vesicle targeting and in fusion, as
well as in protein traffic in and out of the nucleus. GTPases involved in
transport include the Rab and ARF families, Sarl, Ran, dynamin, and
heterotrimeric G proteins. In addition to GTPase, a number of associated
accessory factors are critical for function. These include posttranslational
modifying enzymes (such as prenyl transferases and myristyl transferases),
factors that affect guanine nucleotide binding [guanine nucleotide dissocia-
tion inhibitors (GDIs) and guanine nucleotide exchange factors (GEFs)],
and factors that stimulate guanine nucleotide hydrolysis [GTPase-activating
proteins (GAPs)].
To understand the function of GTPases and their cognate factors, a
wealth of in vitro biochemical and in vivo molecular genetic approaches are
currently being applied to individual proteins. Given the diverse spectrum of
compartments regulated by individual GTPases, techniques developed for
one particular member of a family are often applicable to other members.
In a broader sense, many of the techniques developed for a particular
gene family are also frequently applicable to other gene families given the
exceptional structural configuration of GTPases.
The purpose of this volume is to bring together the latest technologies
in the study of GTPase function involved in protein trafficking. It provides
concise descriptions of the recent methodological innovations that allow
both the novice and experienced investigator to explore the function of
these proteins in detail. We are extremely grateful to the many investigators
who have generously contributed their time and expertise to bring this
wealth of technical experience to one volume. It should provide a valuable
resource to address the many issues confronting our understanding of the
role of these GTPases in the biology of cell.
W. E. BALCH
GNINNAHC J. DER
HALL ALAN
xiii
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV II. Preparation and Assay of Enzymes
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV III. Preparation and Assay of Substrates
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV IV. Special Techniques for the Enzymologist
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV V. Preparation and Assay of Enzymes
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV VI. Preparation and Assay of Enzymes (Continued)
Preparation and Assay of Substrates
Special Techniques
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV VII. Cumulative Subject Index
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
EMULOV VIII. Complex Carbohydrates
Edited by ELIZABETH F. NEUFELD DNA VICTOR GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited by WILLIS A. WOOD
EMULOV X. Oxidation and Phosphorylation
Edited by RONALD W. KOORBATSE DNA MAYNARD E. NAMLLUP
EMULOV XI. Enzyme Structure
Edited by C. H. W. HIRS
EMULOV XII. Nucleic Acids (Parts A and B)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XIII. Citric Acid Cycle
Edited by J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited yb J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited by DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
Edited by HTENNEK NITSUK
XV
XVi METHODS IN ENZYMOLOGY
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited yb TREBREH ROBAT DNA CELIA ETIHW ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited by DLANOD B. KCIMRoCcM DNA LEUMEL D. THGIRW
EMULOV XIX, Proteolytic Enzymes
Edited by EDURTREG E. NNAMLREP DNA OLZSAL DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited yb MAILLIW B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited yb YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited yb YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited by .C H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXII. Biomembranes (Part )B
Edited by SIDNEY FLEISCHER AND LESTER PACKER
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by AHTRAM G. SINNED DNA DRAWDE A. SINNED
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited by MAILLIW B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XXXV, Lipids (Part B)
by Edited NHOJ .M NIETSNEWOL
METHODS IN ENZYMOLOGY xvii
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited by BERT W. O'MALLEY AriD JOEL G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited by JOEL G. HARDMAN AND BERT W. O'MALLEY
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and Organ
Systems)
Edited by JOEL G. HARDMAN AND BERT W. O'MALLEY
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by BERT W. O'MALLEY AND JOEL G. HARDMAN
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited by W. A. DOOW
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited by W. A. DOOW
EMULOV XLIII. Antibiotics
Edited by JOHN H. HASH
EMULOV XLIV. Immobilized Enzymes
Edited by SUALK HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited by OLZSAL DNAROL
EMULOV XLVI. Affinity Labeling
Edited by WILLIAM B. JAKOBY AND MEIR WILCHEK
EMULOV XLVII. Enzyme Structure (Part E)
Edited by C. H. W. HIRS DNA EGRES N. FEEHSAMIT
EMULOV XLVIII. Enzyme Structure (Part F)
Edited by C. H, W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLIX. Enzyme Structure (Part G)
Edited by .C H, W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV L. Complex Carbohydrates (Part C)
Edited by ROTCIV GRUBSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited by PATRICIA A. HOFFEE AND MARY ELLEN JONES
EMULOV LII. Biomembranes (Part C: Biological Oxidations)
Edited by SIDNEY FLEISCHER AND LESTER PACKER
EMULOV LIII. Biomembranes (Part D: Biological Oxidations)
Edited by SIDNEY FLEISCHER AND LESTER PACKER
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited by SIDNI~Y FLEISCHER AND LESTER PACKER
xviii METHODS NI ENZYMOLOGY
EMULOV LV. Biomembranes (Part F: Bioenergetics)
Edited by SIDNEY FLEISCHER DNA tESTER PACKER
EMULOV LVI. Biomembranes (Part G: Bioenergetics)
Edited by SIDNEY FLEISCHER DNA tESTER PACKER
EMULOV LVII. Bioluminescence and Chemiluminescence
Edited by ENELRAM A. DELUCA
EMULOV LVIII. Cell Culture
Edited by WILLIAM B. JAKOBY DNA IRA PASTAN
EMULOV LIX. Nucleic Acids and Protein Synthesis (Part G)
Edited by KIVIE MOLDAVE DNA LAWRENCE GROSSMAN
EMULOV LX, Nucleic Acids and Protein Synthesis (Part H)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV .16 Enzyme Structure (Part H)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV .26 Vitamins and Coenzymes (Part D)
Edited by DLANOD B. McCoRMICK DNA LEMUEL D, WRIGHT
EMULOV .36 Enzyme Kinetics and Mechanism (Part A: Initial Rate and Inhibitor
Methods)
Edited by DANIEL n. PURICH
EMULOV .46 Enzyme Kinetics and Mechanism (Part B: Isotopic Probes and Com-
plex Enzyme Systems)
Edited by DANIEL L. HCIRUP
EMULOV .56 Nucleic Acids (Part I)
Edited by LAWRENCE GROSSMAN DNA KIVIE MOLDAVE
EMULOV .66 Vitamins and Coenzymes (Part E)
Edited by DLANOD B. McCoRMICK DNA LEUMEL D. WRIGHT
EMULOV .76 Vitamins and Coenzymes (Part F)
Edited by DLANOD B. McCoRMICK DNA LEUMEL D. WRIGHT
EMULOV .86 Recombinant DNA
Edited by YAR Wu
EMULOV .96 Photosynthesis and Nitrogen Fixation (Part C)
Edited by YNOHTNA NAS ORTEIP
EMULOV .07 Immunochemical Techniques (Part A)
Edited by HELEN NAV SIKANUV DNA JOHN J. ENOGNAL
EMULOV .17 Lipids (Part C)
Edited by JOHN M. NIETSNEWOL
EMULOV .27 Lipids (Part D)
Edited by JOHN M. NIETSNEWOL
EMULOV .37 Immunochemical Techniques (Part B)
Edited by JOHN J. ENOGNAL DNA HELEN NAV SIKANUV
SDOHTEM NI YGOLOMYZNE xix
EMULOV .47 Immunochemical Techniques (Part C)
Edited by JOHN J. LANGONE DNA HELEN VAN SIKANUV
EMULOV 75. Cumulative Subject Index Volumes XXXI, XXXII, XXXIV-LX
Edited by EDWARD A. DENNIS DNA MARTHA G. DENNIS
EMULOV 76. Hemoglobins
Edited by ERALDO ANTONINI, LUIGI ROSSI-BERNARDI, DNA EMILIA ENOCNAIHC
EMULOV .77 Detoxication and Drug Metabolism
Edited by WILLIAM B. YBOKAJ
EMULOV .87 Interferons (Part A)
Edited by YENDIS AKTSEP
EMULOV .97 Interferons (Part B)
Edited by YENDIS AKTSEP
EMULOV .08 Proteolytic Enzymes (Part C)
Edited by OLZSAL DNAROL
EMULOV .18 Biomembranes (Part H: Visual Pigments and Purple Mem-
branes, I)
Edited by RETSEL REKCAP
EMULOV .28 Structural and Contractile Proteins (Part A: ExtraceUular Matrix)
Edited by NOEL W. MAHGNINNUC DNA DIXIE W. NESKIREDERF
EMULOV .38 Complex Carbohydrates (Part D)
Edited by ROTCIV GRUBSNIG
EMULOV .48 Immunochemical Techniques (Part D: Selected Immunoassays)
Edited by JortN J. ENOGNAL DNA NELEH NAV SIKANUV
EMULOV .58 Structural and Contractile Proteins (Part B: The Contractile Appara-
tus and the Cytoskeleton)
Edited by DIXIE W. NESKIREDERF DNA LEON W. MAHGNINNUC
EMULOV .68 Prostaglandins and Arachidonate Metabolites
Edited by WILLIAM E. M. SDNAL DNA WILLIAM L. HTIMS
EMULOV .78 Enzyme Kinetics and Mechanism (Part C: Intermediates, Stereo-
chemistry, and Rate Studies)
Edited by DANIEL L. PURICH
EMULOV .88 Biomembranes (Part I: Visual Pigments and Purple Mem-
branes, II)
Edited by RETSEL REKCAP
EMULOV .98 Carbohydrate Metabolism (Part D)
Edited by WILLIS A. WOOD
EMULOV .09 Carbohydrate Metabolism (Part E)
Edited by WILLIS A. WOOD
EMULOV .19 Enzyme Structure (Part I)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
XX METHODS IN ENZYMOLOGY
EMULOV .29 Immunochemical Techniques (Part E: Monoclonal Antibodies and
General Immunoassay Methods)
Edited by NHOJ J. LANGONE AND HELEN VAN VUNAKIS
EMULOV .39 Immunochemical Techniques (Part F: Conventional Antibodies, Fc
Receptors, and Cytotoxicity)
Edited by NHOJ J. LANGONE AND HELEN VAN VUNAK1S
EMULOV .49 Polyamines
Edited by HERBERT TABOR AND CELIA WHITE TABOR
EMULOV .59 Cumulative Subject Index Volumes 61-74, 76-80
Edited by EDWARD A. DENNIS AND MARTHA G. DENNIS
EMULOV .69 Biomembranes Part J: Membrane Biogenesis: Assembly and Tar-
geting (General Methods; Eukaryotes)
Edited by YENDIS FLEISCHER AND BECCA FLEISCHER
EMULOV .79 Biomembranes Part K: Membrane Biogenesis: Assembly and Tar-
geting (Prokaryotes, Mitochondria, and Chloroplasts)
Edited by YENDIS FLEISCHER AND BECCA FLEISCHER
EMULOV .89 Biomembranes (Part L: Membrane Biogenesis: Processing and Re-
cycling)
Edited by SIDNEY FLEISCHER AND BECCA FLEISCHER
EMULOV .99 Hormone Action (Part F: Protein Kinases)
Edited by JACKIE D. CORBIN AND JOEL G. HARDMAN
EMULOV 100. Recombinant DNA (Part B)
Edited by YAR Wu, LAWRENCE GROSSMAN, AND KIVIE MOLDAVE
EMULOV 101. Recombinant DNA (Part C)
Edited by RAY Wu, LAWRENCE GROSSMAN, AND KIVIE MOLDAVE
EMULOV 102. Hormone Action (Part G: Calmodulin and Calcium-Binding Pro-
teins)
Edited by ANTHONY R. MEANS AND BERT W. O'MALEEY
EMULOV 103. Hormone Action (Part H: Neuroendocrine Peptides)
Edited by P. LEAHCIM NNOC
EMULOV 104. Enzyme Purification and Related Techniques (Part C)
Editedby WILLIAM B. JAKOBY
EMULOV 105. Oxygen Radicals in Biological Systems
Edited by RETSEL REKCAP
EMULOV 106. Posttranslational Modifications (Part A)
Edited by FINN WOLD AND KIVIE MOLDAVE
EMULOV 107. Posttranslational Modifications (Part B)
Edited by FINN WOLD AND KIVIE MOLDAVE
EMULOV 108. Immunochemical Techniques (Part G: Separation and Characteriza-
tion of Lymphoid Cells)
Edited by GIOVANNI DI SABATO, JOHN J. LANGONE, AND
HELEN VAN VUNAKIS
