Table Of ContentSingle-Molecule Science
Single-moleculescience(SMS)hasemergedfromdeveloping,using,andcombin-
ing technologies such as super-resolution microscopy, atomic force microscopy,
and optical and magnetic tweezers, alongside sophisticated computational and
modeling techniques. This comprehensive, edited volume brings together
authoritative overviews of these methods from a biological perspective, and
highlightshowtheycanbeusedtoobserveandtrackindividualmoleculesand
monitor molecularinteractionsinlivingcells.Pioneersinthisfast-moving field
cover topics such as single-molecule optical maps, nanomachines, and protein
folding and dynamics. A particular emphasis is also given to mapping DNA
moleculesfordiagnosticpurposes,andthestudyofgeneexpression.Withnumer-
ousillustrations,thisbookrevealshowSMShaspresenteduswithanewwayof
understanding life processes. It is a must-have for researchers and graduate stu-
dents,aswellasthoseworkinginindustry,primarilyinthe areasof biophysics,
biologicalimaging,genomics,andstructuralbiology.
KRISHNARAOAPPASANI isanaward-winningscientistandthefounderandchief
executive officer of GeneExpression Systems, a global conference-producing
organizationfocusingonbiomedicalandphysicalsciences.Heisalsoanelected
lifefellowoftheRoyalSocietyofBiologists,London,UK.
RAGHU KIRAN APPASANI isapsychiatrist,andneuroscientist.Heistherecipient
oftheLeonardTowHumanisminMedicineAward.Heisalsothefounderand
chiefexecutiveofficerofTheMINDSFoundation.
Published online by Cambridge University Press
Published online by Cambridge University Press
Single-Molecule Science
FROMSUPER-RESOLUTIONMICROSCOPYTODNAMAPPING
AND DIAGNOSTICS
Edited by
Krishnarao Appasani
GeneExpressionSystems,Inc.
Raghu Kiran Appasani
TheMINDSFoundation,Boston,Massachusetts,USA
Foreword by
Manfred Auer
TheUniversityofEdinburgh,Edinburgh,UnitedKingdom
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www.cambridge.org
Informationonthistitle:www.cambridge.org/9781108423366
DOI:10.1017/9781108525909
©CambridgeUniversityPress2022
Thispublicationisincopyright.Subjecttostatutoryexception
andtotheprovisionsofrelevantcollectivelicensingagreements,
noreproductionofanypartmaytakeplacewithoutthewritten
permissionofCambridgeUniversityPress.
Firstpublished2022
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ISBN978-1-108-42336-6Hardback
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ofURLsforexternalorthird-partyinternetwebsitesreferredtointhispublication
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accurateorappropriate.
Published online by Cambridge University Press
Dedicated to
TomylatefriendArthurBeckPardee(1921–2019),an
American-born legendary biochemist/molecular biologist/
cancer biologist,whois famous for his part in the PaJoMo
experimentin late 1950s, which greatly helped in the
discovery of messenger RNA; discovered the restriction
pointin early 1970sin which a cell commits itself to a
certaincellcycle;didagreatdealofworkinunderstanding
tumor growth and regulation during 1980s;and
codiscovered differential display methodology in
early 1990s.
Published online by Cambridge University Press
Published online by Cambridge University Press
Contents
ListofContributors pageix
ForewordbyManfredAuer xiv
Preface xix
PartI Super-ResolutionMicroscopyandMolecularImagingTechniques
toProbeBiology 1
1 IntroductiononSingle-MoleculeScience 3
KrishnaraoAppasaniandRaghuK.Appasani
2 OneMolecule,TwoMolecules,RedMolecules,BlueMolecules:Methods
forQuantifyingLocalizationMicroscopyData 20
GaetanG.HerbomelandGeorgeH.Patterson
3 MultiscaleFluorescenceImaging 38
ManuelGunkel,JanPhilippEberle,RubenBulkescher,JürgenReymann,
InnChung,RonaldSimon,GuidoSauter,VytauteStarkuviene,
KarstenRippe,andHolgerErfle
4 Long-ReadSingle-MoleculeOpticalMaps 49
AssafGrunwald,YaelMichaeli,andYuvalEbenstein
PartII ProteinFolding,Structure,Confirmation,andDynamics 65
5 Single-MoleculeMechanicsofProteinNanomachines 67
(cid:1)
GabrielZoldákandKatarzynaTych
6 PosttranslationalProteinTranslocationthroughMembranesatthe
Single-MoleculeLevel 80
DiegoQuiroga-Roger,HildaM.Alfaro-Valdés,andChristianA.M.Wilson
PartIII MappingDNAMoleculesattheSingle-MoleculeLevel 95
7 ObservingDynamicStatesofSingle-MoleculeDNAandProteinsUsing
AtomicForceMicroscope 97
JingqiangLi,SitharaWijeratne,andChing-HwaKiang
vii
Published online by Cambridge University Press
viii Contents
8 AtomicForceMicroscopyandDetectingaDNABiomarkerofaFew
CopieswithoutAmplification 111
SouravMishra,YoonheeLee,andJoonWonPark
PartIV Single-MoleculeBiologytoStudyGeneExpression 125
9 Single-MoleculeDetectionintheStudyofGeneExpression 127
VipinKumar,SimonLeclerc,andYuichiTaniguchi
Index 142
Published online by Cambridge University Press
Contributors
HildaM.Alfaro-Valdés
DepartmentofBiochemistryandMolecularBiology
FacultyofChemicalSciencesandPharmacy
UniversityofChile
Santiago,Chile
KrishnaraoAppasani
GeneExpressionSystems,Inc.
Waltham,MA,USA
RaghuKiranAppasani
MINDSFoundationUSAandIndia
Boston,MA,USA
RubenBulkescher
AdvancedBiologicalScreeningFacility
BioQuant,HeidelbergUniversity
Heidelberg,Germany
InnChung
UniversityofHeidelberg
BIOQUANT,GermanCancerResearchCenter(DKFZ)&
Heidelberg,Germany
YuvalEbenstein
DepartmentofChemicalPhysics
SchoolofChemistry
TelAvivUniversity
TelAviv,Israel
ix
Published online by Cambridge University Press
x ListofContributors
JanPhilippEberle
AdvancedBiologicalScreeningFacility
BioQuant,HeidelbergUniversity
Heidelberg,Germany
HolgerErfle
HeadoftheAdvancedBiologicalScreeningFacility
BioQuant,HeidelbergUniversity
Heidelberg,Germany
AssafGrunwald
DepartmentofChemicalPhysics
SchoolofChemistry
TelAvivUniversity
TelAviv,Israel
ManuelGunkel
AdvancedBiologicalScreeningFacility
BioQuant,HeidelbergUniversity
Heidelberg,Germany
GaetanG.Herbomel
SectionofBiophotonics
NationalInstituteofBiomedicalImagingandBioengineering(NIBIB)
NationalInstitutesofHealth
Bethesda,MD,USA
Ching-HwaKiang
DepartmentofPhysicsandAstronomy
RiceUniversity
Houston,TX,USA
VipinKumar
LaboratoryforSingleCellGeneDynamics
QuantitativeBiologyCenter
RIKEN
Suita,Osaka,Japan
SimonLeclerc
LaboratoryforSingleCellGeneDynamics,QuantitativeBiologyCenter,
RIKEN
Suita,Osaka,Japan
Published online by Cambridge University Press
