Recombinant protein production from plants : production and isolation of clinically useful compounds

309 Pages·1998·30.336 MB·English

by  Charles Cunningham, Andrew J. R. Porter

Recombinant Proteins from Plants
METHODS IN BIOTECHNOLOGY
TM
John M. Walker, SEIRES ROTIDE
3. Recombinant Proteins from Plants: Production and Isolation
of Clinically Useful Compounds, edited by Charles
Cunningham and Andrew J. .R Porter, 1998
2. Protocols in Bioremediation, edited by David Sheehan, 1997
.1 Immobilization of Enzymes and Cells, edited by Gordon F.
Bickerstaff 1997
Recombinant
Proteins from Plants
Production and Isolation
of Clinically Useful Compounds
detidE by
Charles Cunningham
and Andrew J. R. Porter
ytisrevinU of ,needrebA Scotland, KU
Humana Press ~E~ ,awotoT weN yesreJ
© 1998 Humana Press Inc.
999 Riverview Drive, Suite 208
Totowa, New Jersey 07512
All rights reserved. No part of this book may be reproduced, stored in a retrieval system, or transmitted in
any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise
without written permission from the Publisher. Methods in Biotechnology Mr is a trademark of The Humana
Press Inc.
All authored papers, comments, opinions, conclusions, or recommendations are those of the author(s), and
do not necessarily reflect the views of the publisher.
This publication is printed on acid-free paper.
ANSI Z39.48-1984 (American Standards Institute) Permanence of Paper for Printed Library Materials.
Cover illustration: Fig. 3B from Chapter 9, "Single-Chain Fv Antibodies Expressed in Plants," by Udo
Conrad, Ulrike Fiedler, Olga Artsaenko, and Julian Phillips.
Cover design by Patricia F. Cleary.
For additional copies, pricing for bulk purchases, and/or information about other Humana titles, contact
Humana at the above address or at any of the following numbers: Tel.: [phone]; Fax: [phone] ;
E-mait: [email protected]; Website: http://humanapress.com
Photocopy Authorization Policy:
Authorization to photocopy items for internal or personal use, or the internal or personal use of specific
clients, is granted by Humana Press Inc., provided that the base fee of US $8.00 per copy, plus US $00.25
per page, is paid directly to the Copyright Clearance Center at 222 Rosewood Drive, Danvers, MA 01923.
For those organizations that have been granted a photocopy license from the CCC, a separate system of
payment has been arranged and is acceptable to Humana Press Inc. The fee code for users of the Transactional
Reporting Service is: 0-89603-390-2/98 $8.00 + $00.25].
Printed in the United States of America. l0 9 8 7 6 5 4 3 2 I
Library of Congress Cataloging in Publication Data
Main entry under title:
Methods in biotechnolology .MT
Recombinant proteins from plants / edited by Charles Cunningham and Andrew J. R. Porter.
p. cm. -- (Methods in biotechnolology~; 3)
Includes index.
ISBN 0-89603-390-2 (alk. paper)
.1 Plant biotechnology--Laboratory manuals. 2. Recombinant proteins--Laboratory manuals.
I. Cunningham, Charles. .1I Porter, Andrew J.R. II. Series.
TP248.27.P55R43 1998
660.6'3--dc21 97-35760
CIP
Preface
Recombinant Proteins from Plants is one of the most exciting and fastest
developing areas in biology. The latest molecular techniques are being
applied to the exploitation of plants as novel expression systems for the pro-
duction and overproduction of heterologous and native proteins. Transgenic
plant technology is currently used in three broad areas: the expression of re-
combinant proteins to improve crop quality by increasing disease/pest resis-
tance or increasing tolerance to stress, optimizing plant productivity and yield
by the genetic manipulation of metabolic pathways, and the large-scale cost-
effective production of recombinant proteins for use as specialist industrial or
therapeutic biomolecules.
The intention of Recombinant Proteins from Plants is to provide com-
prehensive and detailed protocols covering all the latest molecular approaches.
Because the production oftransgenic plants has become routine in many labo-
ratories, coverage is also given to some of the more "classical" approaches to
the separation, analysis, and characterization of recombinant proteins. The
book also includes areas of research that we believe will become increasingly
important in the near future: efficient transformation of monocots with
Agrobacterium ,sneicafimut optimizing the stability of recombinant proteins,
and a section highlighting the immunotherapeutic potential of plant-expressed
proteins.
The contents bring together a broad range of knowledge and expertise from
around the world; each chapter is written by research workers with hands-on
experience of the protocols they describe. The book opens with chapters cov-
ering the introduction of transgenes into monocot and dicot species, and
includes the expression and characterization of reporter genes and the appli-
cation of recombinant proteins in crop protection. This is followed by sections
that describe the expression of cytokines, antibodies/antibody fragments, and
use of recombinant plant viral particles as vaccines. The later part of the book
deals with the expression of proteins by plant suspension culture/protoplasts,
explores the latest strategies to increase the yield of recombinant proteins by
controlling the cellular location of protein folding, and the current protocols
available for the molecular manipulation of photosynthetic pathways. The
final chapters of the book outline techniques specifically designed to quantify
V
vi Preface
and characterize plant-derived proteins and include: glycosylation state, ELISA,
and two-dimensional electrophoresis.
We hope that we have provided a detailed handbook of essential
approaches and methodologies that will be of benefit both to people new to
plant protein biotechnology and to established laboratories in the field.
Charles Cunningham
Andrew J. R. Porter
Contents
Preface ............................................................................................................. v
Contributors ..................................................................................................... ix
1 Rice Transformation by Agrobacterium Infection,
Xiongying Cheng, Ravinder K. Sardana, and Illimar Altosaar ....... I
2 Barley as a Producer of Heterologous Protein,
Leena Mannonen, Kristian Aspegren, Annefi Ritala,
Hanna Simola, and Teemu H. Teeri .............................................. 11
3 Introducing and Expressing Genes in Legumes,
Adrian D. Bavage, Mark P. Robbins, Leif Skit,
and K. Judith Webb ........................................................................ 27
4 Use of the GUS Reporter Gene,
Joy Wilkinson and Keith Lindsey ..................................................... 39
5 Expression of Recombinant Proteinase Inhibitors in Plants,
Dominique Michaud and Thierry .C Vrain ....................................... 49
6 Detection of Recombinant Viral Coat Protein in Transgenic Plants,
Herta Steinkellner and Irina Korschhineck ..................................... 65
7 Synthesis of Recombinant Human Cytokine GM-CSF
in the Seeds of Transgenic Tobacco Plants,
Ravinder K. Sardana, Peter .R Ganz, Anil Dudani,
Eilleen .S Tackaberry, Xiongying Cheng,
and Illimar Altosaar ........................................................................ 77
8 Tobamovirus Vectors for Expression of Recombinant Genes
in Plants,
Thomas .H Turpen and Stephen .J Reinl ......................................... 89
9 Single-Chain Fv Antibodies Expressed in Plants,
Udo Conrad, Ulrike Fiedler, Olga Artsaenko,
and Julian Phillips ........................................................................ 301
10 Characterization and Applications of Plant-Derived Recombinant
Antibodies,
Rainer Fischer, J~rgen Drossard, Yu-Cai Liao,
and Stefan Schillberg .................................................................. 129
v//
viii Contents
11 Production of Recombinant Antibodies
ni Plant Suspension Cultures,
JBrgen Drossard, Yu-Cai Liao, and Rainer Fischer ..................... 143
21 Production of Foreign Proteins
ni Tobacco Cell Suspension Culture,
Wronique Gomord, Anne-Catherine Fitchette-Laine
Lise-Anne Denmat, Dominique Michaud, and Lo'ic Faye ....... 155
31 Transient Gene Expression ni Plant Protoplasts,
Stefan Schillberg, Sabine Zimmermann, Dirk PrBfer,
Detlef Schuman, and Rainer Fischer ......................................... 165
41 Stability of Recombinant Proteins ni Plants,
Dominique Michaud, Thierry .C Vrain, Veronique Gomord,
and LoTc Faye ................................................................................ 177
51 Trafficking and Stability of Heterologous Proteins
ni Transgenic Plants,
Johnathan A. Napier, Gaelle Richard, and Peter .R Shewry ....... 189
61 Screening for Transgenic Lines with Stable
and Suitable Accumulation Levels of a Heterologous Protein,
Myriam De Neve, Helena naV Houdt, Anne-Marie Bruyns,
Marc naV Montagu, and Ann Depicker ...................................... 203
71 Manipulation of Photosynthetic Metabolism,
Martin A. J. Parry, Steven .P Colliver, Pippa J. Madgwick,
and Matthew J. Paul ..................................................................... 229
81 Quantification of Heterologous Protein Levels
ni Transgenic Plants by ELISA,
Anne-Marie Bruyns, Myriam De Neve, Geert De Jaeger,
Chris De Wilde, Pierre Rouze, and Ann Depicker .................... 251
91 Analysis of -N and O-Glycosylation of Plant Proteins,
Anne-Catherine Fitchette-Laine, Lise-Anne Denmat,
Patrice Lerouge, and LoTc Faye .................................................. 271
20 Two-Dimensional Electrophoresis: A Too/for Protein Separation
and Processing for Microsequencing,
N.P. Eswara Reddy and .M Jacobs ................................................. 291
Index ............................................................................................................ 305
Contributors
RAMILL] RAASOTLA Department of Biochemistry, University of Ottawa,
°
Ottawa, Ontario, Canada
AGLO OKNEASTRA IPK Gatersleben, Gatersleben, Germany
°
NAITSIRK NERGEPSA VTT Biotechnology and Food Research, Finland
°
NAIRDA .D °EGAVAB Department of Brassica and Oilseeds Research,
John Innes Centre, Norwich, UK
EIRAM-ENNA SNYURB Laboratorium voor Genetica, Department
°
of Genetics, Flanders Interuniversity Institute for Biotechnology,
University of Gent, Belgium
GNIYGNOIX GNENC Department of Biochemistry, University of Ottawa,
°
Ottawa, Ontario, Canada
NEVETS .P REVILLOC Biochemistry and Physiology Department, IACR-
•
Rothamsted, Harpenden, UK
ogU DARNOC IPK Gatersleben, Gatersleben, Germany
•
maELG ED REGEAJ Department of Genetics, Flanders Interuniversity
°
Institute for Biotechnology, University of Gent, Belgium
MAIRYM ED EVEN Department of Genetics, Flanders Interuniversity
°
Institute for Biotechnology, University of Gent, Belgium
SIRHC ED EDLIW Department of Genetics, Flanders Interuniversity
°
Institute for Biotechnology, University of Gent, Belgium
ENNA-ESIL TAMNED Laboratoire des Transports Intracellulaires,
°
Universite de Rouen, Facult6 des Sciences, Mont-Saint-Aigen,
France
NNA REKCIPED Department of Genetics, Flanders Interuniversity
•
Institute for Biotechnology, University of Gent, Belgium
NEGROJ DRASSORD Institut fur Biologie I (Botanik/Molekulargenetik),
°
Rheinisch- ehcsilaftseW Technische Hochschule Aachen, Germany
LINA INADUD Ottawa Blood Centre, CRCS, Ottawa, Ontario, Canada
•
cfoL EYAF Laboratoire des Transports Intracellulaires, Universit6 de
•
Rouen, Faculte des Sciences, Mont-Saint-Aigen, France
EKIRLU RELDEIF IPK Gatersleben, Gatersleben, Germany
°
ix