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Plant virology protocols: from virus isolation to transgenic resistance PDF

508 Pages·1998·32.808 MB·English
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by Sally C. Taylor| 1998| 508 pages| 32.808| English

About Plant virology protocols: from virus isolation to transgenic resistance

A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.

Detailed Information

Author:Sally C. Taylor
Publication Year:1998
ISBN:9780896033856
Pages:508
Language:English
File Size:32.808
Format:PDF
Price:FREE
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