Plant Cell Culture Protocols (Methods in Molecular Biology

411 Pages·2007·3.54 MB·English

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Plant Cell Culture Protocols
M E T H O D S I N M O L E C U L A R B I O L O G Y™
John M. Walker, SERIES EDITOR
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M E T H O D S I N M O L E C U L A R B I O L O G Y™
Plant Cell Culture
Protocols
Second Edition
Edited by
Victor M. Loyola-Vargas
and
Felipe Vázquez-Flota
Unidad de Bioquímica y Biología Molecular de Plantas,
Centro de Investigacion Cientifica de Yucatán, Yucatán, México
© 2006 Humana Press Inc.
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Cover illustration: Cover illustration: Figure 3 from Chapter 5, "Callus and Suspension Culture Induction,
Maintenance, and Characterization," by Alejandrina Robledo-Paz, María Nélida Vázquez-Sánchez, Rosa
María Adame-Alvarez, and Alba Estela Jofre-Garfias.
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Printed in the United States of America. 10 9 8 7 6 5 4 3 2 1
eISBN: 1-59259-959-1
ISSN: 1064-3745
Library of Congress Cataloging-in-Publication Data
Plant cell culture / edited by Victor M. Loyola-Vargas and Felipe
Vázquez-Flota. – 2nd. ed.
p. cm. – (Methods in molecular biology)
Includes bibliographical references (p. ).
ISBN 1-58829-547-8 (alk. paper)
1. Plant cell culture–Laboratory manuals. 2. Plant tissue culture–Laboratory manuals.
I. Loyola-Vargas, Victor M. II. Vázquez-Flota, Felipe. III. Methods in molecular biology
(Clifton, N.J.)
QK725.P5535 2005
571.6'382–dc22
2005046025
Preface
Cell culture methodologies have become standard procedures in most plant
laboratories. Full courses on the subject are taught as part of the curricula of
several programs in biological and agronomical sciences. However, the
apparent simplicity of cell culture technology should not lead to mistakenly
considering it as trivial. It would be very difficult to understand modern plant
biotechnology without in vitro cell cultures, and even today, the application of
recombinant DNA technology to important crops, such as peppers, is
hampered by the poor embryogenic or morphogenetic response of in vitro
cultured tissues.
This second edition of Plant Cell Culture Protocols follows a similar plot as
its predecessor. It also pursues similar goals; that is, to provide an updated
step-by-step guide to the most common and applicable techniques and
methods for plant tissue and cell culture. A total of 30 chapters, divided into
six sections, have been included. Topics selected cover from general
methodologies, such as culture induction, growth and viability evaluation, and
contamination control, to such highly specialized techniques as chloroplast
transformation, passing through the laborious process of protoplast isolation
and culture. Most of the protocols are currently used in the research programs
of the authors, or represent important parts of business projects aimed at the
generation of improved plant materials. Two appendixes have also been
included; the first discusses common principles for the formulation of culture
media and also lists the composition of the eight most commonly used media
formulations. The second appendix compiles a list of useful internet sites for
cell culture scientists. A total of more than 100 sites have been selected, based
on the quality of the information offered in them, as well as on their user friend-
liness. We anticipate that some these sites will be included among the reader’s
favorites (if they aren’t already).
We sincerely hope that readers will find this new edition of Plant Cell
Culture Protocols, which resides in the highly useful Methods in Molecular
Biology series, to be a major resource of information for their research projects;
this has been its real purpose.
We would like to thank the authors of each chapter for responding to our
constant requests for the materials, despite their reckless work schedules. They
made it possible for us to carry this job to its completion.
v
vi Preface
Finally, we should express our profound gratitude to Professor John Walker
for his trust in our experience to complete this project. We have certainly
enjoyed the opportunity to interact with colleagues from all over the world.
Victor M. Loyola-Varga
Felipe Vázquez-Flota
Contents
Preface ..............................................................................................................v
Contributors .....................................................................................................xi
PART I. INTRODUCTION
1 An Introduction to Plant Cell Culture: Back to the Future
Victor M. Loyola-Vargas and Felipe Vázquez-Flota.............................3
2 History of Plant Tissue Culture
Trevor A. Thorpe...................................................................................9
PART II. CELL CULTURE AND PLANT REGENERATION: THE FUNDAMENTALS
3 Pathogen and Biological Contamination Management:
The Road Ahead
Alan C. Cassells and Barbara M. Doyle..............................................35
4 Growth Measurements: Estimation of Cell Division
and Cell Expansion
Gregorio Godoy-Hernández and Felipe A. Vázquez-Flota................51
5 Callus and Suspension Culture Induction, Maintenance,
and Characterization
Alejandrina Robledo-Paz, María Nélida
Vázquez-Sánchez, Rosa María Adame-Alvarez,
and Alba Estela Jofre-Garfias.........................................................59
6 Measurement of Cell Viability in In Vitro Cultures
Lizbeth A. Castro-Concha, Rosa María Escobedo,
and María de Lourdes Miranda-Ham.............................................71
7 Cryopreservation of Embryogenic Cell Suspensions
by Encapsulation-Vitrification
Qiaochun Wang and Avihai Perl........................................................77
8 Somatic Embryogenesis in Picea Suspension Cultures
Claudio Stasolla..................................................................................87
9 Indirect Somatic Embryogenesis in Cassava for Genetic
Modification Purposes
Krit Raemakers, Isolde Pereira, Herma Koehorst van Putten,
and Richard Visser........................................................................101
vii
viii Contents
10 Direct Somatic Embryogenesis in Coffea canephora
Francisco Quiroz-Figueroa, Miriam Monforte-González,
Rosa M. Galaz-Ávalos, and Victor M. Loyola-Vargas..................111
PART III. PLANT PROPAGATION IN VITRO
11 A New Temporary Immersion Bioreactor System
for Micropropagation
Manuel L. Robert, José Luis Herrera-Herrera,
Gastón Herrera-Herrera, Miguel Ángel Herrera-Alamillo,
and Pedro Fuentes-Carrillo...........................................................121
12 Protocol to Achieve Photoautotrophic Coconut Plants
Cultured In Vitro With Improved Performance Ex Vitro
Gabriela Fuentes, Carlos Talavera, Yves Desjardins,
and Jorge M. Santamaría..............................................................131
13 Use of Statistics in Plant Biotechnology
Michael E. Compton.........................................................................145
14 An Efficient Method for the Micropropagation of Agave Species
Manuel L. Robert, José Luis Herrera-Herrera, Eduardo Castillo,
Gabriel Ojeda, and Miguel Angel Herrera-Alamillo....................165
15 Micropropagation of Endangered Plant Species
Zhihua Liao, Min Chen, Xiaofen Sun, and Kexuan Tang..................179
16 Clonal Propagation of Softwoods
Trevor A. Thorpe, Indra S. Harry, and Edward C. Yeung.................187
PART IV. APPLICATIONS FOR PLANT PROTOPLASTS
17 Isolation, Culture, and Plant Regeneration From Leaf
Protoplasts of Passiflora
Michael R. Davey, Paul Anthony, J. Brian Power,
and Kenneth C. Lowe....................................................................201
18 Isolation, Culture, and Plant Regeneration From Echinacea
purpurea Protoplasts
Zeng-guang Pan, Chun-zhao Liu, Susan J. Murch,
and Praveen K. Saxena.................................................................211
19 Production of Cybrids in Brassicaceae Species
Maksym Vasylenko, Olga Ovcharenko, Yuri Gleba,
and Nikolay Kuchuk.....................................................................219
20 Guard Cell Protoplasts: Isolation, Culture,
and Regeneration of Plants
Gary Tallman....................................................................................233
Contents ix
21 Production of Interspecific Hybrid Plants in Primula
Juntaro Kato and Masahiro Mii.........................................................253
PART V. PROTOCOLS FOR GENOMIC MANIPULATION
22 Agrobacterium-Mediated Transformation of Petunia Leaf Discs
Ingrid M. van der Meer.....................................................................265
23 Transformation of Wheat Via Particle Bombardment
Indra K. Vasil and Vilma Vasil..........................................................273
24 Chloroplast Transformation
Xiao-Mei Lu, Wei-Bo Yin, and Zan-Min Hu.....................................285
25 The Biochemical Basis for the Resistance to Aluminum
and Their Potential as Selection Markers
César De los Santos-Briones and Teresa Hernández-Sotomayor.............305
26 Transformation of Maize Via Agrobacterium tumefaciens
Using a Binary Co-Integrate Vector System
Zuo-yu Zhao and Jerry Ranch...........................................................315
PART VI. ACCUMULATION OF METABOLITES IN PLANT CELLS
27 Capsaicin Accumulation in Capsicum spp. Suspension Cultures
Neftalí Ochoa-Alejo..........................................................................327
28 Isolation and Purification of Ribosome-Inactivating Proteins
Sang-Wook Park, Balakrishnan Prithiviraj, Ramarao
Vepachedu, and Jorge M. Vivanco...............................................335
29 Catharanthus roseus Shoot Cultures for the Production
of Monoterpenoid Indole Alkaloids
Elizabeta Hernández-Domínguez, Freddy Campos-Tamayo,
Mildred Carrillo-Pech, and Felipe Vázquez-Flota........................349
30 Methods for Regeneration and Transformation in Eschscholzia
californica: A Model Plant to Investigate Alkaloid Biosynthesis
Benjamin P. MacLeod and Peter J. Facchini.....................................357
Appendix A: The Components of the Culture Media................................... 369
Appendix B: Plant Biotechnology and Tissue Culture
Resources on the Internet.............................................................. 379
Index ............................................................................................................385