Oxidants and Antioxidants Part B

574 Pages·1999·12.1 MB·English

by  Lester Packer (Eds.)

METHODS IN ENZYMOLOGY
EDITORS-IN-CHIEF
John N. Abelson Melvin I. Simon
DIVISION OF BIOLOGY
CALIFORNIA INSTITUTE OF TECHNOLOGY
PASADENA, CALIFORNIA
FOUNDING EDITORS
Sidney P. Colowick and Nathan 0. Kaplan
Methods in Enzymology
Volume 300
Oxidants and Antioxidants
Part B
EDITED BY
Lester Packer
UNIVERSITY OF CALIFORNIA
BERKELEY, CALIFORNIA
Editorial Advisory Board
Bruce Ames
Enrique Cadenas
Balz Frei
Matthew Grisham
Barry Halliwell
William Pryor
Catherine Rice-Evans
Helmut Sies
A P
0
ACADEMIC PRESS
San Diego New York Boston London Sydney Tokyo Toronto
Contributors to Volume 300
Article numbers are in parentheses following the names of contributors.
Affiliations listed are current.
IAN N. ACWORTH (31), Department of -rahP NEHPETS BRAND (35), ProScript, Inc., Cam-
macology, sttesuhcassaM College of Phar- ,egdirb sttesuhcassaM 93120
macy, Boston, sttesuhcassaM 51120 ANA MARIA OLIVEIRA BRETT (32), De-
BHARAT B. AGGARWAL (34), Cytokine -eR partamento ed Quimica, edadisrevinU De
hcraes Section, University of Texas .M .D ,arbmioC Coimbra ,9403 Portugal
Anderson Cancer ,retneC Houston, Texas I. HENDRIKJE BUss (13), Department of -aP
03077 thology, hcruhctsirhC School of ,enicideM
,hcruhctsirhC New Zealand
BRUCE N. AMES (10, 18), Department of Mo-
ralucel and Cell Biology, ytisrevinU of -ilaC NEHPETS E. BUXSER (28), Discovery -lonhceT
fornia, Berkeley, Berkeley, ainrofilaC 02749 ,seigo Pharmacia and pU john, Kalamazoo,
Michigan 10094
ELIAS S. J. ARN~R (24), Medical Nobel Insti-
etut for ,yrtsimehcoiB Department of -ideM Ho ZOON CHAE (23), Department of ,ygoloiB
lac Biochemistry and Biophysics, Karolin- egelloC of Sciences, Chonnam National
ska Institute, S-17177 Stockholm, Sweden ,ytisrevinU ,ugnawK Korea
M. FLINT BEAL (31), Department of Neurol- DANIEL F. CHURCH (9), Department of
Chemistry, Louisiana State ,ytisrevinU
ogy, sttesuhcassaM General Hospital, Bos-
Baton Rouge, Louisiana 30807
ton, sttesuhcassaM 41120
ELAINE M. CONNER (35), Department of Mo-
KENNETH B. BECKMAN (18), Department of
ralucel dna Cellular Physiology, Louisiana
Molecular and Cell Biology, University of
State University Medical Center, Shreve-
,ainrofilaC Berkeley, Berkeley, ainrofilaC
port, Louisiana 03117
02749
JAN R. CROWLEY (12, 16), Department of
ELIE BEIT-YANNAI (30), Department of -rahP ,enicideM Washington ytisrevinU School of
macology, School of Pharmacy, The -eH ,enicideM .tS Louis, Missouri 01136
brew ytisrevinU of ,melasureJ ,melasureJ -sI
MARIA L. CRUZ (32), Departamento ed -raF
lear 02119
m6cia, Faculdade ed Cidncias ,sacid~M
BARaARA S. BERLEXT (25), Laboratory of aicifitnoP edadisrevinU ,aci16taC Campinas
Biochemistry, National Heart, Lung, and ,409-02031 Brasil
Blood Institute, National Institutes of
T. DE LEO (26), Dipartimento id Fisio-
,htlaeH Bethesda, Maryland 0230-29802
logia Generale de Ambientale, Univer-
ELLIOT M. BERRY (30), Department of Hu- sitd id Napoli "Federico II," 43108-1 -aN
man Nutrition and Metabolism, ehT -eH poli, Italy
brew ytisrevinU of ,melasureJ ,melasureJ -sI ANTONIO DESTREE (35), ,tpircSorP Inc., Cam-
rael 02119 ,egdirb sttesuhcassaM 93120
NEIL V. BLOUGH (22), Department of -simehC Rul M. B. Digs (21), Departamento ed
yrt and Biochemistry, University of Mary- ,acimiuQ Instituto ocig6lonceT e ,raelcuN
,dnal College Park, Maryland 24702 5862-P ,mdvacaS Portugal
MIKHAIL B. BOGDANOV (31), Department of S. DI MEO (26), Dipartimento di aigoloisiF
Neurology, sttesuhcassaM General Hospi- elareneG de Ambientale, tgtisrevinU id Na-
,t,at Boston, sttesuhcassaM 41120 poll ociredeF'" ,II "' 43108-1 Napoli, Italy
xi
xii CONTRIBUTORS TO VOLUME 300
YMMAT R. DUGAS (9), Department of Chem- IVANO G. R. GUTZ (32), Instituto de Quimica,
istry, Louisiana State University, Baton Universidade De Sao Paulo, Sao Paulo
Rouge, Louisiana 30807 ,079-99550 Brasil
PETER ELLIOTr (35), ProScript, Inc., Cam- BARRY HALLIWELL (17), Pharmacology
bridge, Massachusetts 93120 Group, King's College, University of Lon-
PATRICIA EVANS (17), Neurodegenerative don, London SW3 6LX, United Kingdom
Disease Research Centre, King's College, GARRY J. HANDELMAN (6), Antioxidant Re-
University of London, London SW3 6LX, search Laboratory, U.S. Department of
United Kingdom Agriculture-Human Nutrition Research
DAVID FANBERSTEIN (43), Department of Center on Aging, Tufts University, Boston,
Pharmaceutics, School of Pharmacy, The Massachusetts 11120
Hebrew University o f Jerusalem, Jerusalem, DAVID R. HATHAWAY (45), Bristol-Myers
Israel 02119 Squibb Company, Princeton, New Jersey
JOHN L. FARBER (11), Department of Pathol- 0004-34580
ogy, Thomas Jefferson University, Philadel- ALFRED HAUSLADEN (38), Department of
phia, Pennsylvania 19107 Medicine, Duke University Medical ,retneC
SHARON FARFOURI (43), Department of Phar- Durham, North Carolina 27710
maceutics, School of Pharmacy, The He- YELNATS L. HAZEN (12, 16), Department of
brew University of Jerusalem, Jerusalem, Is- Cell Biology, Cleveland Clinic Foundation,
rael 02119 Cleveland, Ohio 59144
BALZ FREI (3, 7), Linus Pauling Institute, Ore- JAY W. HEINECKE (12, 16), Departments of
gon State University, Corvallis, Oregon Medicine, Molecular Biology and Pharma-
13379 cology, Washington University School of
JOSEPH P. GAUT (12), Department of Medi- Medicine, .tS Louis, Missouri 01136
cine, Washington University School of Med- HAROLD J. HELBOCK (10, 18), Department of
icine, .tS Louis, Missouri 01136 Molecular and Cell Biology, University of
PETER G. GEIGER (4), Department of Bio- California, Berkeley, Berkeley, California
chemistry, Medical College of Wisconsin, 02749
Milwaukee, Wisconsin 53226 MIDORI HIRAMATSU (27), Institute for Life
ALBERT W. GmOTTI (4), Department of Bio- Support Technology, Yamagata Technopo-
chemistry, Medical College of Wisconsin, lis Foundation, Yamagata ,3742-099 Japan
Milwaukee, Wisconsin 53226 ARNE HOLMOREN (24), Medical Nobel Insti-
PETER A. GLASCOTT, JR. (11), RhOne-Poulenc tute for Biochemistry, Department of Medi-
Rorer Research and Development, Drug cal Biochemistry and Biophysics, Karolin-
Safety, Collegeville, Pennsylvania 19426- ski Institute, S-17177 Stockholm, Sweden
4990 UDO HOPPE (42), Paul Gerson Unna-Skin
KISHORCHANDRA GOHIL (40), Membrane Research Center, D-20245 Hamburg,
Bioenergetics Group, Department of Mo- Germany
lecular and Cell Biology, University of FONG F. Hsu (12, 16), Department of Medi-
California, Berkeley, Berkeley, Califor- cine, Washington University School of Med-
nia 0023-02749 icine, .tS Louis, Missouri 01136
MATTHEW B. GRISHAM (35), Department of YVONNE M. W. JANSSEN (36), Department of
Molecular and Cellular Physiology, Louisi- Pathology, University of Vermont, Bur-
ana State University Medical Center, lington, Vermont 50450
Shreveport, Louisiana 2393-03117 SANO WON KANO (23), Laboratory of Cell
PETER L. GUTIERREZ (22), Greenbaum Can- ,gnilangiS National Heart, Lung, and Blood
cer Center, University of Maryland School Institute, National Institutes of Health,
of Medicine, Baltimore, Maryland 10212 Bethesda, Maryland 29802
CONTRIBUTORS TO VOLUME 300 xiii
NHOJ F. ,YENAEK .RJ (3, 7), Evans Memorial SEMAJ K. OAIL (37), Cardiovascular Division,
Department of Medicine and Whitaker Car- Department of Medicine, Brigham and
diovascular Institute, Boston University Women's Hospital, Boston, Massachusetts
School of Medicine, Boston, Massachu- 51120
setts 81120 GNAKNAIJ UIL (10), Department of Molecular
YNOHTNA J. ELTTEK (14), Free Radical Re- and Cell Biology, University of California,
search Group, Department of Pathology, Berkeley, Berkeley, California 02749
Christchurch School of Medicine, Christ- NEFFETS LOFF (19), Institute of Public Health,
church, New Zealand University of Copenhagen, DK-2200 -oC
NOR NEHOK (30, 43), Department of Pharma- penhagen, Denmark
ceutics, School of Pharmacy, The Hebrew SADINOEL SARYL (17), Neurodegenerative
University of Jerusalem, Jerusalem, Israel Disease Research Centre, King's College,
02119 University of London, London SW3 6LX,
OKIKAM USTAMOK (27), Institute for Life Sup- United Kingdom
port Technology, Yamagata Technopolis EDLITAM ONIROIAM (5), Department of Bio-
Foundation, Yamagata ,3742-099 Japan logical Chemistry, University of Padova,
DLOTIW IKSWOTYROK (4), Department of 12153-1 Padova, Italy
Biochemistry, Medical College of Wiscon- SALGUOD R. MCCABE (31), ESA, Inc.,
sin, Milwaukee, Wisconsin 62235 Chelmsford, Massachusetts 42810
KOHSA RAMUK (34), Cytokine Research Sec- WEI GNIP IEM (42), Paul Gerson Unna-Skin
tion, University of Texas, M. D. Anderson Research Center, D-20245 Hamburg,
Cancer Center, Houston, Texas 03077 Germany
EGIL MAVK (33), Department of Pharmacy LEAHCIM O. REGNEM (46), Institute for Clini-
and Pharmacology, University of Bath, cal and Experimental Surgery, Univer-
Bath, Ba2 7AY, United Kingdom sity of Saarland, D-66421 Homburg/Saar,
Germany
ORUAM A. AELACS-AL (32), lnstituto de
ELIME R. RELHOM III (45), University of
Quimica, Universidade De DaS Paulo, DaS
Pennsylvania School of Medicine, Philadel-
Paulo ,079-99550 Brasil
phia, Pennsylvania 40191
NAAITSIRHC HGRUBNEWUEEL (16), Depart-
NOSAJ D. WORROM (1), Departments of Medi-
ment of Medicine, Washington University
cine and Pharmacology, Vanderbilt Univer-
School of Medicine, .tS Louis, Missouri
sity School of Medicine, Nashville, Tennes-
01136
see 23273
NOTNA-SNAH RHEL (46), Institute of Pathol-
BOKCAJ ZTlVOKSOM (25), Laboratory of -DiB
ogy, Johannes Gutenberg University,
chemistry, National Heart, Lung, and Blood
10155-D Mainz, Germany
Institute, National Institutes of Health,
YENDOR L. ENIVEL (15), Laboratory of -DiB Bethesda, Maryland 0230-29802
chemistry, National Heart, Lung, and Blood
IMO-ORIH IRWOM (3), Evans Memorial De-
btstitute, National Institutes of Health,
partment of Medicine and Whitaker Cardio-
Bethesda, Maryland 29802
vascular Institute, Boston University School
NURDUG LEWlN (44), Research Institute of Medicine, Boston, Massachusetts 81120
for Antioxidant Therapy, 10559 Berlin,
ENNAID M. RELLEUM (16), Department of
Germany
Medicine, Washington University School of
ARO~rAS A. HCSlWEL (15), AtheroGenics, Inc., Medicine, .tS Louis, Missouri 01136
Norcross, Georgia 17002 JAFFAR HEDAZ-ZOORUON (2, 8), Division of
BEmEI LI (22), Department of Chemistry and Clinical Pharmacology and Toxicology,
Biochemistry, University of Maryland, Col- Department of Medicine, University College
lege Park, Maryland 24702 London, London WIN 8AA, England
xiv CONTRIBUTORS TO VOLUME 300
RETSEL REKCAP (39--41), Membrane -reneoiB raluc ,etutitsnI Boston ytisrevinU School of
sciteg Group, Department of raluceloM and ,enicideM Boston, sttesuhcassaM 81120
Cell Biology, University of ,ainrofilaC DRAHREG NNAMREUAS (42), Paul Gerson
,yelekreB Berkeley, California 0023-02749 Unna-Skin Research Center, D-20245
VITO J. ALLEBMOLAP (35), ProScript, Inc., Hamburg, Germany
,egdirbmaC sttesuhcassaM 93120 GERI ADAWAS (28), lacituecamrahP Develop-
OINOTNA M. ONIROTSAP (5), emoclleW-oxalG ment ,I Pharmacia dna Upjohn, Kalama-
S.p.A. Medicine's Research Centre, -eV zoo, Michigan 10094
,anor Italy NADNAHC K. NES (36, 39, 40), Membrane
ENITSIRHC PIEN (35), ProScript, Inc., Cam- Bioenergetics Group, Department of
,egdirb sttesuhcassaM 93120 Molecular and Cell Biology, University of
,ainrofilaC Berkeley, Berkeley, ainrofilaC
SONAILYTS M. SIKAREPIP (20), DNA Repair
,0023-02749 and Department of ,ygoloisyhP
Laboratory, Institute of Biology, NCSR
ytisrevinU of Kuopio, Finland
"Demokritos," Athens, eceerG
AIVLIS H. P. ONARRES (32), Instituto ed Quim-
I~OR PoPov (44), hcraeseR Institute for Anti-
,aci edadisrevinU De oaS Paulo, oaS Paulo
oxidant Therapy, 10559 Berlin, Germany
,079-99550 Brasil
J. LEAHCIM NOTSOP (25), Laboratory of Bio-
NNELG T. YREAWHS (3, 7), Evans Memorial
,yrtsimehc National ,traeH Lung, and Blood
Department of enicideM and Whitaker -raC
Institute, National Institutes of Health,
diovascular Institute, Boston University
,adsehteB Maryland 0230-29802
School of Medicine, Boston, Massachu-
KIRNEH NESUHGNE NESLUOP (19), Depart- sttes 81120
ment of Clinical Pharmacology, Rigshos-
NITRAM REKCEIPS (37), ralucsavoidraC Divi-
,telatip ytisrevinU of ,negahnepoC D 0022-K
,nois Department of ,enicideM Brigham and
,negahnepoC Denmark
Women's Hospital, Boston, sttesuhcassaM
JANE E. NESSUMSAR (16), Department of 51120
,enicideM Washington ytisrevinU School of
ZNARF B,g,TS (42), Paul Gerson Unna-Skin
,enicideM .tS Louis, Missouri 01136
Research Center, D-20245 Hamburg,
SAMOHT J. RAUB (28), lacituecamrahP -leveD ynamreG
opment ,I Pharmacia and pU john, -amalaK
EARL R. NAMTDATS (25), Laboratory of Bio-
zoo, Michigan 10094
,yrtsimehc National ,traeH Lung, and Blood
EUS Goo RHEE (23), Laboratory of Cell -giS Institute, National Institutes of Health,
,gnilan National Heart, Lung, and Blood ,adsehteB Maryland 0230-29802
Institute, National Institutes of Health, NAHTANOJ .S RELMATS (38), Howard Hughes
,adsehteB Maryland 29802 lacideM ,etutitsnI Duke ytisrevinU lacideM
L. NOSKCAJ STREBOR II (1), Departments of ,retneC Durham, North aniloraC 01772
Pharmacology and Medicine, Vanderbilt A1SAPSA M. UOISSAT (20), Institute of Biol-
ytisrevinU School of ,enicideM ,ellivhsaN ,ygo NCSR "Demokritos," Athens, eceerG
eessenneT 23273
Jo.~o P. TELO (21), Secfao ed Qu(mica -nagrO
ITAWHSAS YOR (39, 40), Membrane -reneoiB ,aci Instituto Superior ,ocincdT P-1096 Lis-
sciteg Group, Department of raluceloM and aob codex, Portugal
Cell Biology, University of ,ainrofilaC
SNEJ J. THIELE (41), Institut far Physiolo-
,yelekreB Berkeley, California 0023-02749
ehcsig Chemie ,I -isrevinU-enieH-hcirnieH
NAEETS RYTER (33), Department of Internal ,tat D-40225 Dasseldorf Germany
,enicideM Southern Illinois School of -ideM NERO HSORIT (30, 43), Department of Phar-
,enic ,dleifgnirpS Illinois 20726 ,scituecam School of Pharmacy, ehT He-
NOSAJ M. IIMAS (7), Evans Memorial -trapeD brew ytisrevinU of ,melasureJ ,melasureJ -sI
ment of enicideM and Whitaker -savoidraC rael 02119
CONTRIBUTORS TO VOLUME 300 XV
JOHN TURK (16), Department of Medicine, CHRISTINE C. WINTERBOURN (13), Depart-
Washington University School of Medicine, ment of Pathology, Christchurch School of
.tS Louis, Missouri 01136 Medicine, Christchurch, New Zealand
REX M. TYRRELL (33), Department of Phar- HENRY WONG (35), ProScript, Inc., Cam-
macy and Pharmacology, University of bridge, Massachusetts 93120
Bath, Bath, BA2 7A Y, United Kingdom HELEN C. YEO (10), Roche Bioscience, Palo
FuLvIO URSINI (5), Department of Biological Alto, California 7931-40349
Chemistry, University of Padova, 12153-1 HIDEKATSU YOKOYAMA (29), Institute for
Padova, Italy Life Support Technology, Yamagata Tech-
PETER VAJKOCZY (46), Neurosurgical Depart- nopolis Foundation, Yarnagata ,3742-099
ment, Klinikurn Mannheim, University of Japan
Heidelberg, D-68167 Mannheim, Germany ADRIANA ZAMBURLINI (5), Department of Bi-
P. VENDITTI (26), Dipartimento di Fisiologia ological Chemistry, University of Padova,
Generale ed Ambientale, tgtisrevinU di Na- 12153-1 Padova, Italy
poli "Federico II, "' 43108-1 Napoli, Italy ABRAHAM ZELKOWICZ (43), Department of
ABEL J. S. C. VIEIRA (21), Secgao de Quimica ,scituecanrrahP School of Pharmacy, The
Org~nica, Instituto Superior T~cnico, Hebrew University of Jerusalem, Jerusalem,
P-1096 Lisboa codex, Portugal Israel 02119
EVANGELOS-E. V1SVARDIS (20), Institute of Lucio ZENNARO (5), Department of Bio-
Biology, NCSR "Demokritos," Athens, logical Chemistry, University of Padova,
Greece 12153-1 Padova, Italy
BRI()ITTE VOLLMAR (46), Institute for Clini- IEWGNA1L ZHONG (24), Medical Nobel Insti-
cal and Experimental Surgery, Univer- tute for Biochemistry, Department of Medi-
sity of Saarland, D-66421 Homburg/Saar, cal Biochemistry and Biophysics, Karolin-
Germany ska Institute, S-17177 Stockholm, Sweden
Preface
The importance of reactive oxygen and nitrogen species (ROS and
RNS) and antioxidants in health and disease has now been recognized in
all of the biological sciences and has assumed special importance in the
biomedical sciences. Overwhelming evidence indicates that ROS play a
role in most major health problems, that antioxidants play a critical role
in wellness and health maintenance, and that by inhibiting oxidative damage
to molecules, cells, and tissues prevent chronic and degenerative diseases.
We now know that ROS are essential for many enzyme-catalyzed re-
actions. Low levels of reactive oxygen and reactive nitrogen species are
signaling molecules. At high concentration, these ROS are essential in
the antitumor, antimicrobial, antiparasitic action, etc., of neutrophils and
macrophages and contribute to oxidative damage to molecules, cells, and
tissues.
In this volume we have updated the assays and methods applicable to
assaying oxidative damage to biomolecules, particularly macromolecules
and assays that are useful for evaluating activities in cells and tissues. New
methods are swiftly evolving for assaying the oxidant- and antioxidant-
redox-sensitive steps in cell signaling and gene expression. And, finally, the
development of new technologies is allowing for the noninvasive evaluation
of oxidant and antioxidant action. These developments and methodologies
can be expected in the future to be applicable to clinical situations in which
the early detection of disease remains a problem of paramount importance.
In bringing this volume to fruition, credit must be given to experts in
various specialized fields of oxidant and antioxidant research. Our apprecia-
tion is to the contributors who, with those who helped select them, have
produced this state-of-the-art volume on oxidant and antioxidant methodol-
ogy. The topics included were chosen on the excellent advice of Bruce N.
Ames, Enrique Cadenas, Balz Frei, Matthew Grisham, Barry HaUiwell,
William Pryor, Catherine Rice-Evans, and Helmut Sies. To these col-
leagues, I extend my sincere thanks and most grateful appreciation.
LESTER PACKER
iivx
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
EMULOV II. Preparation and Assay of Enzymes
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
EMULOV III. Preparation and Assay of Substrates
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
EMULOV IV. Special Techniques for the Enzymologist
Edited by YENDIS P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV V. Preparation and Assay of Enzymes
Edited by YENDIS P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV VI. Preparation and Assay of Enzymes (Continued)
Preparation and Assay of Substrates
Special Techniques
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
EMULOV VII. Cumulative Subject Index
Edited by SIDNEY P, COLOWICK AND NATHAN O. KAPLAN
EMULOV VIII. Complex Carbohydrates
Edited by ELIZABETH F. NEUFELD AND VICTOR GINSBURG
EMULOV IX. Carbohydrate Metabolism
Edited by SILLIW A. DOOW
EMULOV X. Oxidation and Phosphorylation
Edited by DLANOR W. KOORBATSE DNA DRANYAM E. NAMLLUP
EMULOV XI. Enzyme Structure
Edited by C. H. W. SRIH
EMULOV XII. Nucleic Acids (Parts A and B)
Edited by LAWRENCE GROSSMAN AND KIVIE MOLDAVE
EMULOV XIII. Citric Acid Cycle
Edited by J. .M NIETSNEWOL
EMULOV XIV. Lipids
Edited by J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited by DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
Edited by HTENNEK NITSUK
xix
XX METHODS IN ENZYMOLOGY
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
by Edited TREBREH ROBAT DNA CELIA ETIHW ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited yb DLANOD B. KCIMRoCcM DNA LEUMEL D. THGIRW
EMULOV XIX. Proteolytic Enzymes
Edited by EDURTREG E. NNAMLREP DNA OLZSAL DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
by Edited KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
by Edited MAILLIW B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited by NAS YNOHTNA ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited yb YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited by .C H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by .C H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited yb .C H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
by Edited ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX, Nucleic Acids and Protein Synthesis (Part F)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXII. Biomembranes (Part B)
Edited yb YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by AHTRAM G. SINNED DNA DRAWDE A. SINNED
EMULOV XXXlV. Affinity Techniques (Enzyme Purification: Part B)
Edited by MAILLIW B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XXXV. Lipids (Part B)
Edited by NHOJ .M NIETSNEWOL