Table Of ContentMETHODS IN ENZYMOLOGY
EDITORS-IN-CHIEF
John N. Abelson Melvin I. Simon
DIVISIONOFBIOLOGY
CALIFORNIAINSTITUTEOFTECHNOLOGY
PASADENA,CALIFORNIA
FOUNDINGEDITORS
Sidney P. Colowick and Nathan O. Kaplan
Contributors to Volume 394
Articlenumbersareinparenthesesandfollowingthenamesofcontributors.
Affiliationslistedarecurrent.
Thomas B. Acton (8), Center for Ad- Paul B. Card (1), Departments of Bio-
vanced Biotechnology and Medicine, chemistryandPharmacology,University
Department of Molecular Biology and of Texas Southwestern Medical Center,
Biochemistry, Rutgers University, Pis- Dallas,Texas75390
cataway,NewJersey08854
Yi-WenChiang (8), Centerfor Advanced
James Aramini (8), Center for Advanced Biotechnology and Medicine, Depart-
Biotechnology and Medicine, Depart- ment of Molecular Biology and Bioche-
ment of Molecular Biology and Bio- mistry, Rutgers University, Piscataway,
chemistry, Rutgers University, NewJersey08854
Piscataway,NewJersey08854
TeresaCliment(8),CenterforAdvanced
Cheryl Arrowsmith (5), Ontario Cancer Biotechnology and Medicine, Depart-
Institute and Department of Medical ment of Molecular Biology and Bioche-
Biophysics, University of Toronto, mistry, Rutgers University, Piscataway,
Toronto,Ontario,M5G2M9Canada NewJersey08854
HanudattaS.Atreya(4),Departmentof Bonnie Cooper (8), Center for Advanced
Chemistry, University at Buffalo, The Biotechnology and Medicine, Depart-
State University of New York, Buffalo, ment of Molecular Biology and Bioche-
NewYork14260 mistry, Rutgers University, Piscataway,
NewJersey08854
Michael C. Baran (5, 8), Center for Ad-
vanced Biotechnology and Medicine, LorenzoCorsini(2),InstituteforBiophy-
Department of Molecular Biology and sicalChemistry,UniversityofFrankfurt,
Biochemistry, Rutgers University, 60439Frankfurt,Germany
Piscataway,NewJersey08854
L. David Finger (22), Department of
AdBax(3),NationalInstituteofDiabetes Chemistry and Biochemistry, University
and Digestive and Kidney Diseases, of California, Los Angeles, California
National Institutes of Health, Bethesda, 90095
Maryland20892
A. A. De Angelis (14), Department of
G. C. Benison (7), Department of Bio- ChemistryandBiochemistry,University
chemistry and Molecular Biology, Uni- of California, San Diego, La Jolla,
versity of Georgia, Athens, Georgia California92093
30602
FrankDelaglio(3),NationalInstituteof
P. Bernado´ (17), Institut de Biologie DiabetesandDigestiveandKidneyDis-
Structurale, Jean Pierre Ebel, 38027 eases, National Institutes of Health,
Grenoble,France Bethesda,Maryland20892
ix
x contributors to volume 394
NataliaG.Denissova(8),CenterforAd- Kevin H. Gardner (1), Departments of
vanced Biotechnology and Medicine, Biochemistry and Pharmacology, Uni-
Department of Molecular Biology and versity of Texas Southwestern Medical
Biochemistry, Rutgers University, Pis- Center,Dallas,Texas75390
cataway,NewJersey08854
MarkGerstein(8),DepartmentofMole-
Shawn M. Douglas (8), Department of cular Biophysics and Biochemistry
Molecular Biophysics and Biochemistry and Department of Computer Science,
and Department of Computer Science, Yale University, New Haven, Connecti-
YaleUniversity,NewHaven,Connecticut cut06520
06520
C.V.Grant(14),DepartmentofChemis-
Volker Do¨tsch (2), Institute for Biophy- try and Biochemistry, University of Ca-
sicalChemistry,UniversityofFrankfurt, lifornia,SanDiego,LaJolla,California
60439Frankfurt,Germany 92093
FlorianDurst(2),InstituteforBiophysi- MichaelJ.Grey(18),DepartmentofBio-
cal Chemistry, University of Frankfurt, chemistry and Molecular Biophysics,
60439Frankfurt,Germany Columbia University, New York,
H. Jane Dyson (11), Department of NewYork10032
Molecular Biology and Skaggs Institute AlexanderGrishaev(10),NationalInsti-
forChemicalBiology,ScrippsResearch tuteofDiabetesandDigestiveandKid-
Institute, La Jolla, California 92037 ney Diseases, National Institutes of
Christian Eichmu¨ller (6), Institute of Health,Bethesda,Maryland20892
Organic Chemistry, University of In- Kristin C. Gunsalus (8), Department of
nsbruck,A-6020Innsbruck,Austria Biology, Center for Comparative Func-
ElanZ.Eisenmesser(21),Departmentof tional Genomics, New York Univeristy,
NewYork,NewYork10003
Biochemistry, Brandeis University,
Waltham,Massachusetts02454 Philip J. Hajduk (23), Abbott Labora-
Charles D. Ellis (12), Department of tories,AbbottPark,Illinois60064
Biochemistry, Vanderbilt University Kathleen B. Hall (19), Department
School of Medicine, Nashville, Tennes-
of Biochemistry and Molecular
see37232
Biophysics, Washington University
JohnK.Everett(8),CenterforAdvanced School of Medicine, St. Louis,
Biotechnology and Medicine, Depart- Missouri 63110
ment of Molecular Biology and Bioche-
Chi Kent Ho (8), Center for Advanced
mistry, Rutgers University, Piscataway,
Biotechnology and Medicine, Depart-
NewJersey08854
ment of Molecular Biology and Bioche-
JuliFeigon(22),DepartmentofChemistry mistry, Rutgers University, Piscataway,
and Biochemistry, University of Califor- NewJersey08854
nia,LosAngeles,California90095
BerndHoffmann(6),InstituteofTheoreti-
J.Garcı´adelaTorre(17),Departamento cal Chemistry and Molecular Structural
deQu´ımicaF´ısica,UniversidaddeMur- Biology, University of Vienna, A-1030
cia,30071Murcia,Spain Vienna,Austria
contributors to volume394 xi
Yuanpeng Janet Huang (5), Center for Robert Konrat (6), Institute of Theoreti-
AdvancedBiotechnologyandMedicine, cal Chemistry and Molecular Structural
Department of Molecular Biology Biology, University of Vienna, A-1030
and Biochemistry, Rutgers University, Vienna,Austria
Piscataway,NewJersey08854
Georg Kontaxis (3), Department of
John F. Hunt (8), Department of Biolo- Theoretical Chemistry and Molecular
gical Sciences, Columbia University, Structural Biology, University of
New York, New York 10027 Vienna,A-1030Vienna,Austria
Jeffrey R. Huth (23), Abbott Labora- Miguel Llina´s (10), Department of
tories,AbbottPark,Illinois60064 Chemistry, Carnegie Mellon University,
Pittsburgh,Pennsylvania15213
Peter M. Hwang (13), Departments
of Medical Genetics, Biochemistry, PeterJ.Lukavsky(16),MRCLaboratory
and Chemistry, University of of Molecular Biology, Cambridge, CB2
Toronto, Toronto, Ontario, M5S 1A1 2QH,England
Canada
Li Chung Ma (8), Center for Advanced
MasayoriInouye(8),DepartmentofBio- Biotechnology and Medicine, Depart-
chemistry,RobertWoodJohnsonMedi- mentofMolecularBiologyandBiochem-
cal School, University of Medicine and istry, Rutgers University, Piscataway,
Dentistry of New Jersey, Piscataway, NewJersey08854
NewJersey08854 Daphne Macapagal (8), Center for Ad-
Jaison Jacob (12), Department of vanced Biotechnology and Medicine,
Department of Molecular Biology and
Biochemistry, Vanderbilt University
Biochemistry, Rutgers University, Pis-
School of Medicine, Nashville, Tennes-
cataway,NewJersey08854
see37232
Thomas L. James (24), Department of K.L.Mayer(7),DepartmentofBiochem-
istry and Molecular Biology, University
PharmaceuticalChemistry,Universityof
ofGeorgia,Athens,Georgia30602
California, San Francisco, California
94143 Moriz Mayer (24), Biovertis AG, 1080
Vienna,Austria
D.H.Jones(14),DepartmentofChemis-
try and Biochemistry, University of Ca- M.F.Mesleh(14), DepartmentofChem-
lifornia,SanDiego,LaJolla,California istryandBiochemistry,UniversityofCa-
92093 lifornia,SanDiego,LaJolla,California
92093
Murthy D. Karra (12), Department of
Biochemistry, Vanderbilt University GaetanoT.Montelione(5,8),Centerfor
School of Medicine, Nashville, Tennes- AdvancedBiotechnologyandMedicine,
see37232 Department of Molecular Biology and
Biochemistry, Rutgers University and
LewisE.Kay(13),DepartmentsofMedi- Robert Wood Johnson Medical School,
calGenetics,Biochemistry,andChemis- Piscataway,NewJersey08854
try, University of Toronto, Toronto,
Ontario,M5S1A1Canada Hunter N. B. Moseley (5), Center for
AdvancedBiotechnologyandMedicine,
Dorothee Kern (21), Department of Department of Molecular Biology
Biochemistry, Brandeis University, and Biochemistry, Rutgers University,
Waltham,Massachusetts02454 Piscataway,NewJersey08854
xii contributors to volume 394
S.J.Opella(14),DepartmentofChemis- Daryl R. Sauer (23), Abbott Labora-
try and Biochemistry, University of Ca- tories,AbbottPark,Illinois60064
lifornia,SanDiego,LaJolla,California
Zach Serber (2), Department of Molecu-
92093
lar Pharmacology, Stanford University
KirillOxenoid(12),DepartmentofBio- SchoolofMedicine,Stanford,California
chemistry, Vanderbilt University School 94305
ofMedicine,Nashville,Tennessee37232
Ritu Shastry (8), Center for Advanced
Arthur G. Palmer, III (18), Department Biotechnology and Medicine, Depart-
ofBiochemistryandMolecularBiophy- ment of Molecular Biology and Bioche-
sics, Columbia University, New York, mistry, Rutgers University, Piscataway,
NewYork10032 NewJersey08854
S.H.Park(14),DepartmentofChemistry Liang-yu Shih (8), Center for Advanced
andBiochemistry,UniversityofCalifor- Biotechnology and Medicine, Depart-
nia, San Diego, La Jolla, California ment of Molecular Biology and Bioche-
92093 mistry, Rutgers University, Piscataway,
M.Pons(17),DepartmentdeQu´ımicaOr- NewJersey08854
ga`nica, Universitat de Barcelona, 08028 Ichio Shimada (20), Division of Physical
Barcelona, Spain, and Laboratori de Chemistry, Graduate School of Phar-
RMN de Biomole`cules, Parc Cientific maceutical Sciences, The University
deBarcelona,08028Barcelona,Spain of Tokyo, Hongo, Tokyo 113-0033,
RobertPowers(5),DepartmentofChem- Japan
istry, University of Nebraska—Lincoln, Scott A. Showalter (19), Department
Lincoln,Nebraska68588
of Biochemistry and Molecular
J. H. Prestegard (7), Complex Carbohy- Biophysics, Washington University
School of Medicine, St. Louis, Mis-
drate Research Center, University of
souri 63110
Georgia,Athens,Georgia30602
Joseph D. Puglisi (16), Department of FrankSo¨nnichsen(12),ClevelandCenter
Structural Biology, Stanford University for Structural Biology and Department
SchoolofMedicine,Stanford,California of Physiology and Biophysics, Case
94305 Western Reserve University, Cleveland,
Ohio44106
Paranji K. Rajan (8), Center for Ad-
vanced Biotechnology and Medicine, Othmar Steinhauser (6), Institute of
Department of Molecular Biology and Theoretical Chemistry and Molecular
Biochemistry, Rutgers University, Pis- StructuralBiology,UniversityofVienna,
cataway,NewJersey08854 A-1030Vienna,Austria
Carol A. Rohl (9), Department of Bio- ChaohongSun(23),AbbottLaboratories,
molecular Engineering, University of AbbottPark,Illinois60064
California,SantaCruz,California95064
G.V.T. Swapna (8), Center for Advanced
CharlesR.Sanders(12),Departmentof Biotechnology and Medicine, Depart-
Biochemistry and Center for Structural ment of Molecular Biology and Bioche-
Biology,VanderbiltUniversitySchoolof mistry, Rutgers University, Piscataway,
Medicine,Nashville,Tennessee37232 NewJersey08854
contributors to volume394 xiii
Thomas Szyperski (4, 5), Department of MichaelWilson(8),DepartmentofMo-
Chemistry, University at Buffalo, The lecularBiophysicsandBiochemistryand
State University of New York, Buffalo, Department of Computer Science, Yale
NewYork14260 University, New Haven, Connecticut
06520
RobertoTejero(5),CenterforAdvanced
Peter E. Wright (11), Department
Biotechnology and Medicine, Depart-
of Molecular Biology and Skaggs Insti-
ment of Molecular Biology and
tute for Chemical Biology, Scripps Re-
Biochemistry, Rutgers University, Pis-
search Institute, La Jolla, California
cataway,NewJersey08854
92037
Changlin Tian (12), Department of Magnus Wolf-Watz (21), Department
Biochemistry, Vanderbilt University of Biochemistry, Brandeis University,
School of Medicine, Nashville, Tennes- Waltham,Massachusetts02454
see37232
Haihong Wu (22), Department of
H. Valafar (7), Department of ChemistryandBiochemistry,University
Computer Science, University of South of California, Los Angeles, California
Carolina, Columbia, South Carolina 90095
29208 Margaret Wu (8), Center for Advanced
Biotechnology and Medicine, De-
Chunyu Wang (18), Department of Bio-
partment of Molecular Biology and
chemistry and Molecular Biophysics,
Biochemistry, Rutgers University,
Columbia University, New York, New
Piscataway,NewJersey08854
York10032
Rong Xiao (8), Center for Advanced
Gerhard Wider (15), Institute for Biotechnology and Medicine, Depart-
Molecular Biology and Biophysics, ment of Molecular Biology and
ETH Zurich, CH-8093 Zurich, Biochemistry, Rutgers University,
Switzerland Piscataway, New Jersey 08854
METHODS IN ENZYMOLOGY
VolumeI.PreparationandAssayofEnzymes
EditedbySidneyP.ColowickandNathanO.Kaplan
VolumeII.PreparationandAssayofEnzymes
EditedbySidneyP.ColowickandNathanO.Kaplan
VolumeIII.PreparationandAssayofSubstrates
EditedbySidneyP.ColowickandNathanO.Kaplan
VolumeIV.SpecialTechniquesfortheEnzymologist
EditedbySidneyP.ColowickandNathanO.Kaplan
VolumeV.PreparationandAssayofEnzymes
EditedbySidneyP.ColowickandNathanO.Kaplan
VolumeVI.PreparationandAssayofEnzymes(Continued)
PreparationandAssayofSubstrates
SpecialTechniques
EditedbySidneyP.ColowickandNathanO.Kaplan
VolumeVII.CumulativeSubjectIndex
EditedbySidneyP.ColowickandNathanO.Kaplan
VolumeVIII.ComplexCarbohydrates
EditedbyElizabethF.NeufeldandVictorGinsburg
VolumeIX.CarbohydrateMetabolism
EditedbyWillisA.Wood
VolumeX.OxidationandPhosphorylation
EditedbyRonaldW.EstabrookandMaynardE.Pullman
VolumeXI.EnzymeStructure
EditedbyC.H.W.Hirs
VolumeXII.NucleicAcids(PartsAandB)
EditedbyLawrenceGrossmanandKivieMoldave
VolumeXIII.CitricAcidCycle
EditedbyJ.M.Lowenstein
VolumeXIV.Lipids
EditedbyJ.M.Lowenstein
VolumeXV.SteroidsandTerpenoids
EditedbyRaymondB.Clayton
xv
xvi methods in enzymology
VolumeXVI.FastReactions
EditedbyKennethKustin
VolumeXVII.MetabolismofAminoAcidsandAmines(PartsAandB)
EditedbyHerbertTaborandCeliaWhiteTabor
VolumeXVIII.VitaminsandCoenzymes(PartsA,B,andC)
EditedbyDonaldB.McCormickandLemuelD.Wright
VolumeXIX.ProteolyticEnzymes
EditedbyGertrudeE.PerlmannandLaszloLorand
VolumeXX.NucleicAcidsandProteinSynthesis(PartC)
EditedbyKivieMoldaveandLawrenceGrossman
VolumeXXI.NucleicAcids(PartD)
EditedbyLawrenceGrossmanandKivieMoldave
VolumeXXII.EnzymePurificationandRelatedTechniques
EditedbyWilliamB.Jakoby
VolumeXXIII.Photosynthesis(PartA)
EditedbyAnthonySanPietro
VolumeXXIV.PhotosynthesisandNitrogenFixation(PartB)
EditedbyAnthonySanPietro
VolumeXXV.EnzymeStructure(PartB)
EditedbyC.H.W.HirsandSergeN.Timasheff
VolumeXXVI.EnzymeStructure(PartC)
EditedbyC.H.W.HirsandSergeN.Timasheff
VolumeXXVII.EnzymeStructure(PartD)
EditedbyC.H.W.HirsandSergeN.Timasheff
VolumeXXVIII.ComplexCarbohydrates(PartB)
EditedbyVictorGinsburg
VolumeXXIX.NucleicAcidsandProteinSynthesis(PartE)
EditedbyLawrenceGrossmanandKivieMoldave
VolumeXXX.NucleicAcidsandProteinSynthesis(PartF)
EditedbyKivieMoldaveandLawrenceGrossman
VolumeXXXI.Biomembranes(PartA)
EditedbySidneyFleischerandLesterPacker
VolumeXXXII.Biomembranes(PartB)
EditedbySidneyFleischerandLesterPacker
VolumeXXXIII.CumulativeSubjectIndexVolumesI-XXX
EditedbyMarthaG.DennisandEdwardA.Dennis
VolumeXXXIV.AffinityTechniques(EnzymePurification:PartB)
EditedbyWilliamB.JakobyandMeirWilchek
methodsin enzymology xvii
VolumeXXXV.Lipids(PartB)
EditedbyJohnM.Lowenstein
VolumeXXXVI.HormoneAction(PartA:SteroidHormones)
EditedbyBertW.O’MalleyandJoelG.Hardman
VolumeXXXVII.HormoneAction(PartB:PeptideHormones)
EditedbyBertW.O’MalleyandJoelG.Hardman
VolumeXXXVIII.HormoneAction(PartC:CyclicNucleotides)
EditedbyJoelG.HardmanandBertW.O’Malley
VolumeXXXIX.HormoneAction(PartD:IsolatedCells,Tissues,
andOrganSystems)
EditedbyJoelG.HardmanandBertW.O’Malley
VolumeXL.HormoneAction(PartE:NuclearStructureandFunction)
EditedbyBertW.O’MalleyandJoelG.Hardman
VolumeXLI.CarbohydrateMetabolism(PartB)
EditedbyW.A.Wood
VolumeXLII.CarbohydrateMetabolism(PartC)
EditedbyW.A.Wood
VolumeXLIII.Antibiotics
EditedbyJohnH.Hash
VolumeXLIV.ImmobilizedEnzymes
EditedbyKlausMosbach
VolumeXLV.ProteolyticEnzymes(PartB)
EditedbyLaszloLorand
VolumeXLVI.AffinityLabeling
EditedbyWilliamB.JakobyandMeirWilchek
VolumeXLVII.EnzymeStructure(PartE)
EditedbyC.H.W.HirsandSergeN.Timasheff
VolumeXLVIII.EnzymeStructure(PartF)
EditedbyC.H.W.HirsandSergeN.Timasheff
VolumeXLIX.EnzymeStructure(PartG)
EditedbyC.H.W.HirsandSergeN.Timasheff
VolumeL.ComplexCarbohydrates(PartC)
EditedbyVictorGinsburg
VolumeLI.PurineandPyrimidineNucleotideMetabolism
EditedbyPatriciaA.HoffeeandMaryEllenJones
VolumeLII.Biomembranes(PartC:BiologicalOxidations)
EditedbySidneyFleischerandLesterPacker
VolumeLIII.Biomembranes(PartD:BiologicalOxidations)
EditedbySidneyFleischerandLesterPacker
xviii methods in enzymology
VolumeLIV.Biomembranes(PartE:BiologicalOxidations)
EditedbySidneyFleischerandLesterPacker
VolumeLV.Biomembranes(PartF:Bioenergetics)
EditedbySidneyFleischerandLesterPacker
VolumeLVI.Biomembranes(PartG:Bioenergetics)
EditedbySidneyFleischerandLesterPacker
VolumeLVII.BioluminescenceandChemiluminescence
EditedbyMarleneA.DeLuca
VolumeLVIII.CellCulture
EditedbyWilliamB.JakobyandIraPastan
VolumeLIX.NucleicAcidsandProteinSynthesis(PartG)
EditedbyKivieMoldaveandLawrenceGrossman
VolumeLX.NucleicAcidsandProteinSynthesis(PartH)
EditedbyKivieMoldaveandLawrenceGrossman
Volume61.EnzymeStructure(PartH)
EditedbyC.H.W.HirsandSergeN.Timasheff
Volume62.VitaminsandCoenzymes(PartD)
EditedbyDonaldB.McCormickandLemuelD.Wright
Volume63.EnzymeKineticsandMechanism(PartA:InitialRateand
InhibitorMethods)
EditedbyDanielL.Purich
Volume64.EnzymeKineticsandMechanism(PartB:IsotopicProbesand
ComplexEnzymeSystems)
EditedbyDanielL.Purich
Volume65.NucleicAcids(PartI)
EditedbyLawrenceGrossmanandKivieMoldave
Volume66.VitaminsandCoenzymes(PartE)
EditedbyDonaldB.McCormickandLemuelD.Wright
Volume67.VitaminsandCoenzymes(PartF)
EditedbyDonaldB.McCormickandLemuelD.Wright
Volume68.RecombinantDNA
EditedbyRayWu
Volume69.PhotosynthesisandNitrogenFixation(PartC)
EditedbyAnthonySanPietro
Volume70.ImmunochemicalTechniques(PartA)
EditedbyHelenVanVunakisandJohnJ.Langone
Volume71.Lipids(PartC)
EditedbyJohnM.Lowenstein
