MORPHOLOGICAL CHARACTERIZATION OF COWPEA

133 Pages·2016·2.62 MB·English

MORPHOLOGICAL
CHARACTERIZATION OF COWPEA
[Vigna unguiculata (L.) Walp]
GENOTYPES

MADASU BRAHMAIAH

B.Sc. (Ag.)

MASTER OF SCIENCE IN AGRICULTURE

(SEED SCIENCE AND TECHNOLOGY)

2013
MORPHOLOGICAL CHARACTERIZATION OF
COWPEA [Vigna unguiculata (L.) Walp]
GENOTYPES

BY

MADASU BRAHMAIAH
B.Sc. (Ag.)

THESIS SUBMITTED TO THE
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE
AWARD OF THE DEGREE OF

MASTER OF SCIENCE IN AGRICULTURE

(SEED SCIENCE AND TECHNOLOGY)

CHAIRPERSON: Dr. K. JHANSI RANI

DEPARTMENT OF SEED SCIENCE AND TECHNOLOGY
COLLEGE OF AGRICULTURE
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
RAJENDRANAGAR, HYDERABAD-500 030

2013
DECLARATION

I, MADASU BRAHMAIAH, hereby declare that the thesis entitled
“MORPHOLOGICAL CHARACTERIZATION OF COWPEA [Vigna
unguiculata (L.) Walp] GENOTYPES” submitted to the Acharya N. G. Ranga
Agricultural University for the degree of Master of Science in Agriculture is the
result of original research work done by me. It is further declared that the thesis or any
part thereof has not been published earlier in any manner.

Date: (M.BRAHMAIAH)
Place: Hyderabad I. D. No. RAM/11-39
CERTIFICATE

Mr. MADASU BRAHMAIAH has satisfactorily prosecuted the course of
research and that the thesis entitled “MORPHOLOGICAL CHARACTERIZATION
OF COWPEA [Vigna unguiculata (L.) Walp] GENOTYPES” submitted is the result
of original research work and is of sufficiently high standard to warrant its presentation
to the examination. I also certify that neither the thesis nor its part thereof has been

previously submitted by him for a degree of any university.

Date: /09/2013 (Dr. K.JHANSI RANI)
Chairperson
CERTIFICATE
This is to certify that the thesis entitled “MORPHOLOGICAL
CHARACTERIZATION OF COWPEA [Vigna unguiculata (L.) Walp]
GENOTYPES” submitted in partial fulfilment of the requirements for the degree of
‘Master of Science in Agriculture’ of the Acharya N.G. Ranga Agricultural
University, Hyderabad, is a record of the bonafide research work carried out by Mr.
MADASU BRAHMAIAH under our guidance and supervision. The subject of the
thesis has been approved by the Student's Advisory Committee.
No part of the thesis has been submitted by the student for any other degree or
diploma. The published part and all assistance received during the course of the
investigation have been duly acknowledged by the author of the thesis.

(Dr. K.JHANSI RANI)
Chairperson of the Advisory Committee

Thesis approved by the Student Advisory Committee

Chairperson Dr. K. JHANSI RANI
Associate professor
Department of Seed Science and Technology
College of Agriculture, ANGRAU
Rajendranagar, Hyderabad-500 030

Member Dr. N. SUNIL
Sr. Scientist (Genetics and Plant Breeding)
Winter Nursery Scheme, DMR, ARI
Rajendranagar, Hyderabad-500 030

Member Dr. K. KESHAVULU
Associate Professor and University Head
Department of Seed Science and Technology
College of Agriculture, ANGRAU
Rajendranagar, Hyderabad-500 030

Date of final viva-voce:
ACKNOWLEDGEMENTS

No creation in this world is a solo effort, neither is this thesis. i would like to
take this opportunity to acknowledge all, who supported me directly or indirectly,
bringing this thesis to a really good shape.
Firstly, I thank the almighty GOD for his love and blessings, without which I
would not have been able to complete my studies hitherto and present this piece of
work.
I am pleased to place my profound etiquette to Dr.K.Jhansi Rani, Associate
Professor, Department of Seed Science and Technology, Rajendranagar, Hyderabad
for her scholastic guidance, unceasing interest, constructive criticism, technical
guidance, scientific view, cherishable counselling, warmer affection, caring nature,
moral support and also her help in bringing out this thesis. It was really a great
pleasure and privilege for me to be associated with her during my M.Sc. Degree
programme.
I sincerely extend my profound gratitude and appreciation to the member of my
advisory committee Dr. N. Sunil, Scientist (Economic Botany), NBPGR, Regional
Station, Rajendranagar, Hyderabad, for his assistance during the course of
investigation and for providing necessary facilities to carry out the research work and
also affectionate counseling that positively inspired me to finish the task successfully.
I deem it my privilege in expressing my fidelity to Dr.K.Keshavulu, Associate
Professor, Department of Seed Science and Technology, Rajendranagar, Hyderabad
and member of my advisory committee for his valuable suggestions during the course
of my study and for his amiable approach to embellish the study.
With respectful regards and indebtedness I proffer my deep sense of gratitude
and heartfelt thanks to Director, NBPGR, Regional Station, Rajendranagar,
Hyderabad for providing the facility for this research work and help extended during
the course of my study.
I extend my deep sense of gratitude to my parents Sri. M. Sivaiah and Smt.
Thirupathamma and sister Padmavathi teachers B. Kasi Reddy and K. Subba Rao
brothers, Siva, Saida Rao, Srinu, Venkateswarlu for their unyielding love, support and
their belief throughout my educational career.
I sincerely extend my profound gratitude and appreciation to Dr. P.Sujatha,
Assistant Professor, Dr. Razia Sultana, Associate Professor, Amtul, Research
Associate, Venkatesh and Kavitha Department of Seed Science and Technology,
College of Agriculture, ANGRAU, Rajendranagar, Mr. Jayaram and Prasanthi
Research Associates, NBPGR, Regional Station, Rajendranagar, Hyderabad, and Mr.
Praveen, NBPGR, Regional Station, Rajendranagar for their help during the course of
my research.
It is time to surface out genuflect love and affectionate gratitude to my friends,
Amarnath, Lakshmi Prasad, Sandeep, Venu, Omkar, Meghanath, Bhakthavatslam,
Bujji, Sudha, Satya, Nithya, Susmita, Ravi Kiran, Anil, Srikanth, Lalith, Jitendra
seniors Uday Bhaskar, Shivani madam, Vidya Kumari madam, Sravani madam,
Sooganna, Raju, Anil Kumar, Rajesh, Sadaiah, Rajendar, Shiva Krishna, Uday,
Shiva Prasad and juniors Bhagya Sri, Bhanu, Gopi, Satish, Sekhar, Hari, Kumar,
Sunitha, Janaki,… who constantly inspired, educated, guided and moulded me into
present position and whose encouragement brings out my best in every walk of
endeavors including the present research.
I affectionately acknowledge the timely help and cooperation received from all
the staff and field staff and labour, NBPGR Regional Station, Rajendranagar, who
were the heart and soul of this research programme.
I am greatly thankful to the Government of Andhra Pradesh for providing
financial assistance during the course of my investigation and Acharya N.G Ranga
Agricultural University for giving me this opportunity to pursue my Post Graduation
study.

Date: /09/2013
Place: Hyderabad (M.BRAHMAIAH)
LIST OF CONTENTS

Chapter No. Title Page No.

I INTRODUCTION

II REVIEW OF LITERATURE

III MATERIAL AND METHODS

IV RESULTS AND DISCUSSION

V SUMMARY AND CONCLUSIONS

LITERATURE CITED
LIST OF TABLES

Table Title Page
List of cowpea accessions included for the present study
3.1
Scoring of qualitative traits for varietal identification among cowpea
4.1
genotypes
4.2 Classification of cowpea genotypes based on early plant vigour
4.3 Classification of cowpea genotypes based on plant growth habit
4.4 Classification of cowpea genotypes based on flower colour
4.5 Classification of cowpea genotypes based on immature pod colour
4.6 Classification of cowpea genotypes based on eye pattern
4.7 Classification of cowpea genotypes based on seed coat colour
4.8 Classification of cowpea genotypes based on seed shape
Classification of cowpea genotypes based on days to 50 %
4.9
flowering
4.10 Classification of cowpea genotypes based on leaf length
4.11 Classification of cowpea genotypes based on leaf width
4.12 Classification of cowpea genotypes based on plant height
Classification of cowpea genotypes based on number of primary
4.13
branches
4.14 Classification of cowpea genotypes based on length of main branch
Classification of cowpea genotypes based on number of clusters per
4.15
plant
4.16 Classification of cowpea genotypes based on peduncle length
4.17 Classification of cowpea genotypes based on pod length
4.18 Classification of cowpea genotypes based on pods per plant
Classification of cowpea genotypes based on days to 80 %
4.19
flowering
Classification of cowpea genotypes based on number of seeds per
4.20
pod
4.21 Classification of cowpea genotypes based on seed yield
4.22 Classification of cowpea genotypes based on 100-seed weight
4.23 Classification of cowpea genotypes based on germination per cent
Classification of cowpea genotypes based on seedling vigour index-
4.24
I
4.25 Classification of cowpea genotypes based on seedling vigour index-II
4.26 Classification of cowpea genotypes based on protein content
4.27 Analysis of variance for quantitative characters in cowpea
4.28 Mean performance of 40 genotypes of cowpea
4.29 Genetic parameters for quantitative traits among cowpea genotypes
Promising accessions of cowpea for major yield components
4.30
Phenotypic correlation coefficient among seventeen yield and yield
4.31
related attributes in 40 genotypes of cowpea
Direct and indirect effects of component characters on seed yield in
4.32
40 genotypes of cowpea
Distribution of cowpea genotypes in different clusters based on
4.33
analysis of morphological data
Average intra (bold) and inter cluster D2 values for six clusters in 40
4.34
genotypes of cowpea
Contd..
4.35 Cluster mean values for quantitative characters in cowpea genotypes
Relative contribution of each character towards genetic divergence
4.36
in cowpea

LIST OF ILLUSTRATIONS

Fig. Title Page
PCV and GCV for eighteen characters in cowpea genotypes
4.1
Heritability and genetic advance as per cent of mean for eighteen
4.2
characters in cowpea genotypes
Simple path diagram representing direct and indirect effects on seed
4.3
yield in cowpea
Dendrogram showing clustering pattern for divergence of cowpea
4.4
genotypes

LIST OF PLATES

Plate Title Page
Characterization of the cowpea genotypes based on morphological
3.1
traits in the field
3.2 Determination of germination of cowpea accessions
Measurement of total seedling length of cowpea accessions for
3.3
calculating seedling vigour index-I
Variation observed in plant growth habit among the cowpea
4.1
genotypes (erect, semi-erect and bushy type)
Variation observed in flower colour among the cowpea genotypes
4.2
(light purple and purple)
Variation observed in immature pod colour among the cowpea
4.3 genotypes (green, light green, dark green, dark green with purple
splashes)
Variation observed in eye pattern among the cowpea genotypes
4.4
(brown small eye, black small eye and black medium eye)
Variation observed in the seed coat colour among the Cowpea
4.5
genotypes
Variation observed in seed shape among the cowpea genotypes
4.6
(kidney, ovoid, globose and rhomboid)