Molecular Sample Preparation Products - Harvard Apparatus

82 Pages·2010·11.65 MB·English

2010/11 molecular
Sample
Preparation
Products
Packed Pipette Tips
SpinColumns™
Filtration Products
DIALYZERS™
Liposomes
CoZap™
ElectroPrep™ System
Equilibrium DIALYZERS™
CPK® Atomic Models
Call to receive other catalogs of interest:
Molecular
Sample
Preparation
CellBiology Physiology Behavioral Micro/Nano Electro-
& & Research Fluidics poration
Electro- Pharma- &
physiology cology
Electrofusion
table of contents
Sample Preparation Table of Contents
Item Page
PrepTips™,TipColumns™&SpinColumns™,
Introduction 3 seepages11-20
Guides:Applications,Technology&Products 4-10
PackedPipetteTips
PrepTips™ 12-14
ProTips™ 15
TipColumns™ 16-17
SpinColumns™
SingleSpinColumns 19-20&22
96-Welland384-WellSpinColumns 21-22
FiltrationProducts 23-24 96-WellSpinColumn,
Re-UsableDAILYZERS™ seepage21
DIALYZERS 26-27
Ultra-FastDIALYZERS 28-29
SpinDIALYZERS 30-31
Flow-ThruDIALYZERS 32-33
SingleUseDIALYZERS™
Ultra-MicroDispoDIALYZERS 35
MicroDispoDIALYZERS 36
FastMacroDispoDIALYZERS 37
96-WellDispoDIALYZERS 38 FastMicro-Equilibrium
DIALYZERS™,
Liposomes 39-40
seepage50
CoZap™41
ElectroPrep™System 42-48
Re-UsableEquilibriumDIALYZERS™
FastMicro-EquilibriumDIALYZERS 50-51
Multi-EquilibriumDIALYZERS 52
SingleUseEquilibriumDIALYZERS™
DispoEquilibriumDIALYZERS 53
96-WellDispoEquilibriumDIALYZERS 54
96-WellDispoEquilibrium
HarvardApparatusCPK®AtomicModels 55
DIALYZERS™,
SpecificationsandAssembly 56 seepage54
GuidetoCPK®AtomicModels 57-58
SummaryofSetswithIndividualAtoms 59
SummaryofFullyAssembled
MolecularModels 60
SummaryofUnassembledModels,
Components&Kits 61
CarbonAtoms 62-63
HydrogenAtoms 64
HalogenAtoms 65 HarvardApparatusCPK®
AtomicModels
NitrogenAtoms 66-67 seepages55-81
OxygenAtoms 68
Phosphorus,Sulfur&MetalAtoms 69
ConnectorLinks 70
Accessories&Tools 71
ResearchandTeachingSets 72-73
NucleicAcidHelices,Components&Sets 74-79
FullyAssembledMolecularModels 80-81
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introduction
The Sample Preparation
Targetmaterialsinbiologicalsamplesarecommonly instrumentalmethodslikeMSmaynotallowdirect
presentintraceamountsamonglargerquantitiesof identificationofspecificmoleculesduetohighbackground
numerousimpuritiesorcomponentssuchas: signalsfromcontaminatingmoleculesinthesample.
• Salts Thereiscurrentlynosinglemethodofpurificationthatis
• Buffers applicabletoallsamples. Appropriatesamplepreparation
methodsareuseddependingonthetypeoftargetmaterial
• Detergents
andthesamplecomposition.
• PolyacrylamideGels
ThisiswhytheHarvardApparatusSamplePreparation
• ExcessDyes
Groupprovidesabroadrangeofsolutionsforyoursample
• Radiolabels preparationneeds. Forexample,ourequilibriumdialysis
• Smallundesiredmoleculeslikeprimers,peptides, productsarewidelyusedformeasuringligandbinding
inhibitors&drugs betweenplasmaproteinsanddrugsordrugcandidates,Free
Theseimpuritiesorcomponentsmayhavetobecompletely T4,FreeT3, FreeCortisol,FreeTestosterone,tonameafew.
removedbeforeanalysis. Thefollowingpageslistthesummary,featuresand
Sometargetmaterialsmustalsobeenrichedbeforethey orderinginformationfortherangeofMolecularSample
canbequantifiedorcharacterized,forexample,bymass PreparationProductsavailablefromtheHarvardApparatus
spectrometry.Withoutsamplepreparation,some SamplePreparationGroup.
HarvardApparatus– HarvardApparatus– HarvardApparatus–
SamplePreparationSolutions SamplePreparationTools CPKModels
Matrix
SPE: Solid Phase Extraction Themostaccuratemolecularmodels
Applications available!CPKmodelsarethe
Tips
AmericanSocietyofBiological
• TraceEnrichment
• PrepTips Chemistssanctionedmodels.Theyare
• De-salting
• ProTips accurateinsize,bondanglesandbond
• BufferExchange • TipColumns flexibility.
• RemovalofSurfactants SpinColumns ThesemodelsareIdealfor:
• RemovalofDetergents/SDS
• Single:Ultra-Micro,Micro,Macro • Courtroommodelstoexplain
• RemovalofUnwantedMolecules chemicalstructures
• 96-Well:Micro,Macro
• Concentrating • Teachingstudentsmolecular
• 384-Well
• DestainingGels structure/activitystudies
• Fractionation/Purification Filtration • Whenmodelsareneededfornew
drugpresentations
• BindingStudies Dialysis
• Verifyingconfigurationscreatedby
Advantages Re-Usable(PTFE) computermodelspackages
• SingleAnalysis
Speed
Disposable
• SingleAnalysis
Load Sample • 96-Well
Volume
LiposomePrep
CoZap
Resolution Ease-of-use Electro-dialysis
Equilibrium Dialysis
SampleRecovery Re-Usable(PTFE)
• SingleAnalysis
• 20SampleAnalysis
Disposable
• SingleAnalysis
3
• 96-Well
sample preparation
Application Guide for Sample Preparation
of Biological Mixtures
i.e.Proteins,NucleicAcids, Chromato- Dialysis Equilibrium ElectroPrep Liposomes CoZap
Carbohydrates graphic Dialysis
AcrylamideRemoval •
AffinityPurification •
AntibodyProduction •
BufferExchange • •
CancerTherapy •
CarbohydratePurification •
Cell-CellInteractions •
Cosmetics •
CsCIRemoval •
DestainingGels •
DetergentRemoval • • •
Diagnostics •
DNABindingAssays •
DrugDelivery •
DrugEntrapmentStudies •
DyeRemoval • • •
Electrophoresis •
EnzymeReplacementTherapy •
ExtractionfromGels •
GeneTherapy •
Glycoprotein/GlycopeptidePurification •
Immunoblotting •
InVitroCell-LiposomeInteractionStudies •
LigandBindingAssays •
LipidPurification •
MediatedDeliveryofMacromolecules •
MetalChelationTherapy •
NickTranslation •
PCRCleanup • •
PeptideRemoval • • •
PlasmidPurification •
PrimerRemoval • •
ProteinBindingAssays •
ProteinPurificationforHPLC/HPCE/GC • • •
ProteinPurificationforMassSpectroscopy • • •
(MALDI,GC/MS,MS-NMR,ESI-MS)
Protein-DrugBindingAssays •
Protein-ProteinInteractions •
PurificationofSampleswithUnknown •
IsoelectricPoints
Pyridoxal-5-PhosphateRemoval •
RadiolabelRemoval • •
RadiopharmaceuticalMarkerTracing • •
ReceptorBindingAssays •
ReconstitutionExperiments(forIonTransport) •
SaltRemoval • • •
SampleConcentration • • •
SDSRemoval • • •
SilicateRemovalafterChromatography •
SizeFractionation • •
SmallMoleculeRemoval • • •
Time-DependentReleaseStudies •
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sample preparation
Technology Selection Guide
ChromatographyMethods Dialysis ElectroDialysis Equilibrium
(i.e.,TipColumns,PrepTips,ProTipsandSpinColumns) Aphysicalseparation Aphysicalseparation Dialysis
Physicalseparationmethods,suchas: methodinwhichsmall methodinwhichthe Aspecificapplicationof
moleculespassthrough movementofthe dialysiswhichisusedto
Hydrophilic-Moleculesareboundtoafunctionalgroupona theporesofasize moleculesthrougha measuretheamountsof
matrixintheorganicphaseandelutedintheaqueousphase; selectivemembrane semi-permeable ligandboundto
Hydrophobic-Moleculesareboundtoafunctionalgroupona whilethelarger membraneis macromoleculesandthe
moleculesareretained acceleratedbyan freeligandpresentin
matrixintheaqueousphaseandelutedintheorganicphase; inthedialysisbagor electricfieldsothat twocompartments
GelFiltration-Thesmallermoleculesdiffuseintotheporesof chamber.Inorderto moleculescanbe separatedbyadialysis
thesupportmaterialmakingtheirpathandelutiontimelonger drivetheequilibrium separatedquicklyby membrane.The
thanthelargermoleculesthatareexcludedontheoutsideofthe mechanismthevolume chargeandsize.This concentrationsofligand
particle.Hencethelargermoleculeselutefirstandthesmaller ontheoutsidemustbe techniqueisusefulin provideinformationon
about200xthevolume separation,collection variousbinding
oneselutelater; oftheretainedvolume andfractionationof parameterssuchas
IonExchange-Moleculesareboundtoanionicfunctional anddevoidofthesmall largeandsmallionic bindingconstants
grouponamatrixbycharge. moleculesofinterest. molecules. bindingcapacityandthe
numberofbindingsites.
Absorption SizeExclusion IonExchange
1 Methods Moderateto Minimal Moderateto Minimal Minimalindialysis, Minimal
Development Considerable Considerable sizeonlymode;
Time moderateto
complexinsizeand
chargemode
2 AverageTime/Speed 5minutes 5minutes 5minutes 1to24hours 3to5minutes 3to24hrs
ofSeparation
3 AbilitytoCollect Excellent Good Excellent Poor Excellent Fair
SpecificSample
Fractions-Small
Molecules
4 AbilitytoCollect Good Good Good Good Good Good
SpecificSample
Fractions-Large
Molecules
5 AbilitytoSeparate Excellent Fair Excellent Poor Excellent Poor
MoreThanOneSet
ofComponents
Simultaneously
6 SampleVolumes afewmicrolitersto afewmicroliters to afewmicroliters to afewmicroliters to afewmicroliters to 96well50to200µl
(nonflowthru >1ml >5ml >5ml >5ml >1ml
versions)
7 NumberofSamples 1to96 1to96 1to96 1to96 1 1to96
RunSimultaneously
8 Materials Mediumtohigh Mediumtohigh Mediumtohigh Mediumtohigh Mediumtohigh Mediumtohigh
Compatibilityfor
MaximumActivity
Recovery
9 AuxiliaryEquipment centrifuge,pipettor centrifuge,pipettor centrifuge,pipettor Stirrer,temperature 200VDC,100mA 200VDC,100mA
controller powersupply,rotator powersupply,rotator
10 Sterilizable CHEMICALLY CHEMICALLY CHEMICALLY CHEMICALLY CHEMICALLY CHEMICALLY
11 SuitableforOrganic YES YES YES YES YES YES
&AqueousSolutions
12 SuitableforHigh YES YES YES YES YES YES
Throughput
Screening(HTS)
RecommendedLiterature ElectroDialysis
SpinColumns Zuo,Xun&Speicher,David;“AMethodforGlobalAnalysisofComplexProteomsUsing
SamplePrefractionationbySolutionIsoelectrofocusingPriortoTwo-Dimensional
SajithA.JayasingheandRalfLangen;“IdentifyingStructuralFeaturesofFibrillarIslet
Electrophoresis”;AnalyticalBiochemistry,Vol.284,pp.266-278,2000.
AmyloidPolypeptideUsingSite-directedSpinLabeling”,JournalofBiologicalChemistry,Vol.
279,No.46,pp.48420-48425,2004. EquilibriumDialysis
Dialysis 1)Kariv,Hong,&Oldenburg;“DevelopmentofaHighThroughputEquilibriumDialysis
Method”;JournalofPharmaceuticalSciences;Vol.90,No.5,pp.580-587, 2001.
JamesB.Ames,AlexanderM.Dizhoor,MitsuhikoIkura,KrzysztofPalczewski,andLubert
2)GuidetoEquilibriumDialysis
Stryer;“Three-dimensionalStructureofGuanylylCyclaseActivatingProtein-2,aCalcium-
sensitiveModulatorofPhotoreceptorGuanylylCyclases”, TheJournalofBiological
Chemistry,Vol.274,No.27,pp.19329-19337,1999.
5
Footnote:MembranesareusedinDialysis,ElectroDialysisandEquilibriumDialysisandcanbedamagedbyhightemperature.
sample preparation
Guide to Sample Preparation Products
Ultra-MicroPrepTip™(U.S.Patent6,537.502) Ultra-Micro/Micro/MacroProTip™
Single-Use Disposable Chromatographic Products
Tips
Ultra-MicroPrepTip™ Ultra-MicroProTip™ MicroProTip™ MacroProTip™
Arevolutionaryfritless Containahydrophilicpolymermaterialwhichbinds5to10x
chromatographicdevice. moreproteinthansilica,seepage15
Doesseparationwithout
clogging,seepage12-14
SampleVolumes(µl) 1-10 1-10 10-100 100-500
TipVolumes(µl) 10 10 200 1,000
BindingCapacity(µg) 0.5 0.2 1.5 9.0
PackingMaterials
Gelfiltration(V0=KD)(Sephadex45-90microns)
G10=(700D)
G25=(5KD)
G50=(30KD)
G100=(100KD)
P2=(2KD)
P6=(6KD)
Hydrophobic(particlesize15-20microns)
C18 •
C8 •
C4 •
Hydrophillic
Silica
CN
NH2
PVOH* • • •
PHEA**
Ionexchange(Sepharose):Q=QuaternaryAmine;DEAE=cross-linkedDiethylaminoethyl;SP=Sulphopropyl;CM=Carboxymethyl12µm;300A
StrongAnionQ
StrongCationSP
WeakAnionDEAE
WeakCationCM
Ionexchange(SilicaBased:OrganicCompatible)PEI=linearPolyethyleneimine;SA=SulfoethylAspartamide;AA=AsparticAcid20µm,300Å
StrongCationSA
WeakAnionPEI
WeakCationAA
MiscellaneousIMAC=(Imidodiacetateoncrosslinkeddivinylbenzene)
ActivatedCharcoal
Cellulose
DetergentRemoval
IMAC
*Polyvinylalcohol **PolyhydroxyethylAspartamide
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sample preparation
Guide to Sample Preparation Products
Ultra-Micro/MicroTipColumn™ Ultra-Micro/MicroSpinColumn™ MacroSpinColumn™ 96-Welland384-Well
SpinColumn™
Single-Use Disposable Chromatographic Products
Tip Columns SpinColumns
Ultra-Micro Micro Ultra-Micro Micro Macro 384-Well 96-Well& 96-WellMacro
TipColumn™ TipColumn™ SpinColumn™ SpinColumn™ SpinColumn™ SpinColumn™ Micro SpinColumn™
SpinColumn™
Awideselectionofpre-packed Awideselectionofpre-packed A96-or384-wellplateforHTSsampleprep,
chromatographicmaterialsin chromatographicmaterialsinSpinColumn seepages21-22
pipettetipformats,seepage16-17 formats,seepages18-20&22
SampleVolumes(µl) 10-25 25-75 10-25 25-75 75-150 10-25 25-100 25-150
TipVolumes(µl) 300 300 300 300 1,000 140 300 400
BindingCapacity(µg) 3-30 5-60 3-30 5-60 30-300 3-30 10-100 30-275
PackingMaterials
Gelfiltration(V0=KD)(Sephadex45-90microns)
G10=(700D) • • • • • • • •
G25=(5KD) • • • • • • • •
G50=(30KD) • • • • • • • •
G100=(100KD) • • • • • • • •
P2=(2KD) • • • • • • • •
P6=(6KD) • • • • • • • •
Hydrophobic(particlesize15-20microns)
C18 • • • • • • • •
C8 • • • • • • • •
C4 • • • • • • • •
Hydrophillic
Silica • • • • • • • •
CN • • • • • • • •
NH2 • • • • • • • •
PVOH*
PHEA** • • • • • • • •
Ionexchange(Sepharose):SA=QuaternaryAmine;DEAE=cross-linkedDiethylaminoethyl;SP=Sulphopropyl;CM=Carboxymethyl12mu;300A
StrongAnionQ • • • • • • • •
StrongCationSP • • • • • • • •
WeakAnionDEAE • • • • • • • •
WeakCationCM • • • • • • • •
Ionexchange(SilicaBased:OrganicCompatible)PEI=linearPolyethyleneimine;SA=SulfoethylAspartamide;AA=AsparticAcid20µm,300Å
StrongCationSA • • • • • •
WeakAnionPEI • • • • • •
WeakCationAA • • • • • •
MiscellaneousIMAC=(Imidodiacetateoncrosslinkeddivinylbenzene)
ActivatedCharcoal • • • • •
Cellulose • • • • • • • •
DetergentRemoval • • • • • • • •
IMAC • • • • • • • •
7
*Polyvinylalcohol **PolyhydroxyethylAspartamide
sample preparation
Guide to Sample Preparation Products
DIALYZER™ Ultra-FastDIALYZER™ SpinDIALYZER™/ Flow-ThruDIALYZER™
FastSpinDIALYZER™
DIALYSIS Products
Reusable DIALYSISChambers
DIALYZER™ Ultra-FastDIALYZER™ SpinDIALYZER™ FastSpinDIALYZER™ Flow-ThruDIALYZER™
Drop-indialysis Drop-intwo-sided Drop-indialysis Drop-intwo-sided Continuousflow,high
chamber.10Available dialysischamber. chamberwithbuilt-in dialysischamberwith surfacearea,in-line
chambersizes, 8Availablechamber magnet.8Available built-inmagnet. dialysissystem.
seepage26 sizes,seepage28 chambersizes, 6Availablechamber 5Availablechamber
seepage30 sizes,seepage30 sizes,seepage32
SampleVolumes(µl) 10-5,000 25-5,000 10-1,500 10-1,500 20->10,000
NumberofMembranes 1 2 1 2 1
Materials PTFE PTFE PTFE PTFE PTFE
Membranes(Daorµm)
RegeneratedCelluloseMWCO(Da),Aqueous/Organic
1K • • • • •
2K • • • • •
5K • • • • •
10K • • • • •
25K • • • • •
50K • • • • •
CelluloseAcetateMWCO(Da),Aqueous
100 • • • • •
500 • • • • •
1K • • • • •
2K • • • • •
5K • • • • •
10K • • • • •
25K
• • • • •
50K • • • • •
100K • • • • •
300K • • • •
PolycarbonatePoreSize,(µm)
0.01(100K) • • • • •
0.05(800K) • • • • •
0.1(1.1M)
• • • •
0.6(2.5M) • • • • •
OrderingInformation
Includeschamber Includeschamberand Includeschamberwith Includeschamberwith Includes2chambers,
and1cap(membranes, 2caps(membranes, magnetandcap magnetandtwocaps membranes,fittings,
stirrerplateandstirbar stirrerplateandstirbar (membranes,stirrerplate (membranes,stirrerplate clampandallenwrench
soldseparately), soldseparately), andstirbarsold andstirbarsold (soldwithorwithout
seepage27 seepage29 separately),seepage31 separately),seepage31 pump),seepage33
PTFE=Teflon™;PP=Polypropylene;PE=Polyethylene;PC=Polycarbonate
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sample preparation
Guide to Sample Preparation Products
Ultra-Micro Micro FastMacroDispoDIALYZER™ 96-WellDispoDIALYZER™ Ultra-FastDIALYZER
DispoDIALYZER™ DispoDIALYZER™ forElectroprep™System
DIALYSIS Products
Single Use DIALYSISProducts ReusableChambers
forElectroprep
Ultra-Micro Micro FastMacro 96-Well Electroprep™ FastFlow-Thru
DispoDIALYZER™ DispoDIALYZER™ DispoDIALYZER™ DispoDIALYZER™ System DIALYZER™
Drop-indialysis Drop-intwo-sided Drop-indialysis Drop-intwo-sided Capableof Continuousflow,
chamber.10 dialysischamber. chamberwithbuilt-in dialysischamber separatingbysize singlechambered
Availablechamber 8Availablechamber magnet.8Available withbuilt-inmagnet. andchange. dialysissystem.
sizes, sizes,seepage36 chambersizes, 6Availablechamber 6Available 4Availablechamber
seepage35 seepage37 sizes,seepage38 chambersizes, sizes,seepage47
seepages42-45
SampleVolumes(µl) 1-5 5-100 1,000-10,000 25-300 25-1500 50-1,000
NumberofMembranes 1 1 2 1perwell upto6 2
Materials PC PC PP PP PTFE PTFE
Membranes(Daorµm)
RegeneratedCelluloseMWCO(Da),Aqueous/Organic
1K • • • • •
2K • • • • •
5K • • • • • •
10K • • • • • •
25K • • • • •
50K • • • •
CelluloseAcetateMWCO(Da),Aqueous
100 • • • •
500 • • • •
1K • •
2K • •
5K • •
10K • •
25K
• •
50K • •
100K • •
300K • •
PolycarbonatePoreSize,(µm)
0.01(100K) • •
0.05(800K) • •
0.1(1.1M)
• •
0.6(2.5M) • •
OrderingInformation
Includeschamber Includeschamber Includeschamber Includesplatewith Includeschamber, Includeschamber,
withattached withattached 2lockingringsand membraneand 2caps,membrane, fittingsand2caps
membraneand membrane,flotation 2membranes 12stripcaps tankwithsealand (membranessold
2sampletubes ring,capand (stirrerplateandstir (stirrerplateandstir lidwithleads separately),
(centrifuge 2sampletubes barsoldseparately), barsoldseparately), (powersupplysold seepage48
recommended, (stirrerplateandstir seepage37 seepage38 separately),
soldseparately), barsoldseparately), seepage46
seepage35 seepage36
PTFE=Teflon™;PP=Polypropylene;PE=Polyethylene;PC=Polycarbonate 9
sample preparation
Guide to Sample Preparation Products
FastMicroEquilibrium Multi-Equilibrium DispoEquilibriumDIALYZER™ 96-WellEquilibriumDIALYZER™
DIALYZER™ DIALYZER™
Equilibrium DIALYSIS Products
for binding studies, protein-protein interactions and protein-drug binding assays
Reusable Products Single-use Products
FastMicro-Equilibrium Multi-Equilibrium DispoEquilibrium 96-WellEquilibrium
DIALYZER™ DIALYZER™ DIALYZER™ DIALYZER™
Asinglesamplere-usable Upto20parallelequilibrium ZSinglesample,singleuse Singleuseproductfor96
equilibriumdialysis2-chamber assays.Re-usablechambers productforinteractionstudies. simultaneousassyas.
systemforproteinbinding membraneassemblyrequired, Ready-to-use:Preassembled Ready-to-use:Preassembled
studies.Membraneassembly seepage52 withdialysismembrane,see withdialysismembrane,
required,seepage50 page53 seepage54
SampleVolumes(µl) 19-25 188-250 25-75 50-200
38-50 750-1,000
75-100 1,500-2,00
188-250 3,750-5,000
375-500
NumberofMembranes 1 1/Chamber 1 1/Chamber
StirringDevice No Built-in – Rotator
Materials PTFEBody PTFEChamberDelrin™Plugs PPBodyPECaps PPBodyPECaps
MembranesMWCO(Da)
RegeneratedCellulose RegeneratedCellulose 5,000or10,000 5,000or10,000 5,000or10,000
1,000to50,000
CelluloseAcetate
100to300,000
Polycarbonate
0.01µmto0.60µm
SpecialsAccepted Yes Yes Yes Yes
OrderingInformation
Includes1chamber,1link IncludesDriveUnit, Includeschamberswith Includesplateswith
and2solidcapsperpackage; 1packageof200membranes membrane,2caps membranes,12topstrips
Membranessoldseparately. (10,000MWCO),20emptying (oneblack,onewhite)and and12bottomstrips;
Seepage51 stoppers,4carriers, 2sampletubes(0.65ml) (rotatorsoldseparately),
120plugs,24spacers, perchamber;2specialpipette seepage54
1fillingclampandstand; tipsincluded;(centrifuge
20cells;(Tankfor recommendedandsold
temperaturecontrolavailable separately).Seepage53
asanoption).Seepage52
PTFE=Teflon™;PP=Polypropylene;PE=Polyethylene;PC=Polycarbonate
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