Table Of ContentMICROBIAL BIOTECHNOLOGY
Technological Challenges and
Developmental Trends
MICROBIAL BIOTECHNOLOGY
Technological Challenges and
Developmental Trends
Edited by
Bhima Bhukya, PhD
Anjana Devi Tangutur, PhD
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ABOUT THE EDITORS
Dr. B. Bhima
Bhima Bhukya, PhD, is Head and Assistant Professor
at the Department of Microbiology, Osmania
University, Hyderabad, India. Dr. Bhima, during his
early scientific career, made fundamental contribu-
tions in the area of animal probiotics. Subsequently,
he contributed towards the development of efficient
stress-tolerant probiotic yeast and economically
viable medium for large-scale production of yeast. His present research inter-
ests lie in development of recombinant probiotics using tools of proteomics
and genomics, animal nutrition, biofuels, and bioprocess engineering. He
has fifteen years of post graduate teaching and research experience. He was
awarded the prestigious Raman postdoctoral fellowship/award to carry out
post doctoral studies in the USA.
At present 12 PhD scholars and one post doctoral fellow are working
under his supervision. Dr. Bhima has published twenty research articles in
peer-reviewed journals and four book chapters and has presented over thirty
five papers at various national and international conferences.
He gained experience in the area of animal nutrition-gas production tech-
nology at ICRISAT, Hyderabad, in the area of proteomics at the University
of Hyderabad, Hyderabad, and in the area of plant biotechnology at the
Center for Plant Molecular Biology, Osmania University, Hyderabad. He is
a recipient of several awards, including the HUPO Young Scientist Award
conferred by the World Human Proteome Organization, the SAB-Young
Scientist Award by the Society for Applied Biotechnology, the Research
Excellence award by the Indus Foundation and the state best teacher award
2015 (Young Teacher Award) by the government of Telangana, India. He has
visited countries abroad for scientific interaction and has chaired scientific
sessions during several prestigious national and international conferences.
Dr. Bhima is a life member of AMI, ISCA, BRSI and SAB. He is the
Principal Investigator for several ongoing projects funded by UGC, DBT,
DST etc. He has made significant contributions to university by working
as a warden for PhD scholars hostel and has also been instrumental in
several developmental activities at the department of Microbiology and
vi About the Editors
faculty club of Osmania University. As a convener, Dr. Bhima successfully
organized a three-day national conference on “Recent Trends in Microbial
Biotechnology” and a two-day national seminar on “Advances in Microbial
Technology” in 2015 at Osmania University.
Dr. Anjana Devi Tangutur
Anjana Devi Tangutur, PhD, is a Scientist at Council of Scientific and
Industrial Research-Indian Institute of Chemical Technology (CSIR-IICT),
Hyderabad, India. Dr. Anjana has over ten years of research experience. She
has to date, a brilliant academic career, and pursued her doctoral degree at the
Centre for Cellular & Molecular Biology-CSIR, Hyderabad. Later she joined
the Chemical Biology Division of IICT as a Scientist. During her doctoral
tenure, she has had significant exposure to various molecular biology and
biochemistry approaches and to the mass spectrometry-based proteomics
techniques. Dr. Tangutur's research activities at IICT involve screening of
small molecules as anticancer agents (NFk-β, HDAC inhibitors, antitubulin
agents, etc.) using various in vitro and in vivo tests. Her research interests
lie mainly in applying molecular biology and proteomics based approaches
to understand the underlying mechanisms behind disease pathology (in
different cancers) or a particular mechanism in a biological system. Her
research efforts have been published in reputed international and national
journals and conference proceedings. She was selected for the AOHUPO/
KSMS young scientist award (HUPO 6th Annual World Congress, Seoul,
Korea). She has several ongoing projects funded by DBT, which include the
BioCARe award for women scientists (2013–2016) and also the Rapid grant
for young investigator (2013-2016). She served as a subject expert, external
examiner, and also a member of the several interview selection committees
in colleges, universities, and at CSIR-IICT.
She has co-guided several MPharm/MSc students for partial fulfill-
ment of the post-graduation degree, and presently several qualified research
scholars are pursuing PhD under her guidance. She is also a member of
several editorial boards and a reviewer for several journals.
CONTENTS
List of Contributors ............................................................................xi
List of Abbreviations .........................................................................xv
List of Symbols .................................................................................xxi
Preface ...........................................................................................xxiii
Introduction ......................................................................................xxv
Part I: Antimicrobial Agents: Role and Applications in
Medicine and Healthcare ....................................................................1
1. Antimicrobial Peptides from Plants and Their Application .......................3
B. Bhima and Mohammed Al Saiqali
2. Development of Antitubercular Agents through Hybridization
Strategies: Future Challenges and Perspectives ........................................27
Srinivas Kantevari, Anvesh Jallapally, Anjana Devi Tangutur, and Dinesh Kumar
3. Novel Bioactive Compounds from an Endophytic Chaetomium
arcuatum Strain SAF-2 Isolated from Semecarpus anacardium ...............63
C. Ganesh Kumar, Narender Reddy Godumagadda, Poornima Mongolla,
Sujitha Pombala, Preethi Badrinarayan, Cheruku Ravindra Reddy, Biswanath Das,
K.V.S. Ramakrishna, Balasubramanian Sridhar, and Ahmed Kamal
4. Understanding the Role of Biomarkers in the Pathogenesis of Sepsis .....89
S. Burgula, M. Swathi Raju, A. Nichita, A. Pawan Kumar, and Karthik Rajkumar
5. Role of Copper in Modifying Cisplatin Induced Cytotoxicity in Yeast ....109
B. Sreedhar and B. Vijaya Lakshmi
Part II: Role of Microorganisms in Agriculture and
Plant Biotechnology .........................................................................131
6. Role of Microbiome: Insights into the Kin Recognition Process
in Oryza sativa by Proteomic and Metabolomic Studies ..........................133
Anjana Devi Tangutur, Kommalapati Vamsi Krishna, Amrita Dutta Chowdhury, and
Neelamraju Sarla
viii Contents
7. Associative Effect of Arbuscular Mycorrhizal Fungi and
Rhizobium on Plant Growth and Biological Control of
Charcoal Rot in Green Gram [Vigna radiata L. (Wilczek)] ....................155
A. Hindumathi, B. N. Reddy, A. Sabitha Rani, and A. Narsimha Reddy
8. Morphological Changes in Vigna radiata Root Under
Cadmium Induced Stress in the Presence of Plant
Growth Promoting Enterobacter sp. C1D .................................................171
Rakesh Kumar Sharma, Kavita Barot, and G. Archana
9. Potential Use of Trichoderma Species as Promising Plant
Growth Stimulator in Tomato (Lycopersicum esculantum L.).................185
B. N. Reddy, K. Sarita, and A. Hindumathi
Part III: Microbial Enzymes and Their Potential
Industrial Applications ....................................................................199
10. Strain Improvement of Aspergillus niger for the Enhanced
Production of Cellulase in Solid State Fermentation ...............................201
G. Praveen Kumar Reddy, A. Sridevi, Kanderi Dileep Kumar,
G. Ramanjaneyulu, A. Ramya, B.S. Shanthi Kumari, and B. Rajasekhar Reddy
11. Screening, Quantification, and Purification of Cellulases
from Soil Actinomycetes .............................................................................219
Payal Das, Renu Solanki, and Monisha Khanna
12. Kinetic Studies of Alkali Pretreated Sweet Sorghum
Bagasse Using Cellulase ..............................................................................249
L. Saida and K. Venkata Sri Krishna
13. Secretion of Ligninolytic Enzymes by the White Rot Fungus
Stereum ostrea Immobilized on Polyurethane Cubes Under
the Influence of Chlorpyrifos .....................................................................265
B. S. Shanthi Kumari, K. Dileep Kumar, K. Y. Usha, A. Ramya, and B. Rajasekhar Reddy
14. Structure and Gas Diffusion Path Analysis of Hydrogenase
Enzymes by Homology Modelling .............................................................277
Nivedita Sahu, Anirudh Nelabhotla, and Pradhan Nityananda
Part IV: Microorganisms in the Environment: Role and
Industrial Applications ....................................................................295
15. Yeast Saccharomyces cerevisiae as an Efficient Biological Agent
for Decolorization of Reactive Dyes used in Textile Industry .................297
B. Bhima, N. Hanumalal, and B. Chandrasekhar
