Table Of Contentइंटरनेट मानक
Disclosure to Promote the Right To Information
Whereas the Parliament of India has set out to provide a practical regime of right to
information for citizens to secure access to information under the control of public authorities,
in order to promote transparency and accountability in the working of every public authority,
and whereas the attached publication of the Bureau of Indian Standards is of particular interest
to the public, particularly disadvantaged communities and those engaged in the pursuit of
education and knowledge, the attached public safety standard is made available to promote the
timely dissemination of this information in an accurate manner to the public.
“जान1 का अ+धकार, जी1 का अ+धकार” “प0रा1 को छोड न’ 5 तरफ”
Mazdoor Kisan Shakti Sangathan Jawaharlal Nehru
“The Right to Information, The Right to Live” “Step Out From the Old to the New”
IS 13663 (1993): Chilli Oleoresin [FAD 9: Spices and
Condiments]
“!ान $ एक न’ भारत का +नम-ण”
Satyanarayan Gangaram Pitroda
““IInnvveenntt aa NNeeww IInnddiiaa UUssiinngg KKnnoowwlleeddggee””
“!ान एक ऐसा खजाना > जो कभी च0राया नहB जा सकता हहहहै””ै”
Bhartṛhari—Nītiśatakam
“Knowledge is such a treasure which cannot be stolen”
IS 13663 : 1993
(Reaffirmed - 2012)
&wT ?TT fG-4- Rfwz
Indian Standard
CHILL1 OLEORESIN - SPECIFICATION
UDC 664-521.1
@ BIS 1993
BUREAU OF INDIAN STANDARDS
MANAK BHAVAN, 9 BAHADUR SHAH ZAFAR MARG
NEW DELHI 110002
February 1993 Price Group 2
secipS and stnemidnoC lanoitceS ,eettimmoC FAD 9
FOREWORD
sihT naidnI dradnatS was detpoda yb eht uaeruB fo naidnI Standards, retfa eht tfard dezilanif yb
eht secipS and stnemidnoC lanoitceS eettimmoC had neeb devorppa yb eht dooF and
erutlucirgA noisiviD .licnuoC
ehT nredom egasu fo secips ni doof seirtsudni ni ynam saesrevo seirtnuoc is ni eht mrof fo
.sniseroelo iliihC niseroelo is osla desu rof gniruc gniwehc occabot and as retnuoc tnatirri ni
pain balms.
illihC niseroelo is deniatbo yb eht noitcartxe fo seillihc ( eht tiurf fo der ,reppep
Cupsicum
.L ro .L ) htiw devorppa doof edarg tnevlos and tneuqesbus luferac
annuum Cupsicum frutescens
lavomer fo eht tnevlos yb .noitallitsid sediseB esnetni ycnegnup eud ot niciaspac and smal1
seititnauq fo deilla ,sdiolakla eht illihc niseroelo lliw evah krad der ruoloc eud ot dionetorac
.stnemgip ruoloC and ycnegnup era tnatropmi morf eht tniop fo weiv fo ytilauq fo eht
.tcudorp
ruoloC is denimreted yb latnemurtsni .sisylana roF noitamitse fo niciaspac tnetnoc owt sdohtem
yleman CLT and repaP yhpargotamorhc evah neeb .dedulcni oslA a dohtem desab no yrosnes
noitaulave is nevig rof noitamitse fo .ycnegnup
roF eht esoprup fo gnidiced rehtehw a ralucitrap tnemeriuqer fo this standard is deilpmoc htiw
eht lanif ,eulav devresbo ro ,detaluclac gnisserpxe eht stluser fo a tset ro ,sisylana shall eb
dednuor ffo ni ecnadrocca htiw SI 2 : 1960 seluR‘ rof gnidnuor ffo laciremun seulav ( revised )‘.
ehT rebmun fo tnacifingis secalp deniater ni eht dednuor ffo eulav dluohs eb eht emas as that fo
eht deificeps eulav ni this standard.
5 IS 13663 : 1993
Indian Standard
1LLIHC NISEROELO - NOITACIFICEPS
1 SCOPE 5 MARKING
1.1 sihT dradnats sebircserp the stnemeriuqer 5.1 ehT reniatnoc llahs eb dekram htiw the
dna the sdohtem of gnilpmas dna test for illihc :gniwollof
.niseroelo
a) emaN of the ;lairetam
2 TERMINOLOGY )b s’rerutcafunaM eman dna trade ,kram fi
;yna dna
For the esoprup of siht ,dradnats snoitinifed
nevig rednu 2 of SI 326 : 1968 llahs .ylppa c) Net ssam of the lairetam nehw packed.
6 GNILPMAS
3 REQUIREMENTS
6.1 evitatneserpeR ,selpmas of the ,lairetam
3.1 Description
tneiciffus ot evig a etisopmoc elpmas for -ilpirt
3.1.1 ehT illihc niseroelo llahs eb deniatbo yb cate ,snoitanimreted llahs eb nward morf the
tnevlos noitcartxe of the deird epir stiurF of sreniatnoc detceles morf the tol as debircserp
Capsicum annuum L or Capcicum frutescens L, rednu 3 of SI 326 : 1968.
htiw tneuqesbus lavomer of .tnevlos
6.2 stseT for lla the scitsiretcarahc llahs eb
3.1.2 ehT lairetam llahs eb a red suocsiv diuqil detcudnoc ni the etisopmoc .elpmas
htiw the citsiretcarahc ruodo of ,illihc hgih
ycnegnup dna deep red .ruoloc ehT lairetam 7 STSET
llahs eb free morf ytidicnar dna .ruovalf-ffo
7.1 stseT llahs eb detcudnoc as debircserp ni
3.2 The lairetam llahs osla ylpmoc htiw -eriuqer co1 4 dna 5 of elbaT 1.
stnem nevig ni elbaT 1.
7.2 ytilauQ of stnegaeR
4 PACKING
sselnU deificeps ,esiwrehto erup slacimehc dna
4.1 ehT lairetam llahs eb deilppus ni ylthgit dellitsid water ( (see SI 1070 : 1992 ) llahs eb
desolc ,ssalg erup muinimula or etalpnit
deyolpme ni tests.
sreniatnoc hcihw llahs eb ylraen .lluf
NOTE - eruP‘ ?slacimehc naem slacimehc taht od
4.1.1 ehT lairetam llahs eb protected morf ton niatnoc s<itirupmi hcihw tceffa eht stluser fo
thgil dna stored ni a looc .ecalp .sisylana
Table 1 Requirements for Cbilli Oleoresin
( Clause 3.2 )
scitsiretcarahC tnemeriuqeR Method of ,tseT Ref ot
SI r------*--- ---
No.
xennA fo siht xidneppA fo
dradnatS SI 5832:1975
)1( )2( (3) (4) 15)
9 ruoloC eulav 4 02-000 000 A -
)ii ellivocS taeh ,stinu Min 240 000 B
)iii niciaspaC ,tnetnoc tnecrep yb ,ssam Min 1.5 C -
)vi laudiseR ,tnevlos ,gkjgm MUX : - - C
a) enaxeH 25
)b enelyhte/enotecA enelyhteorolhcirt/edirolhcid 30
,c lonaporposi/lonehteM 30
1
SI 13663 : 1993
ANNEX A
[ Tab/e 1, Item (i) ]
NOITANIMRETED FO RUOLOC EULAV
A-l ENILTUO OF EHT SDOHTEM 3-A STNEGAER
oT ekam the noitamitse yletelpmoc ,evitcejbo 1.3-A enotecA
latnemurtsni sisylana si .deyolpme
COlOLlr Of a A-4 PROCEDURE
deificeps noitulid si detamitse at 458 ,mn the
ecnabrosba gnidaer deilpitlum yb a noitulid 1.4-A hgieW 1.0 g of niseroeIo otni a lm-OOI
factor dna the tluser expressed as -irelesseN cirtemulov ,ksalf dna ekam pu the emulov htiw
cirtem ruoloc .eulav .enoteca ettepiP 0.I lm of siht noitulos otni
a dnoces lm-OOI cirtemulov ksalf dna ekam pu
2-A SUTARAPPA the emulov htiw .enoteca
A-2.1 lOO-ml Volumetric Flasks A-4.2 gnisU a netsgnut pmal ecruos dna
enoteca as the ,knalb take the ecnabrosba of
2.2-A lm-l ettepiP
the 0.01 tnecrep noitulos of niseroelo at
3.2-A retemotohportcepS or a retemirolac htiw 458 .mn ylpitluM siht gnidaer yb 61000 ot
niatbo the ruoloc .eulav
adjustable wave length
ANNEX B
[ Table 1, Item )ii( ]
NOITANIMRETED F.0 ELLIVOCS HEAT UNIT
B-l OUTLINE OF THE METHOD ,gnikahs 5 lm of siht .noitulos A etib or -gnits
gni noitasnes ni the throat hcihw si
sihT si a dohtem desab no yrosnes .noitaulave just
elbitpecr:p at siht noitulid si lauqe ot 240 000
niseroelO snoitulos ylbairav detulid htiw ragus
ellivocs .stinu fI etib si yrev ,gnorts a rehtruf
noitulos are tasted ni gnisaercni .noitartnecnoc
noitulid of siht noitulos as nevig woleb si
ehT tsehgih noitulid nehw a ycnegnup si tsuj
yrassecen for a elbitpecrep tnegnup :noitasnes
detected si nekat as a erusaem of the heat
.eulav a) 15 lm of ragus noitulos
30 lm noitulos ( 2.3-B ) = 360 000
2-B STNEGAER
ellivocS stinu
B-2.1 5 Percent Sugar Solution
b) 20 lm of ragus noitulos
k-2.2 95 Percent Alcohol 30 lm noitulos ( 2.3-B ) = 081~ 000
ellivocS
Uilits
B-3 PROCEDURE
B-3.1 hgieW yletarucca 0.2 g of the niseroelo c) 30 lm ragus noitulos
otni a lm-05 cirtemulov .ksalf ddA lohocla ot 15 lm noitulos ( 2.3-B ) = 720 000
the emulov kram dna shake the stnetnoc dna ellivocS stinu
wolla ot .elttes
ehT lenap for noitaulave llahs tsisnoc of
2.3-B ddA 0.15 lm of cilohocla noitulos ot 5 ,srebmem dna 3 srebmem tuo of 5 llahs dnif
140 lm ragus .noitulos wollawS after hguoroht the noitulos ot eb .tnegnup
SI 13663 : 1993
ANNEX C
)iii(
[ Table 1, Item ]
DETERMINATION OF CAPSAICIN CONTENT
C-l GENERAL C-2.4.1 Preparation of TLC Plates
ehT ycnegnup of iilihc si eud ot niciaspac dna xiM 30 g acilis leg gniniatnoc muiclac etahplus
traces of deilla .slacimehc ycnegnuP nac eb as .rednib xiM htiw 60 lm dellitsid water.
ylevitcejbo detaulave yb gnitamitse the ruoP the yrruls otni a CLT spreader detsujda ot
niciaspac .tnetnoc roF siht ,esoprup two a ssenkciht of 150 nru dna spread revo 5 ot 6
,sdohtem ,yleman CLT dna paper -otamorhc ssalg setalp of 20 x 20 .mc riA yrd the setalp
yhparg are yllauqe .elbatrus for 4 ot 5 ,sruoh retal etavitca yb gniyrd ni na
nevo at C°501-OOI for 30 setunim dna store ni
2-C TLC METHOD a .rotaccised
C-2.1 Outline of the Method 2.4.2-C Estimation
ehT niciaspac dna deilla stneutitsnoc yam eb
C-2.4.2.1 hgieW 5 g niseroelo ni a lm-OI
separated morf other stneutitsnoc yb niht
stoppered gnirusaem raj dna ekam pu the
reyal yhpargotamorhc ( CLT ) no acilis .leg
emulov htiw .enoteca topS 10 lu ( 5 ot 20 lu
ehT tnegnup elpicnirp nac react htiw -niloF
gnidneped nopu the niciaspac tnetnoc ) of siht
sinneD tnegaer ot evig a eulb xelpmoc hcihw
noitulos no CLT etalp dna poleved htiw
yam eb detamitse .yllacirtemirolac
gnipoleved tnevlos ni na lla ssalg rebmahc
hcihw has neeb ylhguoroht detarutas htiw the
2.2-C Apparatus
ruopav of the .tnevlos Expose the etalp for
tuoba 30 setunim ot free ti of the tnevlos dna
C-2.2.1 Complete TLC Set
ylthgil yarps htiw -tohpsohp-cidbylomohpsohp
C-2.2.2 Spectrophotometer or Calorimeter citsgnu .tnegaer Mark the raelc eulb niciaspac
spot ( RfO.16 ) htiw a llams sselniats leets
elbapaC of gniruaem ni the noiger of 660 ot
scoop or alutaps ni the mrof of a elcric -solcne
725 .mn
gni the spot as llew as na area 0.25 mc dnoyeb
3.2.2-C Measuring Jar with Stopper - 10 .lm the spot. Scoop tuo the acilis l:g gniniatnoc
niciaspac ni the dekram area otni a naelc
4.2.2-C Test-Tubes rettub paper dna refsnart otni a test .ebut ddA
3.5 lm dellitsid water dna shake .llew ettepiP
C-2.2.5 Graduated lo-ml Pipettes - htiw 0.1 lm
tuo 0.5 lm citsgnutohpsohp-cidbylomohpsohp
.noitaudarg
tnegaer otni the ebut dna xim .llew retfA 3
3.2-C Reagents setunim add 1 lm detarutas suoeuqa muidos
etanobrac .noitulos xiM ylhguoroht for
C-2.3.1 Silica Gel ( TLC’ Grade ) 5 setunim dna set edisa for 1 .ruoh
2.2.4.2-C Prepare a tnegaer ,knalb gnisu acilis
C-2.3.2 Developing Solvent
leg sreyal morf a knalb area ni the .etalp
erutxiM of 80 lm of enezneb dna 5 lm of egufirtneC the sebut ( or retlif ) at the dne of
.lonahtem eno ruoh ot separate .sdilos Read lacitpo
ytisned ni retemotohportceps at 725 mn or ni
3.3.2-C Phosphomolybdic-Phosphotungstic Acid
a retemirolac gnisu a ssalg retlif ni 660 ot
( Fotin-Dennis Reagent )
690 mn .noiger
xulfeR for 2 ,sruoh 750 lm water, 100 g muidos
3.4.2-C enimreteD the tnuoma of X niciaspac
,etatsgnut 20 g cidbylomohpsohp dica dna 50 lm
ni the spot morf a dradnats evruc of -artnecnoc
cirohpsohp .dica looC the erutxim dna etulid
noit of erup nillinav versus lacitpo .ytisned
ot 1000 lm htiw water.
ylpitluM yb 2 ( a factor ) ot correct for the
ecnereffid ni the ralucelom ssam of niciaspac
4.3.2-C Vanillin - lacitylana grade. dna .nillinav
5.3.2-C Benzene - CLT grade
4.4.2-C Preparation of Standard Curve
6.3.2-C Methanol - CLT grade
tnereffiD semulov of a dradnats suoeuqa
4.2-C Procedure noitulos of nillinav are reached htiw -niloF
3
i‘
SI 13663 : 1993
sinneD tnegaer dna the lacitpo ytisned si 7.3.3-C Gibbs Reagenf
.denimreted
0.1 tnecrep noitulos of 2, -ozneb-p-orolhcid-6
NOTE .- ehT dradnats suoeuqa noitulos fo nillinav enimorolhc-4,noniuq ni enoteca as the
dluohs 10.0niatcoc .lm/gm 1 ot 3.5 lm fo siht cinegomorhc .tnegaer
noitulos tcaer htiw 0.5 lm sinneD-niloF tnegaer dna
1 lm detarntas suoeuqa muidos etanobrac ,noitulos
gnipeek eht latot emulov ot 5 lm yb noitidda fo 4.3-C Procedure
dellitsid .ret.aw
C-3.4.1 evlossiD the niseroelo or sevitcartxe ni
5.4.2-C Draw a dradnats evruc gnittolp ssam of lyhte acetate ot evig a noitulos gniniatnoc
nillinav tsniaga lacitpo .ytisned tuoba 2.5 g/1 of .niciaspac ylppA na tnuoma
of the noitulos gniniatnoc 05-OI gu niciaspac
5.2-C Calculation ( that si 4 ot 20 lu ) as a niht streak gnirevoc
the eritne htdiw of a 2x20 mc pirts of -tahW
niciaspaC ,tnetnoc XX 1 000 x 200
nam No. 3 retlif paper. poleveD yb gnidnecsa
tnecrep yb ssam =
r yhpargotamorhc ni reffub-lonahtem tnevlos
litnu the tnevlos tnorf has devom pu ot tuoba
where 15 ,mc yllausu ni tuoba 1 .ruoh yrD the pirts
ni .ria
X = the rebmun of g niciaspac
( nillinav x 2 ) ni the spot, dna
2.4.3-C Pass the deird paper pirts ylmrofinu
r = the rebmun of lu of 50 tnecrep enoteca hguorht sbbiG tnegaer dna yrd ni .ria niciaspaC
noitulos of .niseroelo semoceb yltniaf elbisiv as a eulb spot raen the
tnevlos .tnorf poIeveD the ruoloc rehtruf yb
C-3 PAPER CHROMATOGRsAPHY METHOD gniyarps htiw reffub ,noitulos ylthgil no htob
sedis of the paper. refsnarT the pirts ot a
C-3.1 Outline of the Method dark draobnuc for 30 setunim for lluf -poleved
The tnegnup selpicnirp yam eb separated morf tnem of ruoloc dna .gniyrd
other, stneutitsnoc yb paper yhpargotamorhc
3.4.3-C esicxE the eulb spot dna etule htiw
dna yeht nac react htiw sbbiG tnegaer ot evig
lonahtem ni the dark. Make pu the detule
a eulb xelpmoc hcihw yam eb detamitse
ruoloc ot a tneinevnoc emulov ( 01-5 lm ) dna
.yllacirtemirolac
erusaem at 615 mn ni a retemotohportceps
tsniaga a tnegaer knalb detule morf the -serroc
2.3-c sutarappA
gnidnop area of a pirts nur ylsuoenatlumis
1.23-~ r:rf gnidnecsa paper pirts tuohtiw the .elpmas etaluclaC the egatnecrep
Clinn7Frr --
.yhpargotamorhc of nicizspac morf a dradnats evruc detcurtsnoc
as nevig ni 5.4.3-C or yb gnisu the :alumrof
2.2.3-c Spcctrophotorneter or Clorimeter - htiw
I tnecrep
worran dnab .retlif
E 046= at 615 .mn
I mc
C-3.3 stnegaeR
4.4.3-c Prepare the niseroelo or latot tc-,rtxe
1.3.3-C lylltE etatecA -- tnevloS of niciaspac or
of the elpmas yb telhxoS noitcartxe of -wop
l;oiiuiid of latot extracts.
dered mucispac htiw a elbatius tnevlos ( lyhte
2.3.3-6 lonnhteM - lacitylanA tnegaer grade acetate ) for 3-5*2 .sruoh retfA lavomer of
,tnevlos thgiew the eudiser dna proceed as
3.3.3-C ciroB lticd, - lacitylal:A tnegaer grade
ni .4.3-C
4.3.3-C PotO$.Y’il/i?l Chlori~s.‘e
5.4.3-C Prepare a noitulos of erup niciaqpac ni
C-3.3.5 BttffL,r Solution lvhtr acetate ( 2 l/g ), spot 10, 20, 3c, 40 ,dna
OS g; of niciaspac ( 52-5 lu ) no spirts of
A e;rini+:tnoc 3.1 g cirzb icica dna 3.7 s
sol:~tio 1 namtahW No. 3 retlif paper, ,poleved ezilausiv
p muissat) ediroihc n! 100 lm dellitsid letaw
the niciaspac spot. dna .etule tcurtsnoC the
!cetsnj;ia ot Hy 9.6 htiw 1 N muidos
dradnats evruc dna
.cdix?rdyh
I tnecrep
6.3.3-C Mcthcnnl-Byffw Solution
E for erup .niciaspac
pFT 9.6 ( 50-+i0 \‘/V ) nT deVf?lOpiilg SOlVCnt. 1 mc
i
Standard Mark
ehT esu fo eht dradnatS Mark is denrevog yb eht snoisivorp fo eht uaeruB of Indian
and eht seluR and snoitalugeR edam .rednuereht ehT dradnatS Mark
Standards Act, 1986
no stcudorp derevoc yb an naidnI dradnatS syevnoc eht ecnarussa that yeht evah neeb
decudorp ot ylpmoc htiw eht stnemeriuqer fo that standard rednu a llew denifed metsys
fo ,noitcepsni gnitset and ytilauq lortnoc hcihw is desived and desivrepus yb SIB and
detarepo yb eht .recudorp dradnatS dekram stcudorp era osla ylsuounitnoc dekcehc yb
SIB rof ytimrofnoc ot that standard as a rehtruf .draugefas sliateD fo snoitidnoc rednu
hcihw a ecnecil rof eht esu fo eht dradnatS Mark yam eb detnarg ot srerutcafunam ro
srecudorp yam eb deniatbo morf eht uaeruB fo naidnI Standards.
