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MEDICAL
INTELLIGENCE
UNIT
Immunophenotypic Analysis
Second Edition
Katalin Pal6czi, M.D., Ph.D., D.Sc.
Professor and Chair of Immunology
Faculty of Health Sciences
Semmelweis University
National Institute of Haematology and Immunology
Budapest, Hungary
Boca Raton London New York
CRC Press is an imprint of the
Taylor & Francis Group, an informa business
IMMUNOPHENOTYPIC ANALYSIS
SECOND EDITION
Medical Intelligence Unit
First published 2005 by Landes Bioscience
Published 2018 by CRC Press
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© 2005 by Taylor & Francis Group, LLC
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No claim to original U.S. Government works
ISBN-13: 978-1-58706-101-l (hbk)
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Library of Congress Cataloging-in-Publication Data
Pal6czi, Katalin.
Immunophenotypic analysis / Katalin Pal6czi. --2nd ed.
p. ; cm. -- (Medical intelligence unit)
Rev. ed. of: Clinical applications ofimmunophenotypic
analysis. cl 994.
Includes bibliographical references and index.
ISBN 1-58706-101-5
I. Immunophenotyping. 2. Hematopoietic stem cell disorders
--Diagnosis. I. Pal6czi, Katalin. Clinical applications of im-
munophenotypic analysis. II. Title. III. Series: Medical intelli-
gence unit (Unnumbered : 2003)
[DNLM: I. lmmunophcnotyping. 2. Bone Marrow--immuno-
logy. 3. Hematopoietic Stem Cells--immunology. 4. Leukemia
--immunology. 5. Lymphoma, Non-Hodgkin--immunology.
QY 250 Pl818i 2004]
RC644.5.P25 2004
616.07' 582--dc2 l
2001004704
To our family members whose patience
and unwavering support have allowed us to share
important new information with those dedicated
to the conquest of malignant disease
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CONTENTS-------------
Preface.....................................................................................................................viii
1. Normal Hematopoietic Cell Differentiation.....................................................1
Gyongyi Szabo and Angela Dolganiuc
Immunologic Marker Analysis of Cells in the Various Stages
of Hematopoietic Differentiation................................................................ 1
International Nomenclature and Functional Characterization
of Immunologic Markers..............................................................................20
2. Application of Monoclonal Antibodies to the Diagnosis
and Classification of Acute Leukemias............................................................29
Νότα Regiczy
More than Routine Immunophenotyping of Acute Leukemias................29
Immunological Classification of ALL............................................................32
Immunophenotypic Classification of Acute Myeloblastic Leukemia .... 37
Biphenotypic Acute Leukemia..........................................................................41
Undifferentiated Acute Leukemia...................................................................42
Minimal Residual Disease in Acute Leukemia.............................................42
Multidrug-Resistance in Acute Leukemia.....................................................43
3. Immunophenotypic Markers in the Diagnosis
of Non-Hodgkin’s Lymphomas.........................................................................48
Andrds Matolcsy
Lineage of Non-Hodgkin’s Lymphomas........................................................48
B-Cell Non-Hodgkin’s Lymphomas...............................................................49
T-Cell Non-Hodgkin’s Lymphomas...............................................................53
4. Clinical Application of Immunophenotyping
in Immunodeficiencies.........................................................................................62
Katalin Pdldczi
Congenital (Primary) Immunodeficiencies...................................................62
Cellular and Combined Immunodeficiency Diseases.................................63
Predominantly Antibody Deficiency Diseases..............................................68
Phagocytic Cell Disorders..................................................................................71
Use of Immunophenotyping in Clinical Evaluation of Patients
with Primary Immunodeficiencies..............................................................73
Human Immunodeficiency Virus Infection and Acquired
Immunodeficiency Syndrome......................................................................74
5. Immunophenotyping in Hematopoietic Stem Cell Transplantation .... 78
Katalin Pdldczi
Use of CD34 for Identification of Hematopoietic Progenitors...............78
Reconstitution of Hematopoiesis Posttransplant and Normal
Hematopoiesis During Ontogeny...............................................................79
Multi-Tissue Potential of HSC.........................................................................86
Index..........................................................................................................................89
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EDITOR
Katalin FiUai, M.D., PK.D., D.Sc.
Professor and Chair of Immunology
Faculty of Health Sciences
Semmelweis University
National Institute of Haematology and Immunology
Budapest, Hungary
E-mail: [email protected]
Chapters 4, 5
= CONTRIBUTORS =
Angela Dolganiuc, M.D., Ph.D.
Department of Medicine
University of Massachusetts Medical School
Worcester, Massachusetts, U.S.A.
Chapter 1
Andras Matolcsy, M.D., Ph.D., D.Sc.
Associate Professor
Department of Pathology
Faculty of Medicine
Pecs University
Pecs, Hungary
E-mail: [email protected]
Chapter 3
Nora Regeczy, M.D., Ph.D.
Membrane Biology
and Immunopathology Research Group
Hungarian Academy of Sciences
National Institute of Haematology
and Immunology
Budapest, Hungary
E-mail: [email protected]
Chapter 2
Gyongyi Szabo, M.D., Ph.D.
Professor
Department of Medicine
University of Massachusettes Medical School
Worcester, Massachusetts, U.S.A.
E-mail: [email protected]
Chapter 1
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PREFACE
The first edition of Clinical Application of Immunophenotypic Analysis
appeared in 1994. This work was intended to be a comprehensive and up-
to-date book developed to serve a diverse group of individuals ranging from
practicing general immunologists, hematologists, students of the field, basic
scientists involved in research of cell surface antigens, as well as practicing
internists and pediatricians. The science and practice of flow cytometry
and immunophenotyping have continued to evolve. With more and more
clinical information and evaluation demonstrating the value of
immunophenotypic analysis, this has become an established clinical proce
dure.
The rapid advancement of this field has necessitated the creation of a
second edition, in order that the book remains current and useful to our
readers. Besides, there is a need to put a great deal of effort into promoting
the value of clinical flow cytometric tests which can be more efficiently per
formed and provide more definitive results than many of the classical proce
dures. This is especially important in areas of clinical immunology and
hematology.
We retained the features of the first edition that were regarded by our
audience as most valuable, but altered the second edition as required to fur
ther enhance the quality of the book. We have incorporated much new in
formation regarding normal immune cell development as well as diagnosis
of acute leukemias, malignant lymphomas and characterization of stem cells
for transplantation. To accommodate these new insights without significandy
increasing the size of the book, we have reduced discussions of historical
debates that are now resolved by experiments.
All chapters have been rewritten and extensively updated to reflect the
explosion of knowledge. Beside the editor four excellent contributors have
been involved in creating the second edition and all the authors are working
leaders in their subjects. Most of the authors confront their clinical prob
lems on a daily basis and have that uniquely urgent spur to keep them at the
cutting edge. As a result of the collective labor of a creative people, we now
have a book, which hopefully fills a real need in one of the most exciting
branches of modern medicine.
The contributors to this edition have done an outstanding job, and
the editor is indebted to them for their diligence, perseverance, and scholarly
presentations.
The Editor
