Table Of ContentContributors to Volume 150
Article numbers are in parentheses following the names of contributors.
Affiliations listed are current.
CLAUDIO ANASETTI (43), Division of -inilC JULIO E. CELIS (12), Department of Medical
cal Research, Fred Hutchinson Cancer Biochemistry, Aarhus University, DK-
Research Center, Seattle, Washington 8000 Aarhus C, Denmark
40189 SALOHClN CHIORAZZI (5), Cornell Univer-
CLARK L. ANDERSON (42), Department of sity Medical College, and Division of
Internal Medicine, The Ohio State Uni- Rheumatology and Clinical Immunology,
versity College of Medicine, Columbus, North Shore University Hospital,
Ohio 01234 Manhasset, New York 03011
M. AMIN ARNAOUT (48), Harvard Medical DRAHCIR B. CLARK (39), Graduate School
School and The Children's Hospital, Bos- of Biomedical Sciences, University of
ton, Massachusetts 51120 Texas Health Science Center at Houston,
SITARTS SAEMARVA (9), Unit( d'Immunocy- Houston, Texas 52077
tochimie, D(partement d'Immunologie, LNDA K. NOTYALC (48), Harvard Medical
Institut Pasteur, 75724 Paris Cedex ,51 School and Dana-Farber Cancer Insti-
France tute, Boston, Massachusetts 51120
JACK R. OTSXTTAB (4), Department of Im- MELVIN COH~ (10), Developmental Biology
munology and Cancer, ehT Research In- Laboratory, The Salk Institute for Biolog-
stitute, Cleveland Clinic Foundation, ical Studies, La Jolla, California 73029
Cleveland, Ohio 60144
ROSERT E. CONE (33, 51), Department of
MASATOSm BEPPU (2), Tokyo College of Pathology, University of Connecticut
Pharmacy, 1-2341 Horinouchi, Hachioji, Health Center, Farmington, Connecticut
Tokyo ,30-291 Japan 23060
ENVLECOJ NAISSOHOOB (11), Clayton Foun-
DANIEL H. DARNOC (40), Subdepartment of
dation Biochemical Institute, Austin,
Immunology, The Johns Hopkins Univer-
Texas 6901-21787
sity School of Medicine, Baltimore, Mary-
MICHAEL BOOKMAN (29), Biological Re- land 50212
sponse Modifiers Program, National
LXEN R. REPOOC (44), Department oflmmu-
Cancer Institute, National Institutes of
nology, Scripps Clinic and Research
Health, Bethesda, Maryland 29802
Foundation, La Jolla, California 73029
WILIAM E. BOWERS (6), The Medical Re-
CY~L C. CURTAIN (35), Division of Bio-
search Institute, The Mary Imogene Bas-
technology, CSIRO, Clayton, Victoria
sett Hospital, Cooperstown, New York
,8613 Australia
62331
SUCRAM BRAUN (43), Department of Medi- NAVA DANA (48), Harvard Medical School
cine, Oregon State University Hospital, and The Children's Hospital, Boston,
Portland, Oregon 10279 Massachusetts 51120
SALGUOD R. CAVENER (55), Department of INNAVOIG DI OTA3d~,S (1), Department of
Molecular Biology, Vanderbilt Univer- Molecular Biology, Vanderbilt Univer-
sity, Nashville, Tennessee 53273 sity, Nashville, Tennessee 53273
ix
X CONTRIBUTORS TO VOLUME 150
LEAHCIM-SNAH HCSOD (17), Department of IRVING REDIENHCSDLOG (31), Department
Paediatrics, Division of Immunology/ of Pathology, School of Medicine, Uni-
Rheumatology, The Hospital For Sick versity of Connecticut Health Center,
Children, Toronto, Ontario, Canada M5G Farmington, Connecticut 23060
1X8 PHILLIP GORDEN (53), National Institute of
Diabetes, and Digestive and Kidney Dis-
MEHER M. ROOTSUD (4), Department of Ar-
eases, National Institutes of Health, Be-
tificial Organs, The Research Institute,
thesda, Maryland 29802
Cleveland Clinic Foundation. Cleveland,
Ohio 44106 YRRAL M. NODROG (35), Biochemical Divi-
sion, Rees-Stealy Research Foundation,
C. GARRISON FATHMAN (24, 27, 28), De-
San Diego, California 10129
partment of Medicine, Division of Immu-
nology, Stanford University Medical Cen- GROJ YZNOROG (28), Department of Medi-
ter, Stanford, California 50349 cine, Division of Immunology, Stanford
University Medical Center, Stanford,
SALGUOD T. FEARON (46), Department of
California 50349
Medicine, Division of Molecular and Clin-
RENRAW C. GREENE (52), Howard Hughes
ical Rheumatology, The Johns Hopkins
Medical Institute, Department of lnternal
University School of Medicine, Balti-
Medicine, Duke University School of
more, Maryland 50212
Medicine, Durham, North Carolina 27710
Ross D. FELDMAN (39), Department of In-
EGROEG REGREBNURG (53), Diabetes Pro-
ternal Medicine and Pharmacology, Uni-
gram, Wayne State School of Medicine,
versity of lowa College of Medicine,
Detroit, Michigan 10284
Ames, Iowa 52242
JANICE M. HALL (1), Department of Molec-
ARABRAB G. REHCSORF (14), Department of ular Biology, Vanderbilt University,
Immunology, Scripps Clinic and Re- Nashville, Tennessee 53273
search Foundation, La Julia, California
JOHN A. HANSEN (43), Histocompatibility
73029 Laboratory, Puget Sound Blood Center,
TREBOR M. HTIARBLAG (32), Department of and Fred Hutchinson Cancer Research
Basic and Clinical Immunology and Mi- Center, Department of Medicine, Univer-
crobiology, Medical University of South sity of Washington, Seattle, Washington
Carolina, Charleston, South Carolina 40189
52492 JOSE A. HEDO (53), Departmento de Meta-
bolismo, Nutrici6n y Hormonas, Fiunda-
RAIF S. GEHA (25), Division of Immunol-
ci6n Jimenez Diaz, 2 Madrid 28040, Spain
ogy, Children's Hospital, Boston, Massa-
chusetts 02115 YERFFOEG J. HOWLETT (36), Russell
Grimwade School of Biochemistry, Uni-
ERWIN W. GELFAND (17), National Jewish
versity of Melbourne, Parkville, Victoria
Center for Immunology and Respiratory
3052, Australia
Medicine, Denver, Colorado 60208
R. HUEY (49), Teacher Education Program,
LIRREM J. NETSREG (10), Developmental Bi- University of California--San Diego, La
ology Laboratory, The Salk Institute for Julia, California 39029
Biological Studies, La Julia, California
T. E. HUGLI (49), Department of Immunol-
73029
ogy, Scripps Clinic and Research Foun-
ENAI-IREB GHEBREHIWET (45), Department dation, La Julia, California 92037
of Medicine, State University of New IKIHSOT ISHIHARA (24), Division of Immu-
York, Stony Brook, New York 1618-49711 nology, Department of Medicine, Stan-
DNALOR H. GISLER (19), Basel Institute for ford University Medical Center, Stanford,
Immunology, CH-4005 Basel, Switzerland California 50349
CONTRIBUTORS TO VOLUME 150 xi
JUDITH P. JOHNSON (30), Institute for Im- Carolina, Charleston, South Carolina
munology, University of Munich, D-8000 52492
Munich 2, Federal Republic of Germany ECINREB SLEUMAS-SUCRAM (53), Diabetes
TTENRAG KELSOE (23), Department of Mi- Branch, National Institute of Diabetes,
crobiology, University of Texas Medical Digestive and Kidney Diseases, National
Branch, Galveston, Texas 05577 Institutes of Health, Bethesda, Maryland
29802
H. KIRCHNER (8), Institute of Virus Re-
search, German Cancer Research Center, PAUL J. MARTIN (43), Department of Medi-
6900 Heidelberg, Federal Republic of cine, University of Washington, Seattle,
Germany Washington 40189
DAGMAR KLEIN (13), Abteilung lm- Louis A. MATIS (29), Molecular Immunol-
mungenetik, Max-Planck-lnstitut far ogy Laboratory, Division of Biochemistry
Biologie, 7400 Tiibingen, Federal Repub- and Biophysics, Center for Drugs and Bi-
ologics, Food and Drug Administration,
lic of Germany
Bethesda, Maryland 29802
JAN KLEIN (13), Abteilung lmmungenetik,
NEELHTAK SHIVE SWEHTTAM 1( !), Depart-
Max-Planck-lnstitut far Biologie, 7400
ment of Biochemistry, Rice University,
Tiibingen, Federal Republic of Germany
Houston, Texas 15277
NAMRON R. KLINMAN (14), Department of
EUGENE S. KCOLDEM (31), Department of
Immunology, Research Institute of
Exploratory Research, AMGEN Corpora-
Scripps Clinic, La Jolla, California 92037
tion, Thousand Oaks, California 02319
WILLIAM T. LEE (40), Department of Mi-
FmTZ SREHCLEM (16), Basel Institute for
crobiology, University of Texas Health
Immunology, CH-4058 Basel, Switzerland
Science Center, Dallas, Texas 53257
C. D. SREYM (26), Department of Microbiol-
NAVI STIVOKFEL (15, 18), Basel Institute for ogy, University of Texas Health Science
Immunology, CH-4005 Basel, Switzerland Center at Dallas, Dallas, Texas 53257
NERRAW J. LEONARD (52), Cell Biology and YRRAB L. SENOYM (47), Division of Rheu-
Metabolism Branch, National Institute of matology/Immunology, Departments of
Child Health and Human Development, Medicine and Pediatrics, University of
National Institutes of Health, Bethesda, North Carolina, Chapel Hill, North Caro-
Maryland 29802 lina 27514
ENIXAM A. LESNIAK (53), Diabetes Branch, GLEN R. WOREMEN (44), Scripps Clinic and
National Institute of Diabetes, Digestive Research Foundation, Department of lm-
and Kidney Diseases, NatMnal Institutes munology, La Julia, California 92037
of Health, Bethesda, Maryland 29802 DNALOR A. NEWMAN (54), Cancer Center,
ARDNAXELA LIVINGSTONE (27), Depart- University of California--San Diego, La
ment of Medicine, Division of Immunol- Julia, California 39029
ogy, Stanford University Medical Center, KENJI OGATA (12), Scripps Clinic and Re-
Stanford, California 50349 search Foundation, La Julia, California
FRANK D. LOONEY (35), Division of Bio- 73029
technology, CSIRO, Clayton, Victoria K. G. OLIVER (26), Department of Microbi-
3168, Australia ology, University of Texas Health Science
R. JOHN YENOOL (42), Department of Medi- Center at Dallas, Dallas, Texas 53257
cine, University of Rochester Medical IKAIHSOT OSAWA (2), Division of Chemical
Center, Rochester, New York 24641 Toxicology and Immunochemistry, Fac-
JOHN J. SINOLAHCRAM (32, 34, 37, 50), De- ulty of Pharmaceutical Sciences, Univer-
partment of Biochemistry and Molecular sity of Tokyo, Bunkyo-ku, Tokyo 113,
Biology, Medical University of South Japan
xii CONTRIBUTORS TO VOLUME 150
REHPOTSIRHC J. PAIGE (19, 20), Basel Insti- ATIRAGRAM SALAS (8), Department of Bio-
tute for Immunology, CH-4005 Basel, chemistry, School of Medicine, Avenida
Switzerland de Madrid, 18012 Granada, Spain
SELRAHC W. REKRAP (3, 41), Division of Al- V. M. SREDNAS (26), Department of Micro-
lergy and Immunology, Department of biology, University of Texas Health Sci-
Medicine, Howard Hughes Medical Insti- ence Center at Dallas, Dallas, Texas
tute at Washington University School of 53257
Medicine, St. Louis, Missouri 01136 HELMUT SAUTER (20), Basel Institute for
AROLF PETTIT (11), Clayton Foundaton Bio- Immunology, CH-4005 Basel, Switzerland
chemical Institute, Austin, Texas -21787 LEUMAS F. SCHLUTER (50), Department of
6901 Biochemistry and Molecular Biology,
Medical University of South Carolina,
MARK W. PIERCE (48), Harvard Medical
Charleston, South Carolina 52492
School, Massachusetts General Hospital,
and The Howard Hughes Institute, Bos- DAVID M. SEGAL (38), Immunology Branch,
ton, Massachusetts 02114 National Cancer Institute, National Insti-
tutes of Health, Bethesda, Maryland
YELREVEB L. PIKE (21), The Walter and
29802
Eliza Hall Institute of Medical Research,
Royal Melbourne Hospital, Victoria 3050, WILLIAM SHIVE (11), Department of Chem-
Australia istry and Clayton Foundation Biochem-
ical Institute, The University of Texas at
SAMOHT NNAMSTROP (9), Institute of Medi-
Austin, Austin, Texas 6901-21787
cal Immunology, Faculty of Medicine
SEMAJ V. SORATS (40), Department of Bio-
(Charitd), Humbold University of Berlin,
chemistry, Vanderbilt University, School
1040 Berlin, German Democratic Re-
of Medicine, Nashville, Tennessee 23273
public
DAVID A. STEPHANY (38), Biological Re-
SABBA RASHIDBAIGI (39), Department of
search Branch, National Institute of Al-
Molecular Genetics and Microbiology,
lergy and Infectious Disease, Bethesda,
University of Medicine and Dentistry of
Maryland 20892
New Jersey, Piscatuway, New Jersey
45880 ENG M. NAT (12), Scripps Clinic and Re-
search Foundation, La Jolla, California
NEVETS A. GREBNESOR (29), Surgical On-
73029
cology Branch, National Cancer Insti-
tute, National Institutes of Health, Be- THI~I~SE KCNYNRET (9), UniN d'Immuno-
thesda, Maryland 20892 cytochimie, D#partement d'lmmunolo-
gie, Institut Pasteur, 75724 Paris Cedex
NODROG D. Ross (47), Division of Rheuma-
,51 France
tology/lmmunology, Departments of
Medicine and Microbiology~Immunology, LUANN NOSPMOHT (1), Department of Mo-
University of North Carolina, Chapel lecular Biology, Vanderbilt University,
Hill, North Carolina 27514 Nashville, Tennessee 53273
ESSEJ ROTH (53), National Institute of Dia- JULIE A. TITUS (38), Immunology Branch,
betes, Digestive and Kidney Diseases, National Cancer Institute, National Insti-
National Institutes of Health, Bethesda, tutes of Health, Bethesda, Maryland
Maryland 29802 29802
ARNOLD E. RUOHO (39), Department of J. W. UHR (26), Department of Microbiol-
Pharmacology, University of Wisconsin ogy, University of Texas Health Science
Medical School, Madison, Wisconsin Center at Dallas, Dallas, Texas 53257
60735 DALE T. UMETSU (25), Division of Immu-
CONTRIBUTORS TO VOLUME 150 xiii
nology, Children's Hospital, Boston, C. A. WmTLOCK (22), Department of Pa-
Massachusetts 02115 thology, Stanford University, Stanford,
SAMOHT L. VISCHER (7), Division of Rheu- California 50349
matology, Department of Medicine, Uni- O. N. WITTE (22), Molecular Biology Insti-
versity Hospital, CHoI211 Geneva, Switz- tute, University of California, Los
erland Angeles, California 90024
E. S. VITETTA (26), Department of Microbi- WlNNIE W. WONG (46), Department of
ology, University of Texas Health Science Rheumatology and Immunology,
Center at Dallas, Dallas, Texas 53257 Brigham & Women's Hospital, and De-
YROGERG W. WARS (34), Department of partment of Rheumatology and Immunol-
Biochemistry and Molecular Biology, ogy, Harvard Medical School, Boston,
Medical University of South Carolina, Massachusetts 51120
Charleston, South Carolina 29425 GILLIAN E. Wu (20), Basel Institute for Im-
CORNELIA WEYAND (28), Department of munology, CH-4005 Basel, Switzerland
Medicine, Division of Immunology, Stan- E. YEFENOF (26), Department oflmmunol-
ford University Medical Center, Stanford, ogy, Hadassah Medical School, Jerusa-
California 50349 lem 91010, Israel
Preface
This is the last of a set of volumes in the Immunochemical Techniques
series of Methods in Enzymology dealing with methods for the study of
the biology of lymphoid cells. The other two volumes (108 and )611 dealt
with the separation and characterization of lymphoid cells and with the
effectors and mediators of lymphoid cell functions, respectively. In this
volume in vitro models for the study of lymphoid cell functions and meth-
ods for the study of lymphoid cell receptors are presented.
The area of lymphocyte in vitro transformation has received consider-
able attention in recent years. This subject is discussed in the first section
that describes methods for the in vitro stimulation of lymphocytes. Some
special media for the study of lymphocyte transformation are also dis-
cussed.
In vitro methods have been very useful for the understanding of the
differentiation of lymphoid cells. The more commonly used of these meth-
ods are presented in the second section.
Finally, the third section deals with the receptors of lymphoid ceils.
This area of immunology has developed dramatically recently. It would
have been difficult to include the methods for the study of all the known
receptors of lymphoid cells. Thus, only those receptors that are charac-
teristic of these cells and/or are specifically involved in some functions of
lymphoid cells have been discussed.
I acknowledge the help and advice of the late Drs. Sidney Colowick
and Nathan Kaplan in the organization and preparation of this volume of
Immunochemical Techniques.
INNAVOIG DI OTABAS
XV
METHODS IN ENZYMOLOGY
EDITED BY
Sidney P. Colowick and Nathan O. Kaplan
VANDERBILT UNIVERSITY DEPARTMENT OF CHEMISTRY
SCHOOL OF MEDICINE UNIVERSITY OF CALIFORNIA
NASHVILLE, TENNESSEE AT SAN DIEGO
LA JOLLA, CALIFORNIA
I. Preparation and Assay of Enzymes
II. Preparation and Assay of Enzymes
III. Preparation and Assay of Substrates
IV. Special Techniques for the Enzymologist
.V Preparation and Assay of Enzymes
VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
VII. Cumulative Subject Index
xvii
METHODS IN ENZYMOLOGY
EDITORS-IN-CHIEF
Sidney P. Colowick and Nathan O. Kaplan
EMULOV VIII. Complex Carbohydrates
Edited by ELIZABETH F. NEUFELD DNA VICTOR GRUBSNIG
EMULOV IX. etardyhobraC Metabolism
Edited by WILLIS A. DOOW
EMULOV X. Oxidation and Phosphorylation
Edited by RONALD W. KOORBATSE DNA DRANYAM E. NAMLLUP
EMULOV XI. Enzyme Structure
Edited by C. H. W. HIRS
EMULOV XII. Nucleic Acids (Parts A and B)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XIII. Citric Acid Cycle
Edited by J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited by J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited by DNOMYAR I. NOTYALC
EMULOV XVI. Fast Reactions
Edited by HTENNEK NITSUK
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited by TREBREH ROBAT DNA CELIA WHITE ROBAT
xix
XX METHODS IN ENZYMOLOGY
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited by DLANOD B. KCIMROCCM DNA LEUMEL D. THGIRW
EMULOV XIX. Proteolytic Enzymes
Edited by EDURTREG E. NNAMLREP DNA OLZSAL DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited by MAILLIW B. YBOKAJ
EMULOV XXlII. Photosynthesis (Part A)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part )A
Edited by SIDNEY FLEISCHER AND LESTER PACKER
xxi
METHODS IN ENZYMOLOGY
EMULOV XXXII. Biomembranes (Part B)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by AHTRAM G. SINNED DNA DRAWDE A. SINNED
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited by MAILLIW B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XXXV. Lipids (Part B)
Edited by JOHN M. NIETSNEWOL
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited by JOEL G. NAMDRAH DNA BERT W. YELLAM'O
EMULOV XXXlX. Hormone Action (Part D: Isolated Cells, Tissues, and
Organ Systems)
Edited by JOEL G. NAMDRAH DNA BERT W. YELLAM'O
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited by W. A. DOOW
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited by W. A. DOOW
EMULOV XLIII. Antibiotics
Edited by JOHN H. HSAH
EMULOV XLIV. Immobilized Enzymes
Edited by KLAUS HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part )B
Edited by OLZSAL DNAROL
