Table Of ContentFUNCTION AND METABOLISM
OF PHOSPHOLIPIDS
IN THE
CENTRAL AND PERIPHERAL
NERVOUS SYSTEMS
ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY
Editorial Board:
Nathan Back State University of New York at Buffalo
N. R. Di Luzio Tulane University School of Medicine
Bernard Halpern College de France and Institute of Immuno·Biology
Ephraim Katchalski The Weizmann Institute of Science
David Kritchevsky Wistar Institute
Abel Lajtha New York State Research Institute for Neurochemistry and Drug Addiction
RodoIro Paoletti University 0/ Milan
Recent Volumes in this Series
Volume 64
IMMUNOLOGIC PHYLOGENY
Edited by W. H. Hildemann and A. A. Benedict· 1975
Volume 65
DILEMMAS IN DIABETES
Edi ted by Stewart Wolf and Beatrice Bishop BerIe • 1975
Volume 66
IMMUNE REACTIVITY OF LYMPHOCYTES: Development, Expression,
and Control
Edited by Michael Feldman and Amiela Globerson • 1976
Volume 67
ATHEROSCLEROSIS DRUG DISCOVERY
Edited by Charles E. Day. 1976
Volume 68
CURRENT TRENDS IN SPHINGOLIPIDOSES AND ALLIED DISORDERS
Edited by Bruno W. Volk and Larry Schneck. 1976
Volume 69
TRANSPORT PHENOMENA IN THE NERVOUS SYSTEM: Physiological and
Pathological Aspects
Edited by Giulio Levi, Leontino Battistin, and Abel Lajtha • 1976
Volume iO
KININS: Pharmacodynamics and Biolo/l:ical Roles
Edited hy F. Sicuteri, Nathan Back, and G. L. Haberland. 1976
Volume 71
GANGLIOSIDE FUNCTION: Biochemical and Pharmacological Implications
Edited by Giuseppe Porcellati, Bruno Ceccarelli, and Guido Tettamanti • 1976
Volume 72
FUNCTION AND METABOLISM OF PHOSPHOLIPIDS IN THE CENTRAL
AND PERIPHERAL NERVOUS SYSTEMS
Edited by Giuseppe Porcellati, Luigi Amaducci, and Claudio Galli. 1976
FUNCTION AND METABOLISM
OF PHOSPHOLIPIDS
IN THE
CENTRAL AND PERIPHERAL
NERVOUS SYSTEMS
Edited by
Giuseppe Porcellati
University of Perugia
Perugia, Italy
Luigi Amaducci
University of Florence
Florence, Italy
and
Claudio Galli
University of Milan
Milan, Italy
PLENUM PRESS • NEW YORK AND LONDON
Library of Congress Cataloging in Publication Data
International Satellite Meeting on Function and Metabolism of Phospholipids in
Central and Peripheral Nervous Systems, Cortona, 1975.
Function and metabolism of phospholipids in the central and peripheral nervous
systems.
(Advances in experimental medicine and biology; v. 72)
Includes index.
1. Neurochemistry-Congresses. 2. Phosphatides-Congresses.1. Porcellati, Giuseppe.
II. Amaducci, Luigi. III. Galli, Claudio. IV. Title. V. Series. [DNLM: 1. Phospholipids
Physiology-Congresses. 2. Phospholipids-Metabolism-Congresses. 3. Central nervous
system-Metabolism-Congresses. 4. Peripheral nerves-Metabolism-Congresses. WI
AD559 v.72 1975/QU93 I6lf 1975J
QP356.3.I52 1975 599'.01 '88 76·15617
ISBN 978-1-4684-0957-4 ISBN 978-1-4684-0955-0 (eBook)
DOl 10.1007/978-1-4684-0955-0
Proceedings of the International Satellite Meeting on
Function and Metabolism of Phospholipids in Central
and Peripheral Nervous Systems held at Cortona, Tuscany,
Italy, August 1975
©1976 Plenum Press, New York
Softcover reprint of the hardcover 1s t edition 1976
A Division of Plenum Publishing Corporation
227 West 17th Street, New York, N.Y. 10011
All rights reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted,
in any form or by any means, electronic, mechanical, photocopying, microfilming,
recording, or otherwise, without written permission from the Publisher
Preface
The present volume contains all the contributions and general
discussion presented at the International Satellite Meeting on Func
tion and Metabolism of Phospholipids in Central and Peripheral
Nervous Systems held at Cortona, Tuscany, Italy in August 1975.
The Satellite Meeting was organized on the frame of the 5th Inter
national Congress of the International Society for Neurochemistry
(Barcelona, 2-7 september 1975) and was just run before it. The
publication of the scientific content of this volume has been made
possible by the collaboration of the speakers, the discussants,
the Meeting Chairman, the section chairmen and of all the scientists
who have taken part at the Symposium and who deeply and actively
discussed the lectures and the contributions to the General Discus
sion which were delivered. In order to obtain rapid publication
of the volume, however, the single discussions for each delivered
contribution will not be reported here.
The general subject of membrane structure, of the turnover
of its lipid components in CNS and PNS, their functional implica
tions and pharmacological actions, was explored in details from
the stand-points of the various contributors in biophysics, biochem
istry, physiology, cytology, pharmacology and pathology. The
whole Symposium was effiCiently introduced and closed by Dr. W.
Stoffel.
The meeting has been thought to have been very successful.
It gave certainly in our opinion a noticeable stimulus to bioche
mical research workers in the field of lipid neurochemistry. The
editors express their thanks to the authors of the papers and to
the Plenum Publishing Company for the rapid response. which has ena
bled the prompt publication of this volume, and to the auditorium
v
PREFACE
of the meeting, which was attended by more than two hundred research
workers actively interested in problems of lipid neurochemistry.
Our final thank is for Dr. Francesco della Valle, official
representative of FIDIA Research Laboratories, Abano Terme, Padua,
Italy, to whom we are grateful for his continuous and efficient
support. The contribution of the Secretarial Staffs of both the
Department of Biochemistry of Perugia University and FIDIA Labo
ratories to the editorial work is also gratefully acknowledged.
Perugia, December 1975 Giuseppe Porcellati
Luigi Amaducci
Claudio Galli
Contents
STRUCTURAL REQUIREMENTS FOR PHOSPHOLIPID IN BIOLOGICAL MEMBRANES
Significance of Minor Glycerolipids in Membrane
Structure and Function .•.•.•..•....•.••••. 3
R.H. Michell, D. Allan and J.B. Finean
Effect of Phospholipase-C on the Structure and Function
of Mitochondria and Sonic Fragments .••••.. 15
G.F. Azzone, E. Viola, E. Strinna and M. Bragadin
Contribution of Phospholipids to the Surface Charge of
Neuronal Membranes 21
W.J. Moore
METABOLISM AND TURNOVER OF PHOSPHOLIPIDS IN NERVOUS TISSUE
Cytidine Diphosphate Diglyceride of Bovine Brain and
Liver: Isolation and Characterization •••.• 27
W. Thompson
Synthesis and Turnover of Brain Phosphoglycerides
Results, Methods of Calculation
and Interpretation .••••.•••••••••••••••••• 37
L.A. Horrocks, A.D. Toews, D.K. Thompson and
J.Y. Chin
vii
viii CONTENTS
Metabolic Studies on 1-Alkyl- and 1-Alk-1'-Enyl
Glycero-phosphatides in the Brain 55
H. Woelk, G. Porcellati and G. Goracci
The Contribution of Net Synthesis and Base-Exchange
Reaction in Phospholipid Biosynthe-
63
S1S ••••••••••••••••••••••••••••••••••••••
G. Arienti, M. Brunetti, A. Gaiti, p. Orlando
and G. Porcellati
Regulatory Aspects of Phospholipid Metabolism in
Brain ..................................... 79
P. Orlando, F. Cerrito and G. Porcellati
Renewal of Phospholipids in the Myelin Sheath 95
R.M.Q Dawson anu R.M. Gould
Studies on Phospholipid Metabolism in Cultured
Dissociated Brain Cells •••••••••••••••••• 115
E. Yavin
The Turnover of Choline and Ethanolamine Plasmalogens
in Glial and Neuronal Cells of the
Rabbit in vivo 123
G. Goracci, E. Francescangeli, R. Mozzi,
H. Woelk and G. Porcellati
Phospho- and Glycosphingolipid Metabolism in a Rat Neurinoma
Clone (RN6) Studied in Tissue
CuI ture .................................. 131
L. Binaglia, K. Karageosian and W. Stoffel
De Novo Glycerolipid Biosynthesis in the Toad and
Cattle Retina. Redirecting of the
Pathway by Propranolol and Phentol-
am1lle ....................•...........•... 139
N. G. Bazan, M.G. llincheta de Boschero, N.M.
Giusto and H.E. Pascual de Baz~
Accumulation and Metabolism of Phosphatidyl-CMP (CDP
Diglyceride) in the Pineal Gland
of the Rat •••••.••••.•••••••••••••••••••• 149
G. Hauser and J. Eichberg
CONTENTS ix
ASPECTS OF PHOSPHOLIPID FUNCTION IN THE NERVOUS SYSTEM
The Role of Choline Kinase in the Brain ••••••••••••••••••••• 161
G.B. Ansell and S. Spanner
Functional Roles of Phospholipids of Synaptosomal
Membrane .••••.•••••••...••.•.••..••..•••• 169
A. Y. Sun and G. Y. Sun
Phospholipid Metabolism in Electrically Stimulated
Synaptosomes ••••••••••••••••••••••••••••• 199
J.N. Hawthorne, J.E. Bleasdale and M.R.
Pickard
The Effects of Neurotransmitters and Cyclic Nucleotides
on the Metabolism of Phospholipids in
Synaptic Membranes of Brain •••••••••••••• 211
C.E. Rowe and R.J. Gullis
Effects of Neurotransmitters and Neuropharmacological
Agents on Phospholipid Metabolism
in the Rabbit Iris Muscle ••••••••.••••••• 227
A.A. Abdel-Latif
Phosphatidylinositol Metabolism and Myoinositol
1,2-Cyclic Phosphate Action in
Smooth Muscle ••••••••••••.••••••••••••••• 257
E.G. Lapetina and L.M. Zieher
Phospholipids and the Gaba Receptor ••••••.••.•...•.....••••• 265
C.T. Giambalvo and p. Rosenberg
PHARMACOLOGICAL ACTIONS OF PHOSPHOLIPIDS ON THE NERVOUS SYSTEM
Effect of Polar Lipids on Cerebral Content of
Free Glucose in Mice ••••••••••••••••••••• 271
A. Bruni, A. Leon and E. Boarato
x CONTENTS
Investigations into the Relationship between
Phospholipids and Brain Acety1
choline ................................. 285
p. Mantovani, G. Pepeu and L. Amaducci
Phospholipid-Induced Modifications in the Brain
of Animals Fed with Cholesterol
Supplemented Diet •••••.•••••••••••••.••• 293
G. Toffano, p. Gonzato, and D. Benvegnu
Possible Role of BC-PL in Enhancing 32pi Incorporation
into Mice Brain Phospholipids ••••••••••• 307
A. Leon and G. Toffano
PHOSPHOLIPID IN BRAIN DAMAGE
Free Arachidonic Acid and Other Lipids in the Nervous
System during Early Ischemia and
after Electroshock •••••••••••••••.••••••• 317
N.G. Bazan
Dietary Induced Modifications of Brain Polyunsaturated
Fatty Acids and Changes of Free
Arachidonic Acid Release during
Ischaemia •••••.••••••••••••••••••••••••• 337
C. Galli, C. Spagnuolo, M. Rodriguez and
R.J. Cenedella
Possible Effects of Serum Transferases on Brain
Phospholipid Metabolism ••••••••••••••••• 347
D. Inzitari, L. Bartolini, G.E. De Medio and
L. Amaducci
Modifications of Glycerol Phosphoglyceride Metabolism
in Cell Storage Phenomena ••••••••••••••• 357
J.R. Wherret and S. Huterer
