Table Of ContentControl of Transcription
BASIC LIFE SCIENCES
Alexander Hollaender, General Editor
Biology Division
Oak Ridge National Laboratory
and The University of Tennessee
Knoxville
1973: Volume 1 • GENE EXPRESSION AND ITS REGULATION
Edited by F. T. Kenney, B. A. Hamkalo. G. Favelukes.
and J. T. August
Volume 2 • GENES. ENZYMES, AND POPULATIONS
Edited by A. M. Srb
1974: Volume 3 • CONTROL OF TRANSCRIPTION
Edited by B. B. Biswas. R. K. Mandai, A. Stevens,
and W. E. Cohn
In preparation:
• REPRODUCTIVE PHYSIOLOGY AND GENETICS
Edited by F. Fuchs and E. M. Coutinho
A Continuation Order Plan is available for this series. A continuation order
will bring delivery of each new volume immediately upon publication. Volumes
are billed only upon actual shipment. For further information please contact
the publisher.
Control of
Transcription
Edited by
B. B. Biswas and R. K. Mandai
Bose Institute
Calcutta, India
and
A. Stevens and W E. Cohn
Biology Division
Oak Ridge National Laboratory
Oak Ridge, Tennessee
PLENUM PRESS · NEW YORK- LONDON
Library of Congress Cataloging in Publication Data
Calcutta Symposium on Control of Transcription, 1973.
Control of transcription; [proceedings]
(Basic life sciences, v.3)
Includes bibliographical references.
1. Genetic transcription-Congresses. I. Biswas, B. B., ed. II. Title. [DNLM: 1.
Genetics, Microbial-Congresses. 2. Transcription, Genetic-Congresses. QH431 C144c
1973]
QH450.2.C34 1973 575.2'1 73-20166
ISBN-13: 978-1-4613-4531-2 e-ISBN-13: 978-1-4613-4529-9
001: 10.1007/978-1-4613-4529-9
Proceedings of the Calcutta Symposium on Control of Transcription,
February 12-15, 1973
© 1974 Plenum Press, New York
Softcover reprint of the hardcover 15t edition 1974
A Division of Plenum Publishing Corporation
227 West 17th Street, New York, N.Y. 10011
United Kingdom edition published by Plenum Press, London
A Division of Plenum Publishing Company, Ltd.
Davis House (4th Floor), 8 Scrubs Lane, Harlesden, London, NW10 6SE, England
All rights reserved
No part of this publication may be reproduced in any form without
written permission from the publisher
ORGANIZING COMMITTEE
Alexander Hol/aender-Oak Ridge National Laboratory, Oak Ridge, Tennessee,
U.S.A., and University of Tennessee, Knoxville, Tennessee, U.S.A.
Sankar Mitra-Oak Ridge National Laboratory, Oak Ridge, Tennessee, U.S.A.
Salil K. Niyogi-Oak Ridge National Laboratory, Oak Ridge, Tennessee, U.S.A.
S. M. Sircar-Bose Institute, Calcutta, India.
R. K. Mandai-Bose Institute, Calcutta, India.
S. Ghosh-Bose Institute, Calcutta, India.
B. B. Biswas, Convener-Bose Institute, Calcutta, India.
SPONSORS
Bose Institute, Calcutta 700009, India.
Oak Ridge National Laboratory, Biology Division, Oak
Ridge, Tennessee 37830, U.S.A.
United States National Science Foundation.
Ministry of Science and Technology, Government of India.
ACKNOWLEDGMENTS
The following institutions and firms have extended their generous collaboration
and assistance:
Rabindra Bharati University, Calcutta.
United States Consulate, Calcutta.
Toshniwal Brothers Pvt. Ltd., Calcutta.
Systronics, Calcutta.
Borosil Glass Works Ltd .. Calcutta.
Foreword
In numerous conversations with our colleagues from India, it was suggested
that we help to institute a series of symposia in India similar in nature to those
that have been conducted by our Latin American colleagues for more than 10
years. We were fortunate to have with us in Oak Ridge Dr. Niyogi and Dr. Mitra
from Indian universities. Their close ties with the Bose Institute in Calcutta and
the resultant correspondence with the Institute Director, Dr. S. M. Sircar,
provided the stimulus for organization of this first Indian symposium, which was
held in Calcutta. Under the direction of Dr. Sircar, Dr. B. B. Biswas did an
outstanding job of organizing this conference.
Financial support was arranged through Dr. R. R. Ronkin of the United
States National Science Foundation, who smoothed the way for the use of PL
480 funds which were approved by the Indian Government for the organization
and running of this most valuable symposium.
The many Indian scientists who contributed papers and enthusiastically and
vigorously entered into the discussions demonstrated the strength of modern
science in India. The topic, Control of Transcription, is a timely one, and
considerable activity in this area is going on all over the world. The success of
this symposium speaks well for the future of these Indian conferences and
workshops being planned for the next few years.
Again, the worldwide "community of science" is clearly manifested by the
close cooperation we have observed in this fruitful and successful symposium.
Alexander Hollaender
Biology Division
Oak Ridge National Laboratory
Oak Ridge, Tennessee
and
The University of Tennessee
Knoxville, Tennessee
vii
Contents
Introduction
B. B. Biswas xiii
List of Participants xv
I. Fine Structure of Active Genes in Prokaryotes and Eukaryotes
Barbara A. Hamkalo and O. L. Miller, Jr 1
2. Changes in the Subunit Structures of Bacillus subtilis RNA Poly-
merase During Sporulation
Richard Losick 15
3. Control of Transcription in Bacillus brevis by Small Molecules
Henry Paulus and Nilima Sarkar 21
4. Control of Transcription in Phage P22 Infected Host
Maharani Chakravorty, P. S. Khandekar,
G. R. Koteswara Rao, and Sushil Taneja .35
5. New Small Proteins Associated with DNA-Dependent RNA Poly-
merase of Escherichia coli After Infection with T4 Phage
Audrey Stevens and R. Diane Crowder 53
6. The Stringent Response-21 Years On
Andrew Travers 67
7. Elucidation of RNA Initiation (DNA Promoter?) Sequences in T4
DNA Transcription using Escherichia coli RNA Polymerase and
Dinudeoside Monophosphates
Salil K. Niyogi and David J. Hoffman 81
ix
x Contents
8. Stringent Coupling Between Transcription, Translation, and
Degradation of Messenger RNA in Inducible Enzyme Systems: A
Theoretical Analysis
U. N. Singh 93
9. Gene-Specific Changes in Induced Enzyme Synthesis in Escherichia
coli Infected with Phage </>X 174
Amit Ghosh and Ramendra K. Poddar 105
10. Initiation of Transcription by RNA Polymerases of Escherichia coli
and Phage T3
E. K. F. Bautz, W. T. McAllister, H. Kilpper,
E. Beck, and F. A. Bautz 115
11. Transcription of Native and Denatured DNA Preparations by Bac-
teriophage T3 Induced RNA Polymerase
Umadas Maitra, Prasanta R. Chakraborty, Rino A. Salvo,
Henry H. Huang, Pradip Bandyopadhyay, and Probir Sarkar 125
12. Physical Mapping of Transcribing Regions on Coliphage fd DNA by
the Use of Restriction Endonucleases
M. Takanami and T. Okamoto 145
13. Transcriptional Control of the Expression of a Degradative Plasmid
in Pseudomonas
A. M. Chakrabarty 157
14. Studies on the Transcription of Simian Virus 40 and Adenovirus
Type 2
Joe Sambrook, Phillip A. Sharp, Brad Ozanne,
and Ulf Pettersson .167
15. Transcription of Reovirus RNA
Amiya K. Banerjee, C. Martin Stoltzfus, Richard L. Ward,
and Aaron J. Shatkin 181
16. Initiation and Regulation of Transcription in Coliphage A
Waclaw Szybalski 201
17. Termination and Antitermination in Transcription: Control of Gene
Expression
Sankar Adhya, Max Gottesman, and Benoit de Crombrugghe 213
18. Control of Transcription in Neurospora crassa
P. R. Mahadevan and A. S. Bhagwat 223
19. Transcriptional Systems in Eukaryotic Cells
Michael Goldberg, Jean-Claude Perriard, Gordon Hager,
Richard B. Hallick, and William J. Rutter 241
Contents xi
20. Transcription of Double-Stranded Viral and Cellular DNAs by
Purified Mammalian DNA-Dependent RNA Polymerases
P. Chambon, J. L. Mandel, F. Gissinger, C. Kedinger,
M. Gross-Bellard, and P. Hossenlopp 257
21. Factors Affecting the Selection of a Template for the Characteriza-
tion of Multiple DNA-Dependent RNA Polymerases of Mammalian
Tissues
Peter H. W. Butterworth, Sarah Jane Flint,
and C. James Chesterton 269
22. RNA Polymerases and Controlling Factors from Plant Cell Nuclei
B. B. Biswas, H. Mondal, A. Ganguly,
Asis Das, and R. K. Mandai 279
23. Restriction of RNA Synthesis by RNA Polymerase from Avian
Erythrocyte Nuclei
R. K. Mandai, Hemanta K. Mazumder, and B. B. Biswas 295
24. On the Regulation of Pre-mRNA Biosynthesis and Transport
E. M. Lukanidin, O. P. Samarina, A. P. Ryskov,
and G. P. Georgiev .303
25. The Poly(A) Sequences in Messenger RNA and Heterogeneous
Nuclear RNA
Hiroshi Nakazato and Mary Edmonds 317
26. Estradiol-Induced Expression of Genetic Information: Synthesis of
Phosvitin in Birds
G. P. Talwar, B. L. Jailkhani, M. L. Sopori, S. Venkatesan,
A. Grover, P. R. Narayanan, and C. Narasimhan .333
27. Comparison of Biochemical Characteristics of Reverse Transcriptase
from Human Acute Leukemic Cells and Several RNA Tumor
Viruses
P. S. Sarin, J. W. Abrell, and R. C. Gallo .345
28. Synthesis by Reverse Transcriptase of DNA Complementary to
Globin Messenger RNA
lnder M. Verma, Gary F. Temple, Hung Fan,
and David Baltimore .355
29. Investigations on Reverse-Transcribed DNA from RNA Templates
M. R. Das .373
30. Transcriptional Control of M13 Phage DNA Replication
Sankar Mitra 383
xii Contents
31. Conversion of </>X174 and fd DNA to Their Replicative Forms by
Two Enzyme Systems in Escherichia coli
S. Wickner, R. B. Wickner, M. Wright, I. Berkower,
and J. Hurwitz .395
32. A Quantitative Estimate of DNA· RNA Hybridization in Neuro-
spora crassa
S. K. Dutta and D. R. Jagannath .411
Index 417
