Cell Biology, Four-Volume Set, Third Edition: A Laboratory Handbook

1899 Pages·2005·239.79 MB·English

by  Julio E. Celis

Preface
Scientific progress often takes place when new technologies. Appendices include representative
technologies are developed, or when old procedures cultured cell lines and their characteristics, Internet
are improved. Today, more than ever, we are in need resources in cell biology, and bioinformatic resources
of complementary technology platforms to tackle for in silico proteome analysis. The Handbook pro-
complex biological problems, as we are rapidly vides in a single source most of the classical and
moving from the analysis of single molecules to the emerging technologies that are essential for research in
study of multifaceted biological problems. The third the life sciences. Short of having an expert at your side,
edition of Cell Biology: A Laboratory Handbook brings the protocols enable researchers at all stages of their
together 236 articles covering novel techniques and career to embark on the study of biological problems
procedures in cell and molecular biology, proteomics, using a variety of technologies and model systems.
genomics, and functional genomics. It contains 561 Techniques are presented in a friendly, step-by-step
new articles, many of which were commissioned in fashion, and gives useful tips as to potential pitfalls of
response to the extraordinary feedback we received the methodology.
from the scientific community at large. I would like to extend my gratitude to the Associ-
As in the case of the second edition, the Handbook ate Editors for their hard work, support, and vision in
has been divided in four volumes. The first volume selecting new techniques. I would also like to thank the
covers tissue culture and associated techniques, staff at Elsevier for their constant support and dedica-
viruses, antibodies, and immunohistochemistry. tion to the project. Many people participated in the
Volume 2 covers organelles and cellular structures as realization of the Handbook and I would like to thank
well as assays in cell biology. Volume 3 includes in particular Lisa Tickner, Karen Dempsey, Angela
imaging techniques, electron microscopy, scanning Dooley, and Tari Paschall for coordinating and organ-
probe and scanning electron microscopy, micro- izing the preparation of the volumes. My gratitude is
dissection, tissue arrays, cytogenetics and in situ also extended to all the authors for the time and energy
hybridization, genomics, transgenic, knockouts, and they dedicated to the project.
knockdown methods. The last volume includes trans-
fer of macromolecules, expression systems, and gene Julio .E Celis
expression profiling in addition to various proteomic Editor
xlv
Contributors
Numbers in parenthesis indicate the volume (bold face) and page on which the authors' contribution begins.
Mads Aaboe :4( )38 Clinical Biochemical Terence Allen (3: 325) CRC Structural Cell Biology
Department, Molecular Diagnostic Laboratory, Group, Paterson Institute for Cancer Research,
Aarhus University Hospital, Skejby, Christie Hospital NHS Trust, Wilmslow Road,
Brendstrupgaardvej, Aarhus N, DK-8200, Withington, Manchester, M20 4BX, UNITED
DENMARK KINGDOM
Tanja Aarvak :1( 239) Dynal Biotech ASA, PO Box Noona Ambartsumian :1( 363) Department of
,411 Smestad, N-0309, NORWAY Molecular Cancer Biology, Danish Cancer Society,
Institute of Cancer Biology, Strandboulevarden 49,
Harindra R. Abeysinghe :3( 345) Department of
Copenhagen, DK-2100, DENMARK
Pathology and Laboratory Medicine, University of
Rochester School of Medicine, 106 Elmwood Ave., Oystein Amellem :1( 239) Immunosystems, Dynal
Rm 1-6337, Rochester, NY 14642 Biotech ASA, PO Box 114, Smestad, N-0309,
NORWAY
Ruedi Aebersold :4( 437) The Institute for Systems
Biology, 1441 North 34th Street, Seattle, WA 98103- Patrick Amstutz :1( 497) Department of
4098 Biochemistry, University of Zfrich, Winterthurerstr.
190, Zurich, CH-8057, SWITZERLAND
Ueli Aebi :3( 233, 241) ME Muller Institute for
Microscopy, Biozentrum, University of Basel, Jens .S Andersen :4( 427) Protein Interaction
Klingelbergstr. 50/70, Basel, CH-4056, Laboratory, University of Southern Denmark m
SWITZERLAND Odense, Campusvej 55, Odense M, DK-5230,
DENMARK
Cheol-Hee Ahn :4( )92 School of Materials Science
and Engineering, Seoul National University, Seoul, Mads Hald Andersen :1( )79 Tumor Immunology
151-744, SOUTH KOREA Group, Institute of Cancer Biology, Danish Cancer
Society, Strandboulevarden ,94 Copenhagen, DK-
Natalie G. Ahn :4( 443) Department of Chemistry
2100, DENMARK
& Biochemistry, University of Colorado, 512 UCB,
Boulder, CO 80309 Helena Andersson :4( )36 Bioscience at Novum,
Karolinska Institutet, Halsovagen 7-9, Huddinge,
Ramiro Alberio :4( )54 School of Biosciences,
141-ES ,75 SWEDEN
University of Nottingham, Sutton Bonington,
Loughborough, Leics, LE12 5RD, UNITED Peter W. Andrews :1( 183) Department of
KINGDOM Biomedical Science, The University of Sheffield, Rm
2B 238, Sheffield, $10 2TN, UNITED KINGDOM
Donna G. Albertson :3( 445) Cancer Research
Institute, Department of Laboratory Medicine, The Elsa Anes :2( )75 Faculdade de Farmacia,
University of California, San Francisco, Box 0808, San Universidade de Lisboa, .vA Forcas Armadas, Lisboa,
Francisco, CA 94143-0808 1649-019, PORTUGAL
Heiner Albiez :1( 291) Department of Biology II, James M. Angelastro :1( 171) Department of
Ludwig-Maximilians University of Munich, Munich, Pathology and Center for Neurobiology and
GERMANY Behavior, Columbia University College of Physicians
xxiii
xxiv SROTUBIRTNOC
and Surgeons, 036 West 168th Street, New York, NY Werner Baschong :3( )5 EM Muller Institute for
23001 Microscopy, Biozentrum, University of Basel,
Klingelbergstrasse 50/70, Basel, CH-4056,
Sergey .V Anisimov :4( 103) Molecular Cardiology
SWITZERLAND
Unit, National Institute on Aging, NIH, 5600 Nathan
Shock Drive, Baltimore, MD 21224 Philippe I. H. Bastiaens :3( 153) Cell Biology and
Cell Biophysics Program, European Molecular
Celia Antonio :2( 379) Department of Biochemistry
Biology Laboratory, Meyerhofstrasse ,1 Heidelberg,
& Molecular Biophysics, College of Physicians &
,71196 GERMANY
Surgeons, Columbia University, 107 W 168ST HHSC
,427 New York, NY 71196 Jiirgen .C Becker :1( 103) Department of
Dermatology, University of W6rzburg, Sanderring ,2
Shigehisa Aoki :1( 411) Department of Pathology
W6rzburg, 97070, GERMANY
& Biodefence, Faculty of Medicine, Saga University,
Nebeshima 5-1-1, Saga, 849-8501, JAPAN Martin B6h6 :4( 149) Department of Nuclear
Medicine, Philipp's-University of Marburg,
Ron D. Appel :4( 207) Swiss Institute of
Baldingerstrat~e, Marburg/Lahn, D-35043,
Bioinformatics, CMU, Rue Michel Servet ,1 Geneva ,4
GERMANY
CH-1211, SWITZERLAND
Thomas M. Behr :4( 149) Department of Nuclear
Rolf Apweiler :4( 469) EMBL Outstation, European
Medicine, Philipp's-University of Marburg,
Bioinformatics Institute, Wellcome Trust Genome
Baldingerstrat~e, Marburg, D-35043, GERMANY
Campus, Hinxton, Cambridge, CB10 1SD, UNITED
KINGDOM Stefanie Benesch :2( 399) Department of Cell
Biology, Gesellschaft fur Biotechnoogische
Nobukazu Araki :2( 147) Department of Histology
Forschung, Mascheroder Weg ,1 Braunschweig, -D
and Cell Biology, School of Medicine, Kagawa
,42183 GERMANY
University, Mki, Kagawa, 761-0793, JAPAN
Christopher M. Armstrong :4( 295) Dana Faber Aaron Bensimon :3( 429) Laboratoire de
Biophysique de I'ADN, Departement des
Cancer Institute, Harvard University, 44 Binney
Biotechnologies, Institut Pasteur, 52 rue du .rD Roux,
Street, Boston, MA 51120
Paris Cedex ,51 F-75724, FRANCE
Anthony .J Ashford :2( 155) Antibody Facility, Max
Planck Institute of Molecullar Cell Biology and John .J M. Bergeron :2( )14 Department of
Genetics, Pfotenhauerstrsse 108, Dresden, D-01307, Anatomy and Cell Biology, Faculty of Medicine,
GERMANY McGill University, STRATHCONA Anatomy &
Dentistry Building, Montreal, QC, H3A ,2B2
Daniel Axelrod :3( )91 Dept of Physics &
CANADA
Biophysics Research Division, University of
Michigan, Ann Arbor, MI 5501-90184 Michael W. Berns :3( 351) Beckman Laser Institute,
University of California, Irvine, 1002 Health Sciences
Sheree Bailey :1( 475) Dept of Immunology,
Road ,E Irvine, CA 5741-79629
Allergy and Arthritis, Flinders Medical Centre and
Flinders University, Bedford Park, Adelaide, ,AS Joseph .R Bertino :1( 315) The Cancer Institute of
5051, SOUTH AUSTRALIA New Jersey, 591 Little Albany Street, New Brunswick,
NJ 10980
Nathalie Q. Balaban :2( 419) Department of
Physics, The Hebrew University-Givat Ram, Racah Paulo Bianco :1( )97 Dipartimento di Medicina
Institute, Jerusalem, 91904, ISRAEL Sperimentale e Patologia, Universita 'La Sapienza',
Viale Regina Elena 324, Roma, ,16100-1 YLATI
William E. Balch :2( 209) Department of Cell and
Molecular Biology, The Scripps Research Institute, Hans Kaspar Binz :1( 497) Department of
05501 North Torrey Pines Road, La Jolla, CA 92037 Biochemistry, University of Z~irich, Winterthurerstr.
,091 Z6rich, CH-8057, SWITZERLAND
Debabrata Banerjee :1( 315) Department of
Medicine, Cancer Institute of New Jersey, 591 Little .R Curtis Bird :1( 247) Department of Pathobiology,
Albany Street, New Brunswick, NJ 08903 Auburn University, Auburn, AL 94863
Jiri Bartek :4( 253) Department of Cell Cycle and Mina .J Bissell :1( 139) Life Sciences Division,
Cancer, Danish Cancer Society, Strandboulevarden Lawrence Berkeley National Laboratory, 1 Cyclotron
,94 Copenhagen, DK-2100, DENMARK Road, Bldg 83-101, Berkeley, CA 02749
CONTRIBUTORS XXV
Stephanie Blackwood :3( 445) Cancer Research Nicholas H. Brown :3( )77 Wellcome Trust/Cancer
Institute, University of California San Francisco, PO Research UK Institute and Departmentt of Anatomy,
xoB 0808, San Francisco, CA 8080-34149 University of Cambridge, Tennis Court Road,
Cambridge, 2BC 1QR, UNITED KINGDOM
Blagoy Blagoev :4( 427) Protein Interaction
Laboratory, University of Southern Denmark m Heather L. Brownell :2( 329, 341) Office of
Odense, Campusvej ,55 Odense M, DK-5230, Technology Licensing and Industry Sponsored
DENMARK Research, Harvard Medical School, 52 Shattuck
Street, Gordon Hall of Medicine, Room 414, Boston,
Kenneth R. Boheler :4( 103) Laboratory of
MA 51120
Cardiovascular Science, National Institute on Aging,
NIH, 5600 Nathan Shock Drive, Baltimore, MD Damien Brunner :3( )96 Cell Biology and Cell
5286-42212 Biophysics Programme, European Molecular Biology
Laboratory, Meyerhofstrasse ,1 Heidelberg, ,71196-D
Michelle A. Booden :1( 345) Lineberger
GERMANY
Comprehensive Cancer Center, University of North
Carolina at Chapel Hill, Chapel Hill, NC 5927-99572 Suzannah Bumpstead :3( 463) Genotyping / Chr
,02 The Wellcome Trust Sanger Institute, The
Gary G. Borisy :3( 277) Department of Cell and
Wellcome Trust Genome Campus, Hinxton,
Molecular Biology, Northwestern University Medical
Cambridge, CB10 1SA, UNITED KINGDOM
School, Chicago, IL 2703-1106
Deborah C. Burford :3( 403) Wellcome Trust,
Elliot Botvinick :3( 351) Beckman Laser Institute,
Sanger Institute, The Wellcome Trust Genome
University of California, Irvine, 1002 Health Sciences
Campus, Hinxton, Cambridge, CB10 1SA, UNITED
Road, East, Irvine, CA 5741-79629
KINGDOM
G6rard Bouchet :4( 207) Swiss Institute of
Gerald Burgstaller :2( 161) Department of Cell
Bioinformatics (SIB), CMU, rue Michel-Servet ,1
Biology, Institute of Molecular Biology, Austrian
Gen6ve ,4 CH-1211, SWITZERLAND
Academy of Sciences, Billrothstrasse ,11 Salzburg,
Rosemary Boyle :4( 437) The Institute for Systems A-5020, AUSTRIA
Biology, 1441 North 34th St., Seattle, WA 90189
Ian M. Caldicott :1( 157)
Susanne Brandfass :1( 563) Department of
Angelique S. Camp :1( 457) Gene Therapy Centre,
Biochemistry and Cell Biology, Max Planck Institute
University of North Carolina at Chapel Hill, 9117
of Biophysical Chemistry, Am Fat~berg ,11 Gottingen,
Thurston-Bowles (G44 Wilson Hall), Chapel Hill, NC
D-37077, GERMANY
2537-99572
Pascal Braun :4( )37 Department of Chemistry and
Keith H. .S Campbell :4( )54 School of Biosciences,
Chemical Biology, Harvard University, 21 Oxford
Sutton Bonington, Loughborough, Leics, LE12 5RD,
Street, Cambridge, MA 83120
UNITED KINGDOM
Steven A. Braut :4( 121) Department of Anatomy
and Structural Biology, Golding # 601, Albert Einstein u Cao :1( 373) Microbiology & Tumor Biology
College of Medicine of Yeshiva University, 0031 Center, Karolinska Institute, Room: Skrivrum (G415),
Morris Park Avenue, Bronx, NY 16401 xoB 280, Stockholm, SE-171 ,77 SWEDEN
Alvis Brazma :4( )59 EMBL Outstation--Hinxton, Maria Carmo-Fonseca :2( 277, :3 419) Institute of
European Bioinformatics Institute, Wellcome Trust Molecular Medicine, Faculty of Medicine, University
Genome Campus, Hinxton, Cambridge, CB10 1SD, of Lisbon, .vA Prof. Egas Moniz, Lisbon, ,820-9461
UNITED KINGDOM PORTUGAL
.J David Briley :3( 471) Department of Genomic .T Carneiro :3( 419) Faculty of Medicine, Institute of
Sciences, Glaxo Wellcome Research and Molecular Medicine, University of Lisbon, .vA Prof.
Development, 5 Moore Drive, Research Triangle Egas Moniz, Lisboa, 1649-028, PORTUGAL
Park, NC 8933-90772
Nigel .P Carter :2( 133) The Wellcome Trust, Sanger
Simon Broad :1( 133) Keratinocyte Laboratory, Institute, The Wellcome Trust, Genome Campus,
London Research Institute, 44 Lincoln's Inn Fields, Hinxton, Cambridge, CB10 1SA, UNITED
London, WC2A 3PX, UNITED KINGDOM KINGDOM
xxvi SROTUBIRTNOC
C61ia Carvalho :3( 419) Faculty of Medicine, Juno Choe :1( 269) Institute for Systems Biology,
Institute of Molecular Medicine, University of 1441 N. 34th ,tS Seattle, WA 98103
Lisbon, .vA Prof. Egas Moniz, Lisboa, 1649-028,
Claus R. .L Christensen :1( 363) Department of
PORTUGAL
Molecular Cancer Biology, Danish Cancer Society,
Lucy A. Carver :2( )11 Cellular and Molecular Institute of Cancer Biology, Strandboulevarden ,94
Biology Program, Sidney Kimmel Cancer Center, Copenhagen, DK-2100, DENMARK
10835 Altman Row, San Diego, CA 12129
Theodore Ciaraldi :1( 121) Veterans Affairs Medical
Doris Cassio :1( 231, :3 387) INSERM U-442: Center, University of California, San Diego, 9500
Signalisation cellulaire et calcium, Bat 443, Universite Gilman Drive, La Jolla, CA 1119-39029
Paris-Sud, Street George Clemenceau Pack, 444,
Aaron Ciechanover :4( 351) Center for Tumor and
Orsay, Cedex, F-91405, FRANCE
Vascular Biology, The Rappaport Faculty of Medicine
Chris Catton :3( 207) Department of Zoology, and Research Institute, Technion-Israel Institute of
University of Oxford, South Parks Road, Oxford, Technology, POB 9649, Efron Street, Bat Galim, Haifa,
1XO 3PS, UNITED KINGDOM 31096, ISRAEL
Julio .E Celis :1( 527, :4 ,96 165, 219, 243, 289) Mark .S .F Clarke :2( 233, :4 )5 Department of
Danish Cancer Society, Institute of Cancer Biology Health and Human Performance, University of
and Danish Centre for Translational Breast Cancer Houston, 3855 Holman Street, GarrisonmRm 104D,
Research, Strandboulevarden ,94 Copenhagen O, Houston, XT 77204-6015
DK-2100, DENMARK
Martin Clynes :1( 335) National Institute for
Pierre Chambon :3( 501) Institut de G6n6tique Cellular Biotechnology, Dublin City University,
et de Biologie Mol6culaire et Cellulaire, 1 rue Glasnevin, Dublin, ,9 IRELAND
Laurent Fries, B.P.10142, Illkirch CEDEX, F-67404,
Philippe Collas :1( 207) Institute of Medical
FRANCE
Biochemistry, University of Oslo, PO Box 2111
Francis Ka-Ming Chan :2( 355) Department of Blindern, Oslo, 0317, NORWAY
Pathology, University of Massachusetts Medical
Kristen Correia :4( )53 Krumlauf Lab, Stowers
School, Room $2-125, 55 Lake Avenue North,
Institute for Medical Research, 1000 East 50th Street,
Worcester, MA 01655
Kansas City, MO 64110
Ming-Shien Chang :3( )78 Department of Physics,
Pascale Cossart :2( 407) Unite des Interactions
Duke University, 701 Physics Bldg, Durham, NC
Bacteries-Cellules/Unit6 INSERM 604, Institut
27708-1000
Pasteur, ,82 rue du Docteur Roux, Paris Cedex ,51
F-75724, FRANCE
Samit Chatterjee :2( 241) Margaret M. Dyson
Vision Research Institute, Department of Thomas Cremer :1( 291) Department of Biology ,II
Ophthalmology, Weill Medical College of Cornell Ludwig-Maximilians University of Munich, Munich,
University, 1300 York Avenue, New York, NY 12001 80333, GERMANY
Sandeep Chaudhary :1( 121) Veterans Affairs Robert A. Cross :2( 371) Molecular Motors Group,
Medical Center, San Diego (V111G), 3350 La Jolla Marie Curie Research Institute, The Chart, Oxted,
Village Drive, San Diego, CA 16129 Surrey, RH8 0TE, UNITED KINGDOM
Jingwen Chen :3( 471) Department of Genomic Matthew .E Cunningham :1( 171) Hospital for
Sciences, Glaxo Wellcome Research and Special Surgery, New York Hospital, 520 .E 70th
Development, 5 Moore Drive, Research Triangle Street, New York, NY 12001
Park, NC 27709
No61ia Cust6dio :3( 419) Faculty of Medicine,
Yonglong Chen :1( 191) Institute for Biochemistry Institute of Molecular Medicine, University of
and Molecular Cell Biology, University of Lisbon, .vA Prof. Egas Moniz, Lisboa, 1649-028,
Goettingen, Justus-von-Liebig-Weg ,11 G6ttingen, PORTUGAL
D-37077, GERMANY
Zbigniew Darzynkiewwicz :1( 279) The
Yong Woo Cho :4( )92 Akina, Inc., Business & Cancer Research Institute, New York Medical
Technology Center, 1291 Cumberland Ave., #E130, College, 91 Bradhurst Avenue, Hawthorne, NY
West Lafayette, IN 47906 23501
SROTUBIRTNOC xxvii
Ilan Davis :3( 187) Wellcome Trust Centre for Cell James Fred Dice :4( 345) Department Physiology,
Biology, Institute of Cell and Molecular Biology, The Tufts University School of Medicine, 631 Harrison
University of Edinburgh, Michael Swann Building, Ave, Boston, MA 11120
The King's Buildings, Mayfield Road, Edinburgh,
Thomas J. Diefenbach :4( 307) Department of
EH9 3JR, SCOTLAND
Physiology, Tufts University School of Medicine, 631
Stephen C. De Rosa :1( 257) Vaccine Research Harrison Avenue, Boston, MA 11120
Center, National Institutes of Health, 40 Convent Dr.,
Chris Dinant :2( 121) Biomolecular Sciences,
Room 5610, Bethesda, MD 20892-3015
UMIST, PO Box ,88 Manchester, M60 1QD, UNITED
KINGDOM
Nicholas M. Dean :3( 523) Functional Genomics,
GeneTrove, GeneTrove (a division of Isis Isis Da-Qiao Ding :3( 171) Structural Biology Section
Pharmaceuticals, Inc.), 2292 Faraday Avenue, and CREST Research Project, Kansai Advanced
Carlsbad, CA 92008 Research Center, Communications Research
Laboratory, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku,
Anne Dell :4( 415) Department of Biological
Kobe, 651-2492, JAPAN
Sciences, Biochemistry Building, Imperial College
of Science, Technology & Medicine, Biochemistry Gilles Divita :4( )31 Centre de Recherche en
Building, London, SW7 ,YA2 UNITED KINGDOM Biochimie Macromol6culaire (UPR 1086), Centre
National de la Recherche Scientifique (CNRS), 1919
Panos Deloukas :3( 463) The Wellcome Trust,
Route de Mende, Montpellier Cedex ,5 F-34293,
Sanger Institute, Hinxton, Cambridge, CB10 1SA,
FRANCE
UNITED KINGDOM
Eric .P Dixon :1( 483) TriPath Oncology, 4025
Nicolas Demaurex :3( 163) Department of Cell
Stirrup Creek Drive, Suite 400, Durham, NC 27703
Physiology and Metabolism, University of Geneva
Medical Center, 1 Michel-Servet, Geneva, CH-1211, Bernhard Dobberstein :2( 215) Zentrum fur
SWITZERLAND Molekulare Biologie, Universitat Heidelberg, Im
Neuenheimer Feld 282, Heidelberg, D-69120,
Chris Denning :4( )54 Division of Animal GERMANY
Physiology, School of Biosciences, Institute of
Lynda J. Donald :4( 457) Department of Chemistry,
Genetics Room C15, University of Nottingham,
University of Manitoba, Room 135 Parker Building,
Queens Medical Centre, Nottingham, NG7 2UH,
Winnipeg, MB, R3T 2N2, CANADA
UNITED KINGDOM
Wolfgang R. G. Dostmann :2( 299) Department of
Ami Deora :2( 241) Margaret M. Dyson Vision
Pharmacology, University of Vermont, Health
Research Institute, Department of Ophthalmology,
Science Research Facility 330, Burlington, VT
Weill Medical College of Cornell University, 1300
05405-0068
York Avenue, New York, NY 12001
Adam Douglass :3( 129) Department of Cellular
Julien Depollier :4( )31 Centre de Recherche en
and Molecular Pharmacology, The University of
Biochimie Macromol6culaire (UPR 1086), Centre
California, San Francisco, School of Medicine,
National de la Recherche Scientifique (CNRS), 1919
Medical Sciences Building, Room $1210, 315
Route de Mende, Montpellier Cedex ,5 F-34293,
Parnassus Avenue, San Francisco, CA 94143-0450
FRANCE
Kate Downes :3( 463) Genotyping / Chr 20, The
Channing J. Der :1( 345) Department of
Wellcome Trust, Sanger Institute, The Wellcome Trust
Pharmacology, University of North Carolina at
Genome Campus, Hinxton, Cambridge, CB10 1SA,
Chapel Hill, Lineberger Comprehensive Cancer
UNITED KINGDOM
Center, Chapel Hill, NC 27599
Harry W. Duckworth :4( 457) Department of
Bart Devreese :4( 259) Department of Biochemistry, Chemistry, University of Manitoba, Room 135
Physiology and Microbiology, University of Ghent, Parker Building, Winnipeg, MB, R3T 2N2,
K.L. Ledeganckstraat ,53 Ghent, B-9000, BELGIUM CANADA
Alberto Diaspro :3( 201) Department of Physics, Derek M. Dykxhoorn :3( 511) CBR Institute for
University of Genoa, Via Dodecaneso 33, Genoa, Biomedical Research, Harvard Medical School, 200
,64161-1 ITALY Longwood Ave, Boston, MA 02115
xxviii SROTUBIRTNOC
Lars Dyrskjot :4( )38 Clinical Biochemical Patricio Fern~indez-Silva :2( )96 Dept of
Department, Molecular Diagnostic Laboratory, Biochemistry and Molecular and Cellular Biology,
Aarhus University Hospital, Skejby, Univeridad de Zaragoza, Miguel Servet ,771
Brendstrupgaardvej, Aarhus N, DK-8200, Zaragoza, E-50013, SPAIN
DENMARK
Erika Fern~indez-Vizarra "2( )96 Dept of
Christoph Eckerskorn :4( 157) Protein Analytics, Biochemistry and Molecular and Cellulary Biology,
Max Planck Institute for Biochemistry, Klopferspitz Universidad de Zaragoza, Miguel Servet, ,771
,81 Martinsried, D-82152, GERMANY Zaragoza, E-50013, SPAIN
Glenn S. Edwards :3( )78 Department of Physics, Patrick .F Finn :4( 345) Department of Physiology,
Duke University, 122 LEF Bldg, xoB 90305, Durham, Tufts University School of Medicine, 631 Harrison
NC 5030-80772 Ave, Boston, MA 11120
Andreas .A O. Eggert :1( 103) Department of Kevin L. Firth :2( 329, :2 341) ASK Science Products
Dermatology, Julius-Maximillians University, Inc., 784 Victoria ,tS Kingston, Ontario, L7K 3Z8,
Josef-Schneider-Str. ,2 W6rzburg, 97080, CANADA
GERMANY
Raluca Fliikiger-Gagescu :2( )72 Unitec m
Maria Ekstri~m :4( )36 Bioscience at Novum, Office of Technology Transfer, University of Geneva
Karolinska Institutet, Huddinge, SE-141 ,75 and University of Geneva Hospitals, ,42 Rue
SWEDEN G6n6ral-Dufour, Geneva ,4 CH-1211,
SWITZERLAND
Andreas Engel :3( 317) Maurice .E re116M Institute
for Microscopy at the Biozentrum, University of Leonard .J Foster :4( 363, 427) Protein Interaction
Basel, Klingelbergstrasse ,07 Basel, CH-4056, Laboratory, University of Southern Denmark,
SWITZERLAND Odense, Campusvej ,55 Odense M, DK-5230,
DENMARK
Anne-Marie Engel :1( 353) Bartholin Institutte,
Bartholinsgade ,2 Copenhagen ,K DK-1356,
Dimitrios Fotiadis :3( 317) M.E. Miiller Institute
DENMARK
for Microscopy at the Biozentrum, University of
Jos6 A. Enriquez :2( )96 Department of Basel, Klingelbergstrasse ,07 Basel, CH-4056,
Biochemistry and Molecular and Cellular Biology, SWITZERLAND
Universidad de Zaragoza, Miguel Servet, ,771
Patrick .L .T M. Frederix "3( 317) M.E. re116M
Zaragoza, E-50013, SPAIN
Institute for Microscopy at the Biozentrum,
Rachel Errington :1( 305) Department of Medical University of Basel, Klingelbergstrasse ,07 Basel,
Biochemistry and Immunology, University of Wales CH-4056, SWITZERLAND
College of Medicine, Heath Park, Cardiff, CF14 4XN,
Marcus Frohme :4( 113) Functional Genome
UNITED KINGDOM
Analysis, German Cancer Research Center, Deutsches
Virginia Espina :3( 339) Microdissection Core Krebsforschungszentrum, Im Neuenheimer Feld 580,
Facility, Laboratory of Pathology, National Cancer Heidelberg, D-69120, GERMANY
Institute, 9000 Rockville Pike, Building ,01 Room
Masanori Fujimoto :4( 197) Department of
BIB53, Bethesda, MD 20892
Biochemistry and Biomolecular Recognition,
H. Dariush Fahimi :2( )36 Department of Anatomy Yamaguchi University School of Medicine, ,1-1-1
and Cell Biology ,II University of Heidelberg, Im Minami-kogushi, Ube, Yamaguchi, ,5058-557
Neuenheimer Feld 307, Heidelberg, D-69120, JAPAN
GERMANY
Margarida Gama-Carvalho :2( 277) Faculty of
Federico Federici :3( 201) Department of Physics, Medicine, Institute of Molecular Medicine, University
University of Genoa, aiV Dodecaneso ,33 Genoa, of Lisbon, .VA Prof. Egas Moniz, Lisbon, ,820-9461
,64161-1 YLATI PORTUGAL
Daniel L. Feeback :2( 233, :4 )5 Space and Life Henrik Garoff :1( 419, :4 )36 Unit for Cell Biology,
Sciences Directorate, NASA-Johnson Space Center, Center for Biotechnology. Karolinska Institute,
3600 Bay Area Blvd, Houston, XT 85077 Huddinge, SE-141 ,75 SWEDEN
CONTRIBUTORS xxix
Susan M. Gasser :2( 359) Friedrich Miescher Frank L. Graham :1( 435) Department of Biology,
Institute fr Biomedical Research, Maulbeerstrasse ,66 McMaster University, Life Sciences Building, Room
Basel, CH-1211, SWITZERLAND 430, Hamilton, Ontario, L8S 4K1, CANADA
Kristine G. Gaustad :1( 207) Institute of Medical Claude Granier :1( 519) UMR 5160, Facult6 de
Biochemistry, University of Oslo, PO Box 2111 Pharmacie, 51 .vA Charles Flahault, Montpellier
Blindern, Oslo, 0317, NORWAY Cedex ,5 BP 14491, 34093, FRANCE
Benjamin Geiger :2( 419) Dept. of Molecular Cell Lloyd A. Greene :1( 171) Department of Pathology
Biology, Weizman Institute of Science, Wolfson and Center for Neurobiology and Behavior,
Building, Rm 617, Rehovot, 76100, ISRAEL Columbia University, College of Physicians and
Surgeons, 036 .W 168th Street, New York, NY
Kris Gevaert :4( 379, :4 457) Dept. Medical Protein
23001
Research, Flanders Interuniversity Institute for
Biotechnology, Faculty of Medicine and Health Susan M. Gribble :3( 403) Sanger Institute, The
Sciences, Ghent University, Instituut Rommelaere m Wellcome Trust, The Wellcome Trust Genome
Blok D, Albert Baertsoenkaai ,3 Gent, B-9000, Campus, Hinxton, Cambridge, CB10 1SA, UNITED
BELGIUM KINGDOM
Jilur Ghori :3( 463) Genotyping / Chr 20, The Gareth Griffiths :2( 57, :3 299) Department of Cell
Wellcome Trust, Sanger Institute, The Wellcome Biology, EMBL, Postfach 102209, Heidelberg, D-
Trust, Genome Campus, Hinxton, Cambridge, CB10 69117, GERMANY
1SA, UNITED KINGDOM
Sergio Grinstein :3( 163) Cell Biology Program,
Alasdair J. Gibb :1( 395) Department of Hospital for Sick Children, 555 University Avenue,
Pharmacology, University College London, Gower Toronto, Ontario, M5G 1X8, CANADA
Street, London, WCIE GBT, UNITED KINGDOM
Pavel Gromov :1( 527, :4 ,96 165, 243, 289) Institute
Mario Gimona :1( 557, :2 161, :4 145) Department of Cancer Biology and Danish Centre for
of Cell Biology, Institute of Molecular Biology, Translational Breast Cancer Research, Danish Cancer
Austrian Academy of Sciences, Billrothstrasse ,11 Society, Strandboulevarden 49, Copenhagen, DK-
Salzburg, A-5020, AUSTRIA 2100, DENMARK
David A. Glesne :1( 165) Biosciences Division, Irina Grornova :4( 219) Department of Medical
Argonne National Laboratory, 9700 South Cass Biochemistry and Danish Centre for Translational
Avenue, Argonne, IL 60439-4844 Breast Cancer Research, Danish Cancer Society,
Strandboulevarden 49, Copenhagen, DK-2100,
Martin Goldberg :3( 325) Science Laboratories,
DENMARK
University of Durham, South Road, Durham, DH1
3LE, UNITED KINGDOM Dale E Gruber :1( )33 Cell Culture Research and
Development, GIBCO/Invitrogen Corporation, 3175
Kenneth N. Goldie :3( 267) Structural and
Staley Road, Grand Island, NY 14072
Computational Biology Programme, EMBL,
Meyerhofstrasse ,1 Heidelberg, D-69117, Markus Grubinger :4( 145) Institute of Physics and
GERMANY Biophysics, University of Salzburg, Hellbrunnerstr.
34, Salzburg, A-5020, AUSTRIA
Jon W. Gordon :3( 487) Geriatrics and Adult
Development, Mount Sinai School of Medicine, Jean Gruenberg :2( 27, 201) Department of
One Gustave .L Levy Place, New York, NY Biochemistry, University of Geneva, 30, quai
92001 Ernest Ansermet, Geneva ,4 CH-1211,
SWITZERLAND
Angelika Gi~rg :4( 175) Fachgebiet Proteomik,
Technische Universit~it M~inchen, Am Forum ,2 Stephanie .L Gupton :3( 137) 10550 North Torrey
Freising Weihenstephan, D-85350, GERMANY Pines Road, CB 163, La Jolla, CA 92037
Martin Gotthardt :4( 149) Department of Nuclear Cernal Gurkan :2( 209) Department of Cell and
Medicine, Philipp's-University of Marburg, Molecular Biology, The Scripps Research Institute,
Baldingerstrat~e, Marburg/Lahn, D-35043, 10550 North Torrey Pines Road, La Jolla, CA
GERMANY 92037
XXX CONTRIBUTORS
Martin Guttenberger :4( 131) Zentrum ri~f Frederic Heitz :4( )31 Centre de Recherche en
Molekulariologie der Pflanzen, Universitat T/~bingen, Biochimie Macromol6culaire (UPR 1086), Centre
Entwicklungsgenetik, Auf der Morgenstelle ,3 National de la Recherche Scientifique (CNRS), 1919
Tiibingen, D-72076, GERMANY Route de Mende, Montpellier Cedex ,5 F-34293,
FRANCE
Thomas Haaf :3( 409) Institute for Human
Genetics, Johannes Gutenberg-Universit~it Mainz, Johannes W. Hell :2( )58 Department of
55101, Mainz, D-55131, GERMANY Pharmacology, University of Iowa, 2152 Bowen
Science Building, Iowa City, IA 52242
Christine M. Hager-Braun :1( 511) Health and
Human Services, NIH National Institute of Kai Hell :4( 269) Adolf-Butenandt-Institut fur
Environmental Health Sciences, MD F0-04, PO xoB Physiologische Chemie, Lehrstuhl: Physiologische
12233, Research Triangle Park, NC 27709 Chemie, Universitat Munchen, Butenandtstr. 5,
Geb~iude ,B Munchen, D-81377, GERMANY
Anne-Marl H~kelien :1( 207) Institute of Medical
Biochemistry, Institute of Medical Biochemistry, Robert R. Henry :1( 121) Veterans Affairs Medical
University of Oslo, PO Box 1112 Blindern, Oslo, 0317, Center, San Diego (V111G), 3350 La Jolla Village
NORWAY Drive, San Diego, CA 92161
Fiona C. Flalliday :1( 395) GlaxoSmithKline, Johan Hiding :2( )54 G6teborg University, Institute
Greenford, Middlesex, UB60HE, UNITED of Medical Biochemistry, PO Box 440, G6teborg, -ES
KINGDOM 403-50, SWEDEN
Gerald Hammond :2( 223) Molecular Yasushi Hiraoka :3( 171) Structural Biology Section
Neuropathobiology Laboratory, Cancer Reasearch and CREST Research Project, Kansai Advanced
UK London Research Institute, 44 Lincoln's Inn Research Center, Communications Research
Fields, London, WC2A 3PX, UNITED KINGDOM Laboratory, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku,
Kobe, 651-2492, JAPAN
Klaus Hansen :4( 253)
Mary M. Hitt :1( 435) Department of Pathology &
Hironobu I-Iarada :1( 367) Department of Molecular Medicine, McMaster University, 1200
Neurosurgery, Ehime University School of Medicine, Main Street West, Hamilton, Ontario, L8N 3Z5,
Shitsukawa, Toon-shi, Ehime, 791-0295, JAPAN CANADA
Robert J. Hay :1( 43, 49, 573) Viitro Enterprises Julie Hodgkinson :3( 307) School of
Incorporated, 1113 Marsh Road, PO xoB 328, Crystallography, Birkbeck College, Unversity of
Bealeton, AV 22712 London, Malet Street, London, WCIE 7HX, UNITED
KINGDOM
Izumi Hayashi :1( 151) National Medical Center
and Beckman Research Institute, Division of Klaus .P Hoeflich :2( 307) Division of Molecular
Neurosciences, City of Hope, 1500 .E Duarte Rd, and Structural Biology, Ontario Cancer Institute,
Duarte, CA 91010-3000 Department of Medical Biophysics, University of
Toronto, 016 University Avenue, 7-707A, Toronto,
Timothy A. Haystead :4( 265) Department of
Ontario, M5G 2M9, CANADA
Pharmacology and Cancer Biology, Duke University
Medical Center, xoB 3813 Med Ctr, Durham, NC Tracy .L Hoffman :1( )12 ATCC, P.O. xoB 1549,
27710 Manassas, AV 20108
Rebecca I-Ieald :2( 379) Molecular and Cell Biology gr~iJ D. Hoheisel :4( 113) Functional Genome
Department, University of California, Berkeley, Analysis, German Cancer Research Center, Deutsches
Berkeley, CA 94720-3200 Krebsforschungszentrum, Im Neuenheimer Feld 580,
Heidelberg, D-69120, GERMANY
Florence Hediger :2( 359) Department of Molecular
Biology, University of Geneva, ,03 Quai Ernest Thomas Hollemann :1( 191) Institute for
Ansermet, Geneva, CH-1211, SWITZERLAND Biochemistry and Molecular Cell Biology, University
of Goettingen, Justus-von-Liebig-Weg ,11 G6ttingen,
Rainer Heintzmann :3( )92 Randall Division of Cell
D-37077, GERMANY
and Molecular Biophysics, King's College London,
Guy's Campus, London, 1ES 1UL, UNITED Caterina I-Iolz :4( )75 PSF biotech AG, Huebnerweg
KINGDOM ,6 Berlin, D-14059, GERMANY