Table Of ContentS. Furusaki . I. Endo . R. Matsuno (Eds.)
Biochemical _ __
_ Engineering _ _
_ for 2001 _ __
Proceedings of Asia-Pacific
Biochemical Engineering Conference 1992
With 623 Illustrations
Springer-Verlag
Tokyo Berlin Heidelberg
New York London Paris
Hong Kong Barcelona
Professor Shintaro Furusaki
Department of Chemical Engineering, Faculty of Engineering, The University
of Tokyo, 3-1 Hongo 7-chome, Bunkyo-ku, Tokyo 113, Japan
Dr. Isao Endo
Head of Chemical Engineering Laboratory, The Institute of Physical and
Chemical Research, 2-1 Hirosawa, Wako-shi, Saitama 351-01, Japan
Professor Ryuichi Matsuno
Department of Food Science and Technology, Faculty of Agriculture, Kyoto
University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-01, Japan
ISBN -13 :978-4-431-68182-3 e-ISBN -13 :978-4-431-68180-9
DOl: 10.1007/978-4-431-68180-9
© Springer-Verlag Tokyo 1992
Softcover reprint of the hardcover 1st edition 1992
This work is subject to,copyright. All rights are reserved, whether the whole or part of
the material is concerned, specifically the rights of translation, reprinting, reuse of
illustrations, recitation, broadcasting, reproduction on microfilms or in other ways,
and storage in data banks.
The use of registered names, trademarks, etc. in this publication does not imply, even
in the absence of a specific statement, that such names are exempt from the relevant
protective laws and regulations and therefore free for general use.
Preface
The remarkable progress in biotechnology since the 1970s suggested the
possibility of innovation in mass conversion processes using biological
functions. However, it also indicated the importance of viewing a bio
production process as a whole and of improving the efficiencies of up
and down-stream processings harmonically, for which chemical engineers
are responsible. During the past decade, many departments of bio
chemical engineering in universities have been organized and the
direction of research and development in industry has been gradually
settled. Accordingly, the supporting academic societies have focused a
considerable part of their activities on biochemical engineering. In this
context, we may regard the present situation as one where the initial
boost has finished and flowers are blooming. Now is the turning point
as to whether we can be fruitful and whether we can mature in the twenty
first century. The burden on biochemical engineering is very heavy.
This book is the proceedings of the Asia-Pacific Biochemical Engi
neering Conference 1992 (APBioChEC'92), held in Yokohama, Japan
from April 12-15, 1992. The conference was jointly organized by The
Society of Chemical Engineers, Japan, The Korean Institute of Chemical
Engineers, and The American Institute of Chemical Engineers, and is
the second conference, following the first held at Kyungju, Korea in
1990. We hope the proceedings will provide an overview of world trends
in research into biochemical engineering, specific research interests in
the Asia-Pacific region, and various budding research projects which, in
the future, will develop and mature.
In organizing APBioChEC'92, we are indebted to the members of the
organizing committee, especially Drs. K. Arai, T. Kajiuchi, H. Kataoka,
T. Kimura, T. Kobayashi, T. Kodama, K. Matsumoto, M. Matsumura,
and K. Saito. International advisory board members helped us by inviting
many overseas participants to attend APBioChEC'92. The following
session chairpersons assisted us in organizing the conference and review
ing the manuscripts: Drs. K. Funatsu, S. Iijima, H. Ishikawa, S. Katoh,
o. Miyawaki, S. Nagai, K. Nakamura, K. Nakanishi, H. Ohtake,
S. Shioya, S. Tone, T. Yamane, and T. Yoshida.
We are grateful to-the following cosponsors: The Japan Bioindustry
Association; Japan Society of Bioscience, Biotechnology, and Agro
chemistry; The Society of Fermentation Technology, Japan; Japanese
Society of Enzyme Engineering; The Membrane Society of Japan; The
v
VI
Japanese Society of Artificial Organs; Japanese Association for Plant
Tissue Culture; Japanese Association for Animal Cell Technology, and
The Engineering Academy of Japan. Thanks are also due to many
companies listed below and organizations, such as The Asahi Glass
Foundation, Ciba-Geigy Foundation for Promotion of Science, The
Iwatani Naoji Foundation, and The Naito Foundation, for financial
support to the conference.
April, 1992 Shintaro Furusaki
Isao Endo
Ryuichi Matsuno
List of Sponsors
Ajinomoto Co., Ltd.
Asahi Glass Foundation
Cbiyoda Corporation
Ciba-Geigy Foundation
City of Yokohama
Daicel Chemical Industries, Ltd.
Daiichi Pharmaceutical Co., Ltd.
Engineering Academy of Japan
Iwatani Naoji Foundation
Japan Chemical Engineering and
Machinery Co., Ltd.
Japan Gore-Tex Inc.
JGC Corporation
K F Engineering Co., Ltd.
Kanegafuchi Chemical Industry
Co., Ltd.
Kao Corporation
Kawasaki Steel Corporation
Kikkoman Corporation
Kirin Brewery Co., Ltd.
Kureha Chemical Industry Co.,
Ltd.
Kyowa Hakko Kogyo Co., Ltd.
Mitsubishi Kasei Corporation
Mitsubishi Petrochemical Co., Ltd.
Mitsubishi Rayon Co., Ltd.
Mitsui Toatsu Chemicals, Inc.
NGK Insulators, Ltd.
Naito Foundation
Nippon Kayaku Co., Ltd.
Nippon Mining Co., Ltd.
Nippon Synthetic Chemical Indus-
try Co., Ltd.
Nitto Chemical Industry Co., Ltd.
Novonordisk Bioindustry Ltd.
Pfizer Pharmaceuticals Inc.
Shinko Pantec Co., Ltd.
VII
VIII
Shionogi & Co., Ltd.
Shiseido Co., Ltd.
Showa Denko KK
Snow Brand Milk Products Co.,
Ltd.
Sumitomo Chemical Industries,
Co., Ltd.
Sumitomo Heavy Industries, Ltd.
Takeda Chemical Industries, Ltd.
Tanabe Seiyaku Co., Ltd.
Teijin Ltd.
Toagosei Chemical Industry Co.,
Ltd.
Tokuju Kousakusho Co., Ltd.
Tokyo GasCo., Ltd.
Toray Industries, Inc.
Toto Ltd.
Toyo Engineering Corporation
Toyo Jozo Co., Ltd.
Unitika Ltd.
Contents
Preface .................................................................................. V
Chapter 1. Overview
Biochemical Engineering -- Past, Present and Future
A.E. Humphrey................................................................. 3
Strategic Planning and New Directions in Biochemical Engineering
P.L. Rogers ... ... ....... ..... ....... ..... ... ....... ............ ... ......... ...... 8
New Biocatalytic Functions of Microorganisms and Their
Industrial Applications
H. Yamada ......... ... ..... ......... ....... ... ..... .......... ..... ... .... ..... ... 14
Chapter 2. Gene and Protein Engineering
Development of a Bacillus brevis Host-Vector System for Efficient
Heterologous Protein Production and Its Application to Protein
Engineering
H. Yamagata ..... ...... .... ....... ..... ........ ... .... ..... ................... ... 21
Chimeric Receptor Made from IgM and EGFR that Induces
Phosphorylation Upon Binding with Antigen
H. Ueda, M. Kikuchi, S. Yagi, H. Nishimura ......... ... ..... ........ 27
Molecular Cloning of Feline Interferon cDNA by Direct Expression
and Its Mass Production Using Silkworm
N. Nakamura, T. Sudo, Y. Ueda, T. Sakurai, S. Matsuda,
A. Yanai .......................................................................... 31
Expression of Human Calcitonin in Escherichia coli
T. Kaminuma, T. Iida, M. Tajima, M. Yanagi,
S. Fukushima . ....... ..... ....... ................. ... .......... ..... .... ..... .... 35
Control of Gene Expression and Overproduction of Useful
Heterologous Protein by Using an On-Line Sensor
S. Iijima, Y.S. Park, T. Kobayashi ......... ..... ...... ... ....... ..... ..... 39
Expression and Secretion of Cloned Proteins in Recombinant
Saccharomyces cerevisiae
J.H. Seo, H.G. Lim, M., Marten, A. Patkar, M. Peterson ......... 43
Plasmid Loss Probability in Escherichia coli as a Function
of the Growth Rate of Plasmid-Bearing Cells. I: Variation During
Continuous Cultures
N. Nancib, R. Mosrati, J. Boudrant ....................................... 47
IX
x
Plasmid Loss Probability in Escherichia coli as a Function
of the Growth Rate of Plasmid-Bearing Cells. II: Modelling
for Continuous Cultures
R. Mosrati, N. Nancib, J. Boudrant ....................................... 50
Cloning and Expression of the Xylose (Glucose) Isomerase Gene
from the Thermophile Thermus thermophilus HB8
K. Dekker, A. Sugiura, H. Yamagata, K. Sakaguchi,
S. Udaka .......................................................................... 53
Mutagenesis Studies on the Amino Acid Residues Involved in an Iron
Binding Domain and a Catalytic Site of Human 5-Lipoxygenase
S. Ishii, M. Noguchi, T. Matsumoto, M. Noma ....................... 56
Technology of Production of Recombinant Prourokinase
D.V. Sakharov, A.J. Shevelev, L.M. Metlitskaya, M.V. Lapina,
S.P. Domogatsky ............................................................... 59
High Level Expression and Secretion of Biologically Active Leech
Hirudin Variant 1 (HVl) by Escherichia coli
S. Misawa, H. Furuya, H. Matsuda, S. Abe, H. Hayashi ........... 62
Transfer of Isolated Nuclei of Saccharomyces cerevisiae Into
Anucleated Protoplasts of Nectaromyces species
W.T. Lin, R.H. Yano, L.J. Chen .......................................... 65
Chapter 3. Enzyme Engineering
In Vitro Protein Refolding
D.I.C. Wang, J.L. Cleland .......... ........................ ................ 71
Nitrile Hydratase: Investigation of Its Light-Induced Activation
J. Honda, T. Nagamune, Y. Teratani, A. Hirata, H. Sasabe
I. Endo ............................................................................ 76
Cloning of Bacillus licheniformis Amylase Gene and Its Application
to Synthesis of Branched Oligosaccharides
K.H. Park, I.C. Kim, J.R. Kim, B.c. Seo, Y.D. Choi,
D.S. Lee .......................................................................... 80
Aldolases for Use in Carbohydrate Synthesis: Enzymatic Reaction
Engineering as a Tool for Process Optimization
U. Kragl, P. Appel, D. Gygax, o. Ghisalba,
C. Wandrey........................... .................. ......................... 84
Bioconversion of Gaseous Substrate by Methylotrophic Hansenula
polymorpha in a Gas-Solid Bioreactor
C.H. Kim, S.K. Rhee ......................................................... 88
Asymmetric Hydrolysis of Phenylglycidate Ester by Esterase
from Serratia marcescens
T. Shibatani, H. Matsumae, T. Tosa ... .................. ................. 92
D-Threonine Aldolase and Its Application to D-p-Hydroxy-a-Amino
Acid Synthesis
M. Ikemi, T. Morikawa, T. Miyoshi, S. Shimizu, M. Kataoka,
H. Yamada ....................................................................... 96
Improvement of Enzymatic Properties by Hydrophobic Interaction
S. Cao, L. Ma, Y. Feng, S. Guo, H. Yang, Z. Ding ................. 100
XI
The Effect of Amphopathics on the Properties of Enzymes
(Lipase, Cholinesterase, Cellulase)
S. Cao, Y. Feng, T. Yu, H. Yang, S. Liu ............................... 103
Modified Cellulase with Amphiphilic Copolymers: Solubilization
in Organic Solvent
T. Kajiuchi, J.W. Park ........................................................ 106
Aminoacylase-Catalyzed Synthesis of N-Acyl Amino Acid
from Fatty Acid or Its Ethyl Ester and Amino Acid
Y. Kimura, Y. Kobayashi, S. Adachi, R. Matsuno ................... 109
Peptide Synthesis in Organic Solvent with an Immobilized Enzyme
T. Nagayasu, K. Nakanishi .................................................. 112
A Novel Interesterification Process for Fats and Oils Using
Acetone-Dried Fungus Immobilized in Biomass Support Particles
T. Nakashima, S. Kyotani, E. Izumoto, H. Fukuda ... ............... 115
Fractionation of Cellulase Components by Affinity Precipitation:
Production of Cellobiose by Enzymatic Hydrolysis
T. Homma, M. Fujii, T. Kawakami, M. Taniguchi ................... 118
Separation of Positional Isomers by Combined Use of Enzymatic
Hydrolysis and Extraction
H. Ishikawa, K. Ishimi, K. Okajima ...................................... 121
Studies on the Synthesis and Properties of the Mimics
with Glutathione Peroxidase Activity and Preparation
of the Conjugate of Mimic-Antibody
G.M. Luo, W.e. Ma, Z.L. Huang, T.S. Yang ......................... 124
Direct Synthesis of Cyclodextrin in a Heterogeneous Enzyme
Reaction System Containing Insoluble Extruded Starch
Y.H. Lee, D.C. Park .......................................................... 127
Chapter 4. Fermentation Technology
Engineering and Genetic Approaches to Optimization
of Recombinant Fermentation Process
D.D.Y. Ryu, J.Y. Kim ....................................................... 133
Computer-Controlled Fed-Batch Cultivation of a Recombinant
Escherichia coli for Overproduction of Human Interleukin-2
Y.B. Hwang, D.J. Seo, B.H. Chung, Y.H. Park ...................... 138
Production of Recombinant HTLV-I Envelope Protein
M.L. Hong, M.G.S. Yap, B.T. Koh, L. Chan ......................... 142
Computer-Controlled High Cell Density Cultivation of Recombinant
Escherichia coli Producing B-Galactosidase
D.J. Korz, K. Hellmuth, E.A. Sanders,
W-D. Deckwer .................................................................. 146
Use of an Adaptive Control Strategy for the Production of Exotoxin
A from High Density Culture of Recombinant Escherichia coli
J. Hasio, M. Ahluwalia, J.B. Kaufman, T.R. Clem,
J. Shiloach ........................................................................ 150
