Table Of ContentADVANCES IN
BLADDER RESEARCH
ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY
Editorial Board:
NATHAN BACK, State University of New York at Buffalo
IRUN R. COHEN, The Weizmann Institute of Science
DAVID KRTTCHEVSKY, Wistar Institute
ABEL LAJTHA, N. S. Kline Institute for Psychiatric Research
RODOLFO PAOLETTI, University of Milan
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ADVANCES IN
BLADDER RESEARCH
Edited by
Laurence S. Baskin
and
Simon W. Hayward
Department of Urology
University of California, San Francisco
Springer Science+Business Media, LLC
Proceedings of the International Bladder Research Congress, held April 23-25, 1998, in San Francisco, California
ISBN 978-1-4613-7147-2 ISBN 978-1-4615-4737-2 (eBook)
DOI 10.1007/978-1-4615-4737-2
©1999 Springer Science+Business Media New York
Originally published by Kluwer Academic /Plenum Publishers in 1999
Softcover reprint of the hardcover 1st edition 1999
All rights reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means,
electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the
Publisher
This book is dedicated to my parents, Fred and Cynthia Baskin
PREFACE
The aim of Bladder Research Congress, San Francisco, California, April 23-25,
1998, was to provide a forum for authoritative investigators who are actively involved in
the various disciplines which define the leading edges of bladder research. It is important
for such investigators to continue to meet regularly for the purpose of discussing the latest
developments in their individual fields, to analyze the significance of current research, to
discuss new tactics for unresolved problems, to critically evaluate current theories, and to
develop new theories and approaches as needed.
The two and a half day meeting was organized into five half day sessions, with each
session encompassing one of five topics: (1) Epithelial-Mesenchymal Interactions; (2) Ex
tracellular Matrix and Muscle; (3) Nerves and Pharmacology; (4) Infection and Immunol
ogy; and (5) Oncology. Each session was introduced by a moderator followed by five to
six invited expert speakers with time for extensive interaction from the participants. Two
late-afternoon poster sessions allowed further interactions between investigators.
This book documents the proceedings of the Bladder Research Congress. It is organ
ized into the five half-day sessions of the meeting with moderators overview and an edited
transcription of discussions that followed each presentation.
I would like to thank Sarah Burke and the Office of Continuing Medical Education,
USCF; Joanne Hayward, Editorial Assistant; and Miriam Escamilla, Administrative Assis
tant.
I hope you find this resource useful.
Laurence S. Baskin, M.D.
Program Chair
Chief, Pediatric Urology
UCSF
ix
ACKNOWLEDGMENTS
Support from the following organisations is gratefully acknowledged; without this
funding, the meeting would not have taken place.
The National Institutes of Health
UCSF Department of Urology
* * *
Alza Pharmecuticals
American Foundation of Urologic Disease
Astra Tech, Inc.
Bayer Corporation
C.R. Bard, Inc.
CirconACMI
Interstitial Cystitis Association
Laborie Medical Technologies
Matritech
Mentor Urology
National Kidney Foundation of Northern California
Phone-Poulenc Rorer
SmithKline Beecham
xi
CONTENTS
Section I. Epithelial-Mesenchymal Interactions in the Bladder
Overview of Epithelial-Mesenchymal Interactions in the Bladder 3
Gerald R. Cunha
1. Uroplakins as Markers ofUrothelial Differentiation 7
Tung-Tien Sun, Feng-Xia Liang, and Xue-Ru Wu
2. Urothelial Tissue Regulation: Unraveling the Role of the Stroma ............ 19
Jennifer Southgate, Patricia Hamden, Peter J. Selby, David F. M. Thomas,
and Ludwik K. Trejdosiewicz
3. Creation of Bladder Tissue in Vitro and in Vivo: A System for Organ
Replacement ................................................. 31
Anthony Atala
4. Reconstruction of the Urinary Bladder by Auto-Augmentation,
Enterocystoplasty, and Composite Enterocystoplasty ................. 43
Mary T. Comer, David F. M. Thomas, Ludwik K. Trejdosiewicz, and
Jennifer Southgate
5. Epithelial-Mesenchymal Interactions in the Bladder: Implications for Bladder
Augmentation ................................................ 49
Laurence S. Baskin, Simon W. Hayward, Michael S. DiSandro, Ying Wu Li,
and Gerald R. Cunha
6. Serosal Thickening, Smooth Muscle Cell Growth, and Phenotypic Changes in
the Rabbit Bladder Wall during Outflow Obstruction and Regeneration 63
Saverio Sartore, Marleen Roelofs, Angela Chiavegato, Luigi Faggian, and
Rafaella Franch
7. Replicative Senescence in Human Uroepithelial Cells ..................... 83
Jairaj A. Puthenveettil, Melissa S. Burger, and Catherine A. Reznikoff
xiii
xiv Contents
8. Ultrastructural Smooth Muscle Ontogeny of the Rat Bladder. . . . . . . . . . . . . . . . 93
Hsi-Yang Wu, Laurence S. Baskin, Carrie Blakey, Joseph Goodman, and
Gerald R. Cunha
Discussion: Epithelial-Mesenchymal Interactions in the Bladder . . . . . . . . . . . . . . . . . . 103
Section II. Muscle and Extracellular Matrix in the Bladder
Overview of Muscle and Extracellular Matrix in the Bladder. . . . . . . . . . . . . . . . . . . . . 117
Edward J. Macarak
9. Fetal Bladder Physiology ............................................ 121
Hiep T. Nguyen and Barry A. Kogan
10. Developmental Aspects of the Contractile Smooth Muscle Component in Small
Intestinal Submucosa Regenerated Urinary Bladder .................. 129
Bradley P. Kropp
11. Contractile Protein Changes in Urinary Bladder Smooth Muscle following
Outlet Obstruction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137
Samuel Chacko, Michael DiSanto, Chandrakala Menon, Yongmu Zheng,
Joseph Hypolite, and Alan J. Wein
12. Calcium Ion Homeostasis in Urinary Bladder Smooth Muscle. . . . . . . . . . . . . . . 155
Stephen A. Zderic, Chao liang Gong, Mike Desanto, Joseph Hypolite,
Joel Hutcheson, Alan J. Wein, and Samuel Chacko
13. Cyclooxygenase-2: A Key Regulator of Bladder Prostaglandin Formation 171
John M. Park, Jiirgen B. Schnermann, and Josephine P. Briggs
14. Role of Angiotensin II in Bladder Smooth Muscle Growth and Function 183
Earl Y. Cheng, Robert S. Decker, and Chung Lee
15. New Concepts on the Normal and Abnormal Developing Bladder . . . . . . . . . . . . 193
Ellen Shapiro
16. Biochemical and Physiological Characterization of the Urinary Bladder in
Ehlers-Danlos Syndrome ....................................... 201
Catherine M. Deveaud, William A. Kennedy II, Stephen A. Zderic, and
Pamela S. Howard
17. The Role of Collagen in Bladder Filling ................................ 215
Edward J. Macarak and Pamela S. Howard
Discussion: Muscle and Extracellular Matrix in the Bladder 225
Section III. Nerves and Pharmacology in the Bladder
Overview of Nerves and Pharmacology in the Bladder. .. . . . . . . . . . . .. . .. . . . . . . . . 237
Robert M. Levin
