Table Of ContentDiagnostic Bone Marrow
Haematopathology
Diagnostic Bone Marrow
Haematopathology
Jon van der Walt, MB, BCh, FRCPath
Guy’sandStThomas’Hospitals,London
Attilio Orazi, MD, FRCPath
TexasTechUniversityHealthSciencesCenter,ElPaso,Texas
Daniel A Arber, MD
UniversityofChicago,Chicago,Illinois
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Informationonthistitle:www.cambridge.org/9781107145009
DOI:10.1017/9781316535042
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Contents
List of Contributors vi
Preface ix
Acknowledgements x
1. The Bone MarrowBiopsy 1 12. Systemic Mastocytosis 181
Jon vanderWalt Tracy GeorgeandAmy Jencks
2. The NormalBone Marrow 14 13. MyeloidandLymphoidNeoplasmsAssociated
Anna Green with Eosinophilia 200
Sa Wang and RobertoMiranda
3. Necrosis,StromalChanges and Artefacts 26
KonnieHebeda 14. PrecursorLymphoid Neoplasms 231
Girish Venkataraman
4. Aplasia 42
Olga Pozdnyakovaand Emily Mason 15. MatureLymphoid Neoplasms 245
AyomaAttygalle and Sunil Iyengar
5. Hyperplasia 64
Lívia Rásó-Barnett 16. Plasma Cell Neoplasia 283
Falko Fend
6. Infective, Granulomatous andBenign
HistiocyticDisorders 79 17. Metastatic Lesions 298
Alexandar Tzankov Hesham ElDalyand MariamAmer
7. Malignant Disorders of the Histiocytic/ 18. BoneMarrowChangesFollowingTherapyand
Dendritic Lineage 98 Immunosuppression 314
Monika Klimkowska Alexandar Tzankov
8. MyelodysplasticSyndromes 107 19. ImmunohistochemistryandFlow Cytometry
Olga Weinberg and Robert Hasserjian in Bone Marrow Haematopathology 340
Anna Porwit and Mats Ehinger
9. Acute MyeloidLeukaemia 127
KaarenReichard and DanielAArber 20. Molecular Diagnostics in Bone Marrow
Haematopathology 362
10. MyeloproliferativeNeoplasms 146
Mats Ehinger, Per Levéen and Anna Porwit
Hans Michael Kvasnickaand Attilio Orazi
11. Myelodysplastic/Myeloproliferative
Neoplasms 162
Julia Geyer andAttilio Orazi Index 418
v
Contributors
Mariam Amer MBBCH, MSc Robert Hasserjian MD
Haematology andClinical Pathology, Cairo MassachusettsGeneral Hospital, Hematopathology
University,Cairo, Egypt Fellowship Program, PathologyService, Harvard
Medical School,MA, USA
DanielA Arber MD
Department of Pathology,University of Chicago, Konnie Hebeda MD, PhD
Chicago, IL, USA Department of Pathology,Radboud University
Medical Center,Nijmegen, The Netherlands
Ayoma AttygalleMBBS, FRCPath, PhD
Specialist Integrated HaematologicalMalignancy Sunil Iyengar MBBS, MRCP, FRCPath, PhD
Diagnostic Service (SIHMDS), Centre for Molecular Specialist Integrated HaematologicalMalignancy
Pathology,Royal MarsdenHospital, Surrey, UK Diagnostic Service (SIHMDS), Centre for Molecular
Pathology,Royal MarsdenHospital, Surrey, UK
Mats EhingerMD, PhD
Department of Pathologyand Oncology, Institution Amy Jencks DO
ofClinical Sciences,Lund University,Lund, DepartmentofPathology,UniversityofNewMexico,
Sweden NM, USA
Hesham El Daly MBBCH, MSc, MSc, PhD,FRCPath Monika Klimkowska MD, PhD
Haematopathology OncologyDiagnostic Service, Department of Clinical Pathologyand Cytology,
CambridgeUniversityHospitals,Cambridge,UKand Karolinska University Hospital, Stockholm,Sweden
Clinical Pathology, Cairo University,Cairo, Egypt
Hans MichaelKvasnicka MD, PhD
FalkoFend MD, PhD Institute of Pathology and MolecularPathology,
InstituteofPathologyandNeuropathology,Reference Helios University ClinicWuppertal, University of
Center for Hematopathology, University Hospital Witten/Herdecke, Heusnerstr. 40, 42283Wuppertal,
Tuebingen,Eberhard-Karls-University, Tuebingen, Germany
Germany
Per Levéen PhD
Tracy GeorgeMD Department of Pathologyand Oncology, Institution
Clinical Affairs Hematopathology,Departmentof ofClinical Sciences,Lund University,Lund, Sweden
Pathology,UniversityofNewMexicoHealthSciences
Emily Mason MD, PhD
Center, NM, USA
Department of Pathology,Microbiology, and
JuliaGeyer MD Immunology, Vanderbilt University Medical Center,
Department of Pathologyand Laboratory Medicine, Nashville, TN,USA
Weill Cornell Medical College, New York, NY, SA
Roberto Miranda MD
Anna Green BA,MBBS, FRCPath Department of Hematopathology, Division of
Department of Cellular Pathology,St Thomas’ Pathology/Lab Medicine, The University ofTexas
Hospital, Westminster Bridge Road, London, UK MDAnderson CancerCenter,Houston, TX, USA
vi
Contributors
Attilio Orazi MD, FRCPath Alexandar Tzankov MD, PhD
Department of Pathology atTexasTech HistopathologyandAutopsy,UniversityHospital
University HealthSciences Center, Paul Basel,PathologySchoenbeinstrasse,Basel,Switzerland
L. Foster Schoolof Medicine, ElPaso,
Jon vander Walt MB, BCh, FRCPath
Texas, USA
Histopathology, Guy’s and StThomas’Hospitals,
Anna Porwit MD, PhD London,UK
Department of Pathology andOncology, Institution
Girish Venkataraman MD
of Clinical Sciences,LundUniversity,Lund,
ImmunohistochemistryLab,Departmentof
Sweden
Pathology, Section of Hematopathology, The
Olga PozdnyakovaMD,PhD University of Chicago Medicine, Chicago, IL, USA
Harvard Medical School, Brigham and Women's
Sa Wang MD
Hospital, Boston, MA, USA
Divisionof Pathology/Lab Medicine, The University
Lívia Rásó-Barnett MBBCH, PhD,FRCPath of Texas MD AndersonCancer Center, and
Haematopathology, Addenbrooke’s Hospital, Department of HospitalFlow Cytometry Laboratory,
Cambridge, UK The University of Texas MD Anderson Cancer
Center,Houston,TX, USA
Kaaren Reichard MD
Department of Laboratory Medicine and Pathology, Olga WeinbergMD
Divisionof Hematopathology, Mayo Clinic, DepartmentofPathologyBostonChildren’sHospital,
Rochester,MN, USA Boston,MA, USA
vii
Preface
The use of a bone marrow trephine biopsy has a LymphoidTissues,whichwaspublishedinSeptember
venerable history, the first diagnostic biopsy dating 2017. The WHO classification mandates the integra-
backtothebeginningofthetwentiethcentury.How- tion of clinical, morphological, immunological and
ever, the trephine biopsy only entered mainstream molecular diagnosis into a final report in which all
practice in the 1950s with the development of the the modalities are integrated. This book, Diagnostic
modern biopsy technique, using the right posterior Bone Marrow Haematopathology, brings together
iliac crest. Initially, analysis was largely morpho- multiple authors all of whom are specialist haemato-
logical, using either haematoxylin and eosin or pathologistsbasedinEuropeandtheUnitedStatesof
Giemsa type stains, assisted by tinctorial stains. The America.The20chaptersfollowtheWHOclassifica-
finer details of the marrow were obtained from the tion and the multimodality diagnostic approach is
aspiratesmearsandthetwotechniqueswerecomple- emphasized throughout. The importance of optimal
mentary. New players on the scene arrived in the processing of biopsies for the molecular era and the
shape of immunohistochemistry, which allowed need for quality control in the age of personalised
accurateidentificationofcellsinthebiopsy,andflow medicine is highlighted. The book concludes with
cytometry, which performed a similar function for chapters describing immunological and molecular
the peripheral blood and aspirate. Most recently, a diagnostic techniques used in modern
wide range of molecular diagnostic techniques has haematopathology.
extended the diagnostic repertoire into the very mol- Wehopethisbookwilllivenexttothemicroscope
ecules of the bone marrow, using peripheral blood and that users at all levels of experience and back-
and aspiratedor biopsied marrow. grounds will find it to be a resource for the study of
Not surprisingly, advances in diagnostic tech- the pathology of bone marrow disease as well as a
niques have resulted in advances in the clinically day-to-dayreferenceguidetothedetailsofclassifica-
relevantclassificationofdiseasesofthebonemarrow, tion and diagnosis. To this end, wherever possible
reflected in the 2016 revision to the World Health diagnostic criteria and reference information is pre-
Organization Classification of Haematopoietic and sented in atabular form foreveryday quick access.
ix
