Antibodies for Immunohistochemistry

181 Pages·2012·11.63 MB·English

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Bio SB, Inc. Mission Statement
At Bio SB, Inc., we are proud to develop, manufacture and distribute unique products for
Immunohistochemistry, FISH, CISH, Liquid Cytology and PCR technologies that meet the highest
international standards for applications in Molecular Pathology. We have a talented, dedicated, and
vibrant team of professionals that excel in the R&D, Production and Marketing of Immunochemicals
for Cancer Research, Microbiology, Immunology and Genetics. BIO SB manufactures and develops
products in accordance with FDA QSR 21 CFR Part 820 cGMP. These guidelines enable us to produce
an IVD product that meets the highest in vitro diagnostic standards.
Our mission is to develop high-quality immunochemicals while providing outstanding technical
service to our customers. We develop rabbit and mouse monoclonal antibodies and detection
systems for Immunohistochemistry and Immunocytochemistry using proprietary technologies.
Our ImmunoDetector, PolyDetector and CytoDetector systems are unique, high-sensitivity detection systems already in use in
laboratories worldwide. Our Liquid Cytology offers an affordable, easy-to-use alternative to the traditional Pap smear.
Our strategic alliance with ZytoVision of Germany has added a complete range of products for chromogenic (CISH) and florescent
in situ hybridization (FISH). These technologies are designed for the evaluation and detection of genetic aberrations such as
translocations, deletions, amplifications, chromosomal aneuploidy and gene amplifications. We also offer outstanding fast detection
and discrimination of human pathogenic viruses in formalin-fixed, paraffin-embedded tissue sections, cell samples, blood or bone
marrow smears, and metaphase chromosome spreads.
We are very proud to share with our partners and customers our association with Celerus Diagnostics of Carpinteria, CA that will enable
us to offer our customers with the most sophisticated automated system for Immunohistochemistry (IHC): The Wave RPD System.
The Wave RPD System performs totally automated, rapid IHC in one hour. The sophisticated features and innovative components of the
Wave RPD System combine for standardized results, ease-of-use and high-quality staining to accelerate patient care.
Our partnership with Maxim Biotech of Rockville, MD has expanded our capacity to provide our customers with single, dual and
multiplex PCR technologies for Nucleic Acid Amplification. Maxim’s core competency, the development of multiplex amplification-assay
systems, affords researchers the opportunity to significantly increase assay through-put while reducing the typical time required for
many of today’s amplification-assay systems. Maxim’s diverse line of kits and services is designed to make molecular biology research
techniques faster, more cost- effective, and more accessible to researchers working in a broad range of disciplines.
Our alliance with Shanghai ZJ Bio-Tech Co. Ltd., of Shanghai, China, has expanded our range of products for Real Time PCR. Shanghai
ZJ Bio-Tech Co. Ltd., is a leading Diagnostics company, focused on developing and manufacturing Real Time PCR Diagnostic reagents
with applications in Microbiology, Molecular Biology, Cell Biology, and Immunology. Complete PCR kits for Hepatitis Virus, HIV,
Sexually- transmitted Diseases, Respiratory Diseases (Including complete kits for Influenza A H1N1), Infectious Diarrhea, Organ
Transplantation, Insect Vector Diseases and Zoonosis Diseases are effectively used in clinical diagnosis, public health, entry-exit
inspection and quarantine, food safety inspection, and animal husbandry.
At Bio SB our passion is providing biomedical laboratories with the tools to improve the diagnosis, prognosis and therapies that benefit
patients worldwide.

Dr. Alfonso Heras
President & CEO
[email protected] • www.biosb.com

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BIO SCIENCE FOR THE WORLD
Bio SB
Immunopathology
• Rabbit and Mouse Antibodies for Immunohistochemistry (IHC)
• HRP & AP Detection Systems for IHC
• Heat Epitope Retrieval Solutions
• Substrate-chromogens for HRP & AP
• Equipment and Ancillaries
• CytoLayer Liquid Based Cytology
Shanghai ZJ Bio-Tech
Kits for Real Time PCR
• Bacterial and Viral Load Quantification • SNP Genotyping
• Pathogen Detection & Typing • Validation of RNAi
• Differential Gene Expression • Forensic Studies
• Therapeutic Drug Monitoring
• Genetic Disease Analysis
• Food & Environmental Pathogen Detection
Celerus Diagnostics
Totally Automated IHC in One Hour!
• Rapid IHC in one hour
• Standard Results, ease-of-use and high-quality IHC
• Limitless Flexibility
• Lean Process Maximized
• Smarter, Faster-Rapid IHC
[email protected] • www.biosb.com
ZytoVision
CISH and FISH
ZytoLight – Products for FISH
HER2 | EGFR | MDM2 | ESR1 | MEC | RMS | FHIT | p16 | CCND1 | FGFR1 | nMYC | cMYC
PTEN | MDM4 | FGFR3 | MET | TERT | EWSR1 | SYT | CHOP | CDK4 | ALK/EML4
ZytoLight® products for Fluorescence in situ Hybridization (FISH) are designed for the identification
of genetic aberrations such as translocations, deletions, amplifications, and chromosomal aneuploi-
dies associated with tumors and genetic diseases.
ZytoDot – Products for CISH
HER2 | FGR1 | TOPO2A | EGFR | cMYC | XY | MDM2 | cMYC | TOPOZA | FGR1
ESR1 | nMYC | CCND1
ZytoDot® products for Chromogenic in situ Hybridization (CISH) are designed for the detection of
aneuploidies and gene amplifications associated with tumors and genetic disease using an IHC-like
procedure and light microscopy.
ZytoFast – Products for CISH
HPV | EBV | CMV | Ig-Kappa | Ig-Lambda
ZytoFast® products for Chromogenic in situ Hybridization (CISH) are designed for the detection and
discrimination of human pathogen viruses such as HPV, EBV, CMV, and the determination of lymphocyte
clonality by detecting Ig-κ and Ig-λ light chains RNA.
Ancillaries for FISH & CISH
Equipment, High quality detection substrates, chromogens, essential washing solutions and control
slides for CISH and FISH.
Maxim Biotech
Single, Dual and Multiplex PCR
Complete kits for single, dual and multiplex PCR
Nucleic Acid Extraction Kits Detection and
quantification kits for
• Interleukins
• Apoptosis
• Receptors
• Growth Factors
• Oncogenes
• Genetic Diseases
• Microorganisms
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New Rabbit Monoclonal Antibodies
from Bio SB, Inc.
Higher Affinity | Higher Sensitivity | Higher Specificity
• ALK-1, RBT-ALK1 • CD5, RBT-CD5 • CK17, EP98 • ER, RBT11 • PAX-5, RBT-PAX-5
• AMACRacemase, RBT-AMACR • CD21, EP64 • CK19, EP72 • HER-2 neu, RBT-HER2 • PLAP, EPR6141
• Calretinin, EP1798 • CD117, YR145 • Cyclin D1, RBT14 • Ki67, EP5 • PR, RBT22
• CD1a, EP80 • CDX-2, EP25 • DOG-1, RBT-DOG1 • Mammaglobin, 31A5 • PSMA, EPR6253
• CD3, RBT-CD3 • COX-2, RBT-COX2 • E-Cadherin, EP700Y • MCM2, RBT-MCM2 • Topoisomerase IIa, RBT-Topo2a
• CD4, RBT-CD4 • VEGF, RBT-VEGF
Bio SB, Inc., 69 Santa Felicia Dr., Santa Barbara, CA 93117, USA
Tel: [phone] | Tel: [phone] | Fax: [phone]
E-mail: [email protected] | Website: www.biosb.com
[email protected] • www.biosb.com
Table of Contents
Antibodies for Immunohistochemistry .................. 7
Antibodies ............................................ 8
Antibodies for Immunofluorescence .................... 71
Detection Systems for IHC ................................73
ImmunoDetector HRP Detection Systems ......................74
ImmunoDetector AP Detection Systems .......................75
PolyDetector HRP Detection Systems ..........................76
Mouse/Rabbit PolyDetector HRP w/DAB .......................76
Mouse/Rabbit PolyDetector HRP w/AEC .......................76
Mouse PolyDetector HRP w/DAB ..............................76
Mouse PolyDetector HRP w/AEC ...............................76
Rabbit PolyDetector HRP w/DAB ...............................76
Rabbit PolyDetector HRP w/AEC ...............................76
Complete Detection Systems ..................................77
HPV CytoDetector HRP/DAB ...................................77
7
HER2 neu PolyDetector HRP/DAB ..............................78
ER/PR PolyDetector HRP/DAB. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79
CD117 c-Kit PolyDetector HRP/DAB ............................80
71
Substrate Chromogen Systems ........................ 81
HRP Substrate Chromogen ....................................82
ImmunoDetector Liquid AEC Ready-To-Use ....................82
ImmunoDetector Liquid DAB kit ...............................82
ImmunoDetector DAB Buffer ..................................82
ImmunoDetector DAB Chromogen ............................82
PolyDetector HRP Black kit ....................................82
AP Substrate Chromogen .....................................83
PolyDetector Alk Blue Ready-To-Use ...........................83
PolyDetector Alk Red Ready-To-Use ............................83
PolyDetector Alk Brown Ready-To-Use .........................83
PolyDetector Alk Magenta .....................................83
Ancilliaries ........................................... 85
ImmunoDNA Retrievers .......................................86
ImmunoDNA Retriever 20X with Citrate ........................86
ImmunoDNA Retriever 20X with EDTA .........................86
Protein Block / Antibody Diluent ...............................86
Negative Controls .............................................86
Immuno/DNA Washer 10X ....................................86
PolyDetector HRP and AP Buffers ..............................86
Tween 20 .....................................................86
Mounting Media ..............................................86
Aqua Mounter ................................................86
Perma Mounter ...............................................86
PolyDetector Peroxidase Block ................................86
PolyDetector AP Block ........................................86
81
ImmunoDetector Biotin Blocker ...............................86
ImmunoDNA Background Blocker .............................86
HybriWash 20X ...............................................86
Hematoxylin Counterstainer ...................................86 85
Nuclear Fast Red Counterstainer ...............................86
Methyl Green Counterstainer ..................................86
Analyte Specific Reagent
For in Vitro Diagnostic Use Analytical and performance characteristics are not established. For Research Use Only
[email protected] • www.biosb.com
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FISH & CISH Products ................................. 87
FISH-ZytoLight ................................................88
ZytoLight FISH probes ........................................89
ZytoLight FISH Kits ............................................89
CISH-ZytoDot .................................................90
ZytoDot CISH probes ..........................................91
ZytoDot CISH Kits .............................................91
CISH-ZytoFast .................................................92
ZytoFast Probes ...............................................93
ZytoFast Detection Systems ...................................93
Ancillaries for FISH & CISH .....................................94
Equipment and Slides ................................ 95
Wave RPD ....................................................96
Pressure Cooker and Staining Dishes ........................ 103
Digital Pressure Cooker ...................................... 103
Plastic Staining Dish ......................................... 103
Slide Holder, 24 places ...................................... 103
87
Slides ....................................................... 103
Probe On Plus Slides, Box of 72 .............................. 103
Probe On Slides, Box of 72 .................................. 103
Superfrost Plus Slides, Box of 72 ............................. 103 95
Colorfrost Slides, Box of 72 .................................. 103
Capillary-gap Slides width: 130um; Color, Gold ............... 103
Microscope Slides for Liquid Cytology, Box of 72 ............. 103
Hydrophilic Plus Slides for Molecular Pathology .............. 104
Liquid-Based Cytology ...............................109
Equipment ................................................. 111
Gynecological Liquid Cytology .............................. 111
Non-Gynecological Liquid Cytology ......................... 111
Accessories for Liquid Cytology .............................. 111
CytoLayer Fixitive ........................................... 111
Gyn Accessories Cytology Kit ................................ 111
Non-Gyn Accessories Cytology Kit ........................... 111
Cervex Brush ................................................ 111
CytoLayer Sedimentation Chambers ......................... 111
15 ml Conical Centrifuge Tubes .............................. 111
1 ml Transfer Pipettes ....................................... 111
Microscope Slides ........................................... 111
Slide Treatment Solution .................................... 111
Density Gradient ............................................ 111
Lysis Solution ............................................... 111
PCR .................................................113
DNA/RNA Isolation Kits ...................................... 114
RT PCR Kits ................................................. 114
Ancillary Products ........................................... 114
Single & Multiplex PCR ...................................... 115
109
Real Time PCR ............................................... 139
Automated Nucleic Acid Extraction System .................. 154
Molecular Pathology Technical Index ..................157 113
Product Index .......................................174
Ordering Information/Distributors ....................175
Analyte Specific Reagent
For in Vitro Diagnostic Use Analytical and performance characteristics are not established. For Research Use Only
[email protected] • www.biosb.com
Antibodies for Immunohistochemistry
GretchenKing–
Histology
“Our Objective is to develop, manufacture and market easy-to-use, safe,
reliable and economical products for Cancer Prognostics and Diagnostics
that meet the most stringent International standards.”
For Research Use Only
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Alpha-1-Antichymotrypsin A-1-Antitrypsin ACTH
IHC of Alpha-1-Antichymotrypsin on an IHC of Alpha-1- Antitrypsin on an FFPE Tonsil IHC of ACTH on an FFPE Pituitary Tissue
FFPE Tonsil Tissue

Alpha-1-Antichymotrypsin is a glycoprotein found Alpha-1-Antitrypsin (A1AT) is a glycoprotein Adrenocorticotropic Hormone (ACTH or
in the alpha (1)-globulin region in human serum. generally known as serum trypsin inhibitor. corticotropin) is a polypeptide hormone
It inhibits chymotrypsin-like proteinases in vivo Alpha-1-Antitrypsin is also referred to as alpha-1 synthesized from POMC, (Pro-opiomelanocortin)
and has cytotoxic killer-cell activity in vitro. proteinase inhibitor (A1PI) because it is a serine and secreted from corticotropes in the
The protein also has a role as an acute-phase protease inhibitor (serpin), inhibiting a wide anterior lobe of the pituitary gland in response
protein and is active in the control of immuno- variety of proteases. It protects tissues from to Corticotropin-releasing Hormone (CRH)
logic and inflammatory processes, and as a tumor enzymes of inflammatory cells, especially released by the hypothalamus. It consists of 39
marker. It is a member of the serpin superfamily. elastase, and has a reference range in blood amino acids.
of 1.5 - 3.5 gram/liter (in the U.S. the reference
Alpha-1-Antichymotrypsin antibody reacts range is generally expressed as mg/dL or ACTH is a useful marker in the classification of
with histiocytes and histiocytic neoplasms. Its micromoles), but the concentration can rise pituitary tumors and the study of pituitary
major application is defining the presence of many fold upon acute inflammation. In its disease. It reacts with ACTH-producing cells
Alpha-1-Antichymotrypsin in histiocytes and absence, elastase is free to break down elastin, (corticotrophs), as well as other tumors (e.g.,
tumors derived from them. In eosinophilic which contributes to the elasticity of the lungs, some Small-Cell Carcinomas present in lung
granuloma and malignant histiocytosis, the resulting in respiratory complications such as tissue) causing Paraneoplastic Syndromes by
reaction for this marker is heterogeneous in emphysema, or COPD (Chronic Obstructive secreting ACTH.
intensity and distribution. In fibrous histiocy- Pulmonary Disease) in adults and cirrhosis in
tomas, under certain circumstances, a diffuse adults or children.
homogeneous reaction may be observed.
Alpha-1-Antitrypsin is considered to be very
useful in the study of inherited AAT deficiency,
benign and Malignant Hepatic Tumors and
Yolk-Sac Carcinomas. Positive staining for A-1-
Antitrypsin may also be used in detection of
benign and malignant lesions of a histiocytic
nature. Sensitivity and specificity of the results
have made this antibody a useful tool in the
screening of patients with Cryptogenic Cirrhosis
or other forms of liver disease with portal fibrosis
of unknown etiology.

ANTIBODY TYPE Rabbit Polyclonal A NTIBODY TYPE Rabbit Polyclonal A NTIBODY TYPE Rabbit Polyclonal
C LONE N/A C LONE N/A C LONE N/A
ISOTYPE N /A I SOTYPE N/A I SOTYPE N/A
CONTROL Tonsil, Lymph Node C ONTROL Tonsil, Lymph Node C ONTROL Normal Pituitary
L OCALIZATION Cytoplasmic L OCALIZATION Cytoplasmic L OCALIZATION Cytoplasmic

CAT. # PRESENTATION VOL/QTY CAT. # PRESENTATION VOL/QTY CAT. # PRESENTATION VOL/QTY
BSB 5001 prediluted 3.0 ml BSB 5008 prediluted 3.0 ml BSB 5015 prediluted 3.0ml
BSB 5002 prediluted 7.0 ml BSB 5009 prediluted 7.0 ml BSB 5016 prediluted 7.0 ml
BSB 5003 prediluted 15.0 ml BSB 5010 prediluted 15.0 ml BSB 5017 prediluted 15.0 ml
BSB 5004 concentrated 0.1 ml BSB 5011 concentrated 0.1 ml BSB 5018 concentrated 0.1 ml
BSB 5005 concentrated 0.5 ml BSB 5012 concentrated 0.5 ml BSB 5019 concentrated 0.5 ml
BSB 5006 concentrated 1.0 ml BSB 5013 concentrated 1.0 ml BSB 5020 concentrated 1.0 ml
BSB 5007 control slides 5 BSB 5014 control slides 5 BSB 5021 control slides 5
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